ING5 differentially regulates protein lysine acetylation and promotes p300 autoacetylation.

ING5 belongs to the Inhibitor of Growth (ING) candidate tumor suppressor family. Previously, we have shown that ING5 inhibits invasiveness of lung cancer cells by downregulating EMT-inducing genes. However, the underlying mechanisms remain unclear.

[Research of mHLA-G and the receptor expression with kidney rejection and CMV active infection following kidney transplantation].

To study the expression of membrane HLA-G (mHLA-G) and the receptor immunoglobulin-like transcript 2(ILT2) on lymphocyte and find their association with rejection and cytomegalovirus (CMV) infection after renal transplantation. A total of 88 cases of renal transplant recipients for the first time from February 2014 to February 2016 were studied in this work. Recipients can be divided into rejection group (=12) and stable renal function group (=41) according to whether rejection occurred.

WSTF promotes proliferation and invasion of lung cancer cells by inducing EMT via PI3K/Akt and IL-6/STAT3 signaling pathways.

Williams syndrome transcription factor (WSTF), which is encoded by the BAZ1B gene, was first identified as a hemizygously deleted gene in patients with Williams syndrome. WSTF protein has been reported to be involved in transcription, replication, chromatin remodeling and DNA damage response, and also functions as a tyrosine protein kinase. However, the function of WSTF in cancer is not known.

[Cytomegalovirus infection down-regulates the expressions of CD226 and CD16 in blood NK cells of the renal transplant recipient].

Objective: To investigate the effect of cytomegalovirus (CMV) infection after renal transplantation on the expressions of the activation receptors CD226 and sCD16 in natural killer (NK) cells.

Methods: Peripheral blood samples were obtained from these kidney transplant recipients with CMV infection, stable recipients, and normal healthy controls, and the expressions of CD3, CD56, CD16 and CD226 of all the samples were analyzed by flow cytometry.

Results: Compared with patients recovered from CMV infection, normal healthy controls and the stable recipients, the percentage of NK population in lymphocytes did not vary among these groups (P=0.

[Effects of different processing methods and durations of blood specimens on the level of soluble CD100 in peripheral blood].

Objective: To investigate the effects of different processing methods and durations of blood specimens on the level of soluble CD100 (sCD100) in peripheral blood.

Methods: We included a total of 15 healthy individuals without any significant signs or symptoms of microbial infection. Blood samples were collected in tubes with and without additives, as well as tubes containing citrate, EDTA and heparin, respectively, and processed for different durations of time.

Identification of miRNomes in human stomach and gastric carcinoma reveals miR-133b/a-3p as therapeutic target for gastric cancer.

Gastric cancer (GC) is the fourth most frequent malignant disease and the second leading cause of cancer mortality worldwide, but the molecular mechanisms underlying this clinically heterogeneous disease are complex and remain far from completely understood. Accumulating evidence suggests that abnormal microRNA (miRNA) expression is involved in tumorigenesis. However, their accurate expression pattern, function, and mechanism in GC remain unclear.

Paris Saponin VII Inhibits the Migration and Invasion in Human A549 Lung Cancer Cells.

Metastasis is the main cause of death in lung cancer. Targeting the process of metastasis is a strategy to lung cancer treatment. Trillium tschonoskii Maxim.

ING5 inhibits cancer aggressiveness via preventing EMT and is a potential prognostic biomarker for lung cancer.

The proteins of the Inhibitor of Growth (ING) candidate tumor suppressor family are involved in multiple cellular functions such as cell cycle regulation, apoptosis, and chromatin remodeling. ING5 is the new member of the family whose actual role in tumor suppression is not known. Here we show that ING5 overexpression in lung cancer A549 cells inhibited cell proliferation and invasiveness, while ING5 knockdown in lung cancer H1299 cells promoted cell aggressiveness.

Trillium tschonoskii steroidal saponins suppress the growth of colorectal Cancer cells in vitro and in vivo.

Ethnopharmacological Relevance: Saponins of many herbs are known to possess anti-cancer effect.

Aim Of The Study: The present study aimed to investigate the growth inhibitory effect of Trillium tschonoskii steroidal saponins in a mouse model of colitis-associated colorectal cancer and a human colorectal cancer cell line HT-29, and isolate some major constituents and evaluate their anti-tumor activity.

Materials And Methods: Forty male ICR mice were administered with 1, 2-dimethyl-hydrazine (DMH) and dextran sodium sulfate (DSS).

[Frequency analyses of human cytomegalovirus specific CD8(+) T cell in kidney transplantation].

Objective: To establish a new detection method for cytomegalovirus (CMV) specific cytotoxic CD8(+)T cells and examine its proportion and significance in peripheral blood from kidney transplant recipients.

Methods: A total of 30 recipients of kidney transplantation from January 2009 to December 2010 for the first time were enrolled. And 10 healthy volunteers were selected as health control group.

Paris saponin VII from trillium tschonoskii reverses multidrug resistance of adriamycin-resistant MCF-7/ADR cells via P-glycoprotein inhibition and apoptosis augmentation.

Ethnopharmacological Relevance: Saponins of several herbs are known to induce apoptosis in some cancer cells and are proposed to be promising modulators of drug resistance. In the present study, we extracted Paris saponin VII (PS VII), a kind of saponin, from Trillium tschonoskii Maxim. and observed its effect on adriamycin-resistant breast cancer cells.

Paris saponin VII inhibits growth of colorectal cancer cells through Ras signaling pathway.

Dysregulation of the Ras signaling pathway plays a key role in the progression of colorectal cancer. When bound to GTP, Ras is activated and stimulates several downstream effectors' pathways, including the Raf/MEK/ERK kinase cascade, the PI3-kinase/AKT/mTor pathway, and the Ral GTPase pathway. Saponins extracted from Liliaceae family herbs have strong antitumor activities with low toxicity.

Should GLP-1 receptor agonists be used with caution in high risk population for colorectal cancer?

Glucagon-like peptide-1 (GLP-1) receptor agonists, with few reported side effects, are a kind of very promising anti-diabetes drugs. They are thus being paid high hopes on by both doctors and patients. GLP-1 receptor agonists, however, have not been used in clinic for a long time.

[A case of hemophagocytic lymphohistiocytosis syndrome caused by severe tuberculosis and literature review].

Objective: To investigate the clinical features of a case of hemophagocytic lymphohistiocytosis syndrome (abbreviated as hemophagocytic syndrome, HPS) caused by severe tuberculosis and therefore to improve early diagnosis of this condition.

Methods: The clinical features and process of diagnosis and treatment of a case with HPS caused by severe tuberculosis in July 2011 were analyzed, and the reported literatures of the disease were reviewed.

Results: The patient was a 16-year-old male.

[Outcomes of anterior versus posterior instrumentation under different surgical procedures in the treatment of thoracolumbar spinal tuberculosis in adults].

Objective: To compare the outcomes of anterior verus posterior instrumentation under different surgical procedures in the surgical management of thoracolumbar spinal tuberculosis (TB).

Methods: Between January 2004 and December 2009, 241 adult patients with thoracolumbar spinal TB underwent radical debridement and strut grafting plus anterior or posterior instrumentation in single-stage or two-stages. The mean age was 39 years (range: 16 - 67).

[Identification and functional study of Tim-1(+)CD19(+) regulatory B cell in kidney transplantation recipients].

Objective: To observe the ratio of Tim-1(+)CD19(+) B cell in the peripheral blood of kidney transplantation recipients and elucidate its functions.

Methods: From December 2009 to June 2010, a total of 35 pairs of kidney transplant recipients were selected and divided into 3 groups: healthy donors as control (n = 35), pre-transplantation (n = 35) and post-transplantation (n = 35). The profiles of Tim-1(+)CD19(+) B cell in kidney transplantation donors and recipients were analyzed and sorted by flow cytometry (FCM).

[Clinical value of low-frequency repetitive nerve stimulation in myasthenia gravis].

Objective: To explore the value of low-frequency repetitive nerve stimulation (RNS) in the diagnosis of myasthenia gravis (MG).

Methods: A total of 436 MG patients admitted during the period of July 2008 and August 2010 and undergoing the repetitive nerve stimulation test were reviewed retrospectively. We analyzed the positive rates of RNS in MG patients and compared the abnormalities in different nerves including facial, accessory, axillary and ulnar nerves.

[Prepared the monoclonal antibody associated with hepatocellular carcinoma and analyzed its epitope].

Aim: To prepare the monoclonal antibodies associated with hepatocellular carcinoma (HCC) for diagnosis.

Methods: 3 BALB/c mice were immunized with high metastasis characteristic HCC cells (HCCLM-6). The splenocytes from the immunized mice were fused with murine myeloma cells (Sp2/0).

[Up-regulation of the sHLA-G5 level by human-mouse chimeric anti-IL-2R monoclonal antibody treatment in kidney transplantation].

Objective: To find whether the up-regulation of soluble human leucocyte antigen-G5 (sHLA-G5) levels is a new function mechanism of anti-interleukin-2 receptors (anti-IL-2R) monoclonal antibody treatment in kidney transplantation.

Methods: A total of 215 recipients at our centre from January 2006 to December 2007 were divided into antibody use group (n = 141) and antibody non-use group (n = 74) and another healthy group (n = 69). The sHLA-G5 level in peripheral blood was detected by enzyme-linked immunosorbent assay (ELISA).

[Applications of monitoring human leukocyte antigen G and its inhibitory receptors in post-renal transplantation].

Objective: to study the feasibility of human leucocyte antigen-G (HLA-G) as a post-transplantation prognostic biomarker and discuss the correlation of its receptor expression and the mechanisms.

Methods: a total of 215 recipients in our centre from February 2006 to June 2008 were divided into stable kidney function group (n = 173) and acute rejection group (n = 42). The soluble human leucocyte antigen-G5 (sHLA-G5) level in peripheral plasma was detected by ELISA.

A microdissection study of perforating vessels in the perineum: implication in designing perforator flaps.

The objective of this study is to determine the quantity, position, and caliber of perforating vessels in the perineum, and to provide an anatomic basis for designing perineal perforator flaps. Eleven adult cadavers (22 sides) were dissected under an operating microscope (x10). Microstructures, including perforating arteries, vena comitantes, vascular anastomoses, and cutaneous nerves, were measured with a sliding caliper (accurate to 0.

[The relation between MSI and the clinicopathologic factors of NSCLC.].

Background: To explore the relationship between microsatellite instability (MSI) and various clinicopathologic factors in non-small cell lung cancer (NSCLC) .

Methods: Ninety-four lung cancer specimens resected from patients with NSCLC were investigated. We used a very precise microdissection technique to obtain the genomic DNA from tumor tissue and paired normal tissue , followed by PCR amplification of 4 polymorphic genomic sequences (D3S1067 ,D3S659 ,D3S966 and AR) .