Biological function identification of phage holin Hol-4086 and treatment of infection.

The treatment of infections caused by drug-resistant has become increasingly difficult. In this study, the complete genome of phage 4086-1 against was sequenced and shown to be 17,960 bp in size, with a GC content of 29.14%.

Genome-wide analysis of SUMO conjugation pathway members in broccoli and the involvement of BoSIZ1 in response to ABA.

The small ubiquitin-like protein modifier (SUMO) is a conserved protein that modifies target proteins by attaching to them, changing their functions, localizations, and interactions. However, there is limited knowledge regarding the process of SUMOylation in broccoli (Brassica oleracea var. italica), a highly nutritious vegetable that is widely consumed.

Met-Exo attenuates pyroptosis in miniature pig liver IRI by improving mitochondrial quality control.

Metformin(Met) and adipose-derived stem cell exosomes(ADSCs-Exo) both demonstrate therapeutic effects on mitochondrial dysfunction and pyroptosis. There is also a phenomenon of mutual promotion between these two pathological states. The synergistic effect of metformin-loaded exosomes (Met-Exo) via electroporation in a miniature pig liver ischemia-reperfusion injury (IRI) model remains unexplored.

Unique isophorone-based NIR fluorescent probe with a high fluorescence quantum yield and larger stokes shift for Al and Zn level simultaneous monitoring in biological system.

Given the profound implications of Al and Zn in the ecosystem and organism, there is a compelling demand for a robust method to simultaneously detect these ions. The novel near-infrared (NIR) fluorescent probe DDLH based on isophorone, offers efficient in vivo monitoring capabilities for Al/Zn. Simultaneously, DDLH displayed excellent selectivity, anti-interference, a low detection limit and rapidity responsiveness to Al/Zn, coupled with a high fluorescence quantum yield and substantial Stokes shift (220 nm).

A programmable multichannel chemiluminescence immunoassay sensor for automatic quantification of the total amount of fumonisin B, B and B.

A programmable multichannel chemiluminescence immunoassay sensor was developed based on the biotin-streptavidin system for the automatic quantification of the total amount of fumonisin B, B and B in maize samples. First, the key parameters, such as the reaction time and reagent dosage were optimized to improve the sensitivity. All reagents were subsequently added to the corresponding wells of the reagent strips, which were further sealed to create a total fumonisin quantification kit.

Carbon Quantum Dots from Fallen Leaves of Lonicera caerulea L.: An Innovative Plant Growth Promoter and Fruit Quality Enhancer.

With increasing environmental pollution and resource wastage, utilizing waste for high-value applications has become crucial. This study explores the preparation of carbon dots (CDs) from blue honeysuckle leaves and their potential in enhancing plant photosynthesis. CDs derived from these leaves have a particle size of ∼2.

Effects of DA-6 and MC on the growth, physiology, and yield characteristics of soybean.

Background: As a grain and oil crop, soybean presents a much lower yield than other staple crops. However, crop yields can be improved by applying modern agricultural technology, such as diethylaminoethyl hexanoate (DA-6) and mepiquat chloride (MC), which are important plant-growth regulators that substantially affect crop growth and yield.

Methods: This study examined the effects of DA-6 (30, 60, or 90 mg L) and MC (100, 200, or 400 mg L) on soybean growth, development, root structure, photosynthetic physiology, osmotic regulation, and yield via field and pot experiments.

Stability and molecular interactions in cold-induced hazelnut protein-chlorogenic acid gel.

Background: Phenols have been demonstrated to enhance protein gelation but their stability is often compromised in heat-induced gels. This has prompted the development of cold-induced gels. The objective of this study was to enhance the gel properties of hazelnut isolate protein (HPI) through the incorporation of transglutaminase (TGase), glucono-δ-lactone (GDL), and chlorogenic acid (CA).

Physicochemical properties and texture of polymerized whey protein-auric polysaccharide and its incorporation into goat milk yogurt.

Polymerized whey protein (PWP) and Auric Polysaccharide (AP) are crucial functional biopolymers. However, their application in goat milk yogurt has not been explored. This study investigated the structural alterations within PWP-AP gels with the AP concentration varied from 0 to 1.

Effect of different natural antioxidants on the quality promotion of pork chip snacks during storage as revealed by lipid profiles and volatile flavor compounds.

This study primarily investigated the effects of different natural antioxidants (ascorbic acid, rosemary extract, PostbioYDFF-3®, and NatuProtec®) on changes in the lipid profiles and volatile flavors of pork chip snacks (PCS) during storage via lipidomic techniques and SPME-GC-MS. Compared with the control, the PCS containing different natural antioxidants exhibited obvious reductions in TBARS, peroxide, and acid values after 90-day storage (P < 0.05).

Epidemiology, genetic diversity and evolution of pigeon circovirus.

With the global prevalence of pigeon racing, pigeon infections with Pigeon circovirus (PiCV) have been reported across the world. PiCV-infected pigeons are susceptible to subsequent infections and exhibit a range of symptoms including weight loss, lethargy, anorexia, respiratory distress and diarrhea, which exerts a huge impact on the pigeon industry. However, there is currently a lack of epidemiological data on PiCV infection in pigeons.

Quercetin attenuates DEHP-induced pyroptosis and programmed necrosis in chicken duodenum through regulation of the TLR4/MyD88/NF-κB pathway.

Di(2-ethylhexyl) phthalate (DEHP) is not only popularly used as a plasticizer, but also ubiquitous in environment, causes an important risk to the lives and well-being of poultry. Quercetin (QUE) is a natural flavonoid with antioxidant, anti-inflammatory, anticancer and immunoregulation. Nevertheless, it's still unclear possibly DEHP causes duodenal pyroptosis and programmed necrosis in broiler chickens or perhaps QUE has a mitigating impact in this mechanism.

Ultrasound-assisted oligochitosan/casein complexes stabilized Pickering emulsion: Characterization, stability and its application for lutein delivery.

Lutein is a natural pigment with various beneficial biological activities, but its poor water solubility, chemical instability, and low bioavailability limit its application in food processing. In this study, modified casein (CAS-OCS NPs)-based Pickering emulsions were constructed under the combined effect of TGase-type glycation and ultrasound treatment as delivery systems for lutein fortification. Pickering emulsions based on CAS-OCS NPs enhanced the encapsulation efficiency of lutein (87.

Remediation strategies of biochar and microbial inoculum for PAHs-contaminated soil: Quorum sensing-mediated PAHs degradation and element cycling.

The pyrolysis of straw for biochar production offers significant advantages in resource recycling and soil improvement. However, the concurrent effects of biochar on contaminant degradation and biogeochemical cycles in polycyclic aromatic hydrocarbons (PAHs)-contaminated soil remain unclear. This study aims to integrate the roles of functional microorganisms, PAHs degradation pathways, and soil element cycles to elucidate the remediation characteristics of PAHs-contaminated soil.

Copper is essential for cyclin B1-mediated CDK1 activation.

Cyclin-dependent kinase 1 (CDK1) is the pivotal kinase responsible for initiating cell division. Its activation is dependent on binding to regulatory cyclins, such as CCNB1. Our research demonstrates that copper binding to both CDK1 and CCNB1 is essential for activating CDK1 in cells.

Protective effects of dietary supplementation of Bacillus Subtilis MZ18 against the reproductive toxicity of zearalenone in pregnant rats.

The microbial detoxification method demonstrates significant potential for detoxifying feed contaminated with mycotoxins, but the application of degrading bacteria in mammals was rarely investigated. In this study, the effects of dietary zearalenone on the growth performance, reproductive performance, reproductive organs, hormone levels of rats and the toxicity alleviation of Bacillus subtilis MZ18 were studied. From gestation day 0-20, pregnant SD rats received either a normal diet or a diet supplemented with zearalenone at a dose of 20 mg/kg⋅BW, and with or without supplementation of Bacillus subtilis MZ18 culture.

Plaque erosion risk and JAK2 V617F variant.

Background And Aims: Clonal haematopoiesis of indeterminate potential (CHIP) can increase the risk of myocardial infarction (MI). Among various CHIP mutations, JAK2 V617F substantially elevated this risk. However, the specific associations between JAK2 V617F and two mechanisms of MI, plaque erosion and plaque rupture, remain unclear.

The Function of PpKCS6 in Regulating Cuticular Wax Synthesis and Drought Resistance of Kentucky Bluegrass.

Drought stress significantly limits plant growth and crop productivity. Cuticular wax minimizes plant water loss and contributes to drought resistance. Kentucky bluegrass (Poa pratensis L.

Identification of the small auxin up-regulated RNA in tomato and investigation of SlSAUR50 as a positive regulator of tomato resistance to Botrytis cinerea.

The indole-3-acetic acid (IAA) signaling pathway plays a critical role in plant growth, development, and stress responses. Botrytis cinerea is a major biotic stress factor affecting tomato production. However, the mechanism by which small auxin up-regulated RNA (SAUR) genes participate in the response of tomato plants to B.

Enterococcus hailinensis sp. nov., Isolated from Traditional Chinese Pickle.

Two enterococcal isolates, designated as strains N48-2 and N48-9.1, were isolated from traditional Chinese pickle ('Suan cai') and characterized using a polyphasic approach. Phylogenies based on 16S rRNA gene sequences and whole genome sequences indicated that strains N48-2 and N48-9.

The transcription factor ClWRKY61 interacts with ClLEA55 to enhance salt tolerance in watermelon.

High salinity can severely inhibit the growth and development of watermelon ( L.). WRKY proteins are believed to mediate the adaptation of plants to abiotic stresses.

Molecular identification of a thioredoxin peroxidase in Babesia gibsoni with potential against oxidative stress.

Babesia gibsoni is the infectious agent of canine babesiosis, a vector-borne infection that poses a global threat to the canine health. As B. gibsoni is an erythrocytic intracellular parasite, the completion of its genome and transcriptome sequencing and analysis facilitates the elucidation of the mechanism of B.

Widely targeted metabolomics to analyze the effect of polyvinyl alcohol/pullulan/ZnO-Nps composite film on postharvest storage of Allium mongolicum regel.

In this study, a composite film (CF) made of polyvinyl alcohol, pullulan, and ZnO nanoparticles was prepared by solution casting. The CF were applied to the postharvest preservation of Allium mongolicum Regel. Widely targeted metabolomics analysis of A.

Co-exposure to ammonia and lipopolysaccharide-induced impaired energy metabolism via the miR-1599/HK2 axis and triggered autophagy, ER stress, and apoptosis in chicken cardiomyocytes.

Ammonia (NH) and lipopolysaccharide (LPS), common pollutants in poultry farming environments, pose significant health risks by disrupting cellular processes. Although previous studies have demonstrated the individual effect of NH or LPS on human and animal health, the mechanisms underlying their combined impact on chicken heart tissue remain poorly understood. In this study, we established a chicken cardiotoxicity model to investigate the effects of NH and/or LPS exposure on energy metabolism, autophagy, endoplasmic reticulum (ER) stress, and apoptosis in cardiomyocytes.

Clarifying the structure and characteristics of different varieties potatoes starch.

Through analyzing the physicochemical and structural characteristics of ten varieties potatoes, this study aimed to clarify how different amylose contents affected quality. The results showed that the amylose content ranged from 10.49 % to 20.