Effects of calcitonin injected into various brain areas on pain threshold and Ca2+ in rats.

Aim: To study the effects of calcitonin (Cal) injected into different brain areas on pain threshold.

Methods: The analgesic effects of Cal were investigated in rats by the tail-flick test.

Results: Cal injected into lateral cerebral ventricle (LCV) or periaqueductal gray (PAG) increased obviously the pain threshold to 49 +/- 22% or to 68 +/- 12% (P < 0.

Radioimmunoscintigraphy of gastric adenocarcinomas with 99mTc-chimeric ccM4 antibody.

Fourteen patients with gastric lesions were studied for radioimmunoscintigraphy using the 99mTc-ccM4 chimeric antibody reactive with the human tumor-associated TAG72 antigen. These include 10 gastric adenocarcinomas, 3 gastric ulcers and 1 gastric lymphoma. Each patient received an infusion of 30-40 mCi 99mTc-ccM4 antibody.

[Antimicrobial properties of Flos Lonicerae against oral pathogens].

The antimicrobial effect of Flos Lonicerae on oral pathogens was studied. The results showed that 73.9% of the tested pathogens were inhibited at a concentration below 6.

Inhibition of motility and invasion of human lung cancer cells by invasion inhibiting factor 2.

The motility and invasion of cancer cells are basic requirements for the establishment of distant metastases. In this study, we examined the effect of invasion inhibiting factor 2 (IIF2), a motility/invasion regulatory agent, on the motility, invasion, growth and basement membrane attachment of human lung cancer cells. IIF2 significantly reduced cell dissociation, colony scattering and invasion induced by the motogenic factor, HGF/SF.

Effects of hepatic stimulator substance, herbal medicine, selenium/vitamin E, and ciprofloxacin on cirrhosis in the rat.

Background & Aims: Cirrhosis is a potentially lethal condition for which there is no proven effective therapy. The aim of this study was to compare the effects of hepatic stimulator substance, traditional Chinese herbal medicine, selenium plus vitamin E, and ciprofloxacin treatment on biochemical and histological features of fibrosis in rats with carbon tetrachloride (CCl4)/ethanol-induced cirrhosis.

Methods: One hundred twenty adult Wistar rats were divided into six study groups (20 rats/group): healthy controls, CCl4/ethanol-injured rats left untreated, and CCl4/ethanol-injured rats treated for 4 weeks with either hepatic stimulator substance, traditional Chinese herbal medicine, a combination of selenium plus vitamin E, or ciprofloxacin.

Calcium channel blockade and anti-free-radical actions of panaxatriol saponins in cultured myocardiocytes.

Aim: To identify the calcium channel blockade and anti-free-radical actions of panaxatriol saponins Re, Rf, Rg1, Rg2, Rh1, and oleanolic acid saponin Ro.

Methods: On ventricular myocytes of Wistar rats, single channel activities of B, L, and T type calcium channels were recorded with the cell-attached configuration of patch-clamp technic, and free radical contents were measured with electron spin resenance method.

Results: Re, Rg1, Rg2, and Rh1 shortened the open times, prolonged the close times, and reduced the open-state probabilities of B, L, and T type calcium channels; Rf shortened the open time, prolonged the close time and reduced the open-state probability of L type calcium channel; Ro did not influence the activity of calcium channels (60 mumol.

[Isolation and structure elucidation of cirensenosides O and P from the leaves of Oplopanax elatus Nakai].

Two new triterpenoid saponins were isolated from the leaves of Oplopanax elatus Nakai. By measuring physical and chemical constants and spectral data, their structures were elucidated as 3-epi-oleanolic acid 28-O-alpha-L-rhamnopyranosyl (1-->4)-beta-D- glucopyranosyl (1-->6)-beta-D-glucopyranoside; 3-O-beta-D-glucopyranosyl betulinic acid 28-O-beta-D-glucopyranosyl (1-->6)-beta-D-glucopyranoside and named as cirensenosides O and P, respectively.

[The structure activity relationship of saikosaponins and glycyrrhizin derivatives for Na+, K(+)-ATPase inhibiting action].

The study on structure-activity relationship of saikosaponins and glycyrrhizin derivatives for Na+, K(+)-ATPase inhibiting action has been made in vitro. The results showed that the order of potency of inhibitory effect of saikosaponins on Na+, K(+)-ATPase is as follows: b1 > d > b2 > b4 > a > b3 > e > c. The chemical structure C23-OH, C16-OH and the C11, C13 conjugated double diene of saikosaponins are important for its inhibitory activity.

Harmful effects of MSG on function of hypothalamus-pituitary-target gland system.

It has been demonstrated that neonatal administration of monosodium glutamate (MSG) results in a clearly defined lesion of the arcuate nucleus (AN) of the hypothalamus. The present study shows that fat was accumulated in the abdomen of male rats treated with MSG; weights of the body, pituitary and testis were lower; beta-EP content in hypothalamus decreased while L.EnK content increased; serum LH, FSH, TSH, GH and TS levels all decreased in varying degrees while serum PRL level significantly increased.

[Primary lymphomas of bone: a clinicopathologic study of 22 cases].

Unlabelled: 22 cases of primary lymphoma of bone (PLB) were reported. Multiple bone involvement in two patients, singular bone in twenty patients. One case had regional lymph node involvement.

[Effects of selenium and vitamin E on arachidonic acid metabolism in experimental myocardial ischaemia].

Wistar rats were fed with grain produced in the areas prevalent of Keshan Disease, and supplemented by selenium (Se) and vitamin E (VE) for ten weeks, and an acute myocardial ischaemia animal model was established by ligation of left anterior descending coronary arteries. Results indicated activities of phospholipase A2 (PLA2), creatine kinase (CK) and lactate dehydrogenase (LDH), and levels of arachidonic acid (AA), thromboxane A2 (TXA2), leucotriene C4 (LTC4) and lipid peroxides (LPO) increased, but level of prostacyclin (PC) and activities of glutathione peroxidase (GSH-PO), copper-zinc superoxide dismutase (Cu-Zn-SOD) and catalase (CAT) decreased 48 hours after ligation of the coronary vessels in rats without supplement of Se and VE. Supplement of Se or VE in their feed could reverse the above effects and the best results could be got by supplement of both.

Postnatal development of lymphoid follicles in rat Peyer's patches, with special reference to increased follicle number.

The postnatal development of Peyer's patches was investigated using routine histological and immunohistochemical techniques, focusing especially on the formation and increase in number of lymphoid follicles. In addition, we studied the influence on the development of lymphoid follicles in Peyer's patches of a sublethal dose of whole-body X-irradiation at an earlier postnatal age. At 1 day after birth, surface-IgM (sIgM)-bearing cells (B lymphocytes) were scattered throughout the Peyer's patch.

Simultaneous detection by consensus multiplex PCR of high- and low-risk and other types of human papilloma virus in clinical samples.

A consensus multiplex PCR (CM-PCR) technique was developed to detect high-risk (HPV 16/18), low-risk (HPV 6/11), and over 40 other types of human papillomavirus (HPV), separately but simultaneously, by mixing three pairs of consensus primers in the same PCR mixture, for gene amplification. Simultaneous detection of three groups of HPV DNA provides valuable information for clinical practice and this procedure is simple and convenient for routine laboratory examinations. We detected HPV DNA sequences in plasmid HPV DNA and DNA extracted from tissues of condyloma acuminata and cervical carcinoma and from exfoliated cells of the lower genital tract of healthy Chinese women living in the People's Republic of China.

Mild hyperthermia can induce adaptation to cytogenetic damage caused by subsequent X irradiation.

Many low-level environmental agents are able to induce an increased resistance to subsequent mutagenic effects induced by ionizing radiation. In this paper, an induced cytogenetic adaptation to radiation in human lymphocytes was studied with mild hyperthermia as the adaptive treatment and compared with that induced by low-dose radiation. We found that this adaptation could be induced not only in PHA-stimulated human lymphocytes (at 14, 38 and 42 h after addition of PHA), but also in unstimulated G0-phase cells (before addition of PHA) by mild hyperthermia (41 degrees C for 1 h) as well as 50 mGy X rays.

[Study on the relationship between uterine bleeding with intrauterine device and viral infection].

Objective: To study the relationship between uterine bleeding with intrauterine device (IUD) and virus infection.

Methods: Virus isolation from endometrium samples both with IUD and without IUD was performed with rabbit kidney cell. The cell pathogen effect (CPE) directed the positive isolation.

[Different effect of L-glutamate microinjection into medial or lateral habenular nucleu on pain threshold].

The habenulae (Hb) are related to modulation of pain sensation, but the results from different laboratories are inconsistant. The aim of the present investigation is to clarify the discrepancy. L-Glutamic acid (L-Glu) was microinjected into medial (MHb) and lateral habenular nucleus (LHb) on light anesthetized or conscious rats, and pain threshold was then determined on the different models of animal.

A study of immunoactivity of retinal S-antigen in retinoblastoma.

Purposes: To study retinal S-antigen expression in human retinoblastoma and assess if there is a correlation between S-antigen immunoactivity and degree of retinoblastoma cell differentiations.

Methods: Ten cases of Chinese retinoblastoma parafin-embedded tissues were applied for this study. A strain of monoclonal antibody,MabA9C6, which defines an epitope in S-antigen retained in fixed-tissue sections, was used to study S-antigen expression in 10 cases of retinoblastomas.

Induction of a cytogenetic adaptive response in germ cells of irradiated mice with very low-dose rate of chronic gamma-irradiation and its biological influence on radiation-induced DNA or chromosomal damage and cell killing in their male offspring.

In earlier studies we have shown that either a single exposure or multiple exposures to a low dose of X-rays (0.05 Gy) induced a significant cytogenetic adaptive response in mouse germ cells. In this paper, a very low-dose rate (20 microGy/min) of chronic 60Co gamma-irradiation was used to pre-irradiate mice for 40 days.

[Influence of the 2nd and 3rd grade abstracts from scorpion venom of Buthus martensii Karsch on action potentials of fast response myocardiocytes].

Aim: To screen the active components of sodium channel blockade action from Buthus martensii Karsch (BMK) scorpion venom.

Methods: Myocardiocytes of mice were cultured. Action potentials of fast response myocardiocytes were recorded.

[Application of oesophagogastric double anastomosis in the treatment of esophageal caustic stricture].

Between 1982 and 1992, nine cases of oesophageal caustic stricture underwent oesophagogastric double anastomosis with success. There was no need to dissect and resect the strictured esophagus, and traumatizing organs adjacent to oesophgus was avoided. This method also eliminated the possibility of occurrence mediastinal abscess due to postoperative rebindling or infection by the esophageal segment.