[IFN or oxymatrine in combination with lamivudine in patients with lamivudine-resistant chronic hepatitis B].

Objective: To observe the therapeutic efficacy of IFN or oxymatrine in combination with lamivudine in patients with lamivudine-resistant chronic hepatitis B.

Methods: Forty patients ongoing treatment with lamivudine were randomized to three groups: group A, 14 patients with addition of IFN alpha-2b 3MU to ongoing lamivudine, daily, one month, followed by the same dose given every other day, five months; group B, 15 patients with addition of injectable oxymatrine 60 mg daily, three months, followed by oral oxymatrine every day, three months, and group C, 11 patients ongoing treatment with lamivudine alone. The HBV DNA level in serum, HBeAg seroconversion, and ALT level were detected at the end of the treatment.

[Clinical research on the effect of Oxymatrine on serum cholinesterase].

Background: To investigate the effect of Oxymatrine (OM) on serum cholinesterase (ChE) during the treatment of viral hepatitis and the relationship between the change of ChE and the change of albumin (ALB), prothrombin activity (PTA) and other liver function tests.

Methods: A total of 98 patients with viral hepatitis were divided into four groups. Group A consisted of 31 patients and were treated with OM intravenous infusion; Group B consisted of 30 patients, treated with OM orally; Group C consisted of 7 patients and were treated with OM intramuscular injection while Group D consisted of 30 patients, and were not treated with OM.

[A prospective study on short period antibiotic treatment of hepatic failure complicated with spontaneous bacterial peritonitis].

Background: To observe the effects of short-term antibiotic treatment in patients with hepatic failure and spontaneous bacterial peritonitis (SBP).

Methods: In this prospective study short-term antibiotic treatment was given to 67 cases diagnosed as hepatic failure with spontaneous bacterial peritonitis. Ceftriaxone 2 g, iv drip, q12h for 10 d or ofloxacin 0.

[Construction and expression of DNA-binding domain plasmid with hepatitis B virus e antigen in yeast double hybrid system].

Background: Using hepatitis B virus e antigen (HBeAg) gene to construct the DNA-binding domain vector, which can express HBeAg in yeast cell, and can be used in yeast double hybrid as "bait plasmid" to look for the gene from the cDNA library, which expresses the protein that can interact with HBeAg.

Methods: PCR was performed to amplify the HBeAg gene from a sera of hepatitis B patient. The product of the amplification was inserted into T-vector and was verified by sequencing.

[Association of T cell subsets with clinical status and hepatic pathology in children with chronic hepatitis B virus infection].

Background: To study characteristics of peripheral T cell subsets in 94 children with chronic hepatitis B and to elucidate its relationships with clinical status and hepatic pathology.

Methods: Peripheral T cell subsets were detected using flow cytometric analysis with specific monoclonal antibodies staining in 94 patients with HBV infection. The authors simultaneously detected their serum ALT, markers of HBV infection and examined liver biopsy material for pathological changes.

[Effect of Composite Yinchen Tablet on lymphocyte proliferation in mice].

Objective: To observe the effect of Composite Yinchen Tablet (CYT) on lymphocytes proliferation in mice.

Methods: By the method of 3H-TdR admixing, the effect of CYT on lymphocytes proliferation in mice were detected in vivo and in vitro.

Results: CYT in high, medium and low dose could significantly increase the cpm level of 3H-TdR.

[Proceedings of new drug delivery systems and their applications in the traditional Chinese drugs].

By referred to a lot of data, some new drug delivery systems(DDSs) including the Sustained and Controlled DDS, the Targeted DDS, the Transdermal DDS, the Bioadhensive DDS, the PowderJect DDS and the Self-Emulsifying DDS and their applications in TCD since 2000, will be summarized and some latest DDSs in the world including drug-eluting stents, gene therapy carrier system, biological chip, biomolecular motor-powered nanodevice and nanotrap will be also introduced in this paper. The objective of this paper is to introduce the new DDSs proceedings of and their applications in the Traditional Chinese Drugs(TCDs) and to provide some references for the pharmaceutics of TCD. For several recent years, the great success have been achieved in studying the new DDS application in the change of preparation of TCD by the investigators at home, but there is a large difference between at home and at board.

[Polymorphism of CXCR4 coding region of human immunodeficiency virus-1 in Chinese Han people].

Objective: To study the polymorphism of human immunodeficiency virus (HIV)-1 coreceptor CXCR4 in Chinese Han ethnic group for AIDS prevention and treatment.

Methods: Totally 48 individuals were enrolled into the study. CXCR4 (cDNA No-AF147204) was cloned by PCR amplification using 2 pairs of primers, then sequenced using sequencing primers.

[Relationship between cholinesterase, prothrombin activity and albumin and the pathology of the liver].

Background: To investigate the relationship between serum cholinesterase, albumin and plasma prothrombin activity and the pathology of the liver in patients with viral hepatitis.

Methods: Totally 135 patients diagnosed as chronic hepatitis, cirrhosis and/or severe hepatitis with needle biopsy of liver were enrolled in this study. Serum cholinesterase and albumin of the patients were detected with enzyme rate method; meanwhile, their plasma prothrombin activity was detected with turbidimetry test.