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Comparison of real-time PCR and nested PCR based on the HlyA gene for the detection of Listeria monocytogenes. Application on cheese samples.
Braz J Microbiol
June 2024
Instituto de Investigación de La Cadena Láctea (IdICaL) (INTA- CONICET), Ruta 34 Km 227, Faculty of Technology and Innovation for Development, Food Sciences Area, National University of Rafaela (UNRAf), Bv. Roca 989, (2300), Rafaela, Santa Fe, Argentina.
The aim of the present study was to compare the performance of a nested polymerase chain reaction (nPCR) and a real-time PCR based on the amplification of the HlyA gene from Listeria monocytogenes using a plasmid DNA standard. Nested PCR was developed with an internal amplification control (IAC). Both techniques were validated in soft cheese samples by comparing their results with the results of the microbiological reference method ISO 11290-1:2017.
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