36 results match your criteria: "National Institute for Agricultural and Food Research and Technology (INIA-CSIC)[Affiliation]"

Article Synopsis
  • * Studied how testosterone influenced sperm quality during cryopreservation, finding that it negatively impacted acrosome integrity in mouflons and ibexes, especially when combined with the aquaporin blocker phloretin.
  • * Concluded that testosterone and the AQP blocker adversely affect sperm cryoresistance, contributing to an understanding of seasonal changes in sperm freezing capacity in ruminants.
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The herbicide profoxydim, which is chiral in nature, is commonly used for weed control in rice. However, traditional achiral analysis treats profoxydim isomers as a single compound, providing only partial and inaccuracy information assuming identical behavior for the four isomers. To address this issue, a sensitive and straightforward chiral analytical method was developed using QuEChERS and chiral HPLC-MS/MS to determine the residues of all four stereoisomers of profoxydim in soil.

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The study focused on the photodegradation of profoxydim, a low-toxicity cyclohexanedione herbicide commonly used in rice crops, under simulated sunlight conditions. Profoxydim's behavior in paddy field conditions is not well understood, and this research aimed to fill that gap, particularly examining the effect of commonly utilized organic amendments such as biochar (BC) on its degradation. Results indicated that profoxydim degrades rapidly, with a half-life of 2.

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Effect of Commercial Trap Design and Location on Captures of (Fabricius) (Coleoptera: Dryophthoridae) on Palm Trees.

Insects

September 2024

Unidad de Protección Vegetal, Instituto Canario de Investigaciones Agrarias (ICIA), Crta. El Boquerón, s/n, 38270 La Laguna, Spain.

(Fabricius) (Coleoptera: Dryophthoridae) is a weevil present in the Canary Islands, affecting economically important palms such as H. Wildpret and its hybrids, for which there were no trapping tools. The larvae cause the main damage by burrowing galleries in the rachis of the leaves, causing premature drying and collapse.

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Article Synopsis
  • - This study examined ways to improve assisted reproductive techniques for conserving wild cats, using domestic cats as a reference.
  • - Researchers tested three types of in vitro culture media to see how they influenced the formation of blastocysts, finding similar success rates of over 16%.
  • - The study shows promise for these reproductive methods but suggests more research is needed to assess embryonic quality and enhance protocols for conserving wild felids.
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This study investigates the impact of bovine serum albumin (BSA) inclusion in the thawing extender on boar sperm quality. Thawing protocols in sperm cryopreservation are vital, yet underexplored. It has been determined that BSA can interact with membranes, stabilizing them and preventing damage during the freezing process, for this reason it could also have a beneficial effect during thawing.

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Corrigendum: sp. nov., a novel fructophilic species isolated from flowers of .

Int J Syst Evol Microbiol

September 2024

Laboratorio de Bacterias Lácticas y Probióticos, Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Av. Agustín Escardino 7, 46980 Paterna, Spain.

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Cryopreservation of porcine spermatozoa is detrimental due to their high sensitivity to cold shock, leading to changes akin to capacitation, known as cryocapacitation. These changes, including the acrosomal reaction, hypermotility induction, and protein phosphorylation, might be influenced by the presence of progesterone in seminal plasma and egg yolk, used in most freezing extenders. We tested the effect of various progesterone concentrations added to the freezing extenders (1, 10, and 100 μg/mL).

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sp. nov., a novel fructophilic species isolated from flowers of .

Int J Syst Evol Microbiol

August 2024

Laboratorio de Bacterias Lácticas y Probióticos, Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Av. Agustín Escardino 7, 46980 Paterna, Spain.

A survey of fructophilic lactic acid bacteria associated with wild and cultivated plants in the metropolitan area of Valencia (Spain) led to the isolation of a novel strain of the genus , named Es01, from flowers of . The genus encompasses a single species, . Partial 16S rRNA coding gene sequencing revealed a similarity of 98.

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Global citrus root microbiota unravels assembly cues and core members.

Front Microbiol

July 2024

Citrus Research and Education Center, Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences (IFAS), University of Florida, Lake Alfred, FL, United States.

Introduction: Citrus is one of the most important fruit crops worldwide, and the root-associated microbiota can have a profound impact on tree health and growth.

Methods: In a collaborative effort, the International Citrus Microbiome Consortium investigated the global citrus root microbiota with samples collected from nine citrus-producing countries across six continents. We analyzed 16S rDNA and ITS2 amplicon sequencing data to identify predominant prokaryotic and fungal taxa in citrus root samples.

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Theoretical Study of Structural and Electronic Trends of the Sulfonylurea Herbicides Family.

J Phys Chem A

July 2024

Departamento de Química, Facultad de Ciencias, Módulo 13, Universidad Autónoma de Madrid, 28049 Madrid, Spain.

The sulfonylurea herbicide family has been extensively studied using computational techniques. The most stable conformer structures of the 34 molecules analyzed in gaseous, aqueous, and octanol phases have been determined. The study employed CREST conformational search methods along with the CENSO script to explore all possible conformational structures.

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The conservation of genetic resources in pig breeds, notably the Iberian pig, is crucial for genetic improvement and sustainable production. Prolonged storage in liquid nitrogen (LN) is recognized for preserving genetic diversity, but potential adverse effects on seminal quality remain debated. This study aims to assess the impact of ten years of storage at different LN levels and to optimize thawing protocols for Iberian pig sperm.

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Most of the previous studies on the genetic variability in Spanish "Berrenda" breeds have been carried out using DNA microsatellites. The present work aimed to estimate the genetic diversity, population structure, and potential genetic differences among individuals of both Berrenda breeds and groups based on the presence of the Robertsonian chromosomal translocation, rob (1;29). A total of 373 samples from animals belonging to the two breeds, including 169 cases diagnosed as rob (1;29)-positive, were genotyped using an SNP50K chip.

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A method for the simultaneous determination of the four stereoisomers of the chiral herbicide profoxydim in rice and husk was developed using the QuEChERS method and LC-tandem mass spectrometry. Four polysaccharide-based chiral stationary phase columns were evaluated. All four stereoisomers were successfully separated on a Chiracel OJ-3R column.

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MicroRNA expression in specific segments of the pig periovulatory internal genital tract is differentially regulated by semen or by seminal plasma.

Res Vet Sci

March 2024

Department of Biomedical & Clinical Sciences (BKV), BKH/Obstetrics & Gynecology, Faculty of Medicine and Health Sciences, Linköping University, 58185 Linköping, Sweden. Electronic address:

microRNAs play pivotal roles during mammalian reproduction, including the cross-talk between gametes, embryos and the maternal genital tract. Mating induces changes in the expression of mRNA transcripts in the female, but whether miRNAs are involved remains to be elucidated. In the current study, we mapped 181 miRNAs in the porcine peri-ovulatory female reproductive tract: Cervix (Cvx), distal and proximal uterus (Dist-Ut, Prox-Ut), Utero-tubal-junction (UTJ), isthmus (Isth), ampulla (Amp), and infundibulum (Inf) when exposed to semen (natural mating (NM) or artificial insemination (AI-P1)) or to infusions of sperm-free seminal plasma (SP): the first 10 mL of the sperm rich fraction (SP-P1) or the entire ejaculate (SP-E).

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This study describes the characterization of the recombinant GH3 aryl-β-glucosidase "GluLm" from Limosilactobacillus mucosae INIA P508, followed by its immobilization on an agarose support with the aim of developing an efficient application to increase the availability and concentration of flavonoid and lignan aglycones in a vegetal beverage. In previous studies, heterologous GluLm-producing strains demonstrated a great capacity to deglycosylate flavonoids. Nevertheless, the physicochemical properties and substrate spectrum of the enzyme remained unknown up to now.

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Some lactic acid bacteria (LAB) have the ability to synthesize riboflavin, a trait linked to the presence of ribG, ribB, ribA and ribH genes located in the rib operon. Previous screening of riboflavin producers identified several LAB strains belonging to different species with this ability, but none of them surpassed 0.25 mg/L production of the vitamin.

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The fate and behavior of herbicides can be altered in an unpredictable way when organic amendments are added to soil as a beneficial management tool. The objective of this work was to investigate the effect exerted by the addition of two different organic amendments (alperujo compost and biochar) to soil in the degradation of one of the most relevant new generation rice herbicides, profoxydim. In unamended soils, the degradation of profoxydim was quite fast and was governed by both chemical (DT50 = from 1.

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Sperm capacitation is a crucial step towards the acquisition of fertilizing capacity. Despite the attempts to mimic the in vivo situation, there is still a lack of standardization in vitro techniques. Bicarbonate and serum albumin (BSA) are routinely used, although controversial results are reported regarding the optimal concentration of each compound.

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Modulation of Polar Auxin Transport Identifies the Molecular Determinants of Source-Sink Carbon Relationships and Sink Strength in Poplar.

Tree Physiol

June 2023

Environmental Molecular Sciences Laboratory (EMSL), Pacific Northwest National Laboratory (PNNL), Richland, Washington  USA.

Source-to-sink carbon (C) allocation driven by the sink strength, i.e., the ability of a sink organ to import C, plays a central role in tissue growth and biomass productivity.

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Tracking nanoplastics in freshwater microcosms and their impacts to aquatic organisms.

J Hazard Mater

March 2023

Department of Biology, Faculty of Science, Universidad Autónoma de Madrid, E-28049 Madrid, Spain. Electronic address:

In this work, we used palladium-doped polystyrene NPLs (PS-NPLs with a primary size of 286 ± 4 nm) with an irregular surface morphology which allowed for particle tracking and evaluation of their toxicity on two primary producers (cyanobacterium, Anabaena sp. PCC7120 and green algae, Chlamydomonas reinhardtii) and one primary consumer (crustacean, Daphnia magna). the concentration range for Anabaena and C.

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Some lactic acid bacteria (LAB) strains have the ability to synthesize riboflavin, a trait linked to the presence of ribG, ribB, ribA and ribH genes in the rib operon. Multiple sequence alignments of these genes showed that these sequences are not identical in different LAB species, so primers designed to detect these genes in one species do not always work with others. Therefore, we designed degenerate primers based on sequences from Lactococcus lactis MG1363, Levilactobacillus brevis ATCC 367 and Limosilactobacillus fermentum IFO3956, and established optimal PCR conditions for the detection of rib genes in different LAB species.

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Fungal Pathogens and Seed Storage in the Dry State.

Plants (Basel)

November 2022

Department of Agricultural Production, School of Agricultural, Food and Biosystems Engineering, Universidad Politécnica de Madrid, Avda. Puerta de Hierro, 4, 28040 Madrid, Spain.

Seeds can harbor a wide range of microorganisms, especially fungi, which can cause different sanitary problems. Seed quality and seed longevity may be drastically reduced by fungi that invade seeds before or after harvest. Seed movement can be a pathway for the spread of diseases into new areas.

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The search for criteria that allow the quantification of the level of thermotolerance of an animal is a major challenge in animal production. Different criteria have been proposed to date, mainly the use of routine milk recording and weather information or the collection of physiological measures related with heat stress. This study aimed at quantifying the association between indicators of heat tolerance derived from productive and physiological traits.

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The number of published studies evaluating the effects of microplastics (MPs) in fish has increased in the last decade. However, of the available studies, few have explored the long-term effects of MPs on fish growth and reproduction and have resorted to MPs in the form of μm-sized beads/microspheres. In this study, 6-10 day-old post-hatch medaka (Oryzias latipes) fish were exposed to 50 (i.

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