Carrageenan gel beads for echinochrome inclusion: Influence of structural features of carrageenan.

Carrageenan (CRG) and carrageenan/chitosan (CH) gel beads (CRG/CH) were prepared as a release delivery system for echinochrome A (Ech). According to spectral data, the Ech was dispersed in the polymer matrix, interacted with CRG, was not oxidised, and remained stable after encapsulation in CRG beads. Carrageenan beads containing Ech were coated with CH by layering.

Composite Zn(II) Ferrocyanide/Polyethylenimine Cryogels for Point-of-Use Selective Removal of Cs-137 Radionuclides.

The feasibility of several approaches to the fabrication of monolith composite cryogels containing transition-metal ferrocyanides for Cs ion uptake has been evaluated. Although in the series of investigated metal ion precursors (Cu(II), Zn(II), Ni(II), and Co(II)), in situ formation of the sorption active phase in polyethyleneimine (PEI) cryogel was feasible only in the case of Zn(II) ferrocyanide, this approach has shown significant advantages over the immobilization of ex situ synthesized ferrocyanide nanoparticles. Nanoparticles of the mixed ferrocyanide ZnK[Fe(CN)] formed in situ had an average size of 516 ± 146 nm and were homogeneously distributed in the monolith located at the polymer surface rather than embedded in the matrix.

First detection of Hematodinium sp. In spiny king crab Paralithodes brevipes, and new geographic areas for the parasite in tanner crab Chionoecetes bairdi, and red king crab Paralithodes camtschaticus.

A parasitic dinoflagellate of the genus Hematodinium was found off the Pacific coast of Kamchatka in three species of crabs: red king crab Paralithodes camtschaticus, tanner crab Chionoecetes bairdi, and spiny king crab Paralithodes brevipes. This is the first detection of Hematodinium in spiny king crab. The results of the genetic analysis showed that the pathogen found in P.

' Xiphinematincola pachtaicus' gen. nov., sp. nov., an endosymbiotic bacterium associated with nematode species of the genus (Nematoda, Longidoridae).

An intracellular bacterium, strain IAS, was observed to infect several species of the plant-parasitic nematode genus (, , , , and ). The bacterium could not be recovered on axenic medium. The 16S rRNA gene sequence of IAS was found to be new, being related to the family Burkholderiaceae, class Betaproteobacteria.

The First Data on the Complete Genome of a Tetrodotoxin-Producing Bacterium.

Tetrodotoxin (TTX)-producing bacteria have attracted great interest as a model system for study of the TTX biosynthetic route. Here, we report the complete genome of the TTX-producing bacterium sp. 1839.

Studies on the Structure and Properties of Membrane Phospholipase A Inclusion Bodies Formed at Low Growth Temperatures Using GFP Fusion Strategy.

The effect of cultivation temperatures (37, 26, and 18 °C) on the conformational quality of phospholipase A (PldA) in inclusion bodies (IBs) was studied using green fluorescent protein (GFP) as a folding reporter. GFP was fused to the C-terminus of PldA to form the PldA-GFP chimeric protein. It was found that the maximum level of fluorescence and expression of the chimeric protein is observed in cells grown at 18 °C, while at 37 °C no formation of fluorescently active forms of PldA-GFP occurs.

External dsRNA Downregulates Anthocyanin Biosynthesis-Related Genes and Affects Anthocyanin Accumulation in .

Exogenous application of double-stranded RNAs (dsRNAs) and small-interfering RNAs (siRNAs) to plant surfaces has emerged as a promising method for regulation of essential genes in plant pathogens and for plant disease protection. Yet, regulation of plant endogenous genes via external RNA treatments has not been sufficiently investigated. In this study, we targeted the genes of chalcone synthase (CHS), the key enzyme in the flavonoid/anthocyanin biosynthesis pathway, and two transcriptional factors, MYBL2 and ANAC032, negatively regulating anthocyanin biosynthesis in .

A new species of Metaruncina (Gastropoda: Heterobranchia: Runcinida) from Vietnam with a discussion on the so-called rudimentary radula in runcinids.

Marine heterobranch slugs of the order Runcinida comprise 61 species, most of which inhabit the Atlantic Ocean and are referred to the genus Runcina. The Runcinida of the Pacific Ocean have been much less studied to date: only 12 species, from the genera Metaruncina Baba, 1967, Rfemsia Chernyshev, 1999, Runcinida Burn, 1963, Runcinella Odhner, 1924, Pseudoilbia M. C.

New species of latest Eocene/earliest Oligocene microgastropods (Heterobranchia: Orbitestellidae and Omalogyridae) from the Gries Ranch Formation, Lewis County, Washington State, USA.

Continued sampling of the latest Eocene to earliest Oligocene Gries Ranch Formation in Lewis County, Washington State, has yielded new heterobranch microgastropod species. Orbitestella kieli sp. nov.

Schwann cell precursors generate sympathoadrenal system during zebrafish development.

The autonomic portion of the peripheral nervous system orchestrates tissue homeostasis through direct innervation of internal organs, and via release of adrenalin and noradrenalin into the blood flow. The developmental mechanisms behind the formation of autonomic neurons and chromaffin cells are not fully understood. Using genetic tracing, we discovered that a significant proportion of sympathetic neurons in zebrafish originates from Schwann cell precursors (SCPs) during a defined period of embryonic development.

Differentiation of the frog sculpin Tilesius, 1811 (Actinopterygii, Cottidae) based on mtDNA and karyotype analyses.

A molecular genetic and karyological study of the frog sculpin Tilesius, 1811 was carried out on an extensive sample from a large area of the species' range. A total of 42 specimens was sampled from the Sea of Japan, Sea of Okhotsk, and coastal waters off the southern Kuril Islands, which makes this sampling scheme the most comprehensive to date. The level of mtDNA polymorphism was found to be low.

Identification and analysis of the biological activity of the new strain of Pseudoalteromonas piscicida isolated from the hemal fluid of the bivalve Modiolus kurilensis (F. R. Bernard, 1983).

A cultivated form of bacteria (strain 2202) was isolated from the hemal fluid of the bivalve mollusk Modiolus kurilensis. Based on the set of data collected by genetic and physiological/biochemical analyses, the strain was identified as the species Pseudoalteromonas piscicida. Strain 2202 exhibits antimicrobial activity against Staphylococcus aureus, Candida albicans, and Bacillus subtilis but not against Escherichia coli and Pseudomonas aeruginosa.

Antioxidant activity of Far Eastern bivalves in their natural habitat.

The activities of the key antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GP) and glutathione reductase (GR) as well as levels of reduced glutathione (GSH) and integral antioxidant activity (IAA), were studied in the digestive glands and gills of 14 bivalve species. Species and tissue differences of the antioxidant (AO) systems of the investigated mollusks were discussed in connection with their physiological and biochemical peculiarities. This article describes the role of the AO system of mollusks in adaptation to natural habitat conditions and shows the relationship of AO activity with the maximum habitat depth (MHD) and maximum lifespan (MLS) of these species.

A Confocal Microscopic Study of Gene Transfer into the Mesencephalic Tegmentum of Juvenile Chum Salmon, , Using Mouse Adeno-Associated Viral Vectors.

To date, data on the presence of adenoviral receptors in fish are very limited. In the present work, we used mouse recombinant adeno-associated viral vectors (rAAV) with a calcium indicator of the latest generation GCaMP6m that are usually applied for the dorsal hippocampus of mice but were not previously used for gene delivery into fish brain. The aim of our work was to study the feasibility of transduction of rAAV in the mouse hippocampus into brain cells of juvenile chum salmon and subsequent determination of the phenotype of rAAV-labeled cells by confocal laser scanning microscopy (CLSM).

Edible Far Eastern Ferns as a Dietary Source of Long-Chain Polyunsaturated Fatty Acids.

Young fronds of ferns are consumed as a vegetable in many countries. The aim of this study was to analyze three fern species that are available for sale in the Russian Far East as dietary sources in terms of fatty acids that are important for human physiology: arachidonic acid (20:4n-6, ARA), eicosapentaenoic acid (20:5n-3, EPA) and other valuable long-chain polyunsaturated fatty acids. The content of ARA and EPA was 5.

Some Examples of the Use of Molecular Markers for Needs of Basic Biology and Modern Society.

Application of molecular genetic markers appeared to be very fruitful in achieving many goals, including (i) proving the theoretic basements of general biology and (ii) assessment of worldwide biodiversity. Both are provided in the present meta-analysis and a review as the main signal. One of the basic current challenges in modern biology in the face of new demands in the 21st century is the validation of its paradigms such as the synthetic theory of evolution (STE) and biological species concept (BSC).

microalga physiological and ultrastructural changes under copper intoxication.

The present work assessed the effect of copper (Cu) on cell dynamics and structure of the microalga (Rhodophyta, Bangiophycidae). Ultrastructure of the microalga was investigated and fluorescence of chlorophyll and phycoerythrin, and content of reactive oxygen species (ROS) were estimated by flow cytometry. The number of cells did not show statistically significant differences at concentrations of 50 and 100 μg/L of Cu compared to the control, whereas 150 μg/L of Cu inhibited population growth.

Action of alkyl glycerol ethers and n-3 polyunsaturated fatty acids diet on hematological parameters of blood and liver plasmalogen level in aged rats.

One of the ways to reduce age-related changes can be a diet correction by adding biologically active substances. We studied the effect of a diet including alkyl glycerol ethers (AGs) and n-3 polyunsaturated fatty acid (PUFA) concentrate isolated from the hepatopancreas of Berrytheuthis magister squid on hematological parameters and plasmalogens level in the liver of elderly rats. The senile animals showed decrease in hemoglobin, a three-fold decrease in leukocytes, a three-fold increase in platelet count, and a double decrease of blood coagulation time in the peripheral blood.

Aquatic toxicity of particulate matter emitted by five electroplating processes in two marine microalgae species.

Electroplating is a widely used group of industrial processes that make a metal coating on a solid substrate. Our previous research studied the concentrations, characteristics, and chemical composition of nano- and microparticles emitted during different electroplating processes. The objective of this study was to evaluate the environmental toxicity of particulate matter obtained from five different electrochemical processes.

Physiological processes and lipidome dynamics in the soft coral Sinularia heterospiculata under experimental bleaching.

Coral polyps host intracellular symbiotic dinoflagellates (SD). The loss of SD (referred as bleaching) under stressful environmental conditions is the main reason of coral reef destruction, and therefore, intensively studied over the world. Lipids are the structural base of biomembranes and energy reserve of corals and are directly involved in the coral bleaching.

The Potency of Seaweed Sulfated Polysaccharides for the Correction of Hemostasis Disorders in COVID-19.

Hemostasis disorders play an important role in the pathogenesis, clinical manifestations, and outcome of COVID-19. First of all, the hemostasis system suffers due to a complicated and severe course of COVID-19. A significant number of COVID-19 patients develop signs of hypercoagulability, thrombocytopenia, and hyperfibrinolysis.

First next-generation sequencing data for Haploporidae (Digenea: Haploporata): characterization of complete mitochondrial genome and ribosomal operon for Parasaccocoelium mugili Zhukov, 1971.

The first data on a whole mitochondrial genome of Haploporidae, Parasaccocoelium mugili (Digenea: Haploporata: Haploporidae) was generated using the next-generation sequencing (NGS) approach. We sequenced the complete mitochondrial DNA (mtDNA) and ribosomal operon of Parasaccocoelium mugili, intestine parasite of mullet fish. The mtDNA of P.

Harmful algal blooms and associated fisheries damage in East Asia: Current status and trends in China, Japan, Korea and Russia.

Occurrences of harmful algal blooms (HABs) and associated fisheries damage have been continuously monitored since the 1970s along the coasts of East Asia. Fisheries damage comprises mass mortalities of fish and shellfish mainly by harmful dinoflagellates and raphidophytes (e.g.