128 results match your criteria: "National Veterinary and Food Research Institute[Affiliation]"
Mycotoxin Res
June 2000
National Veterinary and Food Research Institute (EELA), Department of Chemistry, P.O. Box 45 (Hämeentie 57), FIN-00581, Helsinki, Finland.
A gas chromatographic-electron capture detection (GC-ECD) method for the analysis of deoxynivalenol (DON) in cereals was investigated. The sample was extracted with a mixture of acetonitrile-water and purified with a MycoSep #225 column. The silylation was performed with Tri-Sil-TBT reagent, followed by dilution with hexane and a washing step with buffer.
View Article and Find Full Text PDFJ Appl Microbiol
May 2000
Department of Food Microbiology, National Veterinary and Food Research Institute, Research and Development Centre, Valio Ltd, Department of Mathematics, University of Helsinki, Finland.
In comparison with standard methods, enrichment in half-Fraser broth for 24 h at 30 degrees C, followed by plating out onto Listeria monocytogenes blood agar (LMBA) and PALCAM medium combined with an additional streak proved to be the most rapid and specific method for the detection of indigenous L. monocytogenes populations from soft mould-ripened cheese. This procedure, with a high sensitivity (93%) and a low detection limit (1-10 cfu 25 g-1), provided negative and presumptive positive results within 2-3 d.
View Article and Find Full Text PDFBr Poult Sci
December 1999
National Veterinary and Food Research Institute, Helsinki, Finland.
1. Newly-hatched broiler chicks were treated orally with a commercial competitive exclusion product (Broilact) in 3 replicate trials 2. After 24 h the treated chicks and untreated control chicks were challenged orally with approximately 10(4) cfu of Campylobacter jejuni.
View Article and Find Full Text PDFFood Addit Contam
August 1999
Department of Food Microbiology, National Veterinary and Food Research Institute, Helsinki, Finland. anna-liisa
Microbiological and chemical identification of antimicrobial drug residues was attempted in 95 kidney and 76 muscle samples from 58 cattle, 36 pigs and one horse which had revealed kidneys positive to an inhibitor test. Information on pre-slaughter medication with one antimicrobial drug was available for 63% of the carcasses. Microbiological identification was performed by agar diffusion using 17 or 18 combinations of eight test bacteria, varying medium pH and three substances blocking the action of certain antimicrobials.
View Article and Find Full Text PDFActa Vet Scand
July 2001
National Veterinary and Food Research Institute, Regional Laboratory in Seinäjoki, Germany.
In a survey in Finland in 1995, 14,919 colostral whey samples from 530 farrowing herds were analysed by a monoclonal blocking-ELISA to detect antibodies to Mycoplasma hyopneumoniae (M. hyopneumoniae). Antibodies were detected in 274 (1.
View Article and Find Full Text PDFJ Gen Virol
October 1999
Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK6.
The evolution of rabies viruses of predominantly European origin was studied by comparing nucleotide sequences of the nucleoprotein and glycoprotein genes, and by typing isolates using RFLP. Phylogenetic analysis of the gene sequence data revealed a number of distinct groups, each associated with a particular geographical area. Such a pattern suggests that rabies virus has spread westwards and southwards across Europe during this century, but that physical barriers such as the Vistula river in Poland have enabled localized evolution.
View Article and Find Full Text PDFDis Aquat Organ
September 1999
National Veterinary and Food Research Institute, Oulu Regional Laboratory, Finland.
Two practical methods are reported for treating feral Baltic salmon with thiamine hydrochloride against M74 syndrome (abnormally high yolk-sac fry mortality of the Baltic salmon). Both bathing of the yolk-sac fry in thiamine hydrochloride (1000 mg l-1, 1 h) and a single intraperitoneal injection given to the female brood fish (100 mg kg-1 fish) during the summer 3 mo before stripping were shown to elevate the whole body total thiamine concentration in the fry. Both treatments were also shown to be effective in preventing mortality due to M74 syndrome.
View Article and Find Full Text PDFEpidemiol Infect
June 1999
National Veterinary and Food Research Institute, Kuopio Regional Laboratory, Finland.
Salmonella enterica serovar Infantis is endemic in Finnish cattle. Feed contaminated with S. Infantis was distributed to cattle farms in May 1995.
View Article and Find Full Text PDFInt J Food Microbiol
March 1999
National Veterinary and Food Research Institute, Department of Food Microbiology, Helsinki, Finland.
One hundred and ten samples of ready-to-eat, vacuum-packed, smoked and cold-salted fish products were collected from retail outlets in southern Finland during 1996 for examination of the occurrence and level of Listeria monocytogenes. The samples originated from 12 producers. Positive samples with levels exceeding 100 CFU/g were encountered mainly in one of the producers (no.
View Article and Find Full Text PDFVet Microbiol
March 1999
National Veterinary and Food Research Institute, Department of Virology and Epidemiology, Helsinki, Finland.
A survey for antibodies to maedi-visna virus (MV) in the Finnish sheep surveillance flocks was conducted in 1994. Examination of a total of 12931 serum samples from animals over 1 year of age from 545 flocks (81% of all flocks) revealed eight seropositive flocks and the subsequent epidemiological investigation yielded one additional seropositive flock, indicating a low prevalence of 1.6%.
View Article and Find Full Text PDFPrev Vet Med
March 1999
Department of Virology and Epidemiology, National Veterinary and Food Research Institute, Helsinki, Finland.
The objectives were to investigate the association between postcolostral serum IgG and the development of calf pneumonia, to elaborate the risk factors for pneumonia, and to compare the risk factors for clinician- and caregiver-diagnosed respiratory disease. In a prospective observational cohort study, 410 heifer calves were clinically examined weekly by a veterinarian during their first 3 months of life. With an initial questionnaire and during these weekly visits, management data were collected.
View Article and Find Full Text PDFVet Microbiol
January 1999
National Veterinary and Food Research Institute, Helsinki, Finland.
Bulk milk samples from every herd supplying milk to dairies in Finland were examined for the presence of antibodies to BVD virus (BVDV) annually during 1993-1997. The highest prevalence, 0.99% in 1994, declined to 0.
View Article and Find Full Text PDFActa Vet Scand
February 1999
National Veterinary and Food Research Institute, Regional Laboratory Oulu, Finland.
During the winter 1991-92, 42 reindeer hinds of the Kaamanen Experimental Reindeer Herd in Finnish Lapland, naturally infected with various parasites, were allocated to 3 groups. One group was an untreated control group and the other 2 groups received either moxidectin or ivermectin at a dose of 200 micrograms kg-1 subcutaneously. The efficacy of treatment was followed with monthly faecal examinations for nematode eggs and counting of warbles, Hypoderma tarandi larvae, and throat bots, Cephenemyia trompe larvae, from live animals in spring.
View Article and Find Full Text PDFActa Vet Scand
November 1998
National Veterinary and Food Research Institute, Regional Laboratory, Seinäjoki, Finland.
Swine enzootic pneumonia causes heavy economical losses in fattening herds. The aim of this study was to get an estimate of the prevalence of Mycoplasma hyopneumoniae in the province of Vaasa in Western Finland. There were 112 farrowing herds randomly chosen for a survey.
View Article and Find Full Text PDFAdv Exp Med Biol
January 1999
Virology Unit, National Veterinary and Food Research Institute, Helsinki, Finland.
A serological study for antibodies against equine arteritis virus (EAV) in Finland was performed during 1996. All equine sera delivered to the Virology Unit at the National Veterinary and Food Research Institute were tested with a micro-neutralization test, using the Arvac strain as antigen. The study also included imported horses to evaluate EAV circulation in the countries of origin.
View Article and Find Full Text PDFJ Appl Microbiol
July 1998
National Veterinary and Food Research Institute, Helsinki, Finland.
The survival of Yersinia enterocolitica serotype O:3 was examined in an in vitro model of the porcine ileum in the presence of normal ileal microflora using a medium supplemented with the growth-promoting antimicrobials avoparcin, spiramycin, tylosin, carbadox and olaquindox, or without any antimicrobials, at 39 degrees C for 5 d. Growth of Y. enterocolitica was inhibited in all trials in the following order: avoparcin < carbadox < spiramycin < no feed additives < tylosin < olaquindox.
View Article and Find Full Text PDFDis Aquat Organ
April 1998
National Veterinary and Food Research Institute, Department of Virology and Epidemiology, Helsinki, Finland.
We have isolated a large virus from pike-perch Stizostedion lucioperca fingerlings with no signs of disease. The biochemical structural, and serological properties of this newly isolated virus suggest that it belongs to the family Iridoviridae. The virus multiplied and was cytopathogenic in several cultured fish cell lines.
View Article and Find Full Text PDFFood Addit Contam
April 1998
National Veterinary and Food Research Institute, Department of Chemistry, Helsinki, Finland.
A high-performance liquid chromatographic-atmospheric pressure chemical ionization mass spectrometric method was developed for the determination of volatile nitrosamines in dry sausages. Tandem mass spectrometry was applied for the detection of N-nitrosopyrrolidine, N-nitrosodiethylamine and N-nitrosopiperidine. N-nitrosomethylamine was detected by using the selected ion monitoring mode.
View Article and Find Full Text PDFJ Helminthol
March 1998
National Veterinary and Food Research Institute, Regional Laboratory, Oulu, Finland.
Three hundred and twenty seven European lynx (Lynx lynx) were examined for Trichinella sp. larvae using the muscle digestion method. The animals were of both sexes, various ages, and collected between 1989 and 1994 in different locations in Finland.
View Article and Find Full Text PDFInt J Food Microbiol
March 1998
National Veterinary and Food Research Institute, Department of Food Microbiology, Helsinki, Finland.
Listeria monocytogenes blood agar (LMBA) was compared to Listeria selective agar based on lithium chloride and ceftazidime (LA) and to the Oxford and Palcam media recommended by ISO and IDF for the detection and enumeration of L. monocytogenes from foodstuffs and food-processing environments. LMBA is based on trypticase soy agar with the following additions: sheep blood (5%) and as selective agents lithium chloride (10 g/l), polymyxin B sulphate (10 mg/l) and ceftazidime (20 mg/l), whereas the selectivity of LA is based on lithium chloride (15 g/l) and ceftazidime (15 g/l).
View Article and Find Full Text PDFActa Vet Scand
June 1998
National Veterinary and Food Research Institute, University of Helsinki, Finland.
Two surveys were carried out (during 1988 and 1995) to estimate the prevalence of bovine mastitis in Finland. In 1988, 17,111 quarter milk samples were obtained from 4495 cows, and in 1995 the corresponding figures were 10,410 and 2648. Antimicrobial susceptibility of mastitis pathogens was studied.
View Article and Find Full Text PDFThe virus isolation-immunoperoxidase test (IPX) on cell cultures and the reverse transcription-polymerase chain reaction (RT-PCR) assay were compared for the detection of bovine viral diarrhoea virus (BVDV) directly in serum samples. Material for this study consisted of 403 sera originating from cattle in 41 BVDV-infected Finnish dairy herds and one suckler cow herd. The presence of virus was demonstrated in 48 samples by both assays.
View Article and Find Full Text PDFVet Rec
October 1997
Department of Virology and Epidemiology, National Veterinary and Food Research Institute, Helsinki, Finland.
Canine distemper reappeared in dogs in Finland in 1990 after a 16-year absence. In 1994 to 1995 an outbreak occurred in areas with a high density dog population which involved dogs vaccinated against distemper. The estimated total number of cases was at least 5000, and 865 cases were confirmed by indirect fluorescent antibody testing of 3649 epithelial cell samples.
View Article and Find Full Text PDFStaphylococcus aureus isolates (N = 40) from bovine mastitis were characterized by random amplified polymorphic DNA-PCR (RAPD-PCR), ribotyping and biotyping. The isolates were collected in the veterinary surveillance area of the Ambulatory Clinic, Faculty of Veterinary Medicine, University of Helsinki from 20 quarters during the acute phase of infection and from the same quarters 3 weeks after cessation of therapy. The aim of the study was to compare the S.
View Article and Find Full Text PDFJ Vet Diagn Invest
October 1996
National Veterinary and Food Research Institute, Departments of Virology, Helsinki, Finland.
Toxigenic Pasteurella multocida is the causative agent of progressive atrophic rhinitis (PAR), a serious respiratory infection of swine. Diagnosis of the disease has hitherto been based on clinical signs, pathologic findings, and subsequent isolation of the agent. The best Finnish pig breeding herds participating in the Finnish Pig Health Scheme have been surveyed for PAR since 1963, and the disease has been eradicated from these herds.
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