Circulating levels of miR-20b-5p are associated with survival in cardiogenic shock.

Cardiogenic shock (CS) is a medical emergency with high in-hospital mortality. New biomarkers are needed to identify patients at a greater risk of adverse outcomes. This study aimed to investigate the prognostic potential of microRNAs (miRNAs) in assessment of the outcome of cardiogenic shock.

TRPA1 channels modulate cutaneous vasodilation during exercise in the heat in young adults when NOS is inhibited.

Nitric oxide synthase (NOS) is an important mediator of cutaneous vasodilation during exercise-heat stress. We recently reported that pharmacological activation of transient receptor potential ankyrin 1 (TRPA1) channel mediates cutaneous vasodilation via NOS-dependent mechanisms under nonheat stress-resting conditions. Here, we hypothesized that TRPA1 channel activation would contribute to cutaneous vasodilation during exercise in the heat via NOS-dependent mechanisms.

Frontier molecular orbital weighted model based networks for revealing organic delayed fluorescence efficiency.

Free of noble-metal and high in unit internal quantum efficiency of electroluminescence, organic molecules with thermally activated delayed fluorescence (TADF) features pose the potential to substitute metal-based phosphorescence materials and serve as the new-generation emitters for the mass production of organic light emitting diodes (OLEDs) display. Predicting the function of TADF emitters beyond classic chemical synthesis and material characterization experiments remains a great challenge. The advances in deep learning (DL) based artificial intelligence (AI) offer an exciting opportunity for screening high-performance TADF materials through efficiency evaluation.

Unravelling selectivity discrepancies of protoporphyrin binding to glutathione transferase: A comparative analysis of molecular dynamic simulated versus implicit solvent-minimized protein models.

Schistosomiasis is a neglected tropical disease caused by parasitic trematodes, which are an ongoing global health and veterinary concern owing to their acquired drug resistance pressure to available treatment. There is a need for a new generation of effective anthelmintics for preventive and therapeutic purposes. Natural products, such as porphyrins, have been reported to inhibit the main detoxification enzymes in these parasites, called glutathione transferases, which help them evade immune response and drug therapy, thus making them good drug targets.

Genetic interaction between oxidative stress and body mass index in a Spanish population.

Oxidative stress may act as a contributing factor in the development of an elevated body mass index (BMI). Oxidative stress has the potential to modulate genetic activity at various levels, including gene transcription and protein function regulation. Nevertheless, the interplay between genetic variants and oxidative stress in relation to BMI remains to be elucidated.

Large range sizes link fast life histories with high species richness across wet tropical tree floras.

Understanding how the traits of lineages are related to diversification is key for elucidating the origin of variation in species richness. Here, we test whether traits are related to species richness among lineages of trees from all major biogeographical settings of the lowland wet tropics. We explore whether variation in mortality rate, breeding system and maximum diameter are related to species richness, either directly or via associations with range size, among 463 genera that contain wet tropical forest trees.

Ultrasound stimulated piezoelectric antibacterial silk composite films guiding differentiation of mesenchymal stem cells.

Smart materials for tissue engineering have been in extensive use for few decades now. This work delves into the exploration of ultrasound-stimulated piezoelectric and antibacterial silk-based composite films as a pioneering strategy to guide the differentiation of human mesenchymal stem cells into osteogenic lineage without the application of any exogenous growth factors. The study evaluates the biocompatibility and antibacterial attributes of these films, which incorporates Barium Titanate nanoparticles (BTNPs) along with Zinc Oxide nanoparticles for obtaining high piezo modulated stimuli response and antibacterial properties.

Numerical assessment of the performance of a new multi side-hole catheter design in liver radioembolization.

Numerical fluid-particle dynamics simulations, such as Computational Fluid Dynamics (CFD), are widely used to study blood flow and microsphere transport in medical treatments like radioembolization (RE), a therapy for unresectable liver tumors. RE involves injecting 90Y-labeled microspheres via a microcatheter into a hepatic artery to irradiate cancer cells. This study uses simulations on three patient-specific hepatic arteries to evaluate microsphere distribution in RE, focusing on two catheter designs: a standard end-hole catheter (EHC) and a novel multi side-hole catheter (SHC).

Apolipoprotein C3 and risk of cardiovascular events and death in patients on optimized statin treatment after recent acute coronary syndrome.

Aims: Apolipoprotein (Apo) C3 has been associated with incident coronary heart disease and major adverse cardiovascular events (MACE). Whether ApoC3 levels predict risk in patients with acute coronary syndrome (ACS) on optimized statin treatment is unknown.

Methods: ApoC3 was measured by mass spectrometry at baseline (n=11,956) and after 4 months' treatment (M4; n=11 176) with alirocumab or placebo in the ODYSSEY OUTCOMES trial.

Two-Dimensional Perovskites for Photocatalytic CO Reduction.

The photocatalytic conversion of Carbon dioxide (CO) into valuable chemicals is one of the most promising approaches to addressing the CO emission problem. However, several issues still need to be resolved to increase the efficiency of photocatalytic reactions. Perovskites possess superior light absorption capacity, tunable band gaps, high defect tolerance, and diverse dimensionality.

Biodegradable ultrahigh-purity magnesium and its alloy ZX00 promote osteogenesis in the medullary cavity and glycogenolysis in the liver.

Magnesium (Mg)-based implants have become an attractive alternative to conventional permanent implants in the orthopedic field. While biocompatibility, degradation kinetics, and osseointegration of Mg-based implants have been mostly investigated, the impact of degradation products on bone remodeling and potential systemic effects remains unclear. The aim of this study was to evaluate the early and mid-term local and systemic tissue responses of degrading ultrahigh-purity ZX00 (Mg-Zn-Ca alloy) and ultrahigh-purity Mg (XHP-Mg) pins in a juvenile healthy rat model.

New insights on antibacterial mode of action of blue-light photoactivated berberine and curcumin-antibiotic combinations against Staphylococcus aureus.

Antimicrobial photodynamic inactivation (aPDI), using photosensitisers in combination with antibiotics, is a promising multi-target strategy to address antibiotic resistance, particularly in wound infections. This study aimed to elucidate the antibacterial mode of action of combinations of berberine (Ber) or curcumin (Cur) with selected antibiotics (Ber-Ab or Cur-Ab) under blue light irradiation (420 nm) against Staphylococcus aureus, including methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains. Multiple physiological parameters were assessed using complementary assays (fluorometry, epifluorescence microscopy, flame emission and atomic absorption spectroscopy, zeta potential, flow cytometry, and the plate agar method) to examine the effect on ROS production, membrane integrity, DNA damage, motility and virulence factors of S.

Modulating the developing gut microbiota with 2'-fucosyllactose and pooled human milk oligosaccharides.

Background: Synthetic human milk oligosaccharides (HMOs) are used to supplement infant formula despite limited understanding of their impact on the post-weaned developing gut microbiota. Here, we assess the influence of 0.5 g/L 2-fucosyllactose (2'FL) and 4.

Malaria parasite cysteine and aspartic proteases as key drug targets for antimalarial therapy.

Context: Cysteine and aspartic proteases are enzyme families that play crucial roles in the life cycle of Plasmodium, the parasite responsible for malaria. These proteases are involved in vital biological processes, such as hemoglobin degradation within the host's red blood cells, protein turnover, and regulation of parasite development. Inhibiting these proteases with small molecule drugs can block the parasite's growth and survival.

Different chemical scaffolds bind to L-phe site in Mycobacterium tuberculosis Phe-tRNA synthetase.

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mt), is one of the deadliest infectious diseases. The rise of multidrug-resistant strains represents a major public health threat, requiring new therapeutic options. Bacterial aminoacyl-tRNA synthetases (aaRS) have been shown to be highly promising drug targets, including for TB treatment.

Mapping physiology: A systems biology approach for the development of alternative methods in toxicology.

Chemical safety assessment still heavily relies on animal testing, presenting ethical dilemmas and limited human predictive value. New approach methodologies (NAMs), including in vitro and in silico techniques, offer alternative solutions. In silico toxicology has made progress in predicting chemical effects but frequently lacks biological mechanistic foundations.

Research on Neuroimmune Gastrointestinal Diseases Based on Artificial Intelligence: Molecular Dynamics Analysis of Caffeine and DRD3 Protein.

Objectives: The aim of this study was to develop a clinical application model for the rational use of caffeine.

Background: Caffeine is related to the incidence of neuro immune gastrointestinal diseases. Coffee consumption needs to be optimized in order to reduce the incidence rate.

Therapeutic effects of NAC and CoQ10 on aluminium phosphide poisoning as an adjuvant therapy: A Pilot study.

Unlabelled: In aluminium phosphide (AlP) poisoning, death is mainly due to acute heart failure. There is some evidence showing that N-acetylcysteine (NAC) and coenzyme Q10 have antioxidant and cardioprotective effects. This study investigated a new approach for treating acute AlP poisoning by using NAC and Co-Q10 as adjuvant therapy.

Role of plasma metabolome in mediating the effect of plasma lipidome on NAFLD: a Mendelian randomization study.

Background: This study explored the causal connection among the plasma lipidome, nonalcoholic fatty liver disease (NAFLD), and potential metabolome mediators through Mendelian randomization (MR).

Methods: We obtained summary statistics for 179 plasma lipidome traits ( = 7,174), 1,400 plasma metabolome traits ( = 8,299), and one NAFLD trait from publicly available genome-wide association studies. A two-sample MR analysis was conducted to infer causality.

TIMSRescore: A Data Dependent Acquisition-Parallel Accumulation and Serial Fragmentation-Optimized Data-Driven Rescoring Pipeline Based on MSRescore.

The high throughput analysis of proteins with mass spectrometry (MS) is highly valuable for understanding human biology, discovering disease biomarkers, identifying therapeutic targets, and exploring pathogen interactions. To achieve these goals, specialized proteomics subfields, including plasma proteomics, immunopeptidomics, and metaproteomics, must tackle specific analytical challenges, such as an increased identification ambiguity compared to routine proteomics experiments. Technical advancements in MS instrumentation can mitigate these issues by acquiring more discerning information at higher sensitivity levels.

A Rhodamine-and-Naphthalimide Based Dual-chromophore for Fast and Sensitive Detection of Nerve-agent Mimic and Real Nerve Agents.

For the sake of public safety and citizen health, the development of efficient and portable detection devices to nerve agents is critical. In this investigation, a naphthalimide-rhodamine based dual-chromophore, RNI-P, was synthesized and applied to detect nerve-agent mimic diethyl chlorophosphate (DCP) and real nerve agents. The probe exhibits inherently weak fluorescence due to the PET processes from both piperazine and rhodamine units to the naphthalimide unit.

Number of people treated for hepatitis C virus infection in 2014-2023 and applicable lessons for new HBV and HDV therapies.

Background And Aims: The year 2023 marked the 10-year anniversary of the launch of direct-acting antivirals (DAAs) for the treatment of the hepatitis C virus (HCV). HCV treatment trends by country, region, and globally are important to monitor progress toward the World Health Organization's 2030 elimination targets. Additionally, the historical patterns can help predict the treatment uptake for future therapies for other liver diseases.

Escherichia coli O157:H7 multiplication in the latex of diverse lettuce genotypes is negatively correlated with plant peroxidase activity.

Lettuce (Lactuca spp.) is one of few edible plant species that produce latex. During lettuce harvest, latex leaks from ruptured laticifers onto the cut stem and adheres to other lettuce heads, harvesting tools, and packaging.

Ecotoxicological responses of marine fish to the organophosphate flame-retardant tris (2-chloroisopropyl) phosphate (TCPP) dietary exposure: Juvenile gilthead seabream (Sparus aurata) as a case study.

High Production Volume Chemicals (HPVCs) are contaminants that pose serious threats to aquatic environments and species that inhabit them, given their massive production and ubiquitous distribution across biological compartments. Among them, organophosphate esters (OPEs) are of particular concern, as they are widely used as plasticizers and flame-retardants, and linked to various forms of toxicity in marine organisms. In this study, we investigated the ecotoxicological response of juvenile gilthead seabream Sparus aurata to the OPE tris (2-chloroisopropyl) phosphate (TCPP) following chronic dietary exposure to three different concentrations (low, D1: 0.

Risk of Incident Diabetes Related to Lipoprotein(a), LDL Cholesterol, and Their Changes With Alirocumab: Post Hoc Analyses of the ODYSSEY OUTCOMES Randomized Trial.

Objective: Previous genetic and clinical analyses have associated lower lipoprotein(a) and LDL cholesterol (LDL-C) with greater risk of new-onset type 2 diabetes (NOD). However, PCSK9 inhibitors such as alirocumab lower both lipoprotein(a) and LDL-C without effect on NOD.

Research Design And Methods: In a post hoc analysis of the ODYSSEY OUTCOMES trial (NCT01663402), we examined the joint prediction of NOD by baseline lipoprotein(a), LDL-C, and insulin (or HOMA-insulin resistance [HOMA-IR]) and their changes with alirocumab treatment.