Integration of Transcriptome and Methylome Analyses Provides Insight Into the Pathway of Floral Scent Biosynthesis in .

DNA methylation is a common epigenetic modification involved in regulating many biological processes. However, the epigenetic mechanisms involved in the formation of floral scent have rarely been reported within a famous traditional ornamental plant emitting pleasant fragrance in China. By combining whole-genome bisulfite sequencing and RNA-seq, we determined the global change in DNA methylation and expression levels of genes involved in the biosynthesis of floral scent in four different flowering stages of .

Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis.

MYBs ( avian myeloblastosis viral oncogene homologs) are important transcriptional regulators that play critical roles in the regulation of anthocyanin biosynthesis. The overexpression of genes has been reported in different plant species. However, the inconsistent strategies to assess transgenic plants have made it difficult to explain the complex mechanisms of regulation of anthocyanin biosynthesis by .

Identification of trehalose-6-phosphate synthase (TPS)-coding genes involved in flowering induction of Lilium× formolongi.

Lilium × formolongi, a facultative long-day (LD) plant, can complete the floral transition within one year after sowing under LD conditions. In addition to the photoperiod, the molecular mechanisms by which other flowering regulators, such as sugar, participate in juvenile development and flowering induction in L. × formolongi remain elusive.

[Accumulation, subcellular distribution, and chemical forms of zinc in three tree species].

In order to explore the mechanism underlying zinc (Zn) accumulation and tolerance in woody garden species, the effects of different Zn concentrations (0, 250, 500, 1000, 2000 mg·kg) on leaf, branch, root biomass and leaf ultrastructure of , , and were studied in a pot pollution simulation experiment. The concentration of Zn in plant organs, the subcellular distribution and chemical forms of Zn in leaves and roots were further analyzed. The results showed that all the three species could survive under diffe-rent Zn concentrations, but the biomass of leaves, stems and roots decreased compared with the control.

Hormonal Regulatory Patterns of LaKNOXs and LaBEL1 Transcription Factors Reveal Their Potential Role in Stem Bulblet Formation in LA Hybrid Lily.

In lily reproduction, the mechanism of formation of bulbs has been a hot topic. However, studies on stem bulblet formation are limited. Stem bulblets, formed in the leaf axils of under- and above-ground stems, provide lilies with a strong capacity for self-propagation.

Cryptochrome 2 from Regulates Photoperiodic Flowering in Transgenic .

The photoperiodic flowering pathway is essential for plant reproduction. As blue and ultraviolet-A light receptors, cryptochromes play an important role in the photoperiodic regulation of flowering. is an important cut flower that flowers within a year after seed propagation.

Molecular Characterization and Expression Patterns of the Gene Reveal Distinct Roles in Inflorescence Structure and Floral Organ Development in .

() genes are members of the well-known MADS-box gene family that play a key role in regulating vital developmental processes in plants. are perennial herbs that exhibit continuous flowering development and have been extensively used in landscaping. However, there are few reports on the regulatory mechanism of flowering in .

ScGST3 and multiple R2R3-MYB transcription factors function in anthocyanin accumulation in Senecio cruentus.

Anthocyanins are important flavonoid pigments involved in the colouring of flowers and fruits. They are synthesized on the cytoplasmic surface of the endoplasmic reticulum and transported into the vacuole for storage. Previous reports have suggested that glutathione S-transferase (GST) is involved in anthocyanin transport.

Genome-Wide Identification and Low-Temperature Expression Analysis of bHLH Genes in .

is an illustrious ornamental woody plant with colorful flowers, delicate fragrances, and graceful tree forms. Low temperature limits its geographical distribution. The basic helix-loop-helix (bHLH) proteins exist in most eukaryotes as a transcription factor superfamily, which play a crucial role in metabolism, physiology, development, and response to various stresses of higher organisms.

Decapitation Experiments Combined with the Transcriptome Analysis Reveal the Mechanism of High Temperature on Chrysanthemum Axillary Bud Formation.

Temperature is an important factor that largely affects the patterns of shoot branching in plants. However, the effect and mechanism of temperature on axillary bud development in chrysanthemum remains poorly defined. The purpose of the present study is to investigate the effect of high temperature on the axillary bud growth and the mechanism of axillary bud formation in chrysanthemum.

Dissection of Allelic Variation Underlying Floral and Fruit Traits in Flare Tree Peony () Using Association Mapping.

Allelic variation in floral quantitative traits, including the elements of flowers and fruits, is caused by extremely complex regulatory processes. In the genetic improvement of flare tree peony (), a unique ornamental and edible oil woody species in the genus , a better understanding of the genetic composition of these complex traits related to flowers and fruits is needed. Therefore, we investigated the genetic diversity and population structure of 160 accessions and conducted single-marker association analysis for 19 quantitative flower and fruit traits using 81 EST-SSR markers.

Stomata and ROS changes during Botrytis elliptica infection in diploid and tetraploid Lilium rosthornii Diels.

Polyploid plants often show improved resistance against many diseases, but whether they show increased resistance to grey mould, a devastating disease caused by Botrytis spp. fungi, is seldom reported. Stomata and reactive oxygen species (ROS) play dual roles in defence against grey mould, and it is unclear how their roles change after polyploidization.

Comparative Transcriptome and Weighted Gene Co-expression Network Analysis Identify Key Transcription Factors of 'Old Blush' After Exposure to a Gradual Drought Stress Followed by Recovery.

Rose is one of the most fundamental ornamental crops, but its yield and quality are highly limited by drought. The key transcription factors (TFs) and co-expression networks during rose's response to drought stress and recovery after drought stress are still limited. In this study, the transcriptomes of leaves of 2-year-old cutting seedlings of 'Old Blush' from three continuous droughted stages (30, 60, 90 days after full watering) and rewatering were analyzed using RNA sequencing.

Integrating Genome-Wide Association Analysis With Transcriptome Sequencing to Identify Candidate Genes Related to Blooming Time in .

is one of the most important woody perennials for edible and ornamental use. Despite a substantial variation in the flowering phenology among the germplasm resources, the genetic control for flowering time remains to be elucidated. In this study, we examined five blooming time-related traits of 235 landraces for 2 years.

Integrative Identification of Crucial Genes Associated With Plant Hormone-Mediated Bud Dormancy in .

is an important ornamental woody plant with winter-flowering property, which is closely related to bud dormancy. Despite recent scientific headway in deciphering the mechanism of bud dormancy in , the overall picture of gene co-expression regulating bud dormancy is still unclear. Here a total of 23 modules were screened by weighted gene co-expression network analysis (WGCNA), of which 12 modules were significantly associated with heteroauxin, abscisic acid (ABA), and gibberellin (GA), including GA1, GA3, and GA4.

The complete chloroplast genome of .

(Decne.) Rgl. 1879 (family Asteraceae) is a perennial herb with high economic and medicinal values.

A highly efficient regeneration, genetic transformation system and induction of targeted mutations using CRISPR/Cas9 in Lycium ruthenicum.

Background: CRISPR/Cas9 is a rapidly developing genome editing technology in various biological systems due to its efficiency, portability, simplicity and versatility. This editing technology has been successfully applied in in several important plants of Solanaceae such as tomato, tobacco, potato, petunia and groundcherry. Wolfberry ranked the sixth among solanaceous crops of outstanding importance in China following potato, tomato, eggplant, pepper and tobacco.

Identification and QTL Analysis of Flavonoids and Carotenoids in Tetraploid Roses Based on an Ultra-High-Density Genetic Map.

Roses are highly valuable within the flower industry. The metabolites of anthocyanins, flavonols, and carotenoids in rose petals are not only responsible for the various visible petal colors but also important bioactive compounds that are important for human health. In this study, we performed a QTL analysis on pigment contents to locate major loci that determine the flower color traits.

Identification and analysis of NAC transcription factors and an expression analysis of OsNAC7 subfamily members.

NAC (NAM, ATAF1-2, and CUC2) transcription factors (TFs) play a vital role in plant growth and development, as well as in plant response to biotic and abiotic stressors (Duan et al., 2019; Guerin et al., 2019).

Functional characterization of cold-responsive Zinc Finger Homeodomain () gene in abscisic acid and osmotic stress tolerance.

Background: We have previously performed an analysis of the cold-responsive transcriptome in the mature leaves of tiger lily () by gene co-expression network identification. The results has revealed that a gene, notated as encoding zinc finger homeodomain protein, may play an essential regulating role in tiger lily response to cold stress.

Methods: A further investigation of the gene (termed as ) responding to osmotic stresses, including cold, salt, water stresses, and abscisic acid (ABA) was performed in this study.

Integrated mRNA and small RNA sequencing reveals a regulatory network associated with flower color in oriental hybrid lily.

Anthocyanins are one of the main components of pigments, that are responsible for a wide range of colors in plants. To clarify the regulatory mechanism of anthocyanin biosynthesis in oriental hybrid lily, UPLC/MS analysis was performed to identify the pigments in two cultivars (white and pink). Four major anthocyanins were identified in pink cultivar, and no anthocyanins were detected in white cultivar.

Overexpression d-Type Cyclin Gene Influences Cell Division and Produces Curved Leaf in .

d-type cyclins (CYCDs) are a special class of cyclins and play extremely important roles in plant growth and development. In the plant kingdom, most of the existing studies on CYCDs have been done on herbaceous plants, with few on perennial woody plants. Here, we identified a  d-type cyclin gene, , which is mainly transcribed in leaf buds and stems.

Identification of the PmWEEP locus controlling weeping traits in Prunus mume through an integrated genome-wide association study and quantitative trait locus mapping.

Weeping Prunus mume (mei) has long been cultivated in East Asia for its specific ornamental value. However, little is known about the regulatory mechanism of the weeping trait in mei, which limits molecular breeding for the improvement of weeping-type cultivars. Here, we quantified the weeping trait in mei using nested phenotyping of 214 accessions and 342 F hybrids.

Analysis of miRNA-mediated regulation of flowering induction in Lilium × formolongi.

Background: MicroRNAs play pivotal roles in plant vegetative phase change and flowering induction via integrating into multiple flowering pathways. Lilium × formolongi is an important ornamental lily cultivar that can flower within one year after sowing. However, it remains unresolved how miRNA-mediated regulation networks contribute to the L.