Amino acid metabolism disorder and oxidative stress took part in EGCG alleviating Mn-caused ferroptosis via miR-9-5p/got1 axis.

Manganese (Mn) is not only an essential trace metal element in organisms, but also a water pollutant and damaging aquatic organisms. (-)-epigallocatechin gallate (EGCG), a natural tea polyphenol derived from green tea, may alleviate toxicity caused by environmental pollutants. Kidney is an important organ for excretion and expel toxins.

Deciphering the Effect of Postharvest 1-MCP Treatment Coupled with Low-Temperature Storage on the Physiological Activities and Edible Quality of Melon.

Fruits are an important source of a healthy diet due to their essential nutrients for daily intake. Melon is known as a significant fruit crop of the Cucurbitaceae family based on its various dietary benefits, but its shelf life needs to be maintained for long-term usage. 1-Methylcyclopropene (1-MCP) is a cyclopropene-derived synthetic plant growth regulator (PGR) that is used for significantly delaying the ripening process and maintaining the shelf life of climacteric fruits during storage.

Plants' Overexpression of the MYB Transcription Factor in the Face of Stress Hazards Enhances Salt and Cold Tolerance.

'Beta' ( × ) is a vine fruit tree of the genus Vitis which is a cross between American and riparian grapes. In the current situation of grape production in northern regions, cold, drought, and salinity are important bottlenecks restricting its development, while some grape rootstocks with excellent traits show the disadvantage of poor resilience. 'Beta' ( × ), one of the most extensively utilized rootstocks in viticulture, has demonstrated remarkable resilience to adverse conditions.

Genome-Wide Analysis of the Aspartate Aminotransferase Family in and the Role of in Response to Nitrogen Starvation.

Nitrogen (N) is the most important fertilizer for increasing crop production, as it is absorbed by various N transporters and metabolized by a series of enzymes. Aspartate aminotransferase (ASP) facilitates the conversion of Glu to Asp for N storage. Chinese cabbage is a typical leafy vegetable that requires a large amount of N for growth.

Inhibitory Effect and Mechanism of Dryocrassin ABBA Against .

Potato dry rot and wilt are the most important soil- and seed-borne diseases in potatoes. They cause high economic losses during potato growth and storage across the world. Previous observations have shown that dryocrassin ABBA can induce resistance in potatoes.

Integration of Multiomics Data Reveals Selection Characteristics of That Are Associated with Size Differentiation in Pigs.

Min pigs, a prominent local breed from Northeast China, have diverged into two distinct breeds, Ermin (EM) pigs and Hebao (HB) pigs, through prolonged natural and artificial selection. Although these two breeds exhibit distinct differences in body size, the genetic mechanisms underlying this variation remain poorly understood. In this study, we performed whole-genome resequencing and transcriptome analysis on EM and HB pigs to elucidate the genetic basis of body size variation in Min pigs through genome-wide selection signal analysis and the identification of differentially expressed genes (DEGs).

Combined Prokaryotic Transcriptomics and Proteomics Analysis of Clinical Isolates with Distinctive Cytotoxicity.

is a widely distributed opportunistic pathogenic bacterium that can infect livestock, wildlife, community animals, and humans, resulting in suppurative infection of tissue and organ mucosa, including pneumonia, liver abscessation, mastitis, metritis, endocarditis, and osteoarthritis. TP1804 and TP1808 were isolated from the uterine lavage fluid of cows with endometritis. This study analyzed the prokaryotic transcriptomics and proteomics of two strains of with similar growth curves but different cytotoxicity.

Glycyrrhizin Alleviates the Damage Caused by Zearalenone and Protects the Glandular Stomach of Chickens.

Zearalenone (ZEA) is a kind of mycotoxin that widely contaminates food and feed and poses a threat to poultry farming. As a natural extract, glycyrrhizin acid (GA) has antioxidant, antibacterial, and anti-inflammatory effects. Although studies have revealed the toxic effects of ZEA on the liver, the mechanism by which GA reduces ZEA's toxic protective glandular stomach remains unclear.

Numerical Study of Rice Grain Milling Uniformity in the Abrasive Milling Process.

The uniform removal of the bran layer significantly enhances the nutritional and economic benefits of rice. However, the influence of parameter conditions on the uniformity of milling in the abrasive milling process remains unclear. This is not conducive to improving the quality of rice milling.

Identification of DNA Methylation Differences in Pituitary Tissues of Sichuan White Geese Using Whole-Genome Bisulfite Sequencing (WGBS).

To explore the impact of epigenetic modifications on egg-laying traits in geese, we employed genome-wide bisulfite sequencing (WGBS) to analyze DNA methylation patterns in pituitary tissues of high-(HYP) and low-yield (LYP) Sichuan White geese. We achieved high-quality sequencing data (mean 19.09 Gb raw reads, 15.

Can Digital Human Capital Promote Farmers' Willingness to Engage in Green Production? Exploring the Role of Online Learning and Social Networks.

The development of rural digitalization has become a key driving force for promoting green agricultural production. However, in practical operations, due to the insufficient digital skills and lack of necessary digital human capital among farmers, they struggle to distinguish between green production methods and traditional practices, which in turn reduces their willingness to adopt green production. This study employed empirical research methods to collect data from 854 farmers in China's largest grain-producing region and used the Probit model to analyze the impact of digital human capital on farmers' willingness to engage in green production.

Comparative Transcriptomic Analysis Provides Insight into Spatiotemporal Expression Patterns of Pivotal Genes During Critical Growth Stages in Min Pig Breed.

The growth and development of animals are dynamic processes characterized by fluctuations. Min pigs, a local breed renowned for their superior meat quality, present an intriguing yet poorly understood relationship between this quality and their growth and development patterns. To elucidate this relationship, we employed a multi-faceted approach that included comparative transcriptomics, quantitative real-time PCR (qRT-PCR), selection pressure analysis of key genes, and three-dimensional protein structure simulations.

TNF/TNFR1 Signaling Mediates DEHP-Induced Hepatocyte Pyroptosis via the GSDMD-mtROS Axis.

Di(2-ethylhexyl) phthalate (DEHP), which is widely used in agricultural plastics, accumulates in humans and animals through the food chain over time, resulting in liver toxicity. Recent studies have reported that pyroptosis and mitochondrial damage are closely related to a variety of liver diseases, but the specific mechanism is still unclear. To address this issue, in vitro and in vivo hepatotoxicity models were established.

A new therapy to alleviate the inflammatory injury of piglet intestine caused by short-distance transportation--music.

The purpose of this study was to explore whether music can reduce stress in animals by regulating the activity of the hypothalamus pituitary adrenal cortex (HPA) axis and reducing the concentration of cortisol. The control group was not played with any music or mechanical noise, the music group played music before and during transport, and the noise group played noise before and during transport as a positive control. The results showed that after two-hours of transportation, the concentrations of adrenocorticotropic hormone and cortisol in the music group were lower than that in the control and the noise groups, while the cortisol level in the noise group was higher than that in the music and the control groups.

Metabolomics and Transcriptomics Reveal the Role of the Terpene Biosynthetic Pathway in the Mechanism of Insect Resistance in .

Terpenes are a large variety of natural organic compounds that can enhance the resistance of plants to phytophagous insects through induction. In this study, differential expression genes and metabolites of (AC) and (SH) were screened and analyzed by transcriptomics and metabolomics. The results show that terpene biosynthesis is one of the most crucial secondary metabolic pathways in plants.

Comparative Genomic Analysis of Two Monokaryons of Hei29.

is a valuable traditional Chinese fungus used as food and medicine. Hei29 is a strain derived from wild through systematic domestication and selection. It has been the primary variety in Northeast China for 30 years and offers the advantages of high yield, good commercial property, and stable traits.

New Dawn in the Treatment of Rheumatoid Arthritis: Advanced Insight into Polymer Hydrogel Research.

As a chronic systemic autoimmune disease, rheumatoid arthritis (RA) not only damages joints and other organs or systems throughout the body but also torments patients' physical and mental health for a long time, seriously affecting their quality of life. According to incomplete statistics at present, the global prevalence of RA is approximately 0.5-1%, and the number of patients is increasing year by year.

Effects of the stress hormone norepinephrine on the probiotic properties of : antibacterial colonization, anti-inflammation, and antioxidation.

Probiotics as antibiotic alternatives are unstable for use under stress in clinical applications. To explore the influence of catecholamine hormones on probiotic bacterial inhibition and antimicrobial activity, we tested the effects of norepinephrine (NE) on and in a mouse model. The results showed that in the presence of NE, 80% of strains showed increased growth rate and more than 80% of the strains indicated lower antimicrobial activity at 22 h.

Thymidylate Kinase-Targeted Antimicrobial Peptides via Phage Display: A Novel Strategy against Gram-Negative Bacteria.

The rise of antimicrobial resistance (AMR) in Gram-negative bacteria, including (), poses a major public health threat. This study aimed to address the limitations of existing antimicrobial peptides (AMPs) by designing hybrid peptides with enhanced targeting and antibacterial potency. Eight heptapeptide sequences were identified through phage display screening and hybridized with WP (WKKIWKPGIKKWIK), a peptide exhibiting weak antimicrobial activity against Gram-negative bacteria.

Design, Synthesis, and Biological Activity Evaluation of Novel Phenoxypyridine Derivatives Containing Acylthiourea Fragments as Protoporphyrinogen Oxidase Inhibitor Herbicides.

Protoporphyrinogen oxidase (PPO, EC 1.3.3.

A self-amplifying NO-HS loop mediates melatonin-induced CBF-responsive pathway and cold tolerance in watermelon.

Melatonin is a pivotal bioactive molecule that enhances plant cold stress tolerance, but the precise mechanisms remain enigmatic. Here, we have discovered that overexpressing melatonin biosynthetic gene ClCOMT1 or applying exogenous melatonin activates the C-repeat binding factor (CBF)-responsive pathway and enhances watermelon cold tolerance. This enhancement is accompanied by elevated levels of nitric oxide (NO) and hydrogen sulfide (HS), along with upregulation of nitrate reductase 1 (ClNR1) and L-cysteine desulfhydrase (ClLCD) genes involved in NO and HS generation respectively.

The influences of ARHGEF9 on myoblasts migration and differentiation.

Rho guanine nucleotide exchange factor 9 (ARHGEF9), as a protein that assists small GTPases, is widely present in various tissues. It has been reported to play an important role mainly in neurological diseases and gliomas. However, there have been no reports on its impact on skeletal muscle regeneration after injury.

One-Pot Synthesis of Hydrophobic Porphyrin Zirconium-Based MOFs for the Photoreduction of CO to Formate.

Using zirconium tetrachloride as a metal source and tetra(4-carboxyphenyl)porphyrin as a ligand and by in situ introducing octadecylphosphonic acid (OPA), three hydrophobic porphyrin zirconium metal-organic frameworks (MOFs) with different structural topologies were constructed, where these MOFs are labeled as OPA@PCN-222, OPA@PCN-223, and OPA@PCN-224. Compared with the original Zr-MOFs without the modification of OPA, the modified porphyrin Zr-MOFs show excellent hydrophobic properties and can maintain excellent stability in a long-term humidity environment. Meanwhile, OPA@PCN-222, OPA@PCN-223, and OPA@PCN-224 exhibit wide absorption of visible light and steadfast and expeditious photocurrent response by leveraging the properties of porphyrin ligands.

Discovery of (+)-Methyl Nonactate as a Potential Fungicide against Gummy Stem Blight.

Gummy stem blight (GSB), which is caused by , threatens pumpkin yields and agriculture. Effective, safe antifungal agents are urgently needed. In this study, fermentation broth supernatant of sp.

Optimization of extraction and enrichment process of cannabidiol from industrial hemp and evaluation of its bioactivity.

Introduction: The L., a perennial dioecious herb renowned for its industrial applications, serves as the source of hemp. Cannabidiol (CBD), a non-psychotropic compound derived from industrial hemp, has garnered considerable interest due to its promising therapeutic potential.