75 results match your criteria: "National Institute of Lasers and Optronics College[Affiliation]"

Environmentally hazardous radioactive isotopes of iodine may be released from a nuclear power plant as a by-product of uranium fission. The efficient and safe capture of volatile radioiodine is of great significance in the history of nuclear power plants. Due to its high volatility and carcinogenic characteristics, elimination of iodine gas (I) from air is the need of the hour from an environmental and health point of view.

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Article Synopsis
  • The study evaluates the antibacterial effectiveness of Tryptophan-coordinated silver nanoparticles (Ag-TrpNPs) using conventional and synchronous fluorescence spectroscopy techniques.
  • Ag-TrpNPs are considered promising due to their antibacterial properties, but their potential toxicity necessitates coordination with biocompatible molecules like Tryptophan.
  • Results showed significant zones of inhibition for E. coli, confirming the Ag-TrpNPs' efficacy against bacteria at various concentrations, with optical characterization techniques confirming their size and morphology.
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Diabetes mellitus is a prevalent chronic disease necessitating timely identification for effective management. This paper introduces a reliable, straightforward, and efficient method for the minimally invasive identification of diabetes mellitus through nanosecond pulsed laser-induced breakdown spectroscopy (LIBS) by integrating a state-of-the-art machine learning approach. LIBS spectra were collected from urine samples of diabetic and healthy individuals.

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Rapid detection of bactericidal efficacy of nanoparticles coated polyurethane foam by synchronous fluorescence spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc

December 2024

National Institute of Lasers and Optronics College, Pakistan Institute of Engineering and Applied Sciences, Nilore 45650, Islamabad, Pakistan.

The ability of right-angled synchronous fluorescence spectroscopy (SFS) was explored to analyse the bacterial load in water treated with green synthesized silver nanoparticles (AgNPs) coated polyurethane foam (PUF). Gram negative (Escherichia coli, Pseudomonas aeruginosa) and Gram positive (Staphylococcus aureus) bacteria cultured in nutrient broth were diluted in autoclaved water containing NPs-coated PUF. The survival rate of S.

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Due to antimicrobial drug resistance, there is a growing interest in the development of light based alternative antibacterial therapies. This research work is focused on the inactivation of Escherichia coli (E. coli) by exploiting the absorption bands 405, 505, 542, 580 and 631 nm of its indigenously produced Protoporphyrin IX (PpIX) excited by three LEDs with broad emission bands at 418, 522 and 630 nm and two laser diodes with narrow emission bands at 405 and 635 nm.

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The current study looks into the characterization and differentiation of mango juices that are sold commercially using fluorescence spectroscopy. The emission spectra displayed well-defined and prominent peaks that suggested the existence of many fluorophores, such as water content, β-carotene, tartrazine food color, and chlorophyll components. For this study, water and yellow food coloring solution, the two most popular adulterants were added to pure and authenticated mango pulp that had been diluted to an 8% concentration.

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Utilizing machine learning algorithms for precise discrimination of glycosuria in fluorescence spectroscopic data.

Spectrochim Acta A Mol Biomol Spectrosc

October 2024

Department of Physics, Islamia College Peshawar, Khyber Pakhtunkhwa 25120, Pakistan.

Fluorescence spectroscopy coupled with a random forest machine learning algorithm offers a promising non-invasive approach for diagnosing glycosuria, a condition characterized by excess sugar in the urine of diabetic patients. This study investigated the ability of this method to differentiate between diabetic and healthy control urine samples. Fluorescent spectra were captured from urine samples using a Xenon arc lamp emitting light within the 200 to 950 nm wavelength range, with consistent fluorescence emission observed at 450 nm under an excitation wavelength of 370 nm.

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In this paper, smart integration of cold dielectric barrier discharge (DBD) plasma in various geometrical arrangements with laser ablation at atmospheric pressure for nanomaterial was described. A composite Co:ZnO target was ablated in an airflow by a nanosecond (ns) laser (wavelength: 1064 nm, pulse duration: 30 ns) using fluence of 5 J-cm at a repetition rate of 10 Hz. The nanomaterial produced under vertical and oblique plasma streams, surface discharge and gas flow, were compared.

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We report an efficient sample preparation method (freezing) for onsite fat and meat analysis via a specially designed thermoelectric cooling and temperature-controlling system. This investigation also focused on the effect of phase change on the sensitivity and reproducibility of LIBS emission signals and plasma parameters. The plasma emissions of animal fats (lard) were recorded when the sample was frozen (-2 °C), fluid (15 °C), and in a liquid state (37 °C) with a thermoelectric cooling system.

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Fuel fraud has proliferated due to underlying economic advantage in nearly every nation. For the purpose of detecting adulteration and providing real-time quality assurance, non-destructive oil analysis is crucial. This paper reports the simple approach for fingerprinting undiluted petroleum products including gasoline from various brands, diesel, and kerosene oil in comparison with organic solvents using synchronous fluorescence spectroscopy and hierarchical cluster analysis.

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Background: Early blight (EB) of Tomatoes, caused by Alternaria solani, is a serious fungal disease that adversely affects tomato production. Infection is characterized by dark lesions on leaves, stems, and fruits. Several agrochemicals can be used to control infection, these chemicals may disrupt environmental equilibrium.

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Fluorescence Spectroscopy Based Characterization of Flaxseed Oil.

J Fluoresc

April 2024

Department of Physics, Mirpur University of Science and Technology (MUST), Azad Jammu & Kashmir, 10250, Mirpur, Pakistan.

Fluorescence spectroscopy has been employed for the compositional analysis of flaxseed oil, detection of its adulteration and investigation of the thermal effects on its molecular composition. Excitation wavelengths from 320 to 420 nm have been used to explore the valued ingredients in flaxseed oil. The emission bands of flaxseed oil centred at 390, 414, 441, 475, 515 and 673/720 nm represent vitamin K, isomers of vitamin E, carotenoids and chlorophylls, which can be used as a marker for quality analysis.

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In this article, Fluorescence spectroscopy has been employed for the identification of Pseudomonas aeruginosa (PA) and Escherichia coli (E. coli) in water suspension. Emission spectra of PA and E.

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To investigate the efficacy of Photobiomodulation therapy (PBMT) for the treatment of solitary rectal ulcer syndrome (SRUS). SRUS is a benign disease, diagnosed by symptoms, clinical, and histological findings. PBMT has been reported for the treatment of various inflammation-based diseases including aphthous ulcer, but still no such study on the treatment of SRUS is published.

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In vitro study: methylene blue-based antibacterial photodynamic inactivation of Pseudomonas aeruginosa.

Appl Microbiol Biotechnol

January 2024

Applied Environmental and Geo-Microbiology Lab, Department of Microbiology, Quaid-i-Azam University, Islamabad, 45320, Pakistan.

Pseudomonas aeruginosa is one of the most antibiotic-resistant and opportunistic pathogens in immunocompromised and debilitated patients. It is considered the cause of most severe skin infections and is frequently found in hospital burn units. Due to its high antibiotic resistance, eliminating P.

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Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential.

J Biomol Struct Dyn

September 2024

Biotechnology of Macromolecules Research Group, Department of Life and Earth Sciences, Instituto de Productos Naturales y Agrobiología-Consejo Superior de Investigaciones Científicas, (IPNA-CSIC), Tenerife, Spain.

This report examines the bio-fabrication of silver nanoparticles (Ag-NPs) utilizing AgNO and leaf extract of as the precursor material. In order to maximize the antibacterial efficacy against , , and , the reaction conditions for the green fabrication of Ag-NPs were optimized. A one factor at a time approach (volume concentration of extract, volume concentration of AgNO, pH and temperature) was used to optimize the best condition, and results were assessed through UV-visible spectroscopy and particle size distribution.

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Removal of methyl iodide (CHI) from the air present within nuclear facilities is a critical issue. In case of any nuclear accident, there is a great need to mitigate the radioactive organic iodide immediately as it accumulates in human bodies, causing severe consequences. Current research focuses on removing organic iodides, for which the surface of activated carbon (AC) was modified by impregnating it with different metals individually, i.

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Fluorescence Spectroscopy Based Characterization of Pseudomonas Aeruginosa Suspension.

J Fluoresc

September 2024

National Institute of Lasers and Optronics College, Pakistan Institute of Engineering and Applied Sciences, 45650, Nilore, Islamabad, Pakistan.

In this article, optical characterization of Pseudomonas aeruginosa (PA) suspension has been performed by using Fluorescence spectroscopy. Optical density (OD) and plate count methods have been employed as a reference for the analysis of emission spectra of Pseudomonas aeruginosa in water suspension. Emission spectra of PA suspension has been acquired by using excitation wavelengths from 270 to 420 nm with step of 10 nm to explore its spectral behavior.

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Air pollution in megacities is increasing due to the dense population index, increasing vehicles, industries, and burning activities that negatively impact human health and climate. There is limited study of air pollution in many megacities of the world including Pakistan. Lahore is a megacity in Pakistan in which the continuous investigation of particulate matter is very important.

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The degradation of organic dye pollutants is a critical environmental issue that has garnered significant attention in recent years. To address this problem, we investigated the potential of CaCrO chromite (CCO) as a photocatalyst for the degradation of cationic and anionic dye solutions under sunlight irradiation. CaCrO was synthesized via a sol-gel auto-combustion route and sintered at 900 °C.

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The current study presents a steadfast, simple, and efficient approach for the non-invasive determination of glycosuria of diabetes mellitus using fluorescence spectroscopy. A Xenon arc lamp emitting light in the range of 200-950 nm was used as an excitation source for recording the fluorescent spectra from the urine samples. A consistent fluorescence emission peak of glucose at 450 nm was found in all samples for an excitation wavelength of 370 nm.

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The current study demonstrates the practical application of optical seed priming technology to improve cotton seed germination, plant growth, crop yield, and fiber quality. The hypothesis of this study is that seed irradiation with different colors of light can improve germination and cotton productivity in different environments. In the priming of cotton seeds, a wider range of the light spectrum was used, ranging from ultraviolet (UV) to red wavelengths.

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Validation of conventional and synchronous fluorescence emission of potable water.

Spectrochim Acta A Mol Biomol Spectrosc

September 2023

Agriculture and Biophotonics Division, National Institute of Lasers and Optronics College, Pakistan Institute of Engineering and Applied Sciences, Nilore 45650, Islamabad, Pakistan.

Water from filter plants and bottled water is generally safe to drink but regular quality monitoring of these facilities requires development of quick analytical technique to ensure public safety and health. This study presented the variation of two components in spectra of conventional fluorescence spectroscopy (CFS) and four components in synchronous fluorescence spectroscopy (SFS) to assess the quality of 25 water samples from different sources. Poor quality water either due to organic or inorganic contaminants presented high intensity fluorescence emission in the blue green region and low intensity water Raman peak unlike an intense water Raman peak originated from pure water when excited at 365 nm.

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Article Synopsis
  • - Fluorescence spectroscopy is effectively used to assess microbial load in water, showing a strong correlation with traditional methods like colony forming unit (CFU) and optical density (OD) techniques.
  • - The study demonstrates that a UV dose of 58.9 mJ/cm is needed for 99.99% disinfection at a flow rate of 0.8 l/min, with fluorescence at 360 nm indicating bacterial degradation as UVC exposure increases.
  • - This method is faster and more sensitive than CFU and OD methods, providing real-time monitoring of pathogen levels in drinking water without the need for a 24-hour incubation period.
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