Interactions between cholinergic and prostaglandin signaling elements in the urothelium: role for muscarinic type 2 receptors.

Objective: To characterize the interactions between the cholinergic and prostaglandin signaling systems within the urothelium-lamina propria of the guinea pig and elucidate the role of muscarinic receptors in these interactions.

Methods: The urothelium-lamina propria was isolated from guinea pig bladders, cut into strips (5×10 mm), and maintained in vitro. The tissue was either stretched or left unstretched but exposed to 2'(3')-O-(4-benzoylbenzoyl)adenosine-5'-triphosphate tri(triethylammonium) salt, arecaidine, and prostaglandin E2 (PGE2).