sp. nov.: a novel yeast isolated from subtropical regions in Japan.

Seven yeast strains, NBRC 11072, NBRC 11076, NBRC 11078, NBRC 11080, NBRC 116645, NBRC 116646 and NBRC 116647, were isolated from the subtropical regions of Japan. The seven strains share identical nucleotide sequences in the D1/D2 domains of the large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) regions. Six strains (NBRC 11072, NBRC 11076, NBRC 11078, NBRC 11080, NBRC 116645 and NBRC 116646) share identical mitochondrial cytochrome c oxidase subunit II (COX II) sequences as well, while NBRC 116647 differs from them by one substitution in this region.

Polyphasic Identification of Rhizomonospora bruguierae gen. nov., sp. nov., Isolated from Mangrove Rhizosphere Soil.

A novel yellowish-white actinobacterial strain, designated as TS60-4C, was isolated from the rhizosphere soil of the mangrove Bruguiera gymnorhiza (L.) Lam. in Okinawa, Japan, and was subjected to a polyphasic assessment.

Streptomyces yaizuensis sp. nov., a berninamycin C-producing actinomycete isolated from sponge.

While screening for antibiotics in a marine sample, we discovered a berninamycin C-producing actinomycete, designated YSPA8, isolated from a sponge. A polyphasic approach was used to determine the taxonomic position of the strain. Strain YSPA8 formed sympodially branched aerial mycelia that ultimately segment into chains of spores.

Clear zone formation in microdroplets for high-throughput screening for lactic acid bacteria.

Droplet microfluidic-based technology is a powerful tool for biotechnology, and it is also expected that it will be applied to culturing and screening methods. Using this technology, a new high-throughput screening method for lactic acid bacteria was developed. In this study, the conventional culture of lactic acid bacteria that form clear zones on an agar medium was reproduced in water-in-oil droplets, and only the droplets in which lactic acid bacteria grew were collected one by one.

Recent studies on aero-aquatic fungi, with special reference to diversity of conidial morphology and convergent evolution.

Aero-aquatic fungi compose an ecological group of saprophytes inhabiting the submerged decaying substrates in stagnant freshwater environment. They produce three-dimensional shaped, multi-cellular conidia, which float on water surface by holding air between conidial cells. Because the conidia show diverse morphology, genus and species level classification have been based on their features.

Actinoplanes kirromycinicus sp. nov., isolated from soil.

A novel actinomycete, designated as TPMA0078, was isolated from a soil sample collected in Shinjuku, Tokyo, Japan. 16S rRNA gene sequence analysis indicated that strain TPMA0078 belongs to the genus Actinoplanes and is closely related to Actinoplanes regularis IFO 12514 (99.86% 16S rRNA gene sequence similarity).

Integrated pathway mining and selection of an artificial CYP79-mediated bypass to improve benzylisoquinoline alkaloid biosynthesis.

Background: Computational mining of useful enzymes and biosynthesis pathways is a powerful strategy for metabolic engineering. Through systematic exploration of all conceivable combinations of enzyme reactions, including both known compounds and those inferred from the chemical structures of established reactions, we can uncover previously undiscovered enzymatic processes. The application of the novel alternative pathways enables us to improve microbial bioproduction by bypassing or reinforcing metabolic bottlenecks.

sp. nov., isolated from human faeces.

Two Gram-stain-negative, straight rods, non-motile, asporogenous, catalase-negative and obligately anaerobic butyrate-producing strains, HLW78 and CYL33, were isolated from faecal samples of two healthy Taiwanese adults. Phylogenetic analyses of 16S rRNA and DNA mismatch repair protein MutL () gene sequences revealed that these two novel strains belonged to the genus . On the basis of 16S rRNA and gene sequence similarities, the type strains AF52-21(98.

sp. nov., a novel actinobacterium isolated from flowerpot soil.

Strain CJN36-1N, a Gram-stain-positive, non-flagellated, strictly aerobic and short rod-shaped bacterium, was isolated from flowerpot soil sampled in the Jeonju region of the Republic of Korea. Based on 16S rRNA gene sequences and the resulting phylogenetic tree, the strain belonged to the genus . Strain CJN36-1N contained a chromosome of 3.

sp. nov., Isolated from Soymilk.

A novel coagulase-negative strain (H164) was isolated from soymilk in Taiwan. Comparative sequence analysis of the 16S rRNA gene revealed that the H164 strain is a member of the genus . We used multilocus sequence analysis (MLSA) and phylogenomic analyses to demonstrate that the novel strain was closely related to , , , and .

Reclassification of a reveromycin-producer and the proposals of Actinacidiphila reveromycinica sp. nov. and Actinacidiphila acidipaludis comb. nov.

Streptomyces sp. SN-593, a reveromycin producer, was previously thought to belong to the genus Streptomyces based on its morphological and chemotaxonomic characteristics. In this paper, we re-considered its taxonomic position according to the current criteria.

Streptococcus taonis sp. nov., a novel bacterial species isolated from a blood culture of a patient.

One Gram stain-positive, catalase-negative, α-hemolytic, chain-forming or paired cocci, designated ST22-14, was isolated from a blood culture of a child with suspected infection. The results of 16S rRNA gene sequences analyses showed that the most closely related species to strain ST22-14 were "Streptococcus vulneris" DM3B3 (99.2%), Streptococcus mitis NCTC 12261 (99.

Genomic diversity of the pathogenic fungus Aspergillus fumigatus in Japan reveals the complex genomic basis of azole resistance.

Aspergillus fumigatus is a pathogenic fungus with a global distribution. The emergence of azole-resistant A. fumigatus (ARAf) other than the TR-mutants is a problem in Japan.

Application of MicroResp™ for quick and easy detection of plastic degradation by marine bacterial isolates.

Microplastic debris in the marine environment is a global problem. Biodegradable polymers are being developed as alternatives to petroleum-based plastics, and quick and easy methods for screening for bacterial strains that can degrade such polymers are needed. As a screening method, the clear zone method has been widely used but has technical difficulties such as plate preparation and interpretation of results.

f.a., sp. nov., a novel yeast isolated from flowers.

Three yeast isolates, NBRC 115909, NBRC 115910 and NBRC 116270, were isolated from (red clover) flowers collected from Kisarazu, Chiba, Japan. Analysis of the sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions revealed that these isolates represent a single novel species within the genus . Also, no ascospore formation was observed.

sp. nov., an actinomycete isolated from soil.

A novel actinomycete, designated RD004123, was isolated from a soil sample collected in Hokkaido, Japan, and its taxonomic position was investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain RD004123 fell within the cluster of the family but did not form a reliable cluster with any member of the family. The similarity values between strain RD004123 and the type species of 29 genera in the family were 91.

sp. nov., isolated from Japanese faecal samples.

Two Gram-positive, anaerobic, non-spore-forming and coccoid or oval-shaped bacterial strains, namely, DN0138 and DN0266, were isolated from faecal samples of healthy Japanese people. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DN0138 clustered with a species of the genus and was closely related to JCM 1471, JCM 1395, KB1 and '' Marseille-P2377, with sequence similarities of 98.6, 98.

sp. nov., sp. nov. and sp. nov., isolated from saltern soil.

Three bacterial strains, designated SSBR10-3, SSTM10-2 and SSHM10-5, were isolated from saltern soil sampled in Jeollanam-do, Republic of Korea. Cells were aerobic, Gram-stain-positive, flagellated and rod-shaped. The strains grew optimally at 28°C and at pH 7.

sp. nov. and sp. nov., halotolerant bacteria isolated from a saltern environment.

Two aerobic, Gram-stain-positive, spore-forming motile bacterial strains, designated SSPM10-3 and SSWR10-1, were isolated from salterns in Jeollanam province of South Korea. Both strains were halotolerant and grew well in 5 % NaCl but not in 20 and 25% NaCl, respectively. Optimal growth was observed with 5 % NaCl, at 30 °C and at pH 7.