Using retrograde island flap with dorsal interosseous artery to repair soft tiss ue defect of hands.

Since the report of retrograde island flap with radial artery by Lu and Wang in 1982 and retrograde island flap with ulnar artery by Li in 1984, the flaps from forearm has been commonly used clinically in recent years because of its good textrue and satisfactory therapeutic results. However, whether the function of hand will be impaired by cutting one of the main arteries which supply the hand still remains controversial. There has been an acute ischemia of hand reported in foreign literature.

[Corneal epithelium implantation under corneal flap after excimer laser in situ keratomileusis: a clinical pathological and cytological study and management].

Objective: To study the clinical pathological and cytological characteristics of corneal epithelial cells implanted under the cap of laser in situ keratomileusis and the method of management.

Methods: For 4 eyes with epithelial cell implantation, the corneal cap was torn from temporal side to nasal, and the implanted material was removed from the flap and the stromal base. After washing between the interface, the cap was repositioned.

[Expression of lipofect AMINE mediated human GM-CSF eukaryotic expressing vector in HFCL cells].

Objective: To construct an eukaryotic expressing vector-pIRES1neo/hGM-CSF and express it in human bone marrow stromal cell line HFCL.

Methods: Human granulocyte/macrophage colony-stimulating factor cDNA (hGM-CSF cDNA, 751 bp) was inserted into an effective eukaryotic expressing vector-pIRES1neo which contains the human cytomegalovirus (CMV) major immediate early promoter/enhancer and the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV). HFCL cells were transfected with the recombinant vector pIRES1neo/hGM-CSF by liposome-mediated gene transfer method.

[The relationship between laminin-receptor and nm23 protein expression and its correlation with interstitial microvascular density and tumor metastasis in breast carcinoma].

Objective: To study the relationship between 67,000 laminin, laminin-receptor (LN-R) and nm23 protein expression, interstitial microvascular density (MVD) and tumor metastasis in breast carcinomas.

Methods: The expression of laminin (LN), LN-R, FVIIIRAg and nm23 protein were detected in 73 cases of breast carcinoma with immunohistochemical technique and analyzed.

Results: A significant difference in LN expression was found in breast carcinoma according to their pathological grade.

G1 arrest and relative protein expressions in mouse thymocytes induced by whole body X-ray irradiation.

Objective: To investigate the molecular regulation of G1 arrest of mouse thymocytes induced by ionizing radiation.

Methods: Cell cycle was analyzed by flow cytometry (FCM) following staining of cells with propidium iodide. Fluorescent staining and flow cytometry analysis were employed for measurement of protein expression.

[Relation between Beta-2-glycoprotein I and hepatitis B virus surface antigen].

Objective: To clarify the binding character between Beta-2-glycoprotein I (Beta-2-GPI) and HBsAg.

Methods: Beta-2-GPI was purified from human plasma and labelled with biotin. Solid phase enzyme linked absorbance assay was used to investigate its binding with HBsAg.

[Effect on keratocyte-mediated collagen degradation by Pseudomonas aeruginosa].

Objective: To study the pathogenesis of cornea melting (ulceration) by pseudomona (P) aeruginosa for instruction of clinical treatment.

Methods: Type I collagen gels with or without suspended keratocytes were incubated for 24 hours under medium containing sterile P. aeruginosa culture broth.

[Comparative study of portal-systemic shunt rate measured by formula and radioactive method].

Objective: To establish a new simple and useful formula for the observation of portosystemic shunt.

Method: In the same animals and the same patients, portosystemic shunt rate was measured individually by the two different methods, i.e.

[Effect of losartan on arterial blood pressure and unit discharging of neurons in LHb and MHb of rat].

Aim And Methods: To investigate the effect of 2 mg/kg and 10 mg/kg losartan intraperitoneally (i.p) on arterial blood pressure (AP) and heart rate (HR) in rat and the involvement in the activity of habenulas neurons. Glass micropipette was used to record any changes of unit discharging of neurons in LHb and MHb before and after losartan was intraperitoneally injected.

[Pharmacokinetics of adriamycin by bronchial artery infusion for non-small cell bronchogenic carcinoma].

Objective: This pharmacokinetic study was conducted to determine the plasma concentration of adriamycin after bronchial artery infusion (BAI), and to compare it with the conventional chemotherapy.

Methods: Twenty-four healthy rabbits were divided into 4 groups with 6 animals in each. Comparison of the parameters was investigated in pharmacokinetics after ear-lobe venous injection of ADM (Group 1) and in combination with DDP or/and MMC (Groups 2, 3, 4).

[Dynamic changes of neurotrophic factors in different cerebral areas after full cerebral ischemia and reperfusion in old rats].

Objective: To explore the relationship between vascular dementia and nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF).

Methods: 42 male and female senile Wistar rats were randomized into control and experimental groups. The control group was subdivided into A, and C three groups and the experimental group into 6 subgroups, i.

[Expression of C-erbB-2 oncogene mRNA in salivary gland tumors].

Objective: To study relationship of C-erb-2 oncogene mRNA expression and histotype, tumorigenesis and biological behavior of salivary gland neoplasms.

Methods: Using 32P labeled oligonucleotide as probe, the dot blot technique was used to study the expression of C-erbB-2 oncogene mRNA in salivary gland neoplasm with normal salivary gland as control.

Results: With the expression in normal salivary gland as a standard, low C-erbB-2 mRNA expression was seen in adenolymphoma, basal cell adenoma.

[Cloning and biological regulating effects of human WAF1 gene].

Objective: To clone human WAF1 gene and construct WAF1-pcDNA3 recombinant plasmid, and to explore the biological regulating effects of WAF1 gene.

Methods: Human WAF1 gene was amplified and cloned into pcDNA3 vector by RT-PCR and DNA direct sequencing from Hela cells. By immunofluorescence and immunohistochemistry staining, the identify the expression of the inserted WAF1 gene and its biological regulating effects on Rb, cyclin D1 gene.

[Changes of Ca2+ channel behaviors in bronchial smooth muscle cells of asthma guinea-pig model].

Objective: To investigate the relationship between the change of calcium channel behavior in bronchial smooth muscle cells (BSMCs) and the airway hyperresponsiveness in guinea-pigs with asthma.

Methods: The changes of calcium channel dynamics in BSMCs of 4 asthma guinea-pig models (22 cells) and 5 normal guinea-pigs (25 cells), and the inner peak calcium current in BSMCs of 5 asthma guinea-pig models (25 cells) and 6 normal guinea-pigs (24 cells) were determined by patch-clamp techniques.

Results: (1) The open time of calcium channel in BSMCs of asthma group was (1.

[Analysis of Myc gene family in laryngeal cancer].

Objective: To study the abnormal amplification and role of Myc gene family in the development of laryngeal cancer.

Method: A PCR-PAGE-Laser scanning technique was applied.

Results: There was no Myc gene amplification in the normal laryngeal tissues.

[Antivirus effect of polysaccharides of brewer yeast in vitro].

The antivirus effect of polysaccharides of brewer yeast from yeast mud on 13 kinds of viruses including DNA and RNA virus along with their mechanisms were studied. The result showed that this effect was remarkable on the infections with poliovirus III, adenovirus III, ECHO6 virus, enterovirus 71, vesicular stomatitis virus, herpesvirus I, II, coxsackie A16 virus and coxsackie B3 virus. The polysaccharides of brewer yeast could also inhibit the development of cytopathic effect(CPE) and protect cultural cells from being infected with the above viruses.

Intercellular adhesion molecule-1 and accumulation of eosinophils in nasal polyp tissue.

Objective: To investigate the relationship between intercellular adhesion molecule-1 (ICAM-1) and the accumulation of eosinophils in nasal polyp tissue to better understand the mechanism of airway eosinophilic inflammation.

Methods: The expression of ICAM-1 and its natural ligand, lymphocyte function-associated antigen-1 (LFA-1), in normal nasal mucosa from 6 controls and in nasal polyp tissue from 19 patients with nasal polyposis were determined with immunohistochemistry. With dual immunohistochemistry and May-Griünwald Giemsa stain (MGG), the expression of LFA-1 and infiltrating eosinophils in nasal polyp tissue was observed.

[Fabrication of tissue engineered skin equivalent].

Objective: To fabricate artificial human skin with the tissue engineering methods.

Methods: The artificial epidermis and dermis were fabricated based on the successful achievements of culturing human keratinocytes(Kc) and fibroblasts (Fb) as well as fabrication of collagen lattice. It included: 1.

[Experimental study in rabbit on repair of radial bone defect by bioactive degradable polymer].

Objective: To investigate the availability of bone defect repair by degradable porous polycaprolactam (PCL) as the carrier of bone morphogenetic protein (BMP).

Methods: Three different kinds of bone substitutes, including decalcified bone matrix, PCL-BMP compounds and simple PCL, were implanted into the radial bone defects in 36 rabbits, and in the other 12 rabbits the bone defects were left untreated as control. X-ray examination, X-ray morphometry, histological and electron microscopic observation were performed at different time after operation.

[Treatment of simple obesity in children with photo-acupuncture].

Objective: To study the treatment of simple obesity in children and observe its effect.

Methods: Two hundred and two patients were treated with self-produced photo-acupuncture apparatus (101 cases) or ear-pressing (101 cases), and their effects were observed and compared.

Results: Effects of both methods were satisfactory.

[A new flavonoid glycoside from Allium cepa L. var agrogatum Don].

Aim: To investigate the chemical constituents from the bulb stems of the Chinese medicinal plant Allium cepa L. var. agrogatum Don.

[Study on tumor necrosis factor and pathogenesis of pregnancy induced hypertension].

Objective: To study the role of tumor necrosis factor (TNF-alpha) in the pathogenesis of pregnancy induced hypertension (PIH).

Methods: Radioimmunoassay was used to measure the levels of TNF-alpha in 25 severe PIH patients and 25 normal pregnant women. Umbilical vein endothelial cells were cultured with TNF-alpha (500 U/ml) or without TNF-alpha.

[Effect of trichosanthin of cell cycle and apoptosis of murine melanoma cells].

Objective: To study the inhibitory effect of purified trichosanthin component on the proliferation of malignant melanoma.

Methods: The effect of purified trichosanthin component on the DNA synthesis, cell cycle and cell apoptosis of murine melanoma cells were detected by flowcytometry when cultured in vitro.

Results: The significant G0/G1 phase arrest was revealed by the increase of cells in G0/G1 phase and decrease of cells in S phase.

[Expression of a human FL eukaryotic expressing plasmid mediated by lipofectamine in HFCL cells].

Aim: Bone marrow stromal cell line-HFCL were transfected with the recombinant eukaryotic expressing vector-pIRESlneo/hFL by using liposome-mediated gene transfer method and get a stable expression.

Methods: HFCL cells were transfected with the recombinant eukaryotic expressing vector-pIRESlneo/hFL by using liposome lipofectamine. Integration of hFL in the genome, transcription of hFL mRNA and expression of hFL protein in the transfected HFCL cells were assayed by Southern blot, Northern blot, Western blot and ELISA, the experiment of the human umbilical blood CD34+ cell multiplication.