A comparison of post-thaw results between cryopreserved embryos derived from intracytoplasmic sperm injection and those from conventional IVF.

Objective: To study the effect of freezing on early stage embryos derived from intracytoplasmic sperm injection (ICSI) or from IVF.

Design: Prospective, controlled clinical study.

Setting: Private IVF center.

Clinical application of intracytoplasmic sperm injection using in vitro cultured testicular spermatozoa obtained the day before egg retrieval.

Objective: To study the effect of in vitro culture on the quality of human testicular sperm and the efficiency of intracytoplasmic sperm injection with in vitro cultured testicular sperm.

Design: Clinical study.

Setting: A private IVF center.

Maximizing pregnancy rates and limiting higher-order multiple conceptions by determining the optimal number of embryos to transfer based on quality.

Objective: To define statistical thresholds for the number of embryos to be transferred to achieve an optimal pregnancy rate and keep higher-order multiple conceptions (pregnancy with more than two fetal sacs with cardiac activity) within an acceptable limit.

Design: A retrospective review of patient records.

Setting: Private practice assisted reproductive technology (ART) facility.

Coculture of human embryos with buffalo rat liver cells for women with decreased prognosis in in vitro fertilization.

Objective: The coculture of human embryos with epithelial cells may improve both embryo quality and pregnancy rates. In this current study we tested the efficacy of coculture with the buffalo rat liver cell line on pregnancy rates in women with a potentially poor prognosis for success with in vitro fertilization (previous in vitro fertilization failure, advanced maternal age, increased early follicular follicle-stimulating hormone levels, and anovulation).

Study Design: This prospective controlled study evaluated a total of 203 women (135 coculture, 68 controls) undergoing in vitro fertilization.

Clinical application of nonselective assisted hatching of human embryos.

Objective: To evaluate the effect of nonselective assisted hatching on pregnancy rate (PR) and to provide an alternative and simplified method for clinical application of assisted hatching.

Design: Retrospective analysis of clinical data.

Setting: Private infertility practice.

The combination of coculture and selective assisted hatching: results from their clinical application.

Objective: To establish pregnancies using a combination of coculture and selective assisted hatching.

Design: Clinical application for a selected group of patients. Not a controlled study.

Embryonic morphology and rate of implantation of human embryos following co-culture on bovine oviductal epithelial cells.

A study was undertaken to evaluate embryonic development and establish pregnancies with human embryos after in-vitro culture in two different systems. Treatment A consisted of culturing zygotes in serum-supplemented human tubal fluid culture medium (HTF). Treatment B consisted of culturing zygotes on a monolayer of bovine oviductal epithelial cells with HTF.