Targeting Mesothelin Enhances Personalized Neoantigen Vaccine Induced Antitumor Immune Response in Orthotopic Pancreatic Cancer Mouse Models.

The immunosuppressive microenvironment in pancreatic cancer, characterized by low tumor-specific T cells and excessive fibrosis, limits the effectiveness of immunotherapy. Here, three datasets and multi-immunofluorescence staining of tissue microarrays in pancreatic cancer indicate that mesothelin (MSLN) expression negatively correlates with cytotoxic T cells in tumor. Anti-MSLN antibody (αMSLN) treatment of pancreatic cancer in vivo can significantly increase T cell infiltration.

Structural Basis and Mechanism of a Unique Haemophore in the Haem-Iron Acquisition by Riemerella anatipestifer.

Several bacterial pathogens employ haemophores to scavenge haem from host haemoprotein to obtain an iron source. However, no homologues of well-characterized haemophores are found in Riemerella anatipestifer, a bacterium belonging to the order Flavobacteriales that encodes haem uptake systems. Herein, a unique haemophore RhuH is characterized in this bacterium.

Inversion of Circularly Polarized Luminescence in the Left-Handed Chitosan-Templated Co-assemblies.

Circularly polarized luminescence (CPL) materials are attractive due to their unique applications in fields such as 3D displays, information encryption, and chiroptical switches. Natural biomolecules-based CPL materials are gaining plenty of attention due to their chiral diversity and sustainability. However, it is still challenging to construct CPL materials with opposite CPL signs from a single natural biomolecule due to its inherent chirality.

Integrating Humidity-Resistant and Colorimetric COF-on-MOF Sensors with Artificial Intelligence Assisted Data Analysis for Visualization of Volatile Organic Compounds Sensing.

Direct visualization and monitoring of volatile organic compounds (VOCs) sensing processes via portable colorimetric sensors are highly desired but challenging targets. The key challenge resides in the development of efficient sensing systems with high sensitivity, selectivity, humidity resistance, and profuse color change. Herein, a strategy is reported for the direct visualization of VOCs sensing by mimicking human olfactory function and integrating colorimetric COF-on-MOF sensors with artificial intelligence (AI)-assisted data analysis techniques.

A Dual-Pipeline Lactate Removal Strategy to Reverse Vascular Hyperpermeability for the Management of Lipopolysaccharide-Induced Sepsis.

Sepsis is an underappreciated yet severe threat to human life, marked by organ dysfunction and high mortality resulting from disordered inflammatory responses to blood infection. Unfortunately, no specific drugs are available for effective sepsis treatment. As a pivotal biomarker for sepsis, lactate levels are closely related to vascular permeability and sepsis-associated mortality.

Unveiling the Li/Electrolyte Interface Behavior for Dendrite-Free All-Solid-State Lithium Metal Batteries by Operando Nano-Focus WAXS.

Poly(ethylene oxide) (PEO)-based solid composite electrolytes suffer from poor conductivity and lithium dendrite growth, especially toward the metallic lithium metal anode. In this study, succinonitrile (SN) is incorporated into a PEO composite electrolyte to fabricate an electrode-compatible electrolyte with good electrochemical performance. The SN-doped electrolyte successfully inhibits the lithium dendrite growth and facilitates the SEI layer formation, as determined by the operando nanofocus wide-angle X-ray scattering (nWAXS), meanwhile, stably cycled over 500 h in Li/SN-PEO/Li cell.

A Long-Term Ecological Research Data Set From the Marine Genetic Monitoring Program ARMS-MBON 2018-2020.

Molecular methods such as DNA/eDNA metabarcoding have emerged as useful tools to document the biodiversity of complex communities over large spatio-temporal scales. We established an international Marine Biodiversity Observation Network (ARMS-MBON) combining standardised sampling using autonomous reef monitoring structures (ARMS) with metabarcoding for genetic monitoring of marine hard-bottom benthic communities. Here, we present the data of our first sampling campaign comprising 56 ARMS units deployed in 2018-2019 and retrieved in 2018-2020 across 15 observatories along the coasts of Europe and adjacent regions.

MOF Nanosheet Enable Accelerated Redox Kinetics and Ultralow Overpotential for Light-Assisted Li-CO Battery.

Lithium-carbon dioxide (Li-CO₂) batteries have attracted much attention due to their high energy density, low cost, and carbon sequestration. However, the sluggish conversion kinetics between CO₂ and the discharge product lithium carbonate (Li₂CO₃) have hindered their practical applications. Herein, a flower-like photosensitive metal-organic framework (FJU-115-NS) has been employed as a cathodic electrocatalyst for Li-CO₂ batteries.

MXene-Based Flexible Memory and Neuromorphic Devices.

As the age of the Internet of Things (IoTs) unfolds, along with the rapid advancement of artificial intelligence (AI), traditional von Neumann-based computing systems encounter significant challenges in handling vast amounts of data storage and processing. Bioinspired neuromorphic computing strategies offer a promising solution, characterized by features of in-memory computing, massively parallel processing, and event-driven operations. Compared to traditional rigid silicon-based devices, flexible neuromorphic devices are lightweight, thin, and highly stretchable, garnering considerable attention.

Global Research Hotspots and Trends in Advances of the Ilizarov Technique: A Bibliometric Mapping.

Objective: An updated bibliometric analysis is needed to address the lack of comprehensive understanding of Ilizarov technique's research trends and hotspots, fostering collaboration and technology adoption. CiteSpace was utilized to perform co-citation analyzes on authors, countries, institutions, journals and cited journals, authors and cited literature, along with keywords. This approach aimed to identify leaders, collaborating institutions, and research hotspots associated with the Ilizarov technique, while also predicting future development trends.

Nitrogen-Doped Carbon Dots Prepared via Microchannel Method for Visual Detection of Copper Ions.

Copper ions (Cu) play a crucial role in biological processes; however, excessive intake can result in severe health problems. Current methods for detecting copper ions are both expensive and complex. Therefore, there is a need for efficient and straightforward visual detection methods.

Experimental Realization of On-Chip Surface Acoustic Wave Metasurfaces at Sub-GHz.

Metasurfaces, consisting of subwavelength-thickness units with different wave responses, provide an innovative possible method to manipulate elastic and acoustic waves efficiently. The application of metasurfaces to manipulate on-chip surface acoustic wave (SAW) at sub-GHz frequencies requires further exploration since their wave functions are highly demanded in nanoelectromechanical systems (NEMS), sensing, communications, microfluid control and quantum processing. Here, the experimental realization of on-chip SAW metasurfaces is reported, consisting of gradient submicron niobium (Nb) rectangular pillars positioned on a 128°Y-cut lithium niobate (LiNbO) substrate that operate at hundreds of megahertz.

mA-mRNA Reader YTHDF2 Identified as a Potential Risk Gene in Autism With Disproportionate Megalencephaly.

Among autistic individuals, a subphenotype of disproportionate megalencephaly (ASD-DM) seen at three years of age is associated with co-occurring intellectual disability and poorer prognoses later in life. However, many of the genes contributing to ASD-DM have yet to be delineated. In this study, we identified additional ASD-DM candidate genes with the aim to better define the genetic etiology of this subphenotype of autism.

A Transformative Wearable Corneal Microneedle Patch for Efficient Therapy of Ocular Injury and Infection.

Ocular injury and infection are significant causes of vision impairment and blindness globally. Effective treatment is, however, challenging due to the physical barrier of the cornea, which restricts drug penetration in the eye, as well as the presence of eye injury that necessitates continuous delivery of growth factors on the ocular surface for cornea healing. Here, we introduce a transformative wearable corneal microneedle (MN) patch designed for efficient therapy of ocular injury and infection.

Interconnected Lamellar 3D Semiconductive PCP for Rechargeable Aqueous Zinc Battery Cathodes.

2D electronically conductive porous coordination polymers/metal-organic frameworks (2D EC-MOFs) of M-HHTPs (HHTP = 2,3,6,7,10,11-hexahydroxytriphenylene; M = Co, Ni, Cu, etc.) have received extensive attention due to their ease of preparation, semiconductive properties, and tunability based on the choice of metal species. However, slight shifts between layers attenuate their specific surface area and stability.

Superior Active and Durable Air Electrode for Protonic Ceramic Cells by Metal-Oxide Bond Engineering.

Protonic ceramic cells (PCCs) have been identified as promising energy conversion devices, offering flexible fuel options and reduced operating consumption at intermediate temperatures. However, the application of traditional cobalt-based perovskite air electrodes in PCCs is hindered by their insufficient durability and high coefficient of thermal expansion. In this study, a straightforward metal-oxygen bond engineering is conducted, introducing a single-phase perovskite, BaLa(FeZn)NO (BLFZN0.

A Novel Missense Mutation of the ABL1 Gene in a Child With Congenital Heart Defects and Skeletal Malformations Syndrome.

Congenital heart defects and skeletal malformations syndrome (CHDSKM) is a rare autosomal dominant genetic disorder characterized by specific clinical features, including dysmorphic facial traits, congenital heart defects, skeletal abnormalities, joint issues, and failure to thrive. The novelty of this case lies in the identification of a novel mutation in the ABL1 gene, expanding the genetic spectrum associated with this syndrome. A 5.

Unraveling the Microenvironment and the Pathogenic Axis of HIF-1α-Visfatin-Fibrosis in Autoimmune Pancreatitis Using a Single-Cell Atlas.

Autoimmune pancreatitis (AIP) is identified as a severe chronic immune-related disorder in pancreas, including two subtypes. In this study, pancreatic lesions in patients diagnosed as either type 1 AIP or type 2 AIP are examined, and these patients' peripheral blood at single-cell level. Furthermore, flow cytometry, immunofluorescence, and functional assays are performed to verify the identified cell subtypes.

Bioprinted Micro-Clots for Kinetic Analysis of Endothelial Cell-Mediated Fibrinolysis.

Vascular hypo-fibrinolysis is a historically underappreciated and understudied aspect of venous thromboembolism (VTE). This paper describes the development of a micro-clot dissolution assay for quantifying the fibrinolytic capacity of endothelial cells - a key driver of VTE development. This assay is enabled using aqueous two-phase systems (ATPS) to bioprint microscale fibrin clots over human umbilical vein endothelial cells (HUVECs).

Diel modulation of perireceptor activity influences olfactory sensitivity in diurnal and nocturnal mosquitoes.

Olfaction and diel-circadian rhythm regulate different behaviors, including host-seeking, feeding, and locomotion, in mosquitoes that are important for their capacity to transmit disease. Diel-rhythmic changes of the odorant-binding proteins (OBPs) in olfactory organs are primarily accountable for olfactory rhythmicity. To better understand the molecular rhythm regulating nocturnal and diurnal behaviors in mosquitoes, we performed a comparative RNA-sequencing study of the peripheral olfactory and brain tissues of female Anopheles culicifacies and Aedes aegypti.

Structural insights into substrate transport and drug inhibition of the human vesicular monoamine transporter 2 (VMAT2).

Vesicular monoamine transporter 2 (VMAT2) is a proton-monoamine antiporter that is widely expressed in central and peripheral neurons and plays a crucial role in loading monoamine neurotransmitters into secretory vesicles. Dysfunction of VMAT2 causes many neuropsychiatric disorders, such as depression and Parkinson's disease. Consequently, VMAT2 is a valid and important therapeutic target.

Rational Pore Design in Multivariate Metal-Organic Frameworks for CH/CH Separation.

The arrangement of pores within the framework plays a crucial role in the gas separation and adsorption of metal-organic frameworks (MOFs), determining their overall performance. In this study, the impact on gas separation efficiency is compared using two multivariate MOF (MTV-MOF) systems with controlled pore arrangements. These systems employ two types of ligands with differing bulkiness: one is the core-shell MOF composite (CSMOF), sequentially synthesized with the bulkier ligand located at the shell, and the other is the mixed-linker MOF (MLMOF), synthesized via a one-pot reaction.

Knocking out the carboxyltransferase interactor 1 (CTI1) in Chlamydomonas boosted oil content by fivefold without affecting cell growth.

The first step in chloroplast de novo fatty acid synthesis is catalysed by acetyl-CoA carboxylase (ACCase). As the rate-limiting step for this pathway, ACCase is subject to both positive and negative regulation. In this study, we identify a Chlamydomonas homologue of the plant carboxyltransferase interactor 1 (CrCTI1) and show that this protein interacts with the Chlamydomonas α-carboxyltransferase (Crα-CT) subunit of the ACCase by yeast two-hybrid protein-protein interaction assay.

Microneedles at the Forefront of Next Generation Theranostics.

Theranostics, combining therapeutic and diagnostic functions, marks a revolutionary advancement in modern medicine, with microneedle technology at its forefront. This review explores the substantial developments and multifaceted applications of microneedles, which have evolved from basic transdermal drug delivery devices to sophisticated diagnostic and therapeutic platforms. Microneedles enhance access to biomarkers via interstitial fluid, enabling real-time monitoring of physiological conditions, such as glucose and hormone levels, thus facilitating continuous health tracking.

Microneedle Array-Based Dermal Interstitial Fluid Biopsy for Cancer Diagnosis: Advances and Challenges.

Current early cancer diagnostic technologies, such as imaging, molecular tests, endoscopic techniques, and biopsies, face considerable challenges in low-and middle-income countries (LMICs) due to high costs, procedural complexity, and limited resource access. Microneedle-based liquid biopsy for skin interstitial fluid (ISF) offers a practical and minimally invasive alternative for cancer diagnosis in these settings. This review systematically examines ISF liquid biopsy methods for their effectiveness in capturing cancer biomarkers directly from the skin and assesses their potential to address diagnostic needs in low-resource environments.