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Repair of an Attenuated Low-Passage Murine Cytomegalovirus Bacterial Artificial Chromosome Identifies a Novel Spliced Gene Essential for Salivary Gland Tropism.
J Virol
October 2020
SpeedX Pty Ltd., National Innovative Centre, Evenleigh, New South Wales, Australia.
Article Synopsis
- The study successfully cloned a low-passage strain of murine cytomegalovirus (MCMV) known as G4 as a bacterial artificial chromosome (BAC), enabling better manipulation and understanding of the virus.
- Unlike lab strains, G4 can replicate effectively in C57BL/6 mice due to a specific gene modification (m157) that alters its interaction with natural killer (NK) cells.
- Through next-generation sequencing, researchers discovered a mutation affecting salivary gland tropism and identified a novel spliced gene (sgg1.1) whose restoration improved the virus's ability to infect salivary glands, offering new insights into the virus's biology.