6 results match your criteria: "National Institute of Genetics and Graduate University for Advanced Studies (SOKENDAI)[Affiliation]"
Kinetochore assembly and function through the cell cycle.
Chromosoma
September 2016
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, 565-0871, Japan.
Article Synopsis
- The kinetochore is crucial for chromosome segregation in eukaryotes, acting as a link between spindle microtubules and chromosomes.
- Recent research has shed light on how kinetochores are formed and function, as well as their role in centromere identity.
- The review focuses on the interactions and regulatory mechanisms of key kinetochore components throughout the cell cycle.
Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes.
Methods Mol Biol
December 2017
Department of Molecular Genetics, National Institute of Genetics and Graduate University for Advanced Studies (SOKENDAI), 1111 Yata, Mishima, Shizuoka, 411-8540, Japan.
Article Synopsis
- The kinetochore is crucial for attaching chromosomes to microtubules during mitosis, ensuring accurate chromosome segregation.
- Key components of the kinetochore include histone-fold proteins CENP-S, -T, -W, and -X, which help establish kinetochore chromatin and form both heteromeric and heterotetrameric complexes.
- This study focuses on the methods used to purify and characterize these kinetochore histone-fold complexes to better understand their formation and function.
Dynamic changes in CCAN organization through CENP-C during cell-cycle progression.
Mol Biol Cell
November 2015
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan Department of Molecular Genetics, National Institute of Genetics and Graduate University for Advanced Studies (SOKENDAI), Mishima, Shizuoka 411-8540, Japan
Article Synopsis
- The kinetochore is vital for accurate chromosome separation during cell division, built on a protein network called CCAN at the chromosome's centromere.
- While the CCAN consists of various subcomplexes, how these proteins organize to form a functional kinetochore throughout the cell cycle remains unclear.
- Research shows that different regions of the CENP-C protein are responsible for its centromere localization in interphase and mitosis, suggesting that CCAN organization changes dynamically as the cell cycle progresses.
Chromosomes Progress to Metaphase in Multiple Discrete Steps via Global Compaction/Expansion Cycles.
Cell
May 2015
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. Electronic address:
Mammalian mitotic chromosome morphogenesis was analyzed by 4D live-cell and snapshot deconvolution fluorescence imaging. Prophase chromosomes, whose organization was previously unknown, are revealed to comprise co-oriented sister linear loop arrays displayed along a single, peripheral, regularly kinked topoisomerase II/cohesin/condensin II axis. Thereafter, rather than smooth, progressive compaction as generally envisioned, progression to metaphase is a discontinuous process involving chromosome expansion as well as compaction.
View Article and Find Full Text PDFNeocentromeres.
Curr Biol
October 2014
Wellcome Trust Centre for Cell Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh, EH9 3JR, UK. Electronic address:
The centromere: chromatin foundation for the kinetochore machinery.
Dev Cell
September 2014
Wellcome Trust Centre for Cell Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh, EH9 3JR, UK. Electronic address:
Article Synopsis
- Centromeres are chromatin structures marked by the histone H3 variant CENP-A, crucial for assembling kinetochores rather than defined by specific DNA sequences.
- Recent studies, especially in vertebrates, reveal the importance of repetitive DNA sequences and various chromatin factors in the formation and activation of CENP-A chromatin for effective kinetochore assembly.
- The review highlights classic and recent research on centromeric chromatin, including the use of artificial chromosomes to better understand centromere function.