Dry Bondable Porous Silk Fibroin Films for Embedding Micropatterned Electronics in Hierarchical Silk Nacre.

Future structural materials is not only be lightweight, strong, and tough, but also capable of integrating functions like sensing, adaptation, self-healing, deformation, and recovery as needed. Although bio-inspired materials are well developed, directly integrating microelectronic patterns into nacre-mimetic structures remains challenging, limiting the widespread application of electronic biomimetic materials. Here, an in situ freeze-drying method is reported for the successful preparation of porous silk fibroin materials that can achieve dry bonding.

Interaction between walkability and fine particulate matter on ischemic heart disease: A prospective cohort study in China.

Background: Previous studies have suggested that neighborhoods characterized by higher walkability are related to a reduced risk of ischemic heart disease (IHD), whereas exposure to PM is positively associated with risk of IHD. Nevertheless, their joint impact on IHD warrants further investigation.

Methods: This prospective cohort study was performed in Yinzhou, Ningbo, China, comprising 47,516 participants.

Dynamic and quantitative assessment of quercetin for cardiac oxidative stress injury prevention using sensitive cardiomyocyte based biosensing.

Myocardial infarction is a leading cause of morbidity and mortality associated with cardiovascular diseases worldwide. Although novel medications and treatments greatly alleviate patient suffering, challenges related to prognostic limit the recovery of cardiac function. Currently, treatment with monomeric compounds displays promise in prognostic interventions for cardiac diseases.

The association between family socio-demographic factors, parental mediation and adolescents' digital literacy: a cross-sectional study.

Background: Digital technology has a two-sided impact whether it is used appropriately or not. It is crucial to promote the benefits of digital technology and reduce its harms by bolstering adolescents' digital literacy. The appropriateness of the strategies parents use to manage their children's digital lives plays a key role.

Energy stress induced cardiac autophagy detection via a chronic and dynamic cardiomyocytes-based biosensing platform.

Hypoglycemia is a common complication which occurs during the treatment of diabetes, closely associated with cardiovascular events. A sudden decrease in blood glucose increases the risk of arrhythmia, which can lead to sudden cardiac death. This event is usually accompanied by abnormal electrophysiological activities in cardiomyocytes.

Outdoor light at night, air pollution and risk of incident type 2 diabetes.

Background: Air pollution and outdoor light at night (LAN) have been reported to be related to type 2 diabetes (T2D). However, their interaction with risk of T2D remains uncertain. Therefore, our study aimed to explore the relationship between outdoor LAN, air pollution and incident T2D.

Deep learning permits imaging of multiple structures with the same fluorophores.

Fluorescence microscopy, which employs fluorescent tags to label and observe cellular structures and their dynamics, is a powerful tool for life sciences. However, due to the spectral overlap between different dyes, a limited number of structures can be separately labeled and imaged for live-cell applications. In addition, the conventional sequential channel imaging procedure is quite time consuming, as it needs to switch either different lasers or filters.

Scalable Drug-Mimicking Nanoplasmonic Therapy for Bradyarrhythmia in Cardiomyocytes.

Bradyarrhythmia poses a serious threat to human health, with chronic progression causing heart failure and acute onset leading to sudden death. In this study, we develop a scalable drug-mimicking nanoplasmonic therapeutic strategy by introducing gold nanorod (Au NR) mediated near-infrared (NIR) photothermal effects. An integrated sensing and regulation platform is established for in situ synchronized NIR laser regulation and electrophysiological property recording.

Optogenetic Strategies for Optimizing the Performance of Phospholipids Biosensors.

High-performance biosensors play a crucial role in elucidating the intricate spatiotemporal regulatory roles and dynamics of membrane phospholipids. However, enhancing the sensitivity and imaging performance remains a significant challenge. Here, optogenetic-based strategies are presented to optimize phospholipid biosensors.

An Integrated Optogenetic and Bioelectronic Platform for Regulating Cardiomyocyte Function.

Bioelectronic medicine is emerging as a powerful approach for restoring lost endogenous functions and addressing life-altering maladies such as cardiac disorders. Systems that incorporate both modulation of cellular function and recording capabilities can enhance the utility of these approaches and their customization to the needs of each patient. Here we report an integrated optogenetic and bioelectronic platform for stable and long-term stimulation and monitoring of cardiomyocyte function in vitro.

A dual-mode, image-enhanced, miniaturized microscopy system for incubator-compatible monitoring of live cells.

Imaging live cells under stable culture conditions is essential to investigate cell physiological activities and proliferation. To achieve this goal, typically, a specialized incubation chamber that creates desired culture conditions needs to be incorporated into a microscopy system to perform cell monitoring. However, such imaging systems are generally large and costly, hampering their wide applications.

Near-infrared-triggered plasmonic regulation and cardiomyocyte-based biosensing system for in vitro bradyarrhythmia treatment.

Bradyarrhythmia, a life-threatening cardiovascular disease, is an increasing burden for the healthcare system. Currently, surgery, implanted device, and drug are introduced to treat the bradyarrhythmia in clinical practice. However, these conventional therapeutic strategies suffer from the invasive surgery, power supply, or drug side effect, respectively, hence developing the alternative therapeutic strategy is necessarily imperative.

PRL1 and PRL3 promote macropinocytosis via its lipid phosphatase activity.

PRL1 and PRL3, members of the protein tyrosine phosphatase family, have been associated with cancer metastasis and poor prognosis. Despite extensive research on their protein phosphatase activity, their potential role as lipid phosphatases remains elusive. We conducted comprehensive investigations to elucidate the lipid phosphatase activity of PRL1 and PRL3 using a combination of cellular assays, biochemical analyses, and protein interactome profiling.

Micronano Synergetic Three-Dimensional Bioelectronics: A Revolutionary Breakthrough Platform for Cardiac Electrophysiology.

Cardiovascular diseases (CVDs) are the leading cause of mortality and therefore pose a significant threat to human health. Cardiac electrophysiology plays a crucial role in the investigation and treatment of CVDs, including arrhythmia. The long-term and accurate detection of electrophysiological activity in cardiomyocytes is essential for advancing cardiology and pharmacology.

Deep learning-enabled fast DNA-PAINT imaging in cells.

DNA-based point accumulation in nanoscale topography (DNA-PAINT) is a well-established technique for single-molecule localization microscopy (SMLM), enabling resolution of up to a few nanometers. Traditionally, DNA-PAINT involves the utilization of tens of thousands of single-molecule fluorescent images to generate a single super-resolution image. This process can be time-consuming, which makes it unfeasible for many researchers.

Active Micro-Nano-Collaborative Bioelectronic Device for Advanced Electrophysiological Recording.

The development of precise and sensitive electrophysiological recording platforms holds the utmost importance for research in the fields of cardiology and neuroscience. In recent years, active micro/nano-bioelectronic devices have undergone significant advancements, thereby facilitating the study of electrophysiology. The distinctive configuration and exceptional functionality of these active micro-nano-collaborative bioelectronic devices offer the potential for the recording of high-fidelity action potential signals on a large scale.

A cardiomyocyte-based biosensing platform for dynamic and quantitative investigation of excessive autophagy.

Autophagy is an important physiological phenomenon in eukaryotes that helps maintain the cellular homeostasis. Autophagy is involved in the development of various cardiovascular diseases, affecting the maintenance of cardiac function and disease prognosis. Physiological levels of autophagy serve as a defense mechanism for cardiomyocytes against environmental stimuli, but an overabundance of autophagy may contribute to the development of cardiovascular diseases.

Weight change and all-cause and cause-specific mortality: A 25-year follow-up study.

Background: Whether the dynamic weight change is an independent risk factor for mortality remains controversial. This study aimed to examine the association between weight change and risk of all-cause and cause-specific mortality based on the Linxian Nutrition Intervention Trial (NIT) cohort.

Methods: Body weight of 21,028 healthy residents of Linxian, Henan province, aged 40-69 years was measured two times from 1986 to 1991.

Interaction between plant-based dietary pattern and frailty on cognitive decline: a longitudinal analysis of the Chinese Longitudinal Healthy Longevity Survey cohort.

Objectives: Frailty is a risk factor for faster cognitive decline, while plant-based dietary patterns are associated with decreased risk of cognitive decline. We aimed to explore their interaction with cognitive function among older adults.

Methods: We used data from the Chinese Longitudinal Healthy Longevity Survey between 2008 and 2018.

Association between visit-to-visit lipid variability and risk of ischemic heart disease: a cohort study in China.

Aims: To explore the associations between visit-to-visit lipid variability and risk of ischemic heart disease (IHD) in a population-based cohort in China.

Methods: We evaluated lipid variability in 30,217 individuals from the Yinzhou Health Information System who had ≥3 recorded lipid measurements during 2010-2014. We used various indicators including standard deviation (SD), coefficient of variation (CV), variability independent of the mean (VIM), and average real variability (ARV) to quantify the variability in triglycerides, total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C).

Electroacupuncture prevents the development or establishment of chronic pain via IL-33/ST2 signaling in hyperalgesic priming model rats.

Background: Chronic pain is acomplexhealth issue. Compared to acute pain, which has a protective value, chronic pain is defined as persistent pain after tissue injury. Few clinical advances have been made to prevent the transition from acute to chronic pain.

Boosting microscopic object detection via feature activation map guided poisson blending.

Microscopic examination of visible components based on micrographs is the gold standard for testing in biomedical research and clinical diagnosis. The application of object detection technology in bioimages not only improves the efficiency of the analyst but also provides decision support to ensure the objectivity and consistency of diagnosis. However, the lack of large annotated datasets is a significant impediment in rapidly deploying object detection models for microscopic formed elements detection.