Antibodies in nuclear medicine.

Radiolabeled antibodies in nuclear medicine may be used for therapy or diagnosis. Diagnostic antibodies may be monoclonal antibodies (mAbs), directed against a specific disease, or polyclonal antibodies, which are used largely as intravascular reference markers or markers of vascular permeability in the imaging of inflammatory disease.

Regulation of calcium-activated nonselective cation channel activity by cyclic nucleotides in the rat insulinoma cell line, CRI-G1.

The regulation of a calcium-activated nonselective cation (Ca-NS+) channel by analogues of cyclic AMP has been investigated in the rat insulinoma cell line, CRI-G1. The activity of the channel is modulated by cyclic AMP in a complex way. In the majority of patches (83%) tested concentrations of cyclic AMP of 10 microM and above cause an inhibition of channel activity which is immediately reversible on washing.

The effects of pyridine nucleotides on the activity of a calcium-activated nonselective cation channel in the rat insulinoma cell line, CRI-G1.

The activity of a calcium-activated nonselective (Ca-NS+) channel in a rat insulinoma cell line (CRI-G1) is inhibited by pyridine nucleotides in excised patches. The effects of all four pyridine nucleotides tested, beta-NAD+, beta-NADH, beta-NADP+ and beta-NADPH were very similar when tested at 0.1 mM, and at 1 mM the phosphorylated forms, beta-NADP+ and beta-NADPH, appeared to be slightly more potent than beta-NAD+ and beta-NADH.

Nucleotide Regulation of a calcium-activated cation channel in the rat insulinoma cell line, CRI-G1.

The nucleotide regulation of a calcium-activated nonselective cation (Ca-NS+) channel has been investigated in the rat insulinoma cell line CRI-G1. The activity of the channel is reduced by both AMP and ADP (1-100 microM) in a concentration-dependent manner, with AMP being more potent than ADP. At lower concentrations (0.

An in vitro profile of activity for the (+) and (-) enantiomers of spiradoline and PD117302.

Kappa- and mu-opioid binding site affinities of the kappa-selective ligand U62066 and its optical isomers, (+)-U63639 and (-)-U63640 were compared with those of the structurally related ligand PD117302 and its respective isomers, (+)-PD123497 and (-)-PD123475. The relative efficacies of each compound were also established using the guinea-pig ileum, rat and rabbit vas deferens smooth muscle bioassays. The specific opioid receptor mediating the agonist behaviour was determined in the guinea-pig ileum bioassay by obtaining pKB values for naloxone and for the kappa-selective antagonist nor-binaltorphimine.

The surgical management of dysthyroid related eyelid retraction using Mersilene mesh.

The use of Mersilene mesh as a spacer material in the surgical management of seven patients with dysthyroid-related eyelid retraction is presented. To date, fourteen eyelids have been operated on using this material with an average follow-up of nine months. It appears that Mersilene mesh is a realistic alternative to preserved sclera in the surgical management of such cases.

Effects of cholecystokinin and pentagastrin on rat hippocampal neurones maintained in vitro.

Extracellular and intracellular recordings from CA1 neurones of rat hippocampal slices were undertaken to assess the relative potencies of cholecystokinin fragments. The CCK peptides displayed a large variability in their effects on extracellularly recorded population spikes. Intracellular recordings from CA1 neurones revealed a more consistent excitant action of these compounds.

Monoclonal antibodies for organ transplantation: prospects for the future.

Monoclonal antibodies (MoAbs) have the potential to facilitate the induction of transplantation tolerance. Realization of this potential requires both a better understanding of the cellular events concerned with rejection and tolerance, and a more detailed approach to improving antibodies for therapy. This report reviews work in our laboratory directed toward these goals.

CCK 8 analgesia and hyperalgesia after intrathecal administration in the rat: comparison with CCK-related peptides.

The C-terminal octapeptide of cholecystokinin (CCK 8) was administered intrathecally to rats. Doses in the nanogram range produced weak but significant antinociception in the paw pressure test five minutes after injection whereas microgram doses of CCK 8 produced hyperalgesia. The CCK 8-induced analgesia or hyperalgesia was not seen in the tail flick test and was not associated with motor incapacitation or any other noticeable side effects.