[ALLERGIC CONTACT DERMATITIS DUE TO NITRILE RUBBER GLOVES: ETHYL ISOTHIOCYANATE AND BUTYL ISOTHIOCYANATE AS POSSIBLE CAUSATIVE CHEMICALS].

Background: The causative chemicals responsible for nitrile rubber glove-induced allergic contact dermatitis have not been fully elucidated.

Subject: This case involved a 36-year-old female, who developed an erythematous rash on her hands after one and a half months of wearing nitrile rubber gloves at her workplace.

Methods: Patch tests were performed using the gloves as is, and the Japanese standard allergen 2008 and their components.

Phylogenetic characterization of two novel species of the genus Bifidobacterium: Bifidobacterium saimiriisciurei sp. nov. and Bifidobacterium platyrrhinorum sp. nov.

Three bifidobacterial Gram-stain-positive, non-spore forming and fructose-6-phosphate phosphoketolase-positive strains, SMA1, SMB2 and SMA15 were isolated from the faeces of two adult males of the squirrel monkey (Saimiri sciureus). On the basis of 16S rRNA gene sequence similarities, the type strain of Bifidobacterium primatium DSM 100687 (99.3%; similarity) was the closest neighbour to strains SMA1 and SMB2, whereas the type strain of Bifidobacterium stellenboschense DSM 23968 (96.

sp. nov., isolated from a fish farm.

An obligate aerobic and bacteriochlorophyll -containing bacterium, designated strain AI77, was isolated from a fish farm in Uwa Sea, Japan. Cells were Gram-stain-negative, coccoid- to oval-shaped, and showed no motility. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain AI77 is a member of the genus and closely related to MM-7 (97.

EURL ECVAM Genotoxicity and Carcinogenicity Database of Substances Eliciting Negative Results in the Ames Test: Construction of the Database.

The bacterial reverse mutation test (Ames test) is the most commonly used genotoxicity test; it is a primary component of the chemical safety assessment data required by regulatory agencies worldwide. Within the current accepted in vitro genotoxicity test battery, it is considered capable of revealing DNA reactivity, and identifying substances that can produce gene mutations via different mechanisms. The previously published consolidated EURL ECVAM Genotoxicity and Carcinogenicity Database, which includes substances that elicited a positive response in the Ames test, constitutes a collection of data that serves as a reference for a number of regulatory activities in the area of genotoxicity testing.

Alternative strategies of nutrient acquisition and energy conservation map to the biogeography of marine ammonia-oxidizing archaea.

Ammonia-oxidizing archaea (AOA) are among the most abundant and ubiquitous microorganisms in the ocean, exerting primary control on nitrification and nitrogen oxides emission. Although united by a common physiology of chemoautotrophic growth on ammonia, a corresponding high genomic and habitat variability suggests tremendous adaptive capacity. Here, we compared 44 diverse AOA genomes, 37 from species cultivated from samples collected across diverse geographic locations and seven assembled from metagenomic sequences from the mesopelagic to hadopelagic zones of the deep ocean.

Holocarpic oomycete parasitoids of red algae are not .

is a genus of obligate holocarpic endobiotic oomycetes. Most of the species classified in the genus are known only from their morphology and life cycle, and a few have been examined for their ultrastructure or molecular phylogeny. However, the taxonomic placement of all sequenced species is provisional, as no sequence data are available for the type species, , to consolidate the taxonomy of species currently placed in the genus.

Aerobic degradation of cis-dichloroethene by the marine bacterium Marinobacter salsuginis strain 5N-3.

An aerobic bacterium, designated strain 5N-3 (NBRC 113055), that degrades cis-dichloroethene (cDCE) was isolated from a sea sediment in Japan. Strain 5N-3 was able to degrade a certain amount of cDCE in the presence of pyruvate without the action of inducers. In the presence of inducers, such as phenol and benzene, the strain completely removed cDCE.

Complete Genome Sequence of Blautia producta JCM 1471.

We report a complete genome sequence of JCM 1471 The genome consists of a single circular chromosome of 6,197,116 bp with a G+C content of 45.7%. The genome was annotated as containing 5 complete sets of rRNA genes, 70 tRNA genes, and 5,516 protein-coding sequences.

Complete Genome Sequence of Flavonifractor plautii JCM 32125.

We report the complete genome sequence of JCM 32125 (=VPI 0310). The genome consists of a single circular chromosome of 3,985,392 bp (G+C content, 60.9%) and was predicted to contain 3 complete sets of rRNA genes, 63 tRNA genes, and 3,764 protein-coding sequences.

Complete Genome Sequence of Megamonas funiformis JCM 14723.

We announce the complete genome sequence of JCM 14723 (YIT 11815). The genome consists of a circular chromosome (2,522,577 bp, 31.5% G+C content) and a plasmid of 46,189 bp (29.

Complete Genome Sequence of Collinsella aerofaciens JCM 10188.

We report a complete genome sequence of JCM 10188 (=VPI 1003). The genome consists of a circular chromosome (2,428,218 bp with 60.6% G+C content) and two extrachromosomal elements.

sp. nov., a novel lactic acid bacterium isolated from lychee.

A novel lactic acid bacterium, strain MB7, was isolated from lychee in Taiwan. MB7 is Gram-staining-positive, catalase-negative, non-motile, non-haemolytic, facultatively anaerobic, coccoid-shaped, heterofermentative and mainly produces d-lactic acid from glucose. Comparative analysis of 16S rRNA, and gene sequences has demonstrated that the novel strain represented a member of the genus .

sp. nov., isolated from faeces of Formosan barking deer ().

A Gram-stain-positive strain, 8 H-2, was isolated from faeces of Reeves' muntjac () barking deer in Taiwan. Cells of the strain were short rod-shaped, non-motile, non-haemolytic, asporogenous, facultatively anaerobic, heterofermentative and did not exhibit catalase and oxidase activities. Comparative analyses of 16S rRNA, and gene sequences demonstrated that the novel strain was a member of the genus .

Fourteen novel lipomycetaceous yeast species isolated from soil in Japan and transfer of to the genus based on ascospore production phenotype.

Fourteen novel lipomycetaceous yeasts species were isolated from soil samples collected from the Hokkaido, Chiba and Okinawa prefectures of Japan. Phylogenetic analyses of the D1/D2 domains of the large subunit rRNAs and translation elongation factor 1 alpha genes () revealed that five strains of two species from the soil in Furano-shi, Hokkaido were related to and 29 strains representing 12 species from soils in Kamogawa-shi, Chiba and Iriomote Island, Okinawa were in the clade. The two species of form globose or ellipsoid ascospores in their sac-like ascus and pseudohyphae.

sp. nov., isolated from brewer's grain used to make silage in Taiwan.

A Gram-stain-positive, coccus- or oval-shaped, non-motile, haemolytic, asporogenous, catalase- and oxidase-negative, and facultatively anaerobic strain, 2B-2, was isolated from a brewer's grain used to make silage in Taiwan. Comparative analyses of 16S rRNA, and gene sequences demonstrated that strain 2B-2 was a member of the genus . On the basis of 16S rRNA gene sequence similarity, the type strains of (98.

f.a., sp. nov., a novel yeast species producing two kinds of lipases with activity at different temperatures.

Two yeast strains isolated from soil collected in Hokkaido, Japan, were found to secrete two extracellular lipases that exhibited activities at both 25 and 4 °C. Both strains could utilize olive oil, rapeseed oil, lard and fish oil as sole carbon sources. The similarity of the D1/D2 domain of the large subunit ribosomal RNA (LSU rRNA) sequence of these yeast strains to that of other yeasts in the GenBank database was very low (<96 %).

sp. nov., the first aerobic bacteriochlorophyll -containing fresh water bacterium in the genus of the class .

A strictly aerobic, bacteriochlorophyll -containing betaproteobacterium designated strain W35, was isolated from a biofilm sampled at Tama River in Japan. The non-motile and rod-shaped cells formed pink-beige pigmented colonies on agar plates containing organic compounds, and showed an absorption maximum at 871 nm in the near-infrared region, typical for the presence of bacteriochlorophyll . The new bacterial strain is Gram-negative, and oxidase- and catalase-positive.

Characterization of Bifidobacterium species in feaces of the Egyptian fruit bat: Description of B. vespertilionis sp. nov. and B. rousetti sp. nov.

Fifteen bifidobacterial strains were obtained from faeces of Rousettus aegyptiacus; after grouping them by RAPD PCR only eight were selected and characterized. Analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dna G) genes revealed that these eight strains were classified into five clusters: Cluster I (RST 8 and RST 16), Cluster II (RST 9 and RST 27), Cluster III (RST 7 and RST 11), Cluster IV (RST 19), Cluster V (RST 17) were closest to Bifidobacterium avesanii DSM 100685 (96.3%), Bifidobacterium callitrichos DSM 23973 (99.

Enterococcus florum sp. nov., isolated from a cotton flower (Gossypium hirsutum L.).

A Gram-stain-positive and catalase negative coccus, designated strain Gos25-1, isolated from a cotton flower (Gossypium hirsutum L.) collected from Khao Wong district, Kalasin province, Thailand. The taxonomic position of this strain was systematically studied based upon polyphasic taxonomic methods.

sp. nov. and sp. nov., isolated from suan-tsai, a traditional fermented mustard green product of Taiwan.

Four Gram-stain-positive strains, R7, R11, R19 and R27, were isolated from suan-tsai, a traditional fermented mustard green product of Taiwan. Cells were rod-shaped, non-motile, non-haemolytic, asporogenous, facultatively anaerobic, heterofermentative, and did not exhibit catalase and oxidase activities. Comparative analyses of 16S rRNA, and gene sequences demonstrated that these novel strains were members of the genus .

Lactobacillus suantsaii sp. nov., isolated from suan-tsai, a traditional Taiwanese fermented mustard green.

A Gram-stain-positive, rod-shaped, non-motile, catalase-negative and facultative anaerobic strain, L88, was isolated from suan-tsai, a traditional Taiwanese fermented mustard green. Comparative analyses of 16S rRNA, pheS and rpoA gene sequences demonstrated that strain L88was a member of the genus Lactobacillus. On the basis of 16S rRNA gene sequence similarity, the type strains of Lactobacillus acidifarinae (98.

Ecological impact assessment of a bioaugmentation site on remediation of chlorinated ethylenes by multi-omics analysis.

Bioremediation may affect the ecological system around bioremediation sites. However, little is known about how microbial community structures change over time after the initial injection of degraders. In this study, we have assessed the ecological impact of bioaugmentation using metagenomic and metatranscriptomic approaches to remove trichlorinated ethylene/cis-dichloroethylene (TCE/cDCE) by Rhodococcus jostii strain RHA1 as an aerobic chemical compound degrader.

An extracellular [NiFe] hydrogenase mediating iron corrosion is encoded in a genetically unstable genomic island in Methanococcus maripaludis.

Certain methanogens deteriorate steel surfaces through a process called microbiologically influenced corrosion (MIC). However, the mechanisms of MIC, whereby methanogens oxidize zerovalent iron (Fe), are largely unknown. In this study, Fe-corroding Methanococcus maripaludis strain OS7 and its derivative (strain OS7mut1) defective in Fe-corroding activity were isolated.

The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina).

Pseudozyma antarctica and Malassezia furfur are basidiomycetous yeasts under the subphylum Ustilaginomycotina. P. antarctica is a commensal organism found in certain plant species, while M.