Autophosphorylation of the Tousled-like kinases TLK1 and TLK2 regulates recruitment to damaged chromatin via PCNA interaction.

Tousled-like kinases 1 and 2 (TLK1 and 2) are cell cycle-regulated serine/threonine kinases that are involved in multiple biological processes. Mutation of TLK1 and 2 confer neurodegenerative diseases. Recent studies demonstrate that TLK1 and 2 are involved in DNA repair.

State Variation in Uptake of Pharmacy-Based CLIA-Waived Testing: Impact of Laws and Regulations.

Community pharmacies have grown to be an increasingly important provider of CLIA-waived tests, just second to physician offices as the venue with the most waivers. Yet, individual variation is still observed across states with respect to the percentage of pharmacies holding a CLIA-waiver, with a reported range of 10.7% in Massachusetts to 87.

Developing a novel dosiomics model to predict treatment failures following lung stereotactic body radiation therapy.

Purpose: The purpose of this study was to investigate the dosiomics features of the interplay between CT density and dose distribution in lung SBRT plans, and to develop a model to predict treatment failure following lung SBRT treatment.

Methods: A retrospective study was conducted involving 179 lung cancer patients treated with SBRT at the University of Nebraska Medical Center (UNMC) between October 2007 and June 2022. Features from the CT image, Biological Effective Dose (BED) and five interaction matrices between CT and BED were extracted using radiomics mathematics.

Skin Cancer Awareness, Beliefs, Behaviors, and Access to Care Among the Homeless and Underinsured Patients Using a Free Clinic in Nebraska.

Introduction: To understand the attitudes, beliefs, knowledge, and access to care surrounding sun safety for a primarily homeless or underinsured patient population at a student-run health clinic.

Methods: All adult attendees at the health clinic were invited to complete an anonymous 16-item questionnaire that assessed their sun safety history, practices, knowledge, and beliefs.

Results: Fifty participants completed our questionnaire, with 35 individuals (70%) reporting that they were without permanent residence, and 21 individuals indicating that they were uninsured or using Medicaid (42%).

Determining the Impact of the Reversal of Roe v. Wade on Birth Control and Infertility Trends Using Google Trends.

Introduction Overturning Roe v. Wade left many concerned about birth control options and future fertility. This study aims to report Google (Google, Inc.

Tobacco and cannabis co-use by sexual minority adults in the United States, 2022.

Introduction: Given the negative health outcomes of tobacco and cannabis co-use, understanding the co-use of tobacco and cannabis is important, particularly regarding those with higher health burdens, such as sexual minority individuals. This study examined the co-use of tobacco and cannabis by sexual identity.

Methods: The adult sample from the 2022 National Survey on Drug Use and Health (NSDUH) was used for this study.

Porcine peritoneal macrophages are susceptible to porcine reproductive and respiratory syndrome virus infection.

Previous studies have suggested that porcine peritoneal macrophages (PPMs) are resistant to PRRSV infection, whereas porcine alveolar macrophages (PAMs) are highly susceptible. This contrast is intriguing, as both cell types belong to the same monocyte/macrophage family. The current study aimed to investigate the host factors contributing to the differing susceptibility of PPMs and PAMs to PRRSV infection.

Telemedicine: Future of the healthcare system and its impact on patient satisfaction: A literature review.

Background And Objectives: The utilization of telemedicine has increased dramatically since the onset of the COVID-19 pandemic. In this review, we examined studies published within the past five years that investigated the impact of telemedicine on patient satisfaction.

Methods: Four investigators utilized PubMed and Google Scholar to find studies published within the past five years that assessed patient satisfaction with telemedicine in the field of adult primary care, using either the Press Ganey or CAHPS surveys.

Factors Affecting Vancomycin Trough Concentration; a Population Pharmacokinetic Model in Non-Critical Care Saudi Patients.

Background And Objective: Vancomycin is commonly prescribed in treatment of methicillin-resistant Staphylococcus aureus infections. While, vancomycins' pharmacokinetic vary among older patients, there is a paucity of data regarding specific characteristics influencing pharmacokinetics in Saudi adult patients. This study aims to establish a population-pharmacokinetic (Pop-PK) model for vancomycin in patients admitted to medical wards, with the focus on identification of patient characteristics influencing vancomycin trough concentrations.

THE ALCOHOL EXPOSOME.

Science is now in a new era of exposome research that strives to build a more all-inclusive, panoramic view in the quest for answers; this is especially true in the field of toxicology. Alcohol exposure researchers have been examining the multivariate co-exposures that may either exacerbate or initiate alcohol-related tissue/organ injuries. This manuscript presents selected key variables that represent the Alcohol Exposome.

Gridded drought response assessment of winter wheat in Oklahoma using big data and AquaCrop-OS.

Winter wheat is the most dominant crop in Oklahoma and critically important to the economy of agricultural industry in this state and the region. However, weather anomalies such as droughts, which are frequent in Oklahoma, pose serious threats to winter wheat yield. This study was conducted to assess the effects of droughts on the simulated yield of dryland winter wheat (Y) in Oklahoma employing a gridded approach with the AquaCrop Open Source (AquaCrop-OS).

Aquatic occurrence, fate and potential ecotoxicity of insecticide and fungicide residues originating from a biofuels production facility using pesticide-treated seeds.

Little is known about the potential impact of point source contamination from seed treatment pesticide residues and degradation products in waste products in treated seed. The presence of these pesticides and their degradation products in the environment has been associated with toxic effects on non-target organisms including bees, aquatic organisms and humans. In this study, we investigated the occurrence of twenty-two pesticide residues and their degradation products in two streams receiving runoff from land-applied wet cake, applied and spilled wastewater originating at a biofuels production facility using pesticide-treated seed as a feedstock.

Physical Activity and Sedentary Behavior in Patients With Breast Cancer: Results of a Single Cohort in the Midwestern United States.

Physical activity and sedentary behavior have significant prognostic importance in survivors of breast cancer. However, not much is known about physical activity and sedentary behavior in the midwestern United States for patients who have breast cancer. This study provides patient-reported physical activity and sedentary behavior from a cohort of patients from the midwestern United States.

Genetic vulnerability and adverse mental health outcomes following mild traumatic brain injury: a meta-analysis of CENTER-TBI and TRACK-TBI cohorts.

Background: Post-traumatic stress disorder (PTSD) and depression are common after mild traumatic brain injury (mTBI), but their biological drivers are uncertain. We therefore explored whether polygenic risk scores (PRS) derived for PTSD and major depressive disorder (MDD) are associated with the development of cognate TBI-related phenotypes.

Methods: Meta-analyses were conducted using data from two multicenter, prospective observational cohort studies of patients with mTBI: the CENTER-TBI study (ClinicalTrials.

Enoki-Inspired Microfibers and Extracellular Matrix Enhance Biaxially Interlocking Interfaces.

Taking inspiration from diverse interlocking and adhesion structures found in nature, a biaxially interlocking interface is developed in this work. This interface is formed by interconnecting two electrostatically flocked substrates and its mechanical strength is enhanced through the incorporation of enoki mushroom-shaped microfibers and deposited extracellular matrix (ECM). Tips of flocked straight fibers can be transformed into mushroom shapes through thermal treatment.

The feasibility of utilizing the open dynamic interaction network (ODIN) app to assess rEMA data across 30 days among those recovering from alcohol use disorders.

Preliminary data from a prospective micro-longitudinal study (30 days) that examined the co-evolution of return to use risk among people diagnosed with an alcohol use disorder (AUD) in residential substance treatment is presented. Data assessed the feasibility of using the open dynamic interaction network (ODIN) responsive ecological momentary assessment (rEMA). rEMA collected daily estimates on affect, urges, sober-support engagement, and use.

Case Reports: Chemokine and Cytokine Profiling in Patients with Herpetic Uveitis.

Purpose: To report cytokine/chemokine profiles of ocular fluid in two patients with herpetic uveitis.

Methods: Cytokine and chemokine profiling of ocular fluid was performed in two patients with herpetic uveitis. Ocular fluid findings were correlated with disease manifestations and the patients' clinical course.

Chiral effects at the metal center in Fe(III) spin crossover coordination salts.

Evidence of chirality was observed at the Fe metal center in Fe(III) spin crossover coordination salts [Fe(qsal)][Ni(dmit)] and [Fe(qsal)](TCNQ)from x-ray absorption (XAS) spectroscopy at the Fe 2pcore threshold. Based on the circularly polarized XAS data, the x-ray natural circular dichroism for [Fe(qsal)][Ni(dmit)] and [Fe(qsal)](TCNQ)is far stronger than seen for [Fe(qsal)]Cl suggesting this natural circular dichroism signature is a ligand effect rather than a result of just a loss of octahedral symmetry on the Fe core. The larger the chiral effects in the Fe 2p core to bound XAS, the greater the perturbation of the Fe 2pto 2pspin-orbit splitting seen in the XAS spectra.

Vertebral Body Tethering in Skeletally Immature Patients: Results of a Prospective U.S. FDA Investigational Device Exemption Study.

Background: The purpose of this study was to report on 2-year results of vertebral body tethering (VBT), performed under a Food and Drug Administration protocol, to obtain insight into outcomes and complications.

Methods: Forty prospectively enrolled patients with adolescent idiopathic scoliosis (AIS) who had a Sanders score of ≤4 or a Risser score of ≤2 underwent VBT for curves between 40° and 70°. Surgical, radiographic, and patient-reported outcomes were reviewed at a minimum 2-year follow-up.

Novel antibiotic resistance profiles in bacteria isolated from oil fly larvae Helaeomyia petrolei living in the La Brea Tar Pits.

Larvae from the petroleum oil fly, Helaeomyia petrolei, live in the asphaltene and polyaromatic hydrocarbon rich asphalt seeps of Rancho La Brea, Los Angeles, California. These larvae pass high amounts of viscous asphalt through their digestive system, and their gut microbiota is exposed to these extreme conditions. Environmental stress response mechanisms can co-select for antibiotic resistance, and in the current study we used 16S rRNA and genomic sequencing along with the Comprehensive Antibiotic Resistance Database (CARD) tools to characterize antibiotic resistance profiles from six bacteria previously isolated from the oil fly larval intestinal tract, linking phenotypic and genotypic resistance profiles.

Prediction of Tidal Volume in Newborns Through a Novel Three-Dimensional Model: A Viability Study.

Introduction: Although noninvasive monitoring through quantifying rib cage movement has been useful in serial assessment of pulmonary function in newborns, measuring tidal volume (TV) is commonly performed invasively. As it is the most basic measure of pulmonary function, expanding its assessment to a noninvasive measure can contribute to clinical findings and interpretations in neonatal clinical practice.

Objective: (1) Create a noninvasive measurement tool for TV for neonatal clinical use; (2) Evaluate the agreement between measured TV and predicted TV.

Latitudinal Clines in an Ectothermic Vertebrate: Patterns in Body Size, Growth Rate, and Reproductive Effort Suggest Countergradient Responses in the Prairie Lizard.

Although we have evidence that many organisms are exhibiting declines in body size in response to climate warming, we have little knowledge of underlying mechanisms or how associated phenotypic suites may coevolve. The better we understand coadaptations among physiology, morphology, and life history, the more accurate our predictions will be of organismal response to changing thermal environments. This is especially salient for ectotherms because they comprise 99% of species worldwide and are key to functioning ecosystems.

Hyperglycemia Inhibits AAA Expansion: Examining the Role of Lysyl Oxidase.

Abdominal aortic aneurysm (AAA) is a common, progressive and potentially fatal dilation of the most distal aortic segment. Multiple studies with longitudinal follow-up of AAA have identified markedly slower progression among patients affected with diabetes. Understanding the molecular pathway responsible for the growth inhibition could have implications for therapy in nondiabetic AAA patients.