Quality Variation of Pork Bellies by Cutting Manner and Quality Grade.

In the meat industry, the quality grading system is commonly applied to classify carcasses based on quality and value. Presently, to facilitate consumer convenience, pork bellies are prepared into slices and retailed in supermarkets and butchers. The objective of this study was to assess the effect of quality grade (QG) and retail cutting manner on the quality properties of pork bellies.

Rapamycin treatment during prolonged maturation enhances the developmental competence of immature porcine oocytes.

Porcine oocytes undergo maturation (IVM) for 42-44 h. During this period, most oocytes proceed to metaphase and then to pro-metaphase if the nucleus has sufficiently matured. Forty-four hours is sufficient for oocyte nuclear maturation but not for full maturation of the oocyte cytoplasm.

Exosomes isolation from bovine serum: qualitative and quantitative comparison between ultracentrifugation, combination ultracentrifugation and size exclusion chromatography, and exoEasy methods.

Exosomes have been extensively studied as disease biomarker in humans, given their role in transporting bioactive molecules. However, despite the great potential of exosomes as noninvasive diagnostic markers and therapeutic nanocarriers for bovine diseases, few studies have been conducted on bovine exosome. Thus, this study aimed to quantitatively and qualitatively compare three isolation methods to identify a suitable method for bovine serum.

Differential gene expression in neonatal calf muscle tissues from Hanwoo cows overfed during mid to late pregnancy period.

Maternal nutrition significantly influences fetal development and postnatal outcomes. This study investigates the impact of maternal overfeeding during mid to late pregnancy on gene expression in the round and sirloin muscles of Hanwoo neonatal calves. Eight cows were assigned to either a control group receiving standard nutrition (100%) or a treated group receiving overnutrition (150%).

Pyrolytic conversion of cattle manure into value-added products and application of biochar for adsorption of sulfamethoxazole.

This study investigated the thermochemical conversion of cattle manure (CM) to propose a sustainable platform for its valorization, and explored the applicability of CM-derived biochar (CMB) as an environmental medium for the adsorptive removal of sulfamethoxazole (SMZ). CM pyrolysis was conducted under two atmospheric conditions (N and CO), and the pyrogenic products were quantified and characterized. Real-time syngas monitoring revealed that CO enhanced CO generation from the CM, leading to the formation of a highly porous carbon structure in the produced biochar (CMBCO).

Assessing the electrode configuration in a sandbox system for the removal of sulfanilamide: A pilot study.

Electrochemical oxidation has emerged as an effective and straightforward technology for groundwater remediation. While recent studies have investigated parameters such as current, electrolyte composition, and electrode materials, most have been conducted using small-scale batch or flow reactors, limiting their applicability to real-world conditions. In this study, a pilot-scale sandbox reactor was employed to simulate realistic groundwater conditions and assess the removal of sulfanilamide, a model organic contaminant.

Phytase, 25-hydroxyvitamin D and cocci vaccination to broilers fed a calcium and phosphorus-reduced diet under Eimeria spp. challenge: effects on growth performance and intestinal health.

A study evaluated the effects of phytase, 25-hydroxyvitamin D (25OHD), and cocci vaccination on broilers fed a diet reduced in calcium (Ca) and available phosphorus (avP) under Eimeria challenge. A total of 840 one-day-old male broilers were assigned to a 2 × 5 factorial arrangement based on cocci vaccination and dietary treatments. Half of the birds were vaccinated against coccidia on d 1, and all birds were orally challenged with Eimeria spp.

The Influence of Specific Pathogen-Free and Conventional Environments on the Hematological Parameters of Pigs Bred for Xenotransplantation.

Blood analysis plays a pivotal role in assessing the health of laboratory animals, including pigs. This study investigated the hematological profiles of transgenic pigs of the MGH breed for xenotransplantation, focusing on the effect of housing conditions on blood parameters. A cohort of pigs was longitudinally monitored from 6 to 18 months of age in both conventional and specific pathogen-free (SPF) environments.

Development of the PD9-9 Monoclonal Antibody for Identifying Porcine Bone Marrow-Derived Dendritic Cells.

The purpose of this study was to develop a monoclonal antibody (mAb) that can identify porcine dendritic cells (DCs) that have differentiated from bone marrow progenitor cells. Hybridoma technology was used to obtain mAbs, and bone marrow-derived DCs (BMDCs) were employed as immunogens for producing antibodies. The generated PD9-9 mAbs exhibited considerable reactivity towards porcine BMDCs with applications in flow cytometry and immunostaining.

Identification of Reference Gene for Quantitative Gene Expression in Early-Term and Late-Term Cultured Canine Fibroblasts Derived from Ear Skin.

Fibroblasts are cells that reside within the fibrous or loose connective tissues of most mammalian organs. For research purposes, fibroblasts are often subjected to long-term culture under defined conditions, during which their properties can significantly change. It is essential to understand and document these changes to obtain reliable outcomes.

Effects of Root on In Vitro and In Vivo Methane Production and Rumen Fermentation Characteristics.

This study investigated the impact of root (RP) for reducing methane and its impact on rumen fermentation and blood metabolites in cattle. Rumen fluid was collected from three cannulated steers (736 ± 15 kg) and mixed with buffer (1:3 ratio) for the in vitro trial. Treatments were divided into control and RP supplement groups (1%, 3%, and 5% of substrates), with each sample incubated at 39 °C for 24 and 48 hours.

Infrared thermometry for detecting estrus and pregnancy in Holstein cows.

Efficient reproductive management is paramount in enhancing the productivity and welfare of dairy cows. This study investigates the effects of pregnancy status, seasonal variations, and diurnal shifts on the body temperature of different body parts in dairy cows. Using a structured approach, cows were categorized based on pregnancy status (pregnant vs.

Sustainable organic waste valorisation: A zero-waste approach.

The annual increase in global organic waste generation emphasises the need to develop a sustainable management platform to address environmental concerns. This study aims to explore sustainable treatments for the conversion of organic waste into energy in pursuit of zero-waste. The organic waste generated from the animal feed industry (referred to as WF) was used for the model compound in this study.

Optimization of donor cell production for somatic cell nuclear transfer in the critically endangered Vietnamese Ỉ pig.

We aimed to establish efficient donor cells to produce piglets by somatic cell nuclear transfer (SCNT) of the endangered Vietnamese Ỉ pig. In Experiment 1, we assessed the effects of cell passages on the in vitro development of SCNT embryos. Cells with five and six passages showed significantly cleaved and blastocyst formation rates (86.

Effects of Short-Term Differences in Concentrate Feeding on the Recovery of In Vivo Embryos in Hanwoo Donor Cows through Superovulation Treatment.

Superovulation is a technique used to increase the number of oocytes released for fertilization. This study investigated the effects of short-term differences in concentrate feed intake on in vivo embryo production through superovulation in indigenous Korean (Hanwoo) cows. The cows were given fresh water and hay ad libitum and randomly divided into three groups (control (CON, n = 9): 2.

Heat Stress Induces Alterations in Gene Expression of Actin Cytoskeleton and Filament of Cellular Components Causing Gut Disruption in Growing-Finishing Pigs.

We aimed to investigate the impact of heat stress (HS) on the expression of tight junction (TJ) proteins and the interaction between genes affecting intestinal barrier function using transcriptomics in the porcine jejunum. Twenty-four barrows (crossbred Yorkshire × Landrace × Duroc; average initial body weight, 56.71 ± 1.

Establishment of circular economy by utilising textile industry waste as an adsorbent for textile dye removal.

This study explored the use of waste from the textile industry (silkworm byproducts) as a promising raw feedstock for the production of carbon-based adsorbents (biochar). The silk excreta biochar generated at 600 and 700 °C (referred to as SEB-600 and SEB-700, respectively) were evaluated in terms of their efficacy in adsorbing cationic (methylene blue) and anionic (Congo red) textile dyes. Although the functional groups on the surfaces of SEB-600 and SEB-700 were not significantly different, the specific surface area of SEB-700 was greater than that of SEB-600.

Anti-obesity and immunomodulatory effects of Allium hookeri leaves cultivated with artificial light of different intensities on immune-depressed obese mice.

This study was conducted to evaluate the effects of Allium hookeri (AH) leaves cultivated with different light-emitting diode (LED) intensities (L: low, 100 μmol/m/s; M: medium, 150 μmol/m/s; H: high, 200 μmol/m/s). Alliin concentration increased as light intensity increased in AH and showed the highest level at LED-H condition. The anti-obesity and immunomodulatory properties of AH were evaluated in a cyclophosphamide (CPA)-induced immunosuppressed obese animal model.

Comparison of Muscle Fiber and Meat Quality Characteristics of Beef Strip Loin, Tenderloin, and Round Cuts among Jeju Black Cattle, Hanwoo, and Their Crossbreeds.

This study assessed and compared meat quality and fiber characteristics of (LL), (PM), and muscles among Hanwoo (HW), Jeju black (BL), and their crossbred (BH) cattle. Twelve carcasses from each breed (36 in total) were used in this study. BL and BH had higher moisture and crude ash contents and lower crude fat and protein contents than HW, regardless of the muscle type.

In vitro maturation using porcine follicular fluid-derived exosomes as an alternative to the conventional method.

Extracellular vesicles, also known as exosomes, influence numerous cellular functions by regulating different signaling pathways. However, their role in animal reproduction remains understudied. This study aimed to evaluate the effects of porcine follicular fluid-derived exosomes (pff-Exos) on porcine oocyte in vitro maturation and parthenogenetic embryo development.

Improved surrogate eggshell incubation system for assisted reproductive technologies such as cloning, genome modification, and de-extinction efforts.

A germinal disc located on the egg yolk surface drives genetic modification. Windowed and surrogate eggshell incubation methods have been developed, but these exhibit limited abilities to generate transgenic chickens. In the present study, we investigated the frequency of observing the germinal disc according to the preincubation positioning direction and time and found that it depended on those conditions, but only a few chicks (2.