Pre-validation of a novel reconstructed skin equivalent model for skin irritation and nanoparticle risk assessment.

In alignment with the global movement toward reducing animal testing, several reconstructed human epidermis (RHE) models have been created for conducting skin irritation tests. These models have undergone development, verification, validation, and integration into OECD TG 439. Our team has introduced a novel in-house RHE named GB-RHE, and we adhere to OECD TG 439 to pre-validate the model and test its potential employment for nanoparticle irritation studies.

iDIA-QC: AI-empowered data-independent acquisition mass spectrometry-based quality control.

Quality control (QC) in mass spectrometry (MS)-based proteomics is mainly based on data-dependent acquisition (DDA) analysis of standard samples. Here, we collect 2754 files acquired by data independent acquisition (DIA) and paired 2638 DDA files from mouse liver digests using 21 mass spectrometers across nine laboratories over 31 months. Our data demonstrate that DIA-based LC-MS/MS-related consensus QC metrics exhibit higher sensitivity compared to DDA-based QC metrics in detecting changes in LC-MS status.

Acoustic frequency comb generation on a composite diamond/silicon microcantilever in ambient air.

Acoustic frequency combs (AFCs) contain equidistant coherent signals with unconventional possibilities on metrology. Previously, implementation of AFCs on mechanical microresonators with large air damping loss is difficult, which restricted their atmospheric applications. In this work, we explore the potentials of a composite diamond/silicon microcantilever for parametric generation of AFCs in ambient air.

Premelted-Substrate-Promoted Selective Etching Strategy Realizing CVD Growth of High-Quality Graphene on Dielectric Substrates.

Direct chemical vapor deposition growth of high-quality graphene on dielectric substrates is a great challenge. Graphene growth on dielectrics always suffers from the issues of a high nucleation density and poor quality. Herein, a premelted-substrate-promoted selective etching (PSE) strategy was proposed.

Standard: Human gastric organoids.

Organoid technology provides a transformative approach to understand human physiology and pathology, offering valuable insights for scientific research and therapeutic development. Human gastric organoids, in particular, have gained significant interest for applications in disease modeling, drug discovery, and studies of tissue regeneration and homeostasis. However, the lack of standardized quality control has limited their extensive clinical applications.

Silver Nanoparticles-Functionalized Textile against SARS-CoV-2: Antiviral Activity of the Capping Oleylamine Molecule.

COVID-19 disease, triggered by SARS-CoV-2 virus infection, has led to more than 7.0 million deaths worldwide, with a significant fraction of recovered infected people reporting postviral symptoms. Smart surfaces functionalized with nanoparticles are a powerful tool to inactivate the virus and prevent the further spreading of the disease.

Impact of Long-Term Cold Storage on the Physicochemical Properties, Volatile Composition, and Sensory Attributes of Dried Jujube ( Mill.).

Grey jujube ( Mill. cv. Huizao), a prominent cultivar from Xinjiang, China, is well known for its high nutritional value and medicinal benefits.

Long-Term Success of Dental Implants in Atrophic Maxillae: A 3-Year Case Series Using Hydroxyapatite and L-PRF.

Dental implants are essential for the prosthetic rehabilitation of edentulous patients, requiring adequate bone volume and density for osseointegration and load support. The posterior region of the maxilla, commonly deficient in bone quality and quantity, represents a clinical challenge. This case series reports an analysis involving 69 dental implants in the atrophic maxilla of nine patients.

Nanoscale reference and test materials for the validation of characterization methods for engineered nanomaterials - current state, limitations, and needs.

The rational design of engineered nanomaterials (NMs) with improved functionality and their increasing industrial application requires reliable, validated, and ultimately standardized characterization methods for their application-relevant, physicochemical key properties such as size, size distribution, shape, or surface chemistry. This calls for nanoscale (certified) reference materials (CRMs; RMs) and well-characterized reference test materials (RTMs) termed also quality control (QC) samples, assessed, e.g.

Behind the scenes of EQA-characteristics, capabilities, benefits and assets of external quality assessment (EQA).

The main stakeholders in external quality assessment (EQA) programs are the participants, in whose interests these challenges are ultimately organised. EQA schemes in the medical field contribute to improving the quality of patient care by evaluating the analytical and diagnostic quality of laboratory and point-of-care tests (POCT) by independent third parties and, if necessary, pointing out erroneous measurement results and analytical or diagnostic improvement potential. Other benefits include the option of using EQA samples for other important laboratory procedures, such as the verification or validation of diagnostic medical devices (IVD-MDs), a contribution to the estimation of measurement uncertainty, a means of training and educating laboratory staff through educational EQA programmes or samples, or even for independent and documented monitoring of staff competence, such as on samples with unusual or even exceptional characteristics.

Behind the scenes of EQA - characteristics, capabilities, benefits and assets of external quality assessment (EQA).

External quality assessment (EQA) enhances patient safety through the evaluation of the quality of laboratory-based and point of care testing. Regulatory agencies and accreditation organizations utilize the results and the laboratory's response to them as part of assessing the laboratory's fitness to practice. In addition, where EQA samples are commutable and the assigned value has been determined using reference measurement procedures (RMPs), EQA data contributes to the verification of metrological traceability of assays as part of the post-market surveillance of diagnostic (IVD) medical devices (IVD-MDs).

Hydroxylated magnetic microporous organic network for efficient magnetic solid phase extraction of trace triazine herbicides.

Here we covalently constructed abundant long-chain hydroxyl groups-functionalized magnetic microporous organic networks (MMON-2OH) for detection of eight Triazine herbicides (THs) in honey and water samples. MMON-2OH owned a high surface area (287.86 m²/g), enhanced water compatibility, and increased exposure of long-chain hydroxyl groups, which significantly improved enrichment capacity for THs.

Public exposure from inhalation of radon and thoron around the tin mine and smelter areas in Bangka, Indonesia.

Radon (Rn) and thoron (Rn) were reported as the highest contributors to natural radiation received by humans. Furthermore, radon has been stated as the second-highest cause of lung cancer. The concentrations of U and Th (the parent nuclide of radon and thoron, respectively) in nature vary with geological conditions and can be enhanced by human activities.

Standard: Human gastric cancer organoids.

Gastric cancer is one of the most common malignancies with poor prognosis. The use of organoids to simulate gastric cancer has rapidly developed over the past several years. Patient-derived gastric cancer organoids serve as in vitro models that closely mimics donor characteristics, offering new opportunities for both basic and applied research.

Role of in Filamentous Growth and Pathogenicity of .

is a dimorphic fungus that specifically infects , causing stem swelling and the formation of an edible fleshy stem known as jiaobai. The pathogenicity of is closely associated with the development of jiaobai and phenotypic differentiation. Msb2 acts as a key upstream sensor in the MAPK (mitogen-activated protein kinase) signaling pathway, playing critical roles in fungal hyphal growth, osmotic regulation, maintenance of cell wall integrity, temperature adaptation, and pathogenicity.

The Application of Digital PCR as a Reference Measurement Procedure to Support the Accuracy of Quality Assurance for Infectious Disease Molecular Diagnostic Testing.

Background: Nucleic acid amplification tests (NAATs) assist in the diagnosis of numerous infectious diseases. They are typically sensitive and specific and can be quickly developed and adapted. Far more challenging is the development of standards to ensure NAATs are performing within specification; reference materials take time to develop and suitable reference measurement procedures (RMPs) have not been available.

Development of three biofuel CRMs for the quality parameters in biodiesel and wood pellet via a joint research project.

Biomass is a key element in biofuels which can be defined as a fuel produced through contemporary biological processes, and its increased use can support the EU's aims of reducing greenhouse gas emissions. Information on the nature and the quality of the biomass or biofuel is important in order to support the optimization of their combustion with respect to realizing higher efficiencies and lower emissions during energy production. Three reference materials were produced by a collaborative approach among national metrology institutes and designated institutes within the scope of the EMPIR project: BIOFMET.

An accurate IDMS-based method for absolute quantification of phytohemagglutinin, a major antinutritional component in common bean.

Phytohemagglutinin (PHA), a natural tetramer comprising PHA-E and PHA-L subunits that preferentially bind to red and white blood cells, respectively, constitutes a significant antinutritional and allergenic factor in common bean seeds. The accurate measurement of PHA content is a prerequisite for ensuring food safety inspections and facilitating genetic improvements in common bean cultivars with reduced PHA levels. Currently, mainstream methods for PHA quantification involve hemagglutination assays and immunodetection, but these methods often require fresh animal blood and lack specificity and accuracy.

Spatiotemporal distribution prediction for PM based on STXGBoost model and high-density monitoring sensors in Zhengzhou High Tech Zone, China.

The increasing demand for air pollution control has driven the application of low-cost sensors (LCS) in air quality monitoring, enabling higher observation density and improved air quality predictions. However, the inherent limitations in data quality from LCS necessitate the development of effective methodologies to optimize their application. This study established a hybrid framework to enhance the accuracy of spatiotemporal predictions of PM, standard instrument measurements were employed as reference data for the remote calibration of LCS.

Impact of Cooking on Tuber Color, Texture, and Metabolites in Different Potato Varieties.

Potatoes are a globally important crop with high nutritional value. Different potato varieties display notable variations in color, texture, and nutrient composition. However, the influence of cooking on tuber color, texture, and metabolites has not been comprehensively explored.

Research on the Selective Extraction of Oxibendazole Residues in Animal-Derived Food Using Molecularly Imprinted Polymers.

To achieve specific adsorption-based sample preparation for the poorly soluble veterinary drug oxibendazole, this study employed 4-vinylpyridine as the functional monomer and conducted radical polymerization on the surface of functionalized silica nanoparticles to synthesize a surface molecularly imprinted polymer (OBZMIP). This OBZMIP exhibited good adsorption capacity for oxibendazole within 30 min, with its adsorption behavior conforming to the pseudo-second-order kinetic and Langmuir models, predicting a maximum adsorption capacity of 4.93 mg/g.

Comprehensive host cell proteins profiling in biopharmaceuticals by a sensitivity enhanced mass spectrometry strategy using TMT-labeling and signal boosting.

Background: Host Cell Proteins (HCPs) are impurities expressed in host cells during the biopharmaceutical production process, whichmay compromise product quality and potentially leading to immunogenic reactions or other adverse effects. Mass spectrometry (MS)-based strategy is more and more considered as a promising method for HCPs analysis, since it is capable of simultaneously quantifying thousands of proteins in a single test. However, considering the large excess biopharmaceutical product protein present in the system and the extremely low abundance of HCPs, sensitive MS methods are urgently needed in HCPs analysis.

Implementation of the ISO 33405 requirements and production of a certified reference material for nitrofuran metabolites as incurred chicken muscle.

Certified reference materials (CRMs) contribute to the traceability of measurement results to recognized anchor points, ideally to the International System of Units (SI), and consequently, to their comparability and reliability. In the area of veterinary drugs' residues in foods of animal origin, the important role of CRMs must be highlighted, given the problems caused by trade restrictions in some countries. The results of studies concerning the certification of an incurred matrix certified reference material (IMCRM) for the mass fraction of nitrofuran metabolites in chicken muscles are presented in this work.