Molecular Detection and Isolation of Lumpy Skin Disease Virus during an Outbreak in West Hararghe Zone, Eastern Ethiopia.

Lumpy skin disease (LSD) is a highly contagious viral disease that causes significant economic losses in cattle populations globally. This study aimed to isolate and detect the LSD virus responsible for an outbreak in selected areas (Daaroo Labuu, Hawwii Guddina, and Gumbi Bordede district) of the West Hararghe Zone in Ethiopia between January 2020 and December 2021. Out of the 625 animals examined for the presence of LSD, only 73 animals showed clinical signs, and skin scrapes were collected from these animals for further analysis.

Prevalence of paratuberculosis in cattle based on gross and microscopic lesions in Ethiopia.

Background: Paratuberculosis, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic progressive granulomatous enteritis mainly affecting domestic and wild ruminants worldwide. Although paratuberculosis could be prevail in Ethiopia, there is a scarcity of epidemiological data on paratuberculosis in the country.

Seroprevalence and risk factors for Toxoplasma gondii infection among pregnant women at Debre Markos Referral Hospital, northwest Ethiopia.

Background: To assess the seroprevalence and risk factors of Toxoplasma gondii infection in pregnant women at the Debre Markos Referral Hospital, northwest Ethiopia.

Methods: A facility-based cross-sectional study was undertaken among pregnant women from March 2020 to May 2021. Sociodemographic and clinical data were collected from randomly selected participants.

Spoligotype analysis of isolates from cattle and assessment of zoonotic TB transmission among individuals working in bovine TB-infected dairy farms in Ethiopia.

Bovine tuberculosis (bTB) is a disease with impact on dairy productivity, as well as having the potential for zoonotic transmission. Understanding the genetic diversity of the disease agent is important for identifying its routes of transmission. Here we investigated the level of genetic diversity of isolates and assessed the zoonotic potential in risk groups of people working in bTB-infected dairy farms in central Ethiopia.

Isolation and Determination of Antibacterial Sensitivity Characteristics of from Lactating Cows in West Shewa Zone, Ethiopia.

is one of the etiologies of bovine mastitis, hindering milk production and productivity in dairy farms. This study was aimed at assessing the distribution of bovine mastitis and the isolation rate of in milked cows of West Shewa Zone. The clinical mastitis was diagnosed by physical methods including observation and palpation, whereas the subclinical mastitis was tested by the California mastitis test (CMT).

Seroprevalence of Bluetongue Virus Antibodies in Ovine in Maji District of West Omo Zone, Southwest Ethiopia.

Background: Bluetongue (BT) disease is an arthropod-transmitted viral disease of domestic and wild ruminant species caused by Bluetongue virus (BTV). It is of most importance in sheep and endemic primarily in the tropical and subtropical regions where vectors ( species) are present.

Materials And Methods: A cross-sectional study was conducted in July-November 2019 to examine the seroprevalence of BTV infection in ovine in Maji district of West Omo zone.

Seroprevalence and associated risk factors for foot and mouth disease virus seropositivity in cattle in selected districts of Gamo zone, Southern Ethiopia.

Background: Foot and mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals, which hampers livestock production and productivity in Ethiopia. This cross-sectional study was conducted from January to December 2021 to estimate the seroprevalence of FMD in cattle and to assess farmers' knowledge about the disease in selected districts of the Gamo zone. Three districts and two kebeles (smallest administrative division) from each district were purposively sampled using a simple random sampling technique to select individual animals from each kebeles.

High seroprevalence of Leishmania infantum infection in dogs and its associated risk factors in selected towns of Southwest and West Shewa zones of Oromia, Ethiopia.

Background: Leishmania infantum infection can result in serious vector-borne zoonotic disease such as visceral leishmaniasis. The objectives of the study were to estimate the seroprevalence and associated risk factors in Weliso, Ambo, and Ejaji towns in the Southwest and West Shewa zones, Oromia, Ethiopia.

Methods: A cross-sectional study was conducted from October 2019 to September 2020.

Temporal and Spatial Patterns and a Space-Time Cluster Analysis of Foot-and-Mouth Disease Outbreaks in Ethiopia from 2010 to 2019.

Foot-and-mouth disease (FMD) is an endemic disease in Ethiopia, although space-time cluster and monthly variation studies have never been assessed at national level. The current study aimed to identify the spatial and temporal distribution of FMD outbreaks in Ethiopia from national outbreak reports over a period of ten years from 1 January 2010 to 31 December 2019. To this end, a total of 376,762 cases and 1302 outbreaks from 704 districts were obtained from the Minister of Agriculture for analyses.

Community Wastewater-Based Surveillance Can Be a Cost-Effective Approach to Track COVID-19 Outbreak in Low-Resource Settings: Feasibility Assessment for Ethiopia Context.

Wastewater surveillance systems have become an important component of COVID-19 outbreak monitoring in high-income settings. However, its use in most low-income settings has not been well-studied. This study assessed the feasibility and utility of wastewater surveillance system to monitor SARS-CoV-2 RNA in Addis Ababa, Ethiopia.

Isolation and Molecular Characterization of from Outbreaks in Southern Ethiopia, 2020.

Peste des petits ruminants (PPR) is one of the most important transboundary diseases of small ruminants. In this study, nasal and oral swabs ( = 24) were collected from sheep ( = 7) and goats ( = 17) with clinical signs in southern Ethiopia in March 2020. PPR virus was isolated on Vero dog cells expressing the signaling lymphocyte activation molecule (VDS) and screened using RT-qPCR.

Isolation and Molecular Detection of Newcastle Disease Virus from Field Outbreaks in Chickens in Central Ethiopia.

Background: Newcastle disease is a major viral disease of poultry. The virus is a major problem for chickens in Ethiopia and there is a scarcity of updated information on the virological and molecular status of confirmation of Newcastle disease outbreak cases in the country.

Methods: Newcastle disease outbreaks were investigated from February 2021 to October 2021 in central Ethiopia to isolate and detect the virus by cell culture and reverse transcriptase PCR.

Economic impact of a peste des petits ruminants outbreak and vaccination cost in northwest Ethiopia.

Peste des petits ruminants (PPR) is an important endemic disease of small ruminants in Ethiopia. While vaccination is widely used in the country to control the disease, quantitative estimates of the actual economic losses due to outbreaks and costs of vaccination are scarce. This study assessed the economic impact and costs of PPR vaccination in Metema district, northwest Ethiopia.

Utility of the Intradermal Skin Test in a Test-and-Cull Approach to Control Bovine Tuberculosis: A Pilot Study in Ethiopia.

Bovine tuberculosis (bTB) is one of the top three, high-priority, livestock diseases in Ethiopia and hence, the need for evaluation of potential control strategies is critical. Here, we applied the test-and-segregate followed by cull strategy for the control of bTB in the intensive Alage dairy farm in Ethiopia. All cattle reared on this farm were repeatedly skin tested using the Comparative Cervical Tuberculin (CCT) test for a total of five times between 2015 and 2021.

Seroepidemiology of Infectious Bursal Disease in Poultry Reared Under Backyard Production System in Wolaita Zone, Southern Ethiopia.

Background: Infectious bursal disease (IBD) has been known to cause high morbidity and mortality in chickens resulting in considerable financial losses to poultry producers. This study was performed with the objectives of estimating the seroprevalence and associated risk factors of IBD in backyard chickens in Wolaita zone, southern Ethiopia.

Methods: A total of 482 serum samples were collected from chickens reared under backyard systems using a multi-stage cross-sectional study design.

Porcine circovirus-2 in Africa: Identification of continent-specific clusters and evidence of independent viral introductions from Europe, North America and Asia.

Porcine circovirus-2 (PCV-2) is associated with several disease syndromes in domestic pigs that have a significant impact on global pig production and health. Currently, little is known about the status of PCV-2 in Africa. In this study, a total of 408 archived DNA samples collected from pigs in Burkina Faso, Cameroon, Cape Verde, Ethiopia, the Democratic Republic of the Congo, Mozambique, Nigeria, Senegal, Tanzania and Zambia between 2000 and 2018 were screened by PCR for the presence of PCV-2.

Isolation, Identification, and Antimicrobial Susceptibility Profiles of from Clinical Mastitis in Sebeta Town Dairy Farms.

A cross-sectional study was carried out in and around Sebeta town dairy farms, Finfinne special zone, Ethiopia, from December 2019 to May 2020 to isolate, identify, and test antimicrobial susceptibility profile of from clinical mastitis. A total of 116 milk samples were purposively collected from 57 lactating cows with clinical mastitis. Isolation and identification of were carried out by using primary and secondary biochemical tests.

Assessment of Biosecurity Status in Commercial Chicken Farms Found in Bishoftu Town, Oromia Regional State, Ethiopia.

A survey was undertaken from December 2017 to April 2018 to assess the biosecurity status of 44 commercial chicken farms established in Bishoftu town, Ethiopia, by interviewing farm owners using a structured questionnaire. The obtained data were summarized using frequency tables and analyzed with Pearson's chi-square test and Fischer's exact value using Stata 14 statistical software. From the assessed chicken farms, 31 (70.

Effect of Bovine Tuberculosis on Selected Productivity Parameters and Trading in Dairy Cattle Kept Under Intensive Husbandry in Central Ethiopia.

Bovine tuberculosis (BTB) has substantial impact on fertility, milk, and meat productivity in cattle. However, these assumptions are based on outdated data. Recent global studies on the impact of BTB on cattle productivity are scarce and show sometimes inconclusive and/or contradicting results.

First molecular detection of hepatitis E virus genome in camel and pig faecal samples in Ethiopia.

Background: Hepatitis E is an enteric and zoonotic disease caused by hepatitis E virus (HEV) that is mainly transmitted via the faecal-oral route through contaminated food or the environment. The virus is an emerging infectious agent causing acute human infection worldwide. A high seroprevalence of the disease was reported in pregnant women in Addis Ababa, Ethiopia, raising significant public health concern.

Seroprevalence and Molecular Detection of Foot and Mouth Disease Virus in Dairy Cattle Around Addis Ababa, Central Ethiopia.

Background: Foot and mouth disease is a debilitating and highly contagious transboundary disease of cattle that can cause a huge economical loss globally. It is a notifiable disease in Ethiopia, and it is thought to be causing a decrease in cattle productivity and production.

Methods: A cross-sectional study and outbreak investigation were performed to estimate seroprevalence, identify associated factors and serotypes of FMDV in dairy cattle around Addis Ababa.

Innate Immune Responses to Wildtype and Attenuated Sheeppox Virus Mediated Through RIG-1 Sensing in PBMC .

Sheeppox (SPP) is a highly contagious disease of small ruminants caused by sheeppox virus (SPPV) and predominantly occurs in Asia and Africa with significant economic losses. SPPV is genetically and immunologically closely related to goatpox virus (GTPV) and lumpy skin disease virus (LSDV), which infect goats and cattle respectively. SPPV live attenuated vaccines (LAVs) are used for vaccination against SPP and goatpox (GTP).