158 results match your criteria: "National Institute of Technology and Evaluation NITE.[Affiliation]"

Complete genome sequence of Selenomonas ruminantium subsp. lactilytica will accelerate further understanding of the nature of the class Negativicutes.

FEMS Microbiol Lett

May 2015

Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori, Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan Department of Human Health and Nutrition, Graduate School of Comprehensive Human Sciences, Shokei Gakuin University, 4-10-1 Yurigaoka, Natori 981-1295, Japan.

Selenomonas ruminantium subsp. lactilytica, a strictly anaerobic ruminal bacterium, possesses typical Gram-negative cell surface structure comprising cytoplasmic membrane, peptidoglycan layer and outer membrane, whereas its 16S rRNA-based taxonomy shows that the bacteria belongs to Gram-positive Firmicutes. Complete genome analysis showed that genes or gene clusters involved in Gram-negative cell structure were scattered in the S.

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We report the draft genome sequence of Cupriavidus pauculus strain KF709, which comprises 6,826,799 bp with 6,272 coding sequences. The strain KF709 utilizes biphenyl and degrades low-chlorinated biphenyls; however, it possesses fewer coding sequences involved in the degradation of aromatic compounds than other strains belonging to the Betaproteobacteria.

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We report the draft genome sequence of Cupriavidus basilensis KF708 (NBRC 110671), which utilizes biphenyl as a sole carbon source and degrades polychlorinated biphenyls (PCBs). The KF708 strain possesses genes for biphenyl catabolism and other genes involved in various aromatic compounds.

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Pseudomonas putida KF703 (NBRC 110666) utilizes biphenyl as a sole source of carbon and degrades polychlorinated biphenyls (PCBs). Here, we report the draft genome sequence of the KF703 strain, which provides insight into the molecular mechanisms of adaptation to an environment polluted by aromatic compounds.

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Pseudomonas abietaniphila KF717 utilizes biphenyl as a sole source of carbon and energy and degrades polychlorinated biphenyls (PCBs). We report here the 6,930,016-bp genome sequence of this strain, which contains 6,323 predicted coding sequences (CDSs), including the biphenyl-utilizing bph gene cluster.

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Iron corrosion induced by nonhydrogenotrophic nitrate-reducing Prolixibacter sp. strain MIC1-1.

Appl Environ Microbiol

March 2015

National Institute of Technology and Evaluation (NITE) Biological Resource Center (NBRC), NITE, Kisarazu, Chiba, Japan Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, Bunkyo, Tokyo, Japan.

Microbiologically influenced corrosion (MIC) of metallic materials imposes a heavy economic burden. The mechanism of MIC of metallic iron (Fe(0)) under anaerobic conditions is usually explained as the consumption of cathodic hydrogen by hydrogenotrophic microorganisms that accelerates anodic Fe(0) oxidation. In this study, we describe Fe(0) corrosion induced by a nonhydrogenotrophic nitrate-reducing bacterium called MIC1-1, which was isolated from a crude-oil sample collected at an oil well in Akita, Japan.

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A novel, obligately chemolithoautotrophic, sulfur-oxidizing bacterial strain, designated strain gps52(T), was isolated from a rock sample collected near the hydrothermal vents of the Suiyo Seamount in the Pacific Ocean. The cells possessed a Gram-stain-negative-type cell wall and contained menaquinone-8(H4) and menaquinone-9(H4) as respiratory quinones, and C16 : 1ω7c, C16 : 0 and C18 : 1ω7c as major cellular fatty acids. Neither storage compounds nor extensive internal membranes were observed in the cells.

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Elemental iodine is produced in Japan from underground brine (fossil salt water). Carbon steel pipes in an iodine production facility at Chiba, Japan, for brine conveyance were found to corrode more rapidly than those in other facilities. The corroding activity of iodide-containing brine from the facility was examined by immersing carbon steel coupons in "native" and "filter-sterilized" brine samples.

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We isolated two strains of a novel Lipomyces species from soil collected in Chichibu forest, Saitama prefecture, Japan. Based on their morphological and biochemical characteristics, along with multilocus sequence typing using the D1/D2 domain of the large-subunit (LSU) rRNA gene, the internal transcribed spacer (ITS) region and the translation elongation factor 1 alpha gene (EF-1α), the two strains were shown to represent a novel species of the genus Lipomyces, described as Lipomyces chichibuensis sp. nov.

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Two thermophilic, strictly anaerobic, Gram-negative bacteria, designated strains AZM34c06(T) and AZM44c09(T), were isolated from terrestrial hot springs in Japan. The optimum growth conditions for strain AZM34c06(T) were 60 °C, pH 7.4 and 0% additional NaCl, and those for strain AZM44c09(T) were 70 °C, pH 7.

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Hannaella pagnoccae sp. nov., a tremellaceous yeast species isolated from plants and soil.

Int J Syst Evol Microbiol

June 2014

Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, Brazil.

Several independent surveys of yeasts associated with different plant materials and soil led to the proposal of a novel yeast species belonging to the Tremellales clade (Agaricomycotina, Basidiomycota). Analysis of the sequences of the D1/D2 domains and internal transcribed spacer region of the large subunit of the rRNA gene suggested affinity to a phylogenetic lineage that includes Hannaella coprosmaensis, Hannaella oryzae and Hannaella sinensis. Thirty-two isolates were obtained from different sources, including bromeliads, nectar of Heliconia psittacorum (Heliconiaceae), flowers of Pimenta dioica (Myrtaceae), roots and leaves of sugar cane (Saccharum spp.

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Wickerhamomyces siamensis sp. nov., a novel yeast species isolated from the phylloplane in Thailand.

Int J Syst Evol Microbiol

April 2013

Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Strain DMKU-RK359(T), representing a novel yeast species, was isolated from the external surface of a sugar-cane leaf collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 region of the large-subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, strain DMKU-RK359(T) was assigned to a novel Wickerhamomyces species. The novel species was closest to Wickerhamomyces ciferrii, but differed from it by 0.

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Roseimicrobium gellanilyticum gen. nov., sp. nov., a new member of the class Verrucomicrobiae.

Int J Syst Evol Microbiol

June 2013

Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

The taxonomic properties of strain DC2a-G7(T), a Gram-negative, ovoid to rod-shaped, gellan gum-lysing bacterium, were examined. The 16S rRNA gene sequence similarity showed that DC2a-G7(T) is a member of the phylum Verrucomicrobia and the closest type strain of a species with a validly published name is Verrucomicrobium spinosum DSM 4136(T), with a sequence similarity of 91.2%.

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Article Synopsis
  • * Phylogenetic analysis places these strains within the Wickerhamomyces clade, with high confidence in their grouping based on rRNA gene sequences.
  • * Strain ST-392 is classified as Wickerhamomyces tratensis sp. nov. due to its unique ascospores, while ST-382 is identified as Candida namnaoensis sp. nov., and they differ in fermentation capabilities and nutrient assimilation.
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Among methanogens, only 2 genera, Methanosaeta and Methanosarcina, are known to contribute to methanogenesis from acetate, and Methanosaeta is a specialist that uses acetate specifically. However, Methanosaeta strains so far have mainly been isolated from anaerobic digesters, despite the fact that it is widespread, not only in anaerobic methanogenic reactors and freshwater environments, but also in marine environments, based upon extensive 16S rRNA gene-cloning analyses. In this study, we isolated an aceticlastic methanogen, designated strain 03d30q(T), from a tidal flat sediment.

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A Gram-reaction-positive aerobic actinomycete, designated strain MN08-A0118(T), which produced short chains of non-motile spores on the tips of long sporophores and formed yellow-brown colonies with branched substrate mycelium, was studied in detail to determine its taxonomic position. On the basis of 16S rRNA gene sequence analyses, strain MN08-A0118(T) was grouped into the genus Herbidospora, being most closely related to Streptosporangium claviforme (98.2%), Herbidospora osyris (98.

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Cryptosporangium mongoliense sp. nov., isolated from soil.

Int J Syst Evol Microbiol

October 2012

Biotechnology Development Center, Department of Biotechnology, National Institute of Technology and Evaluation (NITE), Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.

A Gram-positive, aerobic, non-motile actinomycete, strain MN08-A0264(T), was isolated from soil sampled in Mongolia. The isolate formed pale to moderate yellowish brown colonies and branched substrate mycelium. On the basis of 16S rRNA gene sequence analysis, strain MN08-A0264(T) belonged to the genus Cryptosporangium and exhibited 97.

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Four strains of yeasts isolated in Thailand and Taiwan were found to represent four distinct novel species of the ascomycetous anamorphic yeast genus Candida. These strains are located in the Clavispora-Metschnikowia clade in a phylogenetic tree based on the D1/D2 domain sequences of the large subunit rRNA genes. Together with Candida picinguabensis and Candida saopaulonensis, the four novel species constitute a well-separated subclade from other species of the Clavispora-Metschnikowia clade.

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In the present study, an attempt was made to compare the statistical tools used for analysing the data of repeated dose toxicity studies with rodents conducted in 45 countries, with that of Japan. The study revealed that there was no congruence among the countries in the use of statistical tools for analysing the data obtained from the above studies. For example, to analyse the data obtained from repeated dose toxicity studies with rodents, Scheffé's multiple range and Dunnett type (joint type Dunnett) tests are commonly used in Japan, but in other countries use of these statistical tools is not so common.

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A novel moderately thermophilic, red-pigmented bacterium, designated strain AZM34c11(T), was isolated from the bottom of a 1000 m-deep drilled well located in a Japanese hot spring. Cells were Gram-negative and grew optimally at 50 °C, at pH 7.6 and with 0-0.

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In order to understand the influence of coefficient of variation (CV) in determining significant difference of quantitative values of 28-day repeated-dose toxicity studies, we examined 59 parameters of 153 studies conducted in accordance with Chemical Substance Control Law in 12 test facilities. Sex difference was observed in 12 parameters and 10 parameters showed large CV in females. The minimum CV was 0.

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A new Barnettozyma species forming hat-shaped ascospores isolated from soil in Japan.

J Gen Appl Microbiol

August 2011

NITE Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), Kisarazu, Chiba, Japan.

In the course of a study on yeast diversity in Japan, we isolated 331 yeast strains from natural substrates in Rishiri Island, which belongs to the subarctic zone. Among the isolates from soil, two strains produced hat-shaped ascorspores and showed that reproduction occurred by conjugation of a larger cell and a smaller one. We surveyed strains preserved in our culture collection, NBRC, and found one Barnettozyma strain; thus we examined these three strains.

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