Omics data integration analysis identified new biological insights into chronic antibody-mediated rejection (CAMR).

Background: In the last two decades, many studies based on omics technologies have contributed to defining the clinical, immunological, and histological fingerprints of chronic antibody-mediated rejection (CAMR), the leading cause of long-term kidney allograft failure. However, the full biological machinery underlying CAMR has only been partially defined, likely due to the fact thatsingle-omics technologies capture only specific aspects of the biological system and fail to provide a comprehensive understanding of this clinical complication.

Methods: This study integrated mass spectrometry-based proteomic profiling of serum samples from 19 patients with clinical and histological evidence of CAMR and 26 kidney transplant recipients with normal graft function and histology (CTR) with transcriptomic analysis of peripheral blood mononuclear cells (PBMCs) from an independent cohort of 10 CAMR and 8 CTR patients.

Exploring immunotherapy with antibody-drug conjugates in solid tumor oncology.

Immunotherapy has emerged as a hallmark of hope in the formidable battle against solid tumors such as breast cancer, colorectal cancer, etc., with antibody-drug conjugates (ADCs) starting a new era of precision medicine. This chapter delves into the dynamic landscape of immunotherapeutic strategies, focusing on the transformative potential of ADCs.

Salts Influence IDP Properties by Modulating the Population of Conformational Clusters.

Salts readily alter the physical properties of intrinsically disordered proteins (IDPs) rich in charged residues. Using a coarse-grained IDP model and computer simulations, we investigated how salts affect the heterogeneous conformational ensemble and segment-level structures of the IDP prothymosin-α, classified as a polyelectrolyte. We show that clusters of conformations with distinct structural features are present within the conformational ensemble of prothymosin-α by projecting it onto a two-dimensional latent space with the aid of autoencoders.

Neuronal FAM171A2 mediates α-synuclein fibril uptake and drives Parkinson's disease.

Neuronal accumulation and spread of pathological α-synuclein (α-syn) fibrils are key events in Parkinson's disease (PD) pathophysiology. However, the neuronal mechanisms underlying the uptake of α-syn fibrils remain unclear. In this work, we identified as a PD risk gene that affects α-syn aggregation.

Bone Marrow CD8 + Abundance Inversely Correlates with Progressive Marrow Fibrosis and Myelodysplastic Evolution in GATA2 Deficiency: Case Report.

Purpose: GATA2 deficiency, a rare inborn error of immunity, presents with highly variable phenotypes. Bone marrow (BM) changes such as hypocellularity and myelodysplastic syndrome (MDS) are common, with hematopoietic stem cell transplantation being the only curative option due to the risk of progression to acute myeloid leukemia. Although traditional markers like cytogenetic abnormalities and somatic mutations (e.

Luminescent Cd(II) Fumarate Bridging 1D Coordination Polymer: Ultra-Trace Level Detection of Cu in Aqueous Medium and Fabrication of Semiconducting Device.

In addressing the Sustainable Development Goals (SDGs) the UNDP has fixed 17 issues out of them clean water (SDG-6) and affordable energy (SDG-7) serve as decisive points towards sustenance of the society. Material chemistry plays a vital role to design new chemical compounds and exploring versatile activities towards the solution of pressing challenges. Copper, third abundant metal in human body following iron and zinc, is useful to monitor many metabolic processes.

Polariton spectra under the collective coupling regime. II. 2D non-linear spectra.

In our previous work [Mondal et al., J. Chem.

New Fluorescent Chemodosimetric Mechanism for Selective Recognition of Selenocysteine by Dansyl-Appended Ruthenium Nitrosyl Complexes.

Selenocysteine (Sec) is a biologically essential amino acid that serves as a crucial component in selenoproteins that play a key role in various cellular functions. Thus, developing a reliable and rapid method for detecting Sec in physiological media is of paramount importance. This report introduces for the first time a novel fluorescent chemodosimetric mechanism for the selective recognition of Sec using dansyl-appended ruthenium nitrosyl complexes.

Thermally Activated Delayed Fluorescence of Organic-Inorganic Hybrid Menshutkin-Type Cu-Sb Halides.

Organic-inorganic hybrid metal halides have attracted much attention due to their excellent optoelectronic properties. The heterometallic structure is expected to achieve more complex luminescence centers, thereby improving the tunability of the fluorescent materials. This paper reports the synthesis of crystals [Cu(2,2'-bpy)(PPh)][CuCl(PPh)SbOCl] (, 2,2'-bpy = 2,2'-bipyridine, PPh = triphenylphosphine) and (Mim)[SbOCl] (, Mim = 1-methylimidazole).

A galactose-tethered tetraphenylethene prodrug mediated apoptosis of senescent cells for osteoporosis treatment.

Osteoporosis and bone injury healing in elderly patients are major medical challenges, often exacerbated by the accumulation of senescent cells. Herein, we show that TPE-Gal, which contains a tetraphenylethene unit and a galactose moiety, offers a promising molecular therapy designed to light up and eliminate senescent cells through a hydrolysis reaction catalyzed by β-galactosidase, an enzyme overexpressed in senescent cells. The reaction produces TPE-OH, which, in turn, increases reactive oxygen species levels within the senescent cells, leading to noninflammatory apoptosis of senescent cells.

From duplication to fusion: Expanding Dayhoff's model of protein evolution.

Dayhoff's hypothesis suggests that complex proteins emerged from simpler peptides or domains, which duplicated and fused to create more complex proteins and novel functions. These processes expanded and diversified the protein repertoire within organisms. Extensive studies and reviews over the past two decades have highlighted the impact of gene duplication on protein evolution.

Strategic Synthesis of Mixed-Phase Tungsten Oxide for Electrochromic Smart Windows.

Electrochromic devices based on mixed-phase WO can potentially outperform their pure-phase counterparts due to the optimized distribution of optically active sites that facilitate cation intercalation. In this work, we synthesized WO containing orthorhombic and hexagonal phases, with precise phase ratio control accomplished through a meticulously designed experimental strategy of optimizing the reaction time at low temperatures in a closed system under a hydrogen atmosphere. A detailed XRD analysis shows an optimal phase ratio (orthorhombic/hexagonal = 0.

Accurate, comprehensive database of group I introns and their homing endonucleases.

Motivation: Group I introns are one of the most widely studied ribozymes. Since their initial discovery, a large number of them have been identified experimentally or computationally. However, no comprehensive and unified database that provides group I intron sequences with precise boundaries and structural information is available.

Correction: Enhancing the antibacterial efficacy of vancomycin analogues: targeting metallo-β-lactamases and cell wall biosynthesis.

[This corrects the article DOI: 10.1039/D4SC03577A.].

Overcoming beta-lactam resistance in by targeting metallo-beta-lactamase VIM-1: a one-microsecond molecular dynamics simulation study.

is a Gram-negative opportunistic pathogen with a high resistance to beta-lactam antibiotics, mainly due to the production of metallo-beta-lactamase VIM-1 (MBL-VIM-1) enzyme. This study aimed to identify new inhibitors targeting MBL-VIM-1 to restore the efficacy of beta-lactam antibiotics. Extensive screening of natural compounds from the COCONUT database was performed to identify the structural analogs of the existing inhibitor of the MBL-VIM-1 protein.

Discovery and characterization of a human anti-CD36 scFv.

Introduction: CD36 is a membrane receptor that participates in the cellular uptake of fatty acids and lipid metabolism. CD36 overexpression favors progression of different pathologies, such as atherosclerosis and cancer. Thus, CD36 targeting has medicinal relevance.

The influence of different factors on the bond strength of lithium disilicate-reinforced glass-ceramics to Resin: a machine learning analysis.

Background: To assess the influence of various factors on the bond strength of glass-based ceramics and develop a model that can predict the bond strength values using machine learning (ML).

Methods: The bond strength values of lithium disilicate-reinforced glass-ceramics were collected from existing literature. Nineteen features were listed, and 9 ML algorithms, including logistic regression, k-nearest neighbors, support vector machine, decision tree, ensemble methods (extra trees, random forest, gradient boosting, and extreme gradient boosting), and multilayer perceptron, were employed.

Glucagon-like peptide 1 (GLP-1) receptor agonists for people with chronic kidney disease and diabetes.

Background: Approximately 40% of people with diabetes develop kidney failure and experience an accelerated risk of cardiovascular complications. Glucagon-like peptide 1 (GLP-1) receptor agonists are glucose-lowering agents that manage glucose and weight control.

Objectives: We assessed the benefits and harms of GLP-1 receptor agonists in people with chronic kidney disease (CKD) and diabetes.

High-Connectivity 3D Covalent Organic Frameworks with pdp Net for Efficient CH/CO Separation.

High-connectivity 3D covalent organic frameworks (COFs) have garnered significant attention due to their structural complexity, stability, and potential for functional applications. However, the synthesis of 3D COFs using mixed high-nodal building units remains a substantial challenge. In this work, we introduce two novel 3D COFs, JUC-661 and JUC-662, which are constructed using a combination of D-symmetric 8-nodal and D-symmetric 6-nodal building blocks.

Combined inhibition of ribonucleotide reductase and WEE1 induces synergistic anticancer activity in Ewing's sarcoma cells.

Background: Ewing's sarcoma is a childhood bone and soft tissue cancer with poor prognosis. Treatment outcomes for Ewing's sarcoma patients have improved only modestly over the past decades, making the development of new treatment strategies paramount. In this study, the combined targeting of ribonucleotide reductase (RNR) and WEE1 was explored for its effectiveness against Ewing's sarcoma cells.

Direct Observation of Electron Donation onto the Reactants and a Transient Poisoning Mechanism During CO Electroreduction on Ni Single Atom Catalysts.

Single atom catalysts (SACs) are an important class of materials that mediate chemical reduction reactions, a key subset of which is Ni within a carbon support for the electrochemical CO reduction reaction (CORR). However, how the metal atom/clusters and the carbon-based support act in concert to catalyze CORR is not well understood, with most reports attributing activity solely to the Ni-N/C moieties. To address this gap, we have undertaken a mechanistic investigation, employing in situ X-ray absorption spectroscopy (XAS) coupled with electrochemical studies and density functional theory (DFT) calculations to further understand how Ni single atoms work in conjunction with the nitrogen-doped carbon matrix to promote CORR to CO, and how the presence of impurities such as those present in CO-containing waste flue gases (including NO, and CN) changes the catalyst upon reduction.

Unveiling the Fluorination Pathway of Ruddlesden-Popper Oxyfluorides: A Comprehensive X-ray and Neutron Diffraction Study.

Ruddlesden-Popper oxyfluorides exhibit unique properties, but their synthesis is often hindered by low thermodynamic stability. To overcome this challenge, understanding the formation mechanism of these materials is crucial for optimizing the reaction conditions and accessing new products. This study presents an in-depth investigation of the fluorination reaction of LaNiO with poly(vinylidene fluoride) (PVDF), targeting the oxyfluorides LaNiOF and LaNiOF, which exhibit distinct structural distortions.

Enhanced methanogenesis of wastewater anaerobic digestion by nanoscale zero-valent iron: Mechanism on intracellular energy conservation and amino acid metabolism.

Nanoscale zero-valent iron (nZVI)-mediated anaerobic digestion commonly focuses on electron transfer between syntrophic bacteria, neglecting intracellular energy conservation strategies and amino acid metabolism. In this study, FH dehydrogenase abundance increased by 5.1 %, 27.

Coral-Inspired Zinc Acrylate Polymer Utilizing Coumarin as the Fluorescent Unit for Marine Antifouling.

The need for low-cost and effective antifouling solutions drives innovation in the fields of chemistry, materials science, and biology. In this work, guided by the antifouling strategies used by fluorescent corals, a series of fluorescent zinc acrylate polymer coatings containing coumarin units (ZAR-coumarin) was successfully prepared. The ZAR-coumarin coatings demonstrated excellent antifouling properties due to the synergistic action of multiple antifouling mechanisms, including fluorescent antifouling, natural bactericidal activity, and self-polishing surface renewal (due to ester group (-COO-Zn-OOC-) cleavage).