Correction: Development of a structure-based computational simulation to optimize the blocking efficacy of pro-antibodies.

[This corrects the article DOI: 10.1039/D1SC01748A.].

Development of a structure-based computational simulation to optimize the blocking efficacy of pro-antibodies.

The on-target toxicity of monoclonal antibodies (Abs) is mainly due to the fact that Abs cannot distinguish target antigens (Ags) expressed in disease regions from those in normal tissues during systemic administration. In order to overcome this issue, we "copied" an autologous Ab hinge as an "Ab lock" and "pasted" it on the binding site of the Ab by connecting a protease substrate and linker in between to generate a pro-Ab, which can be specifically activated in the disease region to enhance Ab selectivity and reduce side effects. Previously, we reported that 70% of pro-Abs can achieve more than 100-fold blocking ability compared to the parental Abs.