Aberrant expansion of CD177 neutrophils promotes endothelial dysfunction in systemic lupus erythematosus via neutrophil extracellular traps.

Objective: Aberrant neutrophil activation is implicated in the pathogenesis of systemic lupus erythematosus (SLE) and its related comorbidities. We found that CD177 was one of the most highly up-regulated genes at the transcriptional level in purified neutrophils from SLE patients. In this study, we aimed to explore the role of CD177 neutrophils in the pathogenesis of SLE.

Decrease in the internal quality control intermediate reproducibility imprecision of Cystatin C results in China in the years from 2014 to 2023.

Objectives: We evaluated the intermediate reproducibility imprecision of cystatin C results based on internal quality control (IQC) data.

Methods: Five performance specifications [1/3 total allowable error (TEa), 1/4TEa and three levels performance specifications based on biological variation] were used to calculate the proportion of laboratories with CVs less than or equal to the performance specifications, namely the pass rate. Based on the reference interval of Chinese adult serum cystatin C (0.

Flow-cytometric lymphocyte subsets enumeration: comparison of single/dual-platform method in clinical laboratory with dual-platform extended PanLeucogating method in reference laboratory.

Objectives: To improve the accuracy of lymphocyte subsets counts, a traceable dual-platform extended PanLeucogating method (DPP) of absolute cell counts of lymphocyte subsets was introduced, and consistency was evaluated by comparing conventional single/dual-platform method with DPP.

Methods: The DPP for absolute lymphocyte subsets counts was established by multiplying the percentage of lymphocyte subsets in total white blood cells (WBC) measured by flow cytometer with the total WBC counts. DPP-R was defined as the use of the total WBC counts measured in reference laboratory that was traceable to reference method recommended by International Council for Standardization in Hematology (ICSH).

TRIM32 positively regulates c-di-GMP-Induced type I interferon signaling pathway in Listeria monocytogenes infection.

Listeria monocytogenes (Lm) poses a significant threat to human health. TRIM32, an E3 ubiquitin ligase, plays a critical role in regulating immune responses to pathogen infections. Previous studies have shown that TRIM32 deficiency significantly impairs IFN-β production.

Optimizing HIV testing: Comparing diagnostic signatures and assay performance in ART-treated and general screening populations.

Introduction: Accurate and early HIV detection is crucial for improving outcomes and controlling transmission. This study compares HIV marker patterns and evaluates detection assay performance in general screening and ART-treated populations to enhance diagnostic accuracy and public health.

Methods: A total of 196 blood donor samples that were initially reactive in blood center screening were collected across 17 provinces in China, along with 126 ART samples from people living with HIV (PLWH).

Construction of RNA reference materials for improving the quantification of transcriptomic data.

RNA reference materials and their corresponding reference datasets act as the 'ground truth' for the normalization of experimental values and are indispensable tools for reliably measuring intrinsically small differences in RNA-sequencing data, such as those between molecular subtypes of diseases in clinical samples. However, the variability in 'absolute' expression profiles measured across different batches, methods or platforms limits the use of conventional RNA reference datasets. We recently proposed a ratio-based method for constructing reference datasets.

Analytical performance evaluation and optimization of serum 25(OH)D LC-MS/MS measurement.

Objectives: Measuring serum 25-hydroxyvitamin D is key in clinical labs, but inter-laboratory variations risk diagnostic errors. This study evaluates the performance of current in-house liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods used in top Chinese clinical laboratories and proposes an optimized method for improving serum 25(OH)D measurement accuracy and reliability.

Methods: Seven serum pools with different concentrations of 25(OH)D were prepared and sent to 12 participating laboratories for multiple repeat analysis with their current in-house LC-MS/MS methods and then an optimized LC-MS/MS method.

The ER Stress-related Gene Prognostic Signature for Predicting Chemosensitivity and Prognosis in AML.

Introduction: Acute myeloid leukemia is characterized by high heterogeneity, and the current European Leukemia Net (ELN) risk stratification system is not universally applicable to all AML patients, requiring approximately three weeks for testing.

Aim: This study aimed to develop an applicable prognostic tool capable of addressing the limitations of current methods. We selected AML patients from the clinic and TCGA database to explore the role of ER stress in response to chemotherapy.

COVID-19 Vaccine Preferences in China: A Comparison of Discrete Choice Experiment and Profile Case Best-Worst Scaling.

Objectives: Little is known about the diversity of residents' preferences for COVID-19 vaccines during the time when COVID-19 management was downgraded in China. This study aims to investigate these preferences using discrete choice experiment (DCE) and profile case best-worst scaling (BWS-2), and to assess the concordance between these two methods.

Methods: Chinese residents recruited for the online survey were asked to evaluate COVID-19 vaccine profiles through both DCE and BWS-2 from April to July 2023.

Indoxyl sulfate (IS) mediates pro-inflammatory responses in severe pneumonia in patients with rheumatoid arthritis associated interstitial lung disease.

Object: Patients with rheumatoid arthritis-associated interstitial lung disease (RA-ILD) have a high risk of serious infection, in particular severe pneumonia. This study aimed to investigate the transcriptional landscape, lower respiratory tract (LRT) microbiome and metabolomic profiles in the lung of RA-ILD patients with pneumonia.

Method: A total of 10 RA-ILD with pneumonia were enrolled in this study.

Unveiling the biological variation of lymphocyte subset counts: Insights from a full-spectrum flow cytometer and the BD Multitest 6-Color TBNK kit.

Background And Aims: To estimate the biological variation (BV) for lymphocyte subset counts in healthy adults based on full-spectrum flow cytometry (FS-FCM) and the most commonly used BD Multitest 6-Color TBNK kit in China.

Materials And Methods: The study was designed according to the BV Data Critical Appraisal Checklist (BIVAC). Peripheral blood samples were collected from 60 healthy adults every two weeks for a period of 20 weeks (10 samples from each subject).

Comparative analysis of allowable total error specifications for coagulation factor assays utilizing China National External quality assessment scheme data and biological variation data.

Background: It is necessary and challenging to establish reasonable and feasible total error specifications for coagulation factor assays for quality control and assessment. The aim of this study is to establish new total error specifications for coagulation factor assays by combining External Quality Assessment data with reliable biological variation data.

Methods: Data from China National External Quality Assessment Scheme (28,408 results from 1,381 laboratories) were analyzed, stratifying External Quality Assessment data by reference intervals to establish concentration-dependent total error specifications.

Associations of phenols, parabens, and phthalates with biological aging: stratified analyses by chronological age and lifestyle in NHANES 2005-2010.

Humans are widely exposed to phenols, parabens, and phthalates with health risks, while the effects of these chemicals on biological aging remain unclear. Among 3,441 adults in the National Health and Nutrition Examination Survey 2005-2010, phenol, paraben, and phthalate concentrations were measured and phenotypic age acceleration (PhenoAgeAccel) was calculated. Linear regression and weighted quantile sum (WQS) regression were used to evaluate the associations of single and mixed chemicals with PhenoAgeAccel.

Associations of the hs-CRP/HDL-C ratio with cardiovascular disease among US adults: Evidence from NHANES 2015-2018.

Background And Aims: Inflammation, lipid signaling, and their interplay are involved in the pathogenesis and development of cardiovascular diseases (CVDs), while the relationships of composite indices combining inflammation and lipids with CVD remained inexplicit.

Methods And Results: Our study enrolled 8581 adults from the National Health and Nutrition Examination Survey 2015-2018. Logistic regression model was applied to assess the associations of high-sensitivity C-reactive protein (hs-CRP)-to-high-density lipoprotein cholesterol (HDL-C) ratio with CVD prevalence.

Associations of aldehyde exposure with serum uric acid and hyperuricemia among U.S. adults from NHANES 2013-2014.

Background: Aldehyde is a kind of important environmental pollutant, which has been demonstrated to be associated with increased risks of various chronic diseases with the economic and social development. However, the effects of aldehydes on serum uric acid (SUA) and hyperuricemia remained inexplicit, and the potential mediating pathways for this relationship needed to be addressed.

Methods: This study investigated the associations of individual and mixed aldehydes with SUA and hyperuricemia among 1588 U S.

Interlaboratory variability of HER2 fluorescence in situ hybridization testing in breast cancer: results of a multicenter proficiency-testing ring study in China.

Background: Accurate detection of human epidermal growth factor receptor 2 (HER2) gene amplification via fluorescence in situ hybridization (FISH) is necessary to determine HER2 status. Although many attempts have been made to increase the consistency of the results, the actual situation still needs to be determined. To investigate the latest interlaboratory variability of HER2 FISH testing for breast cancer, a multicenter proficiency-testing ring study was conducted in China.

Advances in laboratory diagnosis of Sjogren's disease in children.

Sjogren's disease (SjD) in children is a rare chronic autoimmune disease not fully recognized due to clinical manifestations different from adults. As such, new objective indicators are needed to supplement existing markers and assist in diagnosis. This review summarizes pathogenesis of SjD in children, current diagnostic criteria and research progress in laboratory diagnosis including serologic testing, saliva and tear analysis, histopathological examination as well as emerging markers of interest.

[Analytical performance evaluation of Flash10 point-of-care testing in severe acute respiratory syndrome coronavirus 2 nucleic acid detection].

To evaluate the analytical performance of Flash10 point-of-care testing (POCT) in the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid. The analytical performance evaluation of the Flash10 POCT system and its matching kits was carried out based on the non-infectious phage virus-like particles (VLPs) samples and inactivated viral culture samples. The limit of detection (LoD) was evaluated by testing gradient-diluted non-variant and Omicron variants (BA.

Analysis of antibiotic susceptibility and genomic characteristics of Helicobacter pylori by whole-genome resequencing in Northern China.

Introduction: Helicobacter pylori (H. pylori) is a major human pathogen whose increased antibiotic resistance poses a serious threat to human health.

Aim: The aim of this study is to further explore the association between H.

Role of STING Deficiency in Amelioration of Mouse Models of Lupus and Atherosclerosis.

Objective: Systemic lupus erythematosus (SLE) is a systemic autoimmune syndrome characterized by autoreactive responses to nucleic acids, dysregulation of the type I interferon (IFN-I) pathway, and accelerated atherosclerosis. The stimulator of IFN genes (STING), a cytosolic DNA sensor, has pathogenic implications in various inflammatory diseases. However, its specific role in SLE pathogenesis, particularly in tissue damage, remains unclear.

Pilot Surveillance of , , and Among Chinese Blood Donors - Xinjiang, Inner Mongolia, and Heilongjiang PLADs, China, 2022-2023.

What Is Already Known About This Topic?: Recently, tick-borne pathogens transmitted through blood transfusions have posed new risks to blood safety.

What Is Added By This Report?: We developed a quality control system for nucleic acid testing (NAT) for , , and , evaluated five Triplex-NAT kits, and conducted external quality assessments of blood centers. This study screened 92,700 blood donors from Heilongjiang, Inner Mongolia, and Xinjiang provincial-level administrative divisions during 2022-2023.

Plasma concentrations of venetoclax and Pharmacogenetics correlated with drug efficacy in treatment naive leukemia patients: a retrospective study.

Venetoclax (VEN) was the only Bcl-2 inhibitor approved yet and showed large differences in clinical efficacy. The aim of the study was to explore the relationships between the plasma concentration and efficacy of VEN, and identify potential influencing factors. A retrospective cohort study was conducted and a total of 76 trough (C) and 91 6 h post-dose plasma concentration (C) blood concentrations of VEN were collected in 54 patients.

Interlaboratory consistency of promoter methylation detection in colorectal cancer using the post-optimized materials.

Fecal DNA-based Syndecan 2 () methylation detection is a promising non-invasive strategy for early colorectal cancer (CRC) screening. In China, commercial assays for methylation detection vary in sensitivity and specificity, yet there is no standardized external quality assessment (EQA) to ensure accuracy. This study utilized CRISPR-Cas9 and homology-directed repair (HDR) technologies to edit the promoter in 293T cells, creating hypermethylated and heterogeneous cell lines.