NSUN2-Mediated R-loop Stabilization as a Key Driver of Bladder Cancer Progression and Cisplatin Sensitivity.

R-loops are critical structures that play pivotal roles in regulating genomic stability and modulating gene expression. This study investigates the interactions between the 5-methylcytosine (mC) methyltransferase NOP2/Sun RNA methyltransferase 2 (NSUN2) and R-loops in the transcriptional dynamics and damage repair process of bladder cancer (BCa) cells. We observed markedly elevated levels of R-loops in BCa cells relative to normal urothelial cells.

Single-Cell RNA Sequencing Reveals the Tumor Heterogeneity and Immunosuppressive Microenvironment in Urothelial Carcinoma.

Urothelial carcinoma (UC) can arise from either the lower urinary tract or the upper tract; they represent different disease entities and require different clinical treatment strategies. A full understanding of the cellular characteristics in UC may guide the development of novel therapies. Here, we performed single-cell transcriptome analysis from four patients with UC of the bladder (UCB), five patients with UC of the ureter (UCU), and four patients with UC of the renal pelvis (UCRP) to develop a comprehensive cell atlas of UC.

Primary ovarian insufficiency consequence of autoimmune diseases: a bidirectional two-sample Mendelian randomization study.

Background: Observational studies suggest the risk of primary ovarian insufficiency (POI) is increased in autoimmune disorders (AIDs), but it is unclear whether there is a causal relationship. Therefore, we aimed to investigate the bidirectional causality between 20 AIDs and POI using Mendelian randomization (MR) analysis.

Methods: A bidirectional two-sample MR investigation was designed by using publicly accessible summary-level data from genome-wide association studies (GWAS).

Lycopene's therapeutic mechanisms in epididymitis: a network pharmacology and experimental study.

Background: Epididymitis, a common disease of the male reproductive system, is often caused by nonspecific infections. Antibiotics alone cannot reverse histopathological changes or prevent long-term reproductive issues. Lycopene (LYC), a potent antioxidant, has shown potential in alleviating epididymitis, yet its specific mechanisms remain unclear.

Clinical and molecular prognostic nomograms for patients with papillary renal cell carcinoma.

Objective: To summarize the clinicopathological characteristics and prognostic factors of papillary renal cell carcinoma (pRCC) and to construct clinical and molecular prognostic nomograms using existing databases.

Methods: Clinical prognostic models were developed using the Surveillance, Epidemiology, and End Results (SEER) database, while molecular prognostic models were constructed using The Cancer Genome Atlas (TCGA) database. Cox regression and LASSO regression were employed to identify clinicopathological features and molecular markers related to prognosis.

Advances of New Extracellular Vesicle Isolation and Detection Technologies in Cancer Diagnosis.

Cancer is a global health issue threatening people's lives. Currently, cancer detection methods still have a lot of room for improvement in both efficiency and accuracy. The development and application of new technologies are urgently required for early cancer diagnosis and prognosis.

Erratum: Cyclovirobuxine inhibits the progression of clear cell renal cell carcinoma by suppressing the IGFBP3-AKT/STAT3/MAPK-Snail signalling pathway: Erratum.

[This corrects the article DOI: 10.7150/ijbs.62114.

Acetyltransferase NAT10 promotes an immunosuppressive microenvironment by modulating CD8 T cell activity in prostate cancer.

N-acetyltransferase 10 (NAT10), an enzyme responsible for ac4C acetylation, is implicated in cancer progression, though its specific biological function in prostate cancer remains insufficiently understood. This study clarifies NAT10's role in prostate cancer and its effects on the tumor immune microenvironment. NAT10 expression and clinical relevance were assessed through bioinformatics, RT-qPCR, and IHC analyses, comparing prostate cancer tissues with normal controls.

Tumor Microenvironment in Clear Cell Renal Cell Carcinoma: A Comprehensive Analysis.

Background: M6A RNA methylation and the tumor microenvironment (TME) have been reported to play important roles in the progression and prognosis of clear cell renal cell carcinoma (ccRCC). However, whether m6A RNA methylation regulators affect the TME in ccRCC remains unknown. Thus, we aimed to evaluate comprehensively the effect of m6A RNA methylation regulators on the TME in ccRCC.

DLX5 Promotes Radioresistance in Renal Cell Carcinoma by Upregulating c-Myc Expression.

Background: Renal cell carcinoma (RCC) is a prevalent and aggressive kidney cancer with notable metastatic potential. While radiotherapy is effective for treating metastatic RCC, the emergence of radioresistance presents a major challenge. This study explores the role of , previously identified as an oncogene in various cancers, in the development of radioresistance in RCC.

Improving signal-to-noise ratio of Raman measurements based on ensemble learning approach.

Raman spectroscopy is an extensively explored vibrational spectroscopic technique to analyze the biochemical composition and molecular structure of samples, which is often assumed to be non-destructive when carefully using proper laser power and exposure time. However, the inherently weak Raman signal and concurrent fluorescence interference often lead to Raman measurements with a low signal-to-noise ratio (SNR), especially for biological samples. Great efforts have been made to develop experimental approaches and/or numerical algorithms to improve the SNR.

Advancements and challenges of R-loops in cancers: Biological insights and future directions.

R-loops involve in various biological processes under human normal physiological conditions. Disruption of R-loops can lead to disease onset and affect the progression of illnesses, particularly in cancers. Herein, we summarized and discussed the regulative networks, phenotypes and future directions of R-loops in cancers.

Single center evaluation of sensitivity and specificity of CellDetect assay in early bladder cancer patients.

Until recently, the main diagnostic methods for bladder cancer (BC) are still voided urine cytology and cystoscopy, and many drawbacks persist. In this retrospective study, we evaluated the sensitivity and specificity of the CellDetect assay in the detection of BC with comparison to standard diagnostic methods. Between August 2020 and July 2022, B-ultrasonography or computed tomography (CT) scan was performed for patients with hematuria or irritative voiding symptoms.

Enhancing under-urine adhesion and bladder adaptation of silk fibroin hydrogels with tea polyphenols for hemorrhagic cystitis.

Hemorrhagic Cystitis (HC) presents a significant therapeutic challenge due to the dynamic fluid environment and cyclical mechanical stress within the bladder. Tissue-adhesive hydrogels have shown promise in treating HC; however, maintaining strong adhesion and mechanical integrity under these fluctuating conditions remains a critical obstacle. Herein, we designed a robust bladder-adhesive hydrogel by leveraging the affinity of tea polyphenols (TP) for damaged tissues and their ability to rapidly enhance the stability of photo-crosslinked silk fibroin methylacryloyl (SFMA) through abundant hydrogen bonding.

Edaravone mitigates calcium oxalate-induced renal tubular epithelial cell injury by inhibiting autophagy-mediated ferroptosis.

Edaravone (EDA) has been found to exert protective effects on kidney injury. Nevertheless, the functions of EDA in kidney stones as well as the potential mechanism are vague. Calcium oxalate (CaOx) was used to induce kidney stones cell model with human renal tubular epithelial cell line HK-2.

Knowledge, attitude, and practice regarding stone formation and recurrence among urolithiasis patients: a cross-sectional study.

This study investigated the knowledge, attitude, and practice (KAP) concerning stone formation and recurrence among patients with urolithiasis treated at Ningbo Medical Centre Lihuili Hospital between August 19, 2023, and April 19, 2024. A total of 394 patients were included, among which 70.30% were males.

RON receptor tyrosine kinase as a critical determinant in promoting tumorigenic behaviors of bladder cancer cells through regulating MMP12 and HIF-2α pathways.

The RON receptor tyrosine kinase is critical in the pathogenesis of various cancer types, however, its role in bladder cancer invasive growth is still largely unknown. Here, we found that over 90% of bladder cancer samples exhibit elevated levels of RON expression, with significantly higher expression levels observed in invasive bladder cancer compared to non-invasive bladder cancer. In vitro, RON activation resulted in increased bladder cancer cell migration and invasiveness.

High-throughput and high-sensitive direct detection for pathogenic bacteria of urinary tract infections mediated by block-based design of flexible non-metallic composite SERS substrate.

It remains a huge challenge to realize a high-throughput direct detection for pathogenic bacteria with high-sensitivity in practice. Here, we develop a typical two-dimensional (2D) composite semiconductor of BP@MoS with special synergistic chemical enhancement-mediated surface-enhanced Raman scattering (SERS) activity. The relative proportion of MoS and BP was rationally adjusted in the hydrothermal reaction to screen a composite sample with high charge transfer efficiency.

Oxidative balance score and the potential for suffering rheumatoid arthritis: a cross-sectional study.

Objective: Our study was conducted to explore the link between oxidative balance score (OBS) and rheumatoid arthritis (RA).

Methods: A total of 21,415 participants were included in our research from five cycles (2011-2012, 2013-2014, 2015-2016, 2017-2018, and 2017-2020) of the National Health and Nutrition Examination Survey (NHANES). Moreover, 20 elements related to diet as well as lifestyle were combined to calculate OBS.

Role of repeat transurethral resection in no-muscle-invasive bladder tumour: an umbrella review.

Background: Repeat transurethral resection of bladder tumour (reTURB) is a conventional treatment for non-muscle-invasive bladder cancer (NMIBC) to enhance prognosis. However, the necessity of reTURB in NMIBC remains controversial owing to upstaging of treatments and new evidence.

Objectives: We performed an umbrella review to determine the need for reTURB in patients with NMIBC.

Pan-cancer analysis of oncogenic role of CEP55 and experiment validation in clear cell renal cell carcinoma.

Immunotherapy has emerged as a vital component in the contemporary landscape of cancer treatment. Recent studies have indicated that CEP55 plays an oncogenic role; however, its specific mechanisms in promoting tumor proliferation and its potential value in prognosis and immunotherapy prediction across various cancers remain to be elucidated. CEP55 was significantly overexpressed in 22 cancer types compared with their adjacent normal tissues.