The 2023 fatal dengue outbreak in Bangladesh highlights a paradigm shift of geographical distribution of cases.

In 2023, Bangladesh experienced its largest and deadliest outbreak of the Dengue virus (DENV), reporting the highest-ever recorded annual cases and deaths. Historically, most of the cases were recorded in the capital city, Dhaka. We aimed to characterize the geographical transmission of DENV in Bangladesh.

Sustainable Synthesis of Nitrogen-Embedded CuS Quantum Dots for In Vitro and In Vivo Breast Cancer Management.

The burgeoning field of nanomedicine is exploring quantum dots for cancer theranostics. In recent years, chemically engineered copper sulfide (CuS) quantum dots (QDs) have emerged as a multifunctional platform for fluorescence-based sensors with prominent applications in imaging and chemodynamic therapy of tumor cells. The present study demonstrates the sustainable synthesis of nitrogen-embedded copper sulfide (N@CuS) quantum dots for the first time and unveils their potential application in in vitro and in vivo breast cancer management.

Ketorolac disturbs proteasome functions and induces mitochondrial abnormality-associated apoptosis.

Non-steroidal anti-inflammatory drugs (NSAIDs) are recommended to treat moderate-to-severe pain. Previous studies suggest that NSAIDs can suppress cellular proliferation and elevate apoptosis in different cancer cells. Ketorolac is an NSAID and can reduce the cancer cells' viability.

Inferring gene-pathway associations from consolidated transcriptome datasets: an interactive gene network explorer for .

Although an established model organism, remains comparatively inaccessible to high throughput screens, and alternative bioinformatic approaches still rely on unconnected datasets and outdated algorithms. Here, we report a new approach to consolidating RNA-seq and microarray data based on a systematic exploration of parameters and computational controls, enabling us to infer functional gene associations from their co-expression patterns. To illustrate the power of this approach, we took advantage of new data regarding a previously studied pathway, the biogenesis of a secretory organelle called the mucocyst.

Extract from endophytic isolates stimulates seed germination of the host and protocorm development of non-host orchids.

We isolated endophytic strains from the healthy roots, stems, and leaves of to investigate their plant growth-promoting activities in vitro. Subsequently, Indole acetic acid (IAA) was quantified and the gene (responsible for IAA synthesis in fungi) was amplified and sequenced. Finally, a germination assay was performed with seeds of and a plant growth assay with protocorms of to test their plant growth-promoting activities.

sp. nov., a novel cellulase-producing black yeast-like fungi isolated from ginger in India.

The genus , known for its melanized, yeast-like appearance, includes a diverse group of fungi with significant implications across various fields. An isolate representing a novel species was identified within this genus from a ginger tuber from India, based on morphological characteristics and molecular phylogenetic analysis. Phylogenetic analysis of the D1/D2 domain of the 26S LSU rRNA gene, SSU rRNA gene and the internal transcribed spacer (ITS) region confirmed this strain as a new species.

Leveraging Evolutionary Immunology in Interleukin-6 and Interleukin-17 Signaling for Lung Cancer Therapeutics.

Lung cancer is among the most common instances of cancer subtypes and is associated with high mortality rates. Due to the availability of fewer therapies and delayed clinical investigations, the number of cancer incidences is rising dramatically. This is possibly an effect of immune modulations and chemotherapeutic drugs that raises cancer resistance.

HuD regulates apoptosis in N2a cells by regulating Msi2 expression.

HuD plays a critical role in neurite outgrowth, neuronal plasticity, and survival. However, HuD autoantibodies from patients with paraneoplastic gut dysmotility can trigger the apoptotic cascade in human neuroblastoma cell line and myenteric neurons. The mechanism by which HuD regulates the apoptotic pathway is unclear.

Comparison of Taxonomic Resolutions of Various Typing Methods for and Species Isolated from Landfill Leachate.

Anaerobic bacteria and methanogenic archaea at municipal landfill dumping sites play a vital role in the landfill ecology, waste degradation, global warming and climate change. Although landfill works as a cheap way of solid waste management, unmanaged landfill plays a significant role in spreading pollutants and pathogens in natural ecosystems. The genera and are important groups of anaerobic microbes from a public and environmental health perspective.

Bacterial diversity along the geothermal gradients: insights from the high-altitude Himalayan hot spring habitats of Sikkim.

Geothermal habitats present a unique opportunity to study microbial adaptation to varying temperature conditions. In such environments, distinct temperature gradients foster diverse microbial communities, each adapted to its optimal niche. However, the complex dynamics of bacterial populations in across these gradients high-altitude hot springs remain largely unexplored.

Uncovering the Significance of JNK/AKT Axis in the Autophagic Regulation of Leishmania major Infection.

The role of autophagy in host induced by infection of parasites of the Leishmania genus remains inadequately understood. Leishmania parasites modulate host macrophages to promote its survival by inducing autophagy response in the host cell. In this study, we conducted an investigation of L.

Messages in CD40L are encrypted for residue-specific functions.

CD40-CD40-ligand (CD40L) interaction plays crucial immunoregulatory roles, as CD40 signals through different signaling intermediates to convert the messages from CD40L to effector functions. Being a TNFα receptor family member, CD40 binds TNFα receptor-associated factors, assembles signalosome complexes and decrypts the messages from CD40L through different signaling modules to result in residue-specific effector functions. The evidence for such a residue-specific message encryption first came from the CD40L mutations resulting in X-linked hyper-IgM syndrome, as the extent of effects varied with the residue mutated.

Apoptosis-Inducing Activity of a 2-Hydroxyphenyl Benzamide-Based Self-Assembled Anion Channel.

Despite the significant interest in designing artificial ion channels, there is limited availability of channel-forming molecules to tackle complex issues, especially in biological systems. Moreover, a major challenge is the scarcity of chloride transporters that can selectively induce toxicity in cancer cells while minimizing harm to normal healthy cells. This work reports a series of 2-hydroxyphenyl benzamide-based small molecules 1 a-1 c, which self-assemble to form barrel rosette-type artificial ion channels that adequately transport chloride ions across membranes.

Regulating translation in aging: from global to gene-specific mechanisms.

Aging is characterized by a decline in various biological functions that is associated with changes in gene expression programs. Recent transcriptome-wide integrative studies in diverse organisms and tissues have revealed a gradual uncoupling between RNA and protein levels with aging, which highlights the importance of post-transcriptional regulatory processes. Here, we provide an overview of multi-omics analyses that show the progressive uncorrelation of transcriptomes and proteomes during the course of healthy aging.

A novel ITGB8 transcript variant sustains ovarian cancer cell survival through genomic instability and altered ploidy on a mutant p53 background.

Background: Transcript variants and protein isoforms are central to unique tissue functions and maintenance of homeostasis, in addition to being associated with aberrant states such as cancer, where their crosstalk with the mutated tumor suppressor p53 may contribute to genomic instability and chromosomal rearrangements. We previously identified several novel splice variants in ovarian cancer RNA-sequencing datasets; herein, we aimed to elucidate the biological effects of the Integrin Subunit Beta 8 variant (termed pITGB8-205).

Methods: Resolution of the full-length sequence of pITGB8-205 through rapid amplification of cDNA ends (RACE-PCR).

Functional implications of the exon 9 splice insert in GluK1 kainate receptors.

Kainate receptors are key modulators of synaptic transmission and plasticity in the central nervous system. Different kainate receptor isoforms with distinct spatiotemporal expressions have been identified in the brain. The GluK1-1 splice variant receptors, which are abundant in the adult brain, have an extra fifteen amino acids inserted in the amino-terminal domain (ATD) of the receptor resulting from alternative splicing of exon 9.

Neuroimmune communication of the cholinergic system in gut inflammation and autoimmunity.

Neuroimmune communication in the body forms a bridge between two central regulatory systems of the body, i.e., nervous and immune systems.

The fly brain lands in Tokyo: A report on the 3rd Asia Pacific Drosophila Neurobiology Conference.

The third Asia Pacific Drosophila Neurobiology Conference (APDNC3) was held in the Wako Campus of RIKEN in Tokyo, Japan, from February 27th to March 1st, 2024. While APDNC2 was held in Taiwan in 2019, the global coronavirus pandemic enforced a long hiatus. Hence, APDNC3 was a much-anticipated meeting that attracted ~218 scientists from 18 different countries and regions, 154 from outside Japan.

The secretory pathway in Tetrahymena is organized for efficient constitutive secretion at ciliary pockets.

In ciliates, membrane cisternae called alveoli interpose between the plasma membrane and the cytoplasm, posing a barrier to endocytic and exocytic membrane trafficking. One exception to this barrier is plasma membrane invaginations called parasomal sacs, which are adjacent to ciliary basal bodies. By following a fluorescent secretory marker called ESCargo, we imaged secretory compartments and secretion in these cells.

The cGAS-STING mediated crosstalk between innate immunity and autophagy in leishmaniasis using mathematical modeling: Uncovering new therapeutic avenues.

The present paper deals with the investigation into the cGAS-STING pathway, focusing on the signaling of interferons through mathematical modeling and identifying a significant positive feedback loop regulated by STING for activation of type 1 interferons (IFN-1). Cyclic GMP-AMP synthase (cGAS) is responsible for detecting cytosolic DNA and initiating the STING (stimulator of interferon genes) pathway, which in turn causes the synthesis of pro-inflammatory cytokines and type I interferons. In addition to being crucial for pathogen identification, this route interacts with autophagy, a cellular mechanism that is necessary for immunological homeostasis and pathogen removal.

Proteomic Analysis of Microsomal Proteins Reveals That MVP Is Crucial for the Secretion of GDF-15, Which in Turn Promotes the Neuroendocrine Differentiation of PCa Cells.

Neuroendocrine prostate cancer (NEPC) is an aggressive androgen-independent PCa (AIPC) that tends to resist treatment. Understanding its progression and resistance could improve survival outcomes. Previous studies on PCa cells highlighted microsomal proteins' role in PCa progression, but their role in the progression of NEPC remains unclear.

Prolonged exposure to insulin might cause epigenetic alteration leading to insulin resistance.

Glucose homeostasis is maintained by insulin. Insulin resistance is caused by multiple factors including hereditary factors and diet. The molecular mechanism underlying insulin resistance (IR) is not completely understood.

Comparative Proteogenomic Approaches for Mapping the Global Proteome of the Unsequenced Leishmania Vector Phlebotomus papatasi.

The rapid improvements in next-generation sequencing technologies have made it possible to quickly access in-depth genome sequence data. This has resulted in a flurry of genome sequences of various organisms being published and made publicly available in the last two decades. However, not all organisms have genome sequence data available.