Profiling of snoRNAs in Exosomes Secreted from Cells Infected with Influenza A Virus.

Small nucleolar RNAs (snoRNAs) are non-coding RNAs (ncRNAs) that regulate many cellular processes. Changes in the profiles of cellular ncRNAs and those secreted in exosomes are observed during viral infection. In our study, we analysed differences in expression profiles of snoRNAs isolated from exosomes of influenza (IAV)-infected and non-infected MDCK cells using high-throughput sequencing.

Immunoinformatics design of a novel multiepitope vaccine candidate against non-typhoidal salmonellosis caused by Salmonella Kentucky using outer membrane proteins A, C, and F.

The global public health risk posed by Salmonella Kentucky (S. Kentucky) is rising, particularly due to the dissemination of antimicrobial resistance genes in human and animal populations. This serovar, widespread in Africa, has emerged as a notable cause of non-typhoidal gastroenteritis in humans.

Detection of Echinococcus spp. and other taeniid species in lettuces and berries: Two international multicenter studies from the MEmE project.

Cystic and alveolar echinococcosis are severe zoonotic diseases characterized by long asymptomatic periods lasting months or years. Viable Echinococcus spp. eggs released into the environment through the feces of canids can infect humans through accidental ingestion via hand-to-mouth contact or consumption of contaminated food or water.

The grey wolf as a host of . and other helminths - a new zoonotic threat in Poland.

Introduction: The aim of this study was to estimate the occurrence of spp. and other helminth infections in grey wolves in south-eastern Poland.

Material And Methods: Overall, 74 samples of wolf faeces were examined with a multiplex PCR and a system of real-time quantitative PCR methods to detect and identify spp.

High-throughput sequencing as a potential tool in the quality control of infectious bronchitis vaccines.

Introduction: In Europe, veterinary vaccines are strictly controlled by the Official Medicines Control Laboratories (OMCLs) of the General European OMCL Network, coordinated by the European Directorate for the Quality of Medicines & HealthCare. Despite a meticulous verification programme for immunological veterinary medicinal products (IVMPs), the products' genomic composition has not yet been subject to evaluation in veterinary pharmacy.

Material And Methods: A study was carried out on Poland's poultry vaccines containing the infectious bronchitis virus which have the greatest market penetration.

Determination of clostebol residues in the urine of slaughter animals using liquid chromatography-tandem mass spectrometry.

Introduction: Synthetic anabolic hormones, which may pose a potential risk to human health, should not be used in fattening food-producing animals. Because of the hormonal effects they cause, growth-promoting compounds are banned by legislation in the EU for use in animal husbandry. Consequently, all EU member states are required to conduct monitoring tests on the content and residues of these compounds in prescribed biological matrices to ensure the safety of food consumers.

First isolation of from clinical specimens collected on a pig farm in Poland.

Introduction: Successful retrieval of from porcine clinical specimens has been rarely described, and data has only been obtained from a few swine-producing countries. Therefore, the aim of this study was the isolation of recovered from a specimen originating from a commercial pig farm located in Poland.

Material And Methods: Seven dead 12-week-old pigs weighing 24-26 kg with joint swelling of the hind legs were selected on a modern farrow-to-nursery farm in Poland in October 2023.

Fatal and sp. co-infestation in wolves () at the Białowieża National Park, Poland - is it a consequence of climate change?

Introduction: In winter 2021/2022, a wolf population in the primeval Białowieża Forest in Poland was struck by an outbreak of severe mange caused by mixed infestations of and mites. We present an epidemiological analysis of this mange which caused significant morbidity and mortality.

Material And Methods: Ten sites known for wolf activity were monitored by camera trapping.

Serological evidence of antibodies in wild boars () in Poland.

Introduction: This study aimed to evaluate the seroprevalence of among wild boars inhabiting Poland.

Material And Methods: Serum samples were collected from 281 wild boars of varying ages (juveniles, adolescents and adults) and subjected to testing using a commercial indirect ELISA assay.

Results: The prevalence of antibodies was notably high, reaching 75.

First molecular characterisation of in a pig carcass condemned during routine meat inspection in Poland.

Introduction: This article presents the fourth detection of macroscopic cystic lesions due to sarcocystosis in domestic pigs during routine meat inspection worldwide, and the first molecular detection of in a domestic pig in Poland. Pigs can become intermediate hosts for by accidental ingestion of oocysts or sporocysts present in food or water contaminated by the faeces of canids (definitive hosts).

Material And Methods: The affected swine showed no clinical symptoms such as weight loss, dermatitis or dyspnoea suggesting sarcocystosis.

Prevalence of chlamydiae in dairy cattle herds and factors contributing to the spread of infections.

Introduction: Different species affect cattle and contribute to economic losses. One of them, , is a globally endemic livestock pathogen. Despite its endemicity, prevalence data from Poland have so far been limited.

in Sewage Sludge and Digestate Intended for Soil Fertilization.

Substances of organic origin are seeing increasing use in agriculture as rich sources of nutrients for plants. The aim of this study was to determine the microbiological contamination of sewage sludge and digestate to assess their safe use as fertilizers in Poland. The assessment of microbial soil, sewage sludge and digestate contamination was based on the total number of mesophilic bacteria and Gram-negative bacteria from the family.

The usefulness of botulinum toxin injections in oncology - Clinical and laboratory perspectives.

The use of botulinum toxins in various oncological conditions brings long-term results in the treatment of chronic pain without burdening internal organs, as is the case with the use of opioids. Numerous reports indicate a possible apoptotic effect achieved in vitro and in vivo. Most literature data suggest that using botulinum toxins to support oncological treatment gives positive results, and in most cases, it is free of side effects.

Commercial Day-Old Chicks in Nigeria Are Potential Reservoirs of Colistin- and Tigecycline-Resistant Potentially Pathogenic .

Frequent use of colistin (COL) and tetracyclines in the Nigerian poultry sector potentially triggers bacterial resistance against COL and tigecycline (TIG), which are last-line antibiotics used to treat multidrug-resistant infections. This study aimed to isolate COL- and TIG-resistant . from commercial day-old chicks distributed to poultry farmers in Nsukka Southeastern Nigeria, assess the production of extended-spectrum β-lactamase (ESBL) and carbapenemase by the isolates, and establish their pathogenic potentials.

Isolation and Characterization of a Novel Jumbo Phage HPP-Temi Infecting Pa9 and Increasing Host Sensitivity to Ciprofloxacin.

is a bacteria responsible for many hospital-acquired infections. Phages are promising alternatives for treating infections, which are often intrinsically resistant. The combination of phage and antibiotics in clearing bacterial infection holds promise due to increasing reports of enhanced effectiveness when both are used together.

Isolation and Molecular Characterization of Pathogenic Leptospira spp. from Brown Rats (Rattus norvegicus) in Zaria and Environs, Kaduna, Nigeria, 2022.

Leptospirosis caused by the pathogenic serovars of Leptospira spp. is a zoonotic disease of global importance. Brown rats (Rattus norvegicus), due to their worldwide distribution and reservoir host status, are considered the most important reservoir for environmental leptospiral persistence and infections in animals.

Isolation and molecular characterization of Shiga toxin-producing Escherichia coli (STEC) from bovine and porcine carcasses in Poland during 2019-2023 and comparison with strains from years 2014-2018.

The presence of Shiga toxin-producing Escherichia coli (STEC) on bovine and porcine carcasses during 2019-2023 was investigated. A total of 368 bovine and 87 porcine carcasses were tested using the ISO/TS 13136 standard and the STEC isolates were further characterized with whole genomic sequencing (WGS). It was found that 119 (32.

Assessing the impact of structural modifications in the construction of surveillance network for Peste des petits ruminants disease in Nigeria: The role of backbone and sentinel nodes.

Peste des petits ruminants (PPR) is a highly contagious disease affecting mainly sheep and goats. Livestock movements contribute to the spread of the disease by introducing it to naive areas or exposing susceptible animals to it in infected regions. Because of its socio-economic impact, the Food and Agriculture Organisation (FAO) and the World Organization for Animal Health (WOAH) have set the goal to eradicate it by 2030, one of the key steps being the improvement of surveillance networks.

Pathology and molecular detection of influenza A subtype H9N2 virus in commercial poultry in Nigeria, 2024.

Background: After the first Avian Influenza H5N1 outbreak in Nigerian poultry in 2006, subsequent waves of outbreaks occurred, causing substantial losses. Despite effective control measures by 2008, a resurgence in 2015 led to further losses and required depopulation efforts.

Aim: The aim of this study was to do pathology and molecular detection of influenza A subtype H9N2 virus in commercial poultry in Nigeria during 2024.

Evaluation of β-Actin and Mitochondrial DNA Levels in Determining the Age of Remains.

African Swine Fever (ASF) is an infectious disease affecting pigs and wild boars, causing significant economic losses. Epidemiological surveillance plays an important role in minimizing its impact. The aim of this study was to evaluate the usefulness of β-actin DNA and mitochondrial DNA (mtDNA) levels in determining the age of animal remains from the Suidae family, which could be helpful in epidemiological investigations.

Identification of Bacterial Pathogens in Organic Food of Animal Origin in Poland.

The consumption of organic food has increased in recent years. In organic rearing animals are exposed to outdoor conditions, which may increase their risk of infection from various pathogens. In the present study the occurrence of the most significant foodborne pathogenic bacteria in organic meat and ready-to-eat organic meat products was assessed.