Synthesis and properties of acridone-labeled base-discriminating fluorescent (BDF) nucleosides.

We have developed novel acridone-labelled BDF probe which showed its potential in recognizing opposite matched base from its target sequence via enhancement of fiuorescence intensity. This probe emit at a longer wavelength than previously reported pyrene-labelled BDF probe and thus can be used in DNA chip.

Synthesis and properties of novel base-discriminating fluorescent (BDF) nucleosides.

We designed a new type of pyrene-labeled base-discrimination fluorescent (BDF) nucleosides (Py)U, (Py)C, (8Py)A and (MePy)dA, which emitted strong fluorescence only when the bases opposite the BDF base are A, G, T and C, respectively. The DNA probes containing four different BDF bases enable us to distinguish single base alterations by simply mixing with a sample solution of target DNA.

Design of base-discriminating fluorescent (BDF) nucleobase for SNP typing.

We have devised novel pyrene-labeled BDF nucleosides, (Py)U, (Py)C, (Py)deazaA, and (Py)A. BDF probes containing these fluorescent nucleosides selectively emit fluorescence only when the base opposite the BDF base is a target base. Oligonucleotides containing these BDF nucleosides act as effective reporter probes for homogeneous SNP typing of DNA samples.

Base-discriminating fluorescent (BDF) nucleoside: distinction of thymine by fluorescence quenching.

A novel fluorescence BDF probe containing pyrene-labeled 7-deaza-2[prime or minute]-deoxyadenosine has been developed for the detection of thymine base on a target DNA.