Severity: Warning
Message: fopen(/var/lib/php/sessions/ci_sessionpmk2br9j5fr1t3dpf63ak1l5abtq31l9): Failed to open stream: No space left on device
Filename: drivers/Session_files_driver.php
Line Number: 177
Backtrace:
File: /var/www/html/index.php
Line: 316
Function: require_once
Severity: Warning
Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)
Filename: Session/Session.php
Line Number: 137
Backtrace:
File: /var/www/html/index.php
Line: 316
Function: require_once
369 results match your criteria: "New York Structural Biology Center[Affiliation]"
Proc Natl Acad Sci U S A
December 2024
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27599.
Adaptor protein complex-3 (AP-3) mediates cargo sorting from endosomes to lysosomes and lysosome-related organelles. Recently, it was shown that AP-3 adopts a constitutively open conformation compared to the related AP-1 and AP-2 coat complexes, which are inactive until undergoing large conformational changes upon membrane recruitment. How AP-3 is regulated is therefore an open question.
View Article and Find Full Text PDFNat Methods
December 2024
GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, Guangzhou National Laboratory, Guangzhou Medical University, Guangzhou, China.
Trends Pharmacol Sci
November 2024
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY 10027, USA. Electronic address:
Acta Crystallogr F Struct Biol Commun
December 2024
Department of Biology, University of Fribourg, Chemin du Musée 10, 1700 Fribourg, Switzerland.
Nat Struct Mol Biol
November 2024
Department of Physiology and Biophysics, Weill Cornell Medical College of Cornell University, New York, NY, USA.
The heart, in addition to its primary role in blood circulation, functions as an endocrine organ by producing cardiac hormone natriuretic peptides. These hormones regulate blood pressure through the single-pass transmembrane receptor guanylyl cyclase A (GC-A), also known as natriuretic peptide receptor 1. The binding of the peptide hormones to the extracellular domain of the receptor activates the intracellular guanylyl cyclase domain of the receptor to produce the second messenger cyclic guanosine monophosphate.
View Article and Find Full Text PDFMol Cell
December 2024
Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY 10027, USA; Department of Biochemistry and Molecular Biophysics, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USA. Electronic address:
Sevenless (Sev) is a Drosophila receptor tyrosine kinase (RTK) required for the specification of the R7 photoreceptor. It is cleaved into α and β subunits and binds the ectodomain of the G-protein-coupled receptor bride of sevenless (Boss). Previous work showed that the Boss ectodomain could bind but not activate Sev; rather, the whole seven-pass transmembrane Boss was required.
View Article and Find Full Text PDFActa Crystallogr F Struct Biol Commun
December 2024
Dartmouth Cancer Center, One Medical Center Drive, Lebanon, NH 03756, USA.
Acta Crystallogr F Struct Biol Commun
November 2024
Simons Electron Microscopy Center, New York Structural Biology Center, New York, New York, USA.
Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical techniques with calibration standards, cryoEM has lacked comprehensive biological test samples. Here, a cryoEM calibration sample consisting of a mixture of compatible macromolecules is introduced that can not only be used for resolution optimization, but also provides multiple reference points for evaluating instrument performance, data quality and image-processing workflows in a single experiment.
View Article and Find Full Text PDFJ Med Chem
November 2024
Center of Medicinal Chemistry (CQMED), Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas, UNICAMP, 13083-886-Campinas, SP, Brazil.
Nontuberculous mycobacteria (NTM) are emerging human pathogens linked to severe pulmonary diseases. Current treatments involve the prolonged use of multiple drugs and are often ineffective. Bacterial dihydrofolate reductase (DHFR) is a key enzyme targeted by antibiotics in Gram-negative bacterial infections.
View Article and Find Full Text PDFbioRxiv
October 2024
Section of Genetic Medicine, Department of Medicine, Biological Sciences Division, University of Chicago, Chicago, IL, USA.
Small-molecule RNA binders have emerged as an important pharmacological modality. A profound understanding of the ligand selectivity, binding mode, and influential factors governing ligand engagement with RNA targets is the foundation for rational ligand design. Here, we report a novel class of coumarin derivatives exhibiting selective binding affinity towards single G RNA bulges.
View Article and Find Full Text PDFJ Biol Chem
October 2024
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas, USA. Electronic address:
Gram-positive bacteria utilize a Fatty Acid Kinase (FAK) complex to harvest fatty acids from the environment. This complex consists of the fatty acid kinase, FakA, and an acyl carrier protein, FakB, and is known to impact virulence and disease outcomes. Despite some recent studies, there remain many outstanding questions as to the enzymatic mechanism and structure of FAK.
View Article and Find Full Text PDFJ Biol Chem
November 2024
Department of Pediatrics Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA; Microbiology/Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA. Electronic address:
J Immunol
October 2024
Division of Infectious Diseases, Wadsworth Center, New York State Department of Health, Albany, NY.
Lyme disease is a tick-borne, multisystem infection caused by the spirochete Borreliella burgdorferi. Although Abs have been implicated in the resolution of Lyme disease, the specific B cell epitopes targeted during human infections remain largely unknown. In this study, we characterized and defined the structural epitope of a patient-derived bactericidal monoclonal IgG (B11) against outer surface protein C (OspC), a homodimeric lipoprotein necessary for B.
View Article and Find Full Text PDFbioRxiv
August 2024
Laboratory of Structural Biophysics and Mechanobiology, The Rockefeller University, New York, NY, USA.
Cells mechanically interface with their surroundings through cytoskeleton-linked adhesions, allowing them to sense physical cues that instruct development and drive diseases such as cancer. Contractile forces generated by myosin motor proteins mediate these mechanical signal transduction processes through unclear protein structural mechanisms. Here, we show that myosin forces elicit structural changes in actin filaments (F-actin) that modulate binding by the mechanosensitive adhesion protein α-catenin.
View Article and Find Full Text PDFbioRxiv
August 2024
Department of Anesthesiology, Weill Cornell Medical College.
Phosphatidylserine externalization on the surface of dying cells is a key signal for their recognition and clearance by macrophages and is mediated by members of the X-Kell related (Xkr) protein family. Defective Xkr-mediated scrambling impairs clearance, leading to inflammation. It was proposed that activation of the Xkr4 apoptotic scramblase requires caspase cleavage, followed by dimerization and ligand binding.
View Article and Find Full Text PDFbioRxiv
August 2024
University of Kansas School of Medicine, Department of Microbiology, Molecular Genetics & Immunology, Kansas City, Kansas.
In diderm bacteria, the Lol pathway canonically mediates the periplasmic transport of lipoproteins from the inner membrane (IM) to the outer membrane (OM) and therefore plays an essential role in bacterial envelope homeostasis. After extrusion of modified lipoproteins from the IM via the LolCDE complex, the periplasmic chaperone LolA carries lipoproteins through the periplasm and transfers them to the OM lipoprotein insertase LolB, itself a lipoprotein with a LolA-like fold. Yet, LolB homologs appear restricted to γ-proteobacteria and are missing from spirochetes like the tick-borne Lyme disease pathogen , suggesting a different hand-off mechanism at the OM.
View Article and Find Full Text PDFbioRxiv
August 2024
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY 10027, USA.
Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical techniques with calibration standards, cryoEM has lacked comprehensive biological test samples. We introduce a cryoEM calibration sample that is a mixture of compatible macromolecules that can be used not only for resolution optimization but also provides multiple reference points for evaluating instrument performance, data quality, and image processing workflows in a single experiment.
View Article and Find Full Text PDFSci Adv
August 2024
Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
Phytochromes are red-light photoreceptors discovered in plants with homologs in bacteria and fungi that regulate a variety of physiological responses. They display a reversible photocycle between two distinct states: a red-light-absorbing Pr state and a far-red light-absorbing Pfr state. The photoconversion regulates the activity of an enzymatic domain, usually a histidine kinase (HK).
View Article and Find Full Text PDFSci Rep
August 2024
Department of Chemistry, Louisiana State University, Baton Rouge, 70803, USA.
Protein Sci
August 2024
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Saint Louis, Missouri, USA.
ACS Nano
July 2024
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332, United States.
Protein nanoparticles are effective platforms for antigen presentation and targeting effector immune cells in vaccine development. Encapsulins are a class of protein-based microbial nanocompartments that self-assemble into icosahedral structures with external diameters ranging from 24 to 42 nm. Encapsulins from were designed to package bacterial RNA when produced in and were shown to have immunogenic and self-adjuvanting properties enhanced by this RNA.
View Article and Find Full Text PDFNat Methods
August 2024
Department of Computer Science, Princeton University, Princeton, NJ, USA.
Advances in cryo-electron tomography (cryo-ET) have produced new opportunities to visualize the structures of dynamic macromolecules in native cellular environments. While cryo-ET can reveal structures at molecular resolution, image processing algorithms remain a bottleneck in resolving the heterogeneity of biomolecular structures in situ. Here, we introduce cryoDRGN-ET for heterogeneous reconstruction of cryo-ET subtomograms.
View Article and Find Full Text PDFRes Sq
July 2024
Simons Electron Microscopy Center, New York Structural Biology Center, NY, NY, USA.
CryoEM democratization is hampered by access to costly plunge-freezing supplies. We introduce methods, called CryoCycle, for reliably blotting, vitrifying, and reusing clipped cryoEM grids. We demonstrate that vitreous ice may be produced by plunging clipped grids with purified proteins into liquid ethane and that clipped grids may be reused several times for different protein samples.
View Article and Find Full Text PDFActa Crystallogr F Struct Biol Commun
August 2024
Seattle Structural Genomics Center for Infectious Disease (SSGCID), Seattle, WA 98109, USA.
Acta Crystallogr F Struct Biol Commun
August 2024
National Center for CryoEM Access and Training, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY 10027, USA.
Preparation of biomacromolecules for structural biology studies is a complex and time-consuming process. The goal is to produce a highly concentrated, highly pure product that is often shipped to large facilities with tools to prepare the samples for crystallization trials or for measurements at synchrotrons and cryoEM centers. The aim of this article is to provide guidance and to discuss general considerations for shipping biomacromolecular samples.
View Article and Find Full Text PDF