Investigating the ecological fallacy through sampling distributions constructed from finite populations.

Correlation coefficients and linear regression values computed from group averages can differ from correlation coefficients and linear regression values computed using individual scores. This observation known as the ecological fallacy often assumes that all the individual scores are available from a population. In many situations, one must use a sample from the larger population.

Linear Regression of Sampling Distributions of the Mean.

We show that the simple and multiple linear regression coefficients and the coefficient of determination R computed from sampling distributions of the mean (with or without replacement) are equal to the regression coefficients and coefficient of determination computed with individual data. Moreover, the standard error of estimate is reduced by the square root of the group size for sampling distributions of the mean. The result has applications when formulating a distance measure between two genes in a hierarchical clustering algorithm.

Quantitative analyses of T cell motion in tissue reveals factors driving T cell search in tissues.

T cells are required to clear infection, and T cell motion plays a role in how quickly a T cell finds its target, from initial naive T cell activation by a dendritic cell to interaction with target cells in infected tissue. To better understand how different tissue environments affect T cell motility, we compared multiple features of T cell motion including speed, persistence, turning angle, directionality, and confinement of T cells moving in multiple murine tissues using microscopy. We quantitatively analyzed naive T cell motility within the lymph node and compared motility parameters with activated CD8 T cells moving within the villi of small intestine and lung under different activation conditions.

The NEURON Program: Utilizing Low-Cost Neuroscience for Remote Education Outreach.

The NEURON initiative (Neuroscience Education in Undergraduate Research, Outreach, and Networking) is a free program engaging first year students, including underrepresented minority (URM) students in Neuroscience and Cognitive Science (NSCS) at the University of Arizona (UA). The NEURON program builds on former Grass Foundation-sponsored workshops run by Dr. Ricoy (2010-2019) implementing hands-on and culturally responsive active learning curriculum with low-cost equipment from Backyard Brains to increase student retention of URM students in the sciences at Hispanic Serving Institutions (HSI).

Analysis of the annual pollen integral in Albuquerque, New Mexico, shows a negative trend with temperatures for Juniper, Cottonwood, Elm, and Mulberry.

The goal of this study is to determine if the annual pollen integral (APIn) for the top tree allergens in the City of Albuquerque is correlated with meteorological variables. This analysis would be the first of its kind for this area. We used 17 consecutive years from 2004 to 2020 and data collected by the city of Albuquerque using a Spore Trap (Burkard) volumetric air sampler in a location designed to represent a typical desert environment.

Developing and Implementing Low-Cost Remote Laboratories for Undergraduate Biology and Neuroscience Courses.

The global pandemic caused by the novel coronavirus (SARS-COV-2) has forced many universities to abruptly change the delivery of courses from in-person to online. This change to remote learning requires creating new ways to deliver lectures, exams, and discussion groups through online meeting platforms. An often-overlooked challenge is performing lab courses that require access to specialized equipment and resources typically found in the undergraduate laboratory classrooms.

A low-cost computational approach to analyze spiking activity in cockroach sensory neurons.

Undergraduates use a spike sorting routine developed in Octave to analyze the spiking activity generated from mechanical stimulation of spines of cockroach legs with the inexpensive SpikerBox amplifier and the free software Audacity. Students learn the procedures involved in handling the cockroaches and recording extracellular action potentials (spikes) with the SpikerBox apparatus as well as the importance of spike sorting for analysis in neuroscience. The spike sorting process requires students to choose the spike threshold and spike selection criteria and interact with the clustering process that forms the groups of similar spikes.

Modeling the Influence of Mites on Honey Bee Populations.

The mite has been associated with the recent decline in honey bee populations. While experimental data are crucial in understanding declines, insights can be gained from models of honey bee populations. We add the influence of the mite to our existing honey bee model in order to better understand the impact of mites on honey bee colonies.

Describing the Pearson distribution of aggregate data.

Ecological studies and epidemiology need to use group averaged data to make inferences about individual patterns. However, using correlations based on averages to estimate correlations of individual scores is subject to an "ecological fallacy". The purpose of this article is to create distributions of Pearson correlation values computed from grouped averaged or aggregate data using Monte Carlo simulations and random sampling.

Relearning functional and symmetric walking after stroke using a wearable device: a feasibility study.

Background: Gait impairment is a common consequence of stroke and typically involves a hemiparetic or asymmetric walking pattern. Asymmetric gait patterns are correlated with decreased gait velocity and efficiency as well as increased susceptibility to serious falls and injuries.

Research Question: This paper presents an innovative device worn on a foot for gait rehabilitation post stroke.

Adipose tissue derived mesenchymal stem cells are better respondents to TGFβ1 for in vitro generation of cardiomyocyte-like cells.

Mesenchymal stem cells (MSCs) are multipotent cells which hold immense potential in translational research as a novel treatment modality. In recent years, MSCs isolated from various tissues have been used in several clinical trials for the treatment of cardiac injury caused by permanent myocardial loss. However, a better MSCs source and an optimum inducer for in vitro cardiac differentiation are still far reaching and unexplored.

Interstitial Migration of CD8αβ T Cells in the Small Intestine Is Dynamic and Is Dictated by Environmental Cues.

The migratory capacity of adaptive CD8αβ T cells dictates their ability to locate target cells and exert cytotoxicity, which is the basis of immune surveillance for the containment of microbes and disease. The small intestine (SI) is the largest mucosal surface and is a primary site of pathogen entrance. Using two-photon laser scanning microscopy, we found that motility of antigen (Ag)-specific CD8αβ T cells in the SI is dynamic and varies with the environmental milieu.

Iron oxide labeling does not affect differentiation potential of human bone marrow mesenchymal stem cells exhibited by their differentiation into cardiac and neuronal cells.

Mesenchymal stem cells (MSCs) have shown promising outcomes in cardiac and neuronal diseases. Efficient and noninvasive tracking of MSCs is essential to harness their therapeutic potential. Iron oxide nanoparticles (IONPs) have emerged as effective means to label stem cells and visualize them using magnetic resonance imaging (MRI).

Self-Contained Statistical Analysis of Gene Sets.

Microarrays are a powerful tool for studying differential gene expression. However, lists of many differentially expressed genes are often generated, and unraveling meaningful biological processes from the lists can be challenging. For this reason, investigators have sought to quantify the statistical probability of compiled gene sets rather than individual genes.

Epigenetic response to environmental change: DNA methylation varies with invasion status.

Epigenetic mechanisms may be important for a native species' response to rapid environmental change. Red Imported Fire Ants ( Santschi, 1916) were recently introduced to areas occupied by the Eastern Fence Lizard ( Bosc & Daudin, 1801). Behavioral, morphological and physiological phenotypes of the Eastern Fence Lizard have changed following invasion, creating a natural biological system to investigate environmentally induced epigenetic changes.

Irresistible ants: exposure to novel toxic prey increases consumption over multiple temporal scales.

As species become increasingly exposed to novel challenges, it is critical to understand how evolutionary (i.e., generational) and plastic (i.

Modeling Honey Bee Populations.

Eusocial honey bee populations (Apis mellifera) employ an age stratification organization of egg, larvae, pupae, hive bees and foraging bees. Understanding the recent decline in honey bee colonies hinges on understanding the factors that impact each of these different age castes. We first perform an analysis of steady state bee populations given mortality rates within each bee caste and find that the honey bee colony is highly susceptible to hive and pupae mortality rates.

Harriet Hardy and the workers of Los Alamos: a campus-community historical investigation.

Harriet Hardy, protégé of Alice Hamilton, spent 1948 in the Health Division of Los Alamos Scientific Laboratory. The contemporary campaign for federal legislation to compensate nuclear workers brought to the fore living retirees in whose cases of occupational illness Hardy had a role in diagnosis or case management. A third case is documented in archival records.

Professional Nurse Coaching: Advances in National and Global Healthcare transformation.

Nurse coaches are responding to the mandate of Florence Nightingale (1820-1910)-the foundational philosopher of modern nursing-to advocate, identify, and focus on factors that promote health, healthy people, and healthy communities that are recognized today as environmental and social determinants of health.(1) (,) (2) The Institute of Medicine report(3) and other health initiatives suggest the need for increased education and leadership from nurses to address the healthcare needs of our nation and world. Nurse coaches are strategically pos-i tioned and equipped to implement health-promoting and evidence-based strategies with clients and support behavioral and lifestyle changes to enhance growth, overall health, and well-being.