[Expression of vascular endothelial growth factor in different breast tissues and clinical significance thereof].

Objective: To investigate the differences in the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) count in breast benign affection, breast atypical hyperplasia, and breast carcinoma in situ and to clarify the association of VEGF expression and MVD with the clinicopathological features of these diseases.

Methods: Immunohistochemistry (SP-method) was used to examine the expression of VEGF and MVD count in 100 samples of breast benign affection (including 35 cases of breast fibroid tumor, 35 cases of breast cystic hyperplasia, and 30 cases of intraductal papilloma), and 15 samples of breast atypical hyperplasia, and 25 samples of breast carcinoma in situ, obtained during operation.

Results: The positive rate of VEGF of the breast carcinoma in situ group was 56% , significantly higher than hose of the breast benign affection and breast atypical hyperplasia groups (22% and 33% respectively, P < 0.

[Study on effect of demethoxycurcumin in Curcuma long on stability of curcumin].

Objective: To investigate effect of demethoxycurcumin on stability of curcumin.

Methods: To add the demethoxycurcumin to pure curcumin, the change of curcumin was determined by HPLC and the dynamics of curcumin degradation was investigated.

Result: The stability both obtained from alcohol and demethoxycurcumin improved the stabilization of curcumin, the demi-period of curcumin prolonged with the addition of demethoxycurcumin.

Outcomes for a large series of radical cystectomies for bladder cancer.

Aims: We present the characteristics and outcomes of a large Chinese series of patients treated with radical cystectomy and pelvic lymphadenectomy for invasive cancer of the bladder. Our aim is to determine the significant independent prognostic factors that determine this outcome.

Methods: The records of 356 patients with invasive bladder cancer, operated at three Chinese medical institutes between 1995 and 2004, were reviewed.

[Association of genetic polymorphism of XPD with chromosomal damage in workers exposed to radiation].

Objective: To explore association genetic polymorphism of XPD with chromosomal damage in workers exposed to radiation.

Methods: 182 workers exposed to radiation for at least one year with chromosomal damage were selected as cases based on a general health examination for all workers exposed to radiation in Tangshan city. The control group without chromosomal damage was matched to case by age (within 5 years), sex, work unit, type of exposed to radiation, cumulate serve length (within 1 year) according to 1:1.

[Effect of brain injury on expression of bone morphogenetic protein 2 in fracture healing process].

Objective: To investigate the changes in the expression level of bone morphogenetic protein 2 (BMP-2) in the bone callus of rats with femoral fracture and brain injury to explore the effect of the brain injury on the fracture healing and to explore the related mechanism.

Methods: Thirty-two 12-week-old SD rats weighing 368 +/- 25 g were randomly divided into 4 groups of 8 rats in each. The rats in Group A had a femoral fracture and a brain injury for 1 week; the rats in Group B had a femoral fracture but without brain injury for 1 week; the rats in Group C had a fracture and a brain injury for 2 weeks; and the rats in Group D had a fracture but without brain injury for 2 weeks.

[The affection and significance of NO on the expression of P-selectin in renal injury following hind limb ischemia/reperfusion in rats].

Aim: To probe into the affection and significance of NO on the expression of P-selectin in renal injury following hind limb ischemia/reperfusion in rats.

Methods: In accordance with the conventional approaches of our department, the model rats were prepared after they were made to undergo 4 hours or ischemia followed by 4 hours of reperfusion of hind limbs. The Wistar rats were divided into four groups randomly: Control group, LI/R group, L-Arg group and L-NAME group.

[Detection of Epstein-Barr virus in lung carcinoma tissue by in situ hybridization].

Objectives: To examine the Epstein-Barr virus (EBV) in primary lung carcinoma tissue, and to investigate the relationship between EBV infection and tumorigenesis of lung cancer.

Methods: Formalin-fixed and paraffin-embedded lung tissue specimens from surgically resected lung carcinoma tissues of 108 cases treated in Tanshan area from 2001 to 2006, which were confirmed further by histopathological examination after hematoxylin-eosin (HE) staining, were used to observe the EBV encoded RNA-1 (EBER1) using in situ hybridization (ISH).

Results: EBER1 was detected in 36 of the 108 primary lung carcinoma cases, and in 1 of the 22 normal lung tissues.

[Effect of phosphorylated c-Jun expression on COX-2 expression in the substantia nigra of MPTP mouse model of subacute Parkinson disease].

Objective: To investigate the effect of phophorylated c-Jun (p-c-Jun) expression on the expression of COX-2 in the substantia nigra (SN) of the MPTP mouse model of subacute Parkinson disease (PD) and explore the possible mechanism of the dopaminergic (DA) neuron death in PD.

Methods: C57BL/6N mice were treated with MPTP to establish subacute PD model. The changes of TH-, COX-2- and p-c-Jun-positive cells, and the expression levels of TH, COX-2 and p-c-Jun in the SN in the midbrain were observed with inmmunohistochemistry and Western blotting before and after administration of SP600125, a specific JNK inhibitor.

[Experimental study on umbilical vascular compliance and expression of antigen after removing endothelial cell].

Objective: To evaluate which is better method zymogen or low temperature frozen in removing vascular endothelial cell so as to lay a foundation for creating a kind of brace which is not to be rejected and the same as own blood vessel.

Methods: Fresh and not damaged umbilical blood vessel was collected from natural labour women, human umbilical blood vessel was remove carefully from normal foetus, then was put into disinfectant at 37 C for 24 hours. They were divided into 3 groups:normal group (NG), zymogen group (ZG) and low temperature frozen group (LG).

[Effect of MK801 on apoptosis in the development of brain injury after hind limbs ischemia/reperfusion in rats].

Aim: To evaluate development of brain injury after hind limbs ischemia/reperfusion (LI/R) in rats, and the effect of MK801 on the brain injury following LI/R.

Methods: The limbs ischemia/reperfusion model was established in rats. The MDA contents were evaluated in each group, apoptotic cells were detected with TUNEL, the expression of apoptosis-associated protein, such as bcl-2, cytoC and caspase-3 were determined with immunohistochemistry and Western-blot.

[Effect of oncolyn on activities of ATPase and neurotrophic factors expression in aging mice].

Objective: To study the changes of ATPase activety and neurotrophic factor expression of brain in aging mice and the Oncolyn's neutralization effect.

Methods: Kunming mice were randomly divided into control group, aging group and Oncolyn group. The aging models were made by subcutaneously injecting D-galactose at neck skin.

[Analysis of the risk factors of upper urinary tract dilatation due to spinal cord injury].

Objective: To evaluate the urodynamic risk factors of upper urinary tract dilatation (UUTD) secondary to spinal cord injury (SCI).

Methods: Ninety-six SCI patients of Tangshan earthquake were divided into 2 groups by ultrasonography: 16 SCI patients (group A) with UUTD and 80 SCI patients (group B) without UUTD received urodynamic test. Responses were evaluated using single and multiple analysis after examination.

[Effect of human silicotic alveolar macrophages on the expression of the collagen type I in human embryonic lung fibroblasts].

Objective: To study the effect of the cultured supernatant of human silicotic alveolar macrophages (AM) on the expression of the collagen type I in human embryonic lung fibroblasts.

Methods: Human alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 18 h. Then the cultured supernatant were used to culture human embryonic lung fibroblasts for 6 h, 12 h, 18 h, 24 h, 36 h, 48 h, 72 h.

[Relationship between gene polymorphism of transforming growth factor-beta and pneumoconiosis].

Objective: To explore the association between genetic polymorphisms of TGF-beta (TGF-beta) and susceptibility to pneumoconiosis.

Methods: One hundred and seventeen patients with pneumoconiosis were selected as case. The control group was workers exposed to dust but without pneumoconiosis who had the same sex, nationality, and workshop or work site as case.

[The injury of metronidazole on morphology of Giardia lamblia in vitro].

Trophozoites of Giardia lamblia were axenically cultivated with modified TYI-S-33 medium contained 500 microg/ml metronidazole (12h LC50). The morphology of drug-treated trophozoites was observed with light and electron micro-scopes at 2, 4, 8, 12 h respectively. The light microscopy revealed that the trophozoites treated with MTZ showed swollen, detached from the wall of the culture tube, and were with vacuoles in the cytoplasm.

[Expression of bcl-2 gene in EBV-transformed human gastric epithelial cell line GES-1].

Objective: To detect bcl-2 gene expression in Epstein-Barr virus (EBV)-infected human gastric epithelial cell line GES-1 for understanding the role of bcl-2 gene in the carcinogenesis of EBV-associated gastric carcinoma.

Methods: Akata 1061 cells producing recombined EBV carrying neomycin resistance gene (NEOr) was used to mediate the EBV infection of human gastric epithelial cell line GES-1 via close contact, with the empty plasmid pcDNA3-transfected GES-1 cells via lipofectamine method as a control. The EBV-infected and pcDNA3-transfected cells were cloned by limited dilution and the positive clones selected with G418.

[Effect of AcSDKP on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts stimulated by PDGF].

Aim: To investigate whether AcSDKP can inhibit proliferation and collagen synthesis in cultured rat cardiac fibroblasts mediated by PDGF.

Methods: Neonatal rat cardiac fibroblasts were isolated. The cell proliferation was observed by 3H-proline incorporation assay.

[Role of AcSDKP on collagen synthesis and degradation in cultured rat cardiac fibroblast].

Objective: To investigate the role of AcSDKP on collagen synthesis and degradation in cultured rat cardiac fibroblasts.

Methods: Neonatal rat cardiac fibroblasts were isolated and stimulated by PDGF. The cell proliferation was observed by (3)H-TdR incorporation assay.

[A study of chronic hepatitis B infection superinfected with hepatitis E infection].

Objectives: To compare the influence of hepatitis E virus (HEV) infection on the replication of hepatitis B virus (HBV) by observing clinical features, the relationship between the number of HBV DNA copies in serum, the degree of hepatic function impairments and prognosis of chronic hepatitis B patients with hepatitis E superinfection.

Methods: One hundred and fifteen chronic hepatitis B (CHB) patients and 115 CHB patients with hepatitis E (HE) superinfection were studied and compared. 74 liver tissue biopsy samples of the CHB and 51 of the CHB-HE sufferers were obtained.

[Expression NOS in acute lung injury following limb ischemia/reperfusion and its significance in rats].

Aim: To investigate the expression and role of inducible NOS (iNOS) and endothelial NOS (eNOS) in acute lung injury following limb ischemia/reperfusion (4h/4h).

Methods: Wistar rats were randomized into four groups: control group, ischemia/reperfusion (I/R) group, L-Arginine (L-Arg) pretreatment group, Aminoguanidine (AG) pretreatment group. The lung tissue of each group was subjected to assay of content of MDA, MPO, W/D and NO2-/NO3-.

[Relationship between genetic polymorphism of interleukin-6 and pneumoconiosis].

Objective: To explore the relationship between interleukin-6 (IL-6) (-634C/G) genetic polymorphisms and the pneumoconiosis.

Methods: A total of 104 male stage I pneumoconiosis patients diagnosed by the Pneumoconiosis Diagnosis Expert Panel according to the Chinese National Diagnosis Criteria of Pneumoconiosis (GBZ 70 - 2002) were selected. The pneumoconiosis comprised 66 silicosis and 38 coal worker' pneumoconiosis (CWP).

[Relationship between polymorphism of interleukin-1 and pneumoconiosis susceptibility].

Objective: To explore the relationship between the polymorphism of Interleukin-1 and the pneumoconiosis susceptibility.

Methods: Eighty patients with silicosis and 45 with coal workers' pneumoconiosis (CWP) were selected while 125 male workers, Han nationality in the same workplace as the patients were selected as the controls. Between the patients and the control, the differences of age and cumulative length of service were less than five years and two years, respectively.

[Human parasitology teaching in the 21st century].

Multimedia techniques were applied in parasitological teaching and experimental practices. In order to strengthen the practical ability of the undergraduate students, reform was conducted including a prioritization of the teaching content, use of series micro-slides and video show, etc.