A comprehensive review of advances in hepatocyte microencapsulation: selecting materials and preserving cell viability.

Liver failure represents a critical medical condition with a traditionally grim prognosis, where treatment options have been notably limited. Historically, liver transplantation has stood as the sole definitive cure, yet the stark disparity between the limited availability of liver donations and the high demand for such organs has significantly hampered its feasibility. This discrepancy has necessitated the exploration of hepatocyte transplantation as a temporary, supportive intervention.

Advancing kidney xenotransplantation with anesthesia and surgery - bridging preclinical and clinical frontiers challenges and prospects.

Xenotransplantation is emerging as a vital solution to the critical shortage of organs available for transplantation, significantly propelled by advancements in genetic engineering and the development of sophisticated immunosuppressive treatments. Specifically, the transplantation of kidneys from genetically engineered pigs into human patients has made significant progress, offering a potential clinical solution to the shortage of human kidney supply. Recent trials involving the transplantation of these modified porcine kidneys into deceased human bodies have underscored the practicality of this approach, advancing the field towards potential clinical applications.

[Discovery of Cervus nippon Temminck-derived sentrin/SUMO].

In order to identify unknown encoding cDNAs of Cervus nioppon Temminck (sika deer), we constructed a cDNA library of uterus from Jilin-Shuangyang Cervus nippon Temminck using PCR cDNA library kit. PCR products of the library were cloned into pGEM-Teasy vectors and the cDNAs were sequenced and analyzed by nucleotide homology comparison against GenBank Database using the BLAST network service. The results showed that the cDNA library contained cDNA fragments of different lengths and a full length encoding cDNA highly homologous to human sentrin-1/SUMO-1 (small ubiquitin-related modifier 1) was identified.

[High expression of human thrombopoietin mutant fusion protein in E. coli].

Objective: To obtain human thrombopoietin (Tpo)mutant cDNA encoding mature peptide N terminal 1 approximately 196 amino acids and investigate its expression in E. coli JM109.

Methods: Polymerase chain reaction and DNA recombination techniques were employed.

[Study of antisense oligodeoxynucleotide in reversing multidrug resistance and inducing apoptosis of tumor cells].

Objective: T o explore an approach to the reversal of multidrug resistance(MDR) of tumor cells.

Methods: Antisense oligodeoxynucleotide (mdr-1-AS PS-ODN) was used.

Results: A large amount of DNA fragments was found in mdr-1-AS PS-ODN treated K562/ADM cells and almost all the mdr-1+ K562/ADM cells underwent apoptosis.

Involvement of the Ca(2+)-protein kinase C and adenyilate cyclace signal pathways in the activation of thymocytes in response to whole-body irradiation with low dose X-rays.

Objective: To study the molecular mechanism of the stimulatory effect of low dose radiation (LDR) on T cell activation.

Methods: Thymocytes from Kunming mice exposed to whole-body irradiation (WBI) with different doses of X-rays were analyzed for the changes in signal molecules of the phospholipase C-phosphatidylinositol biphosphate (PLC-IP2) and G protein-adenylate cyclase (AC) pathways.

Results: It was found that [Ca2+]i increased in response to doses within 0.

[Intestinal bacteria metabolism of TSDP and characterization of metabolites in rats].

Objective: To study the rat intestinal bacteria metabolism of total saponins of Dioscorea pathaica (TSDP) in vitro, and characterize the metabolites in serum and urine of rats after oral administration of TSDP 900 mg.kg-1.

Method: TSDP metabolites were detected with thin-layer chromatography (TLC) and combination of electrospray ionization mass spectrometry (ESI-MS) and sequential tandem mass spectrometry (MSn).

[Comparative study on anti-hypercholesterolemia activity of diosgenin and total saponin of Dioscorea panthaica].

Objective: To compare the anti-hypercholesterolemic and cholesterol absorption inhibitory activities between total saponin of Dioscorea panthaica (TSDP) and diosgenin (Dio).

Method: TSDP and Dio were given ig or i.p.

[Effect of Rg2 on hemodynamics of hemorrhagic shock and its antioxidation in dogs].

Objective: To investigate the protective effect of Rg2 on hemodynamics of hemorrhagic shock and its antioxidant properties.

Method: Twenty mongrel dogs were randomly divided into Rg2 group, Shen Mai group and control group. The hemorrhagic shock model was built in all dogs by artery bleeding and mean arterial blood pressure was kept < 5.

[Role of P53, nm23 proteins and vascular endothelial growth factor in angiogenesis and metastasis of laryngeal cancer].

Objective: To elucidate the role of P53, nm23 proteins and vascular endothelial growth factor (VEGF) on angiogenesis and metastasis of laryngeal cancer.

Methods: Specimens of 42 laryngeal carcinomas were studied by immunohistochemical staining for P53 protein, nm23 protein, VEGF and CD34 antibody.

Results: Positive expressions of P53 protein, nm23 protein and VEGF were found in 47.

[Expression of matrix metalloproteinasa-2,9 in cholesteatoma and middle ear cancer].

Objective: To study various expressions and roles of MMP-2, 9 in cholesteatoma and middle ear cancer.

Methods: MMP-2 and MMP-9 were detected with immunohistochemical methods using monoclonal antibody in 36 cholesteatomas, 10 CAMS and 16 middle ear cancer.

Results: The stain in cholesteatoma was stronger than that in CAMS and NAMS.

[Effects of cadmium chloride on the malignant transformation of human embryo lung fibroblasts].

The malignant transformation of human embryo lung fibroblast (HELF) induced by CdCl2 was tested by 3H-TdR incorporation, chromosome analysis and flow cytometry. Chromosome aberration, the ratio of cells in different cell cycle phases and the quantities of DNA synthesis of different groups were determined. The results showed that the morphology of HELF changed in three levels of cadmium chloride (I 0.

Regulation effect of vascular endothelial growth factor on human fetal choroid vascularization.

Purpose: To investigate the spatial and temporal regulation effect of vascular endothelial growth factor (VEGF) on human fetal choroid vascularization.

Methods: The eyeballs of 54 human fetuses from the 9th week to the 40th week due to accidental abortion were studied by immunohistochemically staining for the expression of VEGF and proliferation cell nuclear antigen (PCNA).

Results: (1) The distribution of VEGF expression in the retinal pigment epithelium (RPE) decreased with the increase of age, the peak of which was between the 9th and 14th week.

[Determination of T lymphocytes and trace elements in spleen from rats infected with Toxoplasma gondii].

Objective: To determine the level of five trace elements(Fe2+, Cu2+, Zn2+, Ca2+, Mg2+) in the spleen and changes of T lymphocyte and its subtype variations in peripheral blood from the rats infected with Toxoplasma gondii.

Methods: Twenty rats were randomly and equally divided into two groups: control group and experiment group. Each rat in the experiment group received an i.

[Detection of DNA of Toxoplasma gondii in rat by using polymerase chain reaction].

Objective: To study the significance of DNA of Toxoplasma gondii in peripheral blood.

Methods: DNA of T. gondii in peripheral blood of 50 infected rats was detected by polymerase chain reaction.

[Analysis of trace elements in liver, spleen and brain of rats infected with Toxoplasma gondii].

Objective: To study the level of five trace elements(Fe2+, Cu2+, Zn2+, Ca2+, Mg2+) in the liver, spleen and brain of the rats infected with Toxoplasma gondii.

Methods: 20 rats were randomly and equally divided into two groups: normal group and experimental group. On the 64th day after injection of 1.

[Comparative effect of different media in in vitro cultivation of Trichomonas vaginalis].

Objective: To investigate the optimal condition for in vitro cultivation of Trichomonas vaginalis for obtaining a better harvest of T. vaginalis.

Methods: An isolate of T.

[Study of the relation between MMP2, MMP9 and nasopharyngeal carcinoma].

Objective: To evaluate the role of matrix metalloproteinase (MMP) in the invasion and metastasis of nasopharyngeal carcinoma (NPC).

Method: Twenty-six cases of NPC were studied by immunohistochmical method using monocolonal antibody to MMP2 and MMP9.

Result: It was showed that there were significantly correlation between MMP2 or MMP9 and the invasion and metastasis of NPC.

[Cell infiltration in nasal polyps and it's pathological significance].

Objective: To study the relation between inflammatory cells infiltration in nasal polyp tissues and it's pathogenesis.

Method: Nasal mucosa from 10 normal individual and 4 patients of nasal polyps were studied with immunohistochemical technique and histopathologic examination.

Result: The number of eosinophils, CD68 positive cells and monocytes was significantly higher (P < 0.

[Plasmid DNA expression in the muscle of rat tongue].

Objective: To develop rat tongue model for plasmid DNA expression in vivo.

Methods: The expression vectors which contained either human-muscle specific promoter or SV40 promoter directing the transcription of reporter gene (E. coli LacZ gene) were used.

[Studies on chemical constituents in the roots of Rabdosia japonica (Burm.f). Hara var. glaucocalyx (Maxin) Hara].

Objective: To isolate and elucidate the chemical constituents in the roots of Rabdosia japonica.

Method: The constituents were extracted by ether and isolated by chromatography on silica gel and ODS. The structures were determined by IR, H NMR, 13C NMR and MS spectra respectively.

[Pollen morphology of bee plants in Changbai Mountain area].

Objective: To develop and utilize the bee plants and bee products in Changbai Mountain area, the representative area of alpine plants in northeast China featuring abundant resources of bee plants.

Method: The pollen of bee plants was observed and studied by LM and SEM.

Result: The pollen is morphologically oblate, suboblate, spheroidal and prolate.

[Effects of glucocorticoids on cardiovascular response after microinjection of noradrenaline/neuropeptide Y into nucleus tractus solitarius and the underlying mechanism in rats].

The present study was designed to observe the effect of glucocorticoids in the NTS on cardiovascular regulation, and to explore the effect of glucocorticoids on cardiovascular responses to microinjection of NA/NPY into the NTS. A large dosage of Dex was found to evoke a decrease of blood pressure and heart rate, and an increase of serum NO concentration. A small dosage of Dex rapidly attenuated or even abolished the cardiovascular responses to NA/NPY microinjected into NTS, and this effect continued for a longer period.

[Involvement of habenula nucleus in the pathogenesis of stress induced hypertension and the underlying mechanisms].

Stress-induced hypertension (SIH) was performed by electric shock on hind paw in rats. Habenula(Hb) lesion decreased the degree of SIH. After stress, the Ang II concentration in blood plasma and in Hb were increased.

Long-term prognostic analysis of thymectomized patients with myasthenia gravis.

Objective: To study the factors affecting the long-term prognosis of patients with myasthenia gravis (MG) after thymectomy.

Methods: 170 MG patients who had undergone thymectomies were studied retrospectively. Among them, 124 patients received long-term follow-up for more than 40 months postoperatively.