Genomic investigation of plant secondary metabolism: insights from synteny network analysis of oxidosqualene cyclase flanking genes.

The clustered distribution of genes involved in metabolic pathways within the plant genome has garnered significant attention from researchers. By comparing and analyzing changes in the flanking regions of metabolic genes across a diverse array of species, we can enhance our understanding of the formation and distribution of biosynthetic gene clusters (BGCs). In this study, we have designed a workflow that uncovers and assesses conserved positional relationships between genes in various species by using synteny neighborhood networks (SNN).

Molecular profiles of blood from numerous species that differ in sensitivity to acute inflammation.

Vertebrates differ over 100,000-fold in responses to pro-inflammatory agonists such as bacterial lipopolysaccharide (LPS), complicating use of animal models to study human sepsis or inflammatory disorders. We compared transcriptomes of resting and LPS-exposed blood from six LPS-sensitive species (rabbit, pig, sheep, cow, chimpanzee, human) and four LPS-resilient species (mice, rats, baboon, rhesus), as well as plasma proteomes and lipidomes. Unexpectedly, at baseline, sensitive species already had enhanced expression of LPS-responsive genes relative to resilient species.

Ecotoxicity of Biodegradable Microplastics and Bio-based Microplastics: A Review of in vitro and in vivo Studies.

As biodegradable and bio-based plastics increasingly replace conventional plastics, the need for a comprehensive understanding of their ecotoxicity becomes more pressing. This review systematically presents the ecotoxicity of the microplastics (MPs) from different biodegradable plastics and bioplastics on various animals and plants. High doses of polylactic acid (PLA) MPs (10%) have been found to reduce plant nitrogen content and biomass, and affect the accumulation of heavy metals in plants.

Cultural heritage site selection characteristics and the impact of the natural environment in Jinan city, China.

This study aims to explore the spatial distribution and site selection characteristics of cultural heritage sites, as well as the impact of the natural environment on the site's location. A total of 448 cultural heritage sites in Jinan City (Shandong province), which have been listed as key cultural relic protection units from before the Qin Dynasty to after the Qing Dynasty (ca. 7500 BCE-present), were analyzed using spatial analysis tools in ArcGIS 10.

The dual role of calnexin on malignant progression and tumor microenvironment in glioma.

Glioma is the most common malignant brain tumor. Previous studies have reported that calnexin (CANX) is significantly up-regulated in a variety of malignant tumors, including glioma, but its biological function and mechanism in glioma is still unclear. In this study, differentially expressed proteins in 3 primary glioblastoma multiforme (GBM) tissues and 3 paracancer tissues were identified by liquid chromatography-tandem mass spectrometry-based proteomic and bioinformatic analysis.

Impacts of planting structure adjustment on water saving in the Shiyang River Basin of Arid Region.

Planting structure adjustment (PSA) affects agricultural water saving, and is an essential part of water-saving agricultural construction. This study introduced virtual water theory and innovatively constructed a model to assess the water-saving effects of PSA in Shiyang River Basin over the past 38 years, explore the relationship between planting structure and water saving, and clarify the most water-saving planting structure. The results showed that the sown area of economic crops consistently increased as food crop areas decreased in the four counties (districts) from 1980 to 2017.

Evaluation of wheat drought resistance using hyperspectral and chlorophyll fluorescence imaging.

Photosynthesis drives crop growth and production, and strongly affects grain yields; therefore, it is an ideal trait for wheat drought resistance breeding. However, studies of the negative effects of drought stress on wheat photosynthesis rates have lacked accurate evaluation methods, as well as high-throughput techniques. We investigated photosynthetic capacity under drought stress in wheat varieties with varying degrees of drought stress resistance using hyperspectral and chlorophyll fluorescence (ChlF) imaging data.

In search of pathogenic Leptospira species in Myotis and other vesper bats, Russia.

Various bat species worldwide have been identified as Leptospira carriers, especially in tropical regions. In this study, we investigated the infection of Vespertilionidae bats by pathogenic Leptospira in north-west Russia. Out of 264 bats from 13 species, the urine of 24 specimens tested positive according to a polymerase chain reaction test.

Flexible progestin-primed ovarian stimulation versus a GnRH antagonist protocol in predicted suboptimal responders undergoing freeze-all cycles: a randomized non-inferiority trial.

Study Question: Are live birth rates (LBRs) per woman following flexible progestin-primed ovarian stimulation (fPPOS) treatment non-inferior to LBRs per woman following the conventional GnRH-antagonist protocol in expected suboptimal responders undergoing freeze-all cycles in assisted reproduction treatment?

Summary Answer: In women expected to have a suboptimal response, the 12-month likelihood of live birth with the fPPOS treatment did not achieve the non-inferiority criteria when compared to the standard GnRH antagonist protocol for IVF/ICSI treatment with a freeze-all strategy.

What Is Known Already: The standard PPOS protocol is effective for ovarian stimulation, where medroxyprogesterone acetate (MPA) is conventionally administered in the early follicular phase for ovulatory suppression. Recent retrospective cohort studies on donor cycles have shown the potential to prevent premature ovulation and maintain oocyte yields by delaying the administration of MPA until the midcycle (referred to as fPPOS), similar to GnRH antagonist injections.

Mettl3-Mediated m6A Modification is Essential for Visual Function and Retinal Photoreceptor Survival.

Purpose: N6-methyladenosine (m6A) modification, one of the most common epigenetic modifications in eukaryotic mRNA, has been shown to play a role in the development and function of the mammalian nervous system by regulating the biological fate of mRNA. METTL3, the catalytically active component of the m6A methyltransferase complex, has been shown to be essential in development of in the retina. However, its role in the mature retina remains elusive.

f. sp. Exhibited a Significant Change in Virulence and Race Frequency in Xinjiang, China.

Xinjiang is an important region due to its unique epidemic characteristics of wheat stripe rust disease caused by f. sp. .

Genome Sequencing and Metabolic Potential Analysis of .

is an edible and medicinal macrofungus with significant biological activity and broad pharmaceutical prospects that has received increasing attention in recent years. Although it is an important resource for macrofungi, knowledge of it remains limited. In this study, we sequenced, de novo assembled, and annotated the whole genome of using a PacBio Sequel II sequencer.

Effects of ECMF Isolated from Mining Areas on Water Status, Photosynthesis Capacity, and Lead Ion Transport of Under Pb Stress.

Native ectomycorrhizal fungi (ECMF) are generally more effective than non-native ECMF in facilitating the phytoremediation of heavy metal (HM) ions from contaminated soils. This study aimed to investigate the contributions of four ECMF species-, , , and -that were isolated from mining areas to the growth, water status, photosynthesis, and metallothionein gene expression of exposed to varying concentrations of lead (Pb). The experiment lasted two months and involved .

Understanding the Second Victim Phenomenon Among Healthcare Workers in an Italian Hospital.

Second victim syndrome (SVS) refers to the psychological trauma experienced by healthcare workers (HCWs) as a result of being involved in an adverse event (AE). Research on the prevalence of SVS and the support needed for HCWs who experience it is limited. A cross-sectional study was conducted at the Health Local Unit of Lecce, in Puglia, to identify the phenomenon of SVS among HCWs and recognize the forms of support received and desired.

Engineering an Ionic Aggregation-Induced Luminescence-Labeled Fluorescence Lateral Flow Immunoassay for C-Reactive Protein in Human Plasma.

The surge of lateral flow immunoassays (LFAs) stimulates researchers to explore the novel vibrant aggregation-induced emission luminogen (AIEgen)-doped nanoparticles to improve the accuracy and reliability of LFAs. However, the loading amount of AIEgens currently used for the LFA in microspheres is limited due to their symmetrical large conjugated skeleton structure, which significantly reduces the fluorescence brightness of the signal reporter in the LFA. Herein, an ionic AIEgens with a donor-acceptor type was developed as the signal reporter of the LFA for C-reactive protein (CRP).

[Land use changes and driving forces of landslide area in Tongwei County, Gansu Province, Northwest China from 1985 to 2020.].

Accurately analyzing the type of land use and change characteristics of disaster damage in landslide areas is of great significance to scientifically promote the optimization of regional land use pattern and disaster prevention and mitigation. We analyzed the characteristic parameters of landslides as well as the characteristics and driving factors of land use change from 1985 to 2020 in Tongwei County, Gansu Province, using ALOS DEM data and 1985-2020 land use data, GIS spatial analysis, land-use dynamic attitude, transfer matrix, and Geodetector. The results showed that a total of 1012 landslide samples were identified, characterized by medium elevation, gentle gradient, low elevation difference, short length, and small size.

[Impact of climate change on the potential geographical distribution of subsp. ].

subsp. is an important resource plant with considerable medicinal, economic, and ecological value, and an indicator species in the transition zones between forests and grasslands. Predicting the potential geographic distribution of subsp.

[Spatiotemporal dynamics of forest cover and its response to climate change in Shandong Province, China during 2000-2022.].

Accurately capturing the spatiotemporal dynamics of regional forest cover and its response to climate change is of great significance for forest resource management and ecological environment protection. We used statistical methods such us linear regression and correlation analysis, as well as remote sensing change monitoring to investigate the spatiotemporal dynamics of forest cover and its response to climate change from 2000 to 2022 in Shandong Province based on MODIS VCF products and meteorological data. The results showed that the forest co-verage and forest area in Shandong Province increased from 43.

[Isolation, screening of zinc solubilizing microorganisms and its application in low zinc calcareous soil].

Inoculating zinc solubilizing microorganisms (ZSMs) is considered as a promising strategy for increasing Zn phytoavailability in soils with low Zn availability. In present study, we screened six strains of ZSMs from rhizosphere of green manure crop, including three strains of fungi, , and three strains of bacteria, . We conducted a pot experiment of Bok choy inoculated with different ZSMs to analyze the Zn content in shoots and roots, and compared the Zn solubilizing effect of ZSMs.

[Impacts of freeze-thaw process on soil microbial nutrient limitation in slope farmlands of the Chinese Mollisol region].

Understanding the impacts of freeze-thaw action on soil microbial nutrient limitation can provide important support for sustainable utilization of black soil resources. We analyzed the impacts of freeze-thaw action on soil microbial nutrient limitation on a slope farmland located in a typical thick Mollisol region of Keshan County, Heilongjiang Province. We examined the responses of soil microbial nutrient limitation to soil erosion rates through measuring soil nutrient, soil microbial biomass, and soil enzyme activities before and after freeze-thaw under natural conditions, and estimated the soil erosion rates by Cs tracing technology.

[Effects of different tillage measures on soil organic carbon in dryland wheat fields on the Loess Plateau, Northwest China based on meta-analysis].

To evaluate the effects of tillage measure on soil organic carbon (SOC) and influence degree of various factors on relative change rate of SOC at regional scale, we conducted a meta-analysis to investigate the impacts of tillage measures (CK, traditional deep tillage without straw return; NTS, no tillage with straw return; NT, no tillage without straw return; TS, traditional tillage with straw return; SS, subsoiling tillage) on SOC content and influence factors (climate conditions, soil types, cultivation types, and initial soil physicochemical properties) on relative change rate of SOC in dryland wheat fields on the Loess Plateau, based on literatures published during 2000-2023. Results indicated that NT, NTS, SS and TS performed varies positive effect on SOC content in 0-20 cm soil layer compared with CK. In addition, greater enhancement of SOC were obtained in conditions of loessal soil, mid-temperate zone, average annual temperature of ≤10 ℃ and average annual rainfall of ≤500 mm.

[Characteristics of soil carbon, nitrogen, and water of plantation along different vegetation restoration years in alpine sandy region].

As the most effective way to remedy and reconstruct the degraded ecosystems, vegetation restoration could affect soil carbon and nitrogen cycles and water balance. We examined the responses of carbon, nitrogen, and water in 0-200 cm soil layer to vegetation restoration years by analyzing their distribution characteristics across a restoration chronosequence of plantation (5, 10, 15, 20, and 25 years) in alpine sandy region of the Qinghai-Tibetan Plateau. The results showed that the content and storage of soil organic carbon (SOC) and soil total nitrogen (STN) increased significantly, while that of soil inorganic carbon (SIC) decreased significantly with restoration years.

Multi-Task Deep Neural Networks for Irregularly Sampled Multivariate Clinical Time Series.

Multivariate clinical time series data, such as those contained in Electronic Health Records (EHR), often exhibit high levels of irregularity, notably, many missing values and varying time intervals. Existing methods usually construct deep neural network architectures that combine recurrent neural networks and time decay mechanisms to model variable correlations, impute missing values, and capture the impact of varying time intervals. The complete data matrices thus obtained from the imputation task are used for downstream risk prediction tasks.

Genomic identification of the gene family in apple and functional analysis of involved in flowering transition.

Unlabelled: Apple is a crucial economic product extensively cultivated worldwide. Its production and quality are closely related to the floral transition, which is regulated by intricate molecular and environmental factors. () is a transcription factor that is involved in regulating plant growth and development, with certain play significant roles in regulating flowering.

Influence of inhaled nitric oxide on bronchopulmonary dysplasia in preterm infants with PPHN or HRF at birth: a propensity score matched study.

Background: Bronchopulmonary Dysplasia (BPD) is a chronic lung disease affecting preterm infants, with limited prevention and treatment options. Inhaled Nitric Oxide (iNO) is sometimes used to treat Persistent Pulmonary Hypertension of the Newborn (PPHN) and Hypoxemic Respiratory Failure (HRF), and its impact on BPD development remains debated.

Objective: To assess whether iNO-related factors are potential contributors to the development of BPD Grade Ⅱ-Ⅲ in very premature infants (VPI) diagnosed with PPHN or HRF at birth using Propensity Score Matching (PSM).