Genome-Wide Identification and Expression Profile of () Gene Family in L.

The biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), which are essential for sesquiterpenes and triterpenes, respectively, is primarily governed by the mevalonate pathway, wherein () plays a pivotal role. This study identified eight members of the FPS gene family in , designated -, through bioinformatics analysis, revealing their distribution across several chromosomes and a notable tandem gene cluster. The genes exhibited strong hydrophilic properties and key functional motifs crucial for enzyme activity.

The Potassium Utilization Gene Network in and Functional Validation of Gene in Response to Potassium Deficiency.

Potassium, an essential inorganic cation, is crucial for the growth of oil crops like L. Given the scarcity of potassium in soil, enhancing rapeseed's potassium utilization efficiency is of significant importance. This study identified 376 potassium utilization genes in the genome of ZS11 through homologous retrieval, encompassing 7 functional and 12 regulatory gene families.

Advances in Protein Kinase Regulation of Stress Responses in Fruits and Vegetables.

Fruits and vegetables (F&Vs) are essential in daily life and industrial production. These perishable produces are vulnerable to various biotic and abiotic stresses during their growth, postharvest storage, and handling. As the fruit detaches from the plant, these stresses become more intense.

Cytosine Methylation Changes the Preferred Cis-Regulatory Configuration of Arabidopsis WUSCHEL-Related Homeobox 14.

The Arabidopsis transcription factor WUSCHEL-related homeobox 14 (AtWOX14) plays versatile roles in plant growth and development. However, its biochemical specificity of DNA binding, its genome-wide regulatory targets, and how these are affected by DNA methylation remain uncharacterized. To clarify the biochemistry underlying the regulatory function of AtWOX14, using the recently developed 5mC-incorporation strategy, this study performed SELEX and DAP-seq for AtWOX14 both in the presence and absence of cytosine methylation, systematically curated 65 motif models and identified 51,039 genomic binding sites for AtWOX14, and examined how 5mC affects DNA binding of AtWOX14 through bioinformatic analyses.

Molecular Docking Studies and In Vitro Activity of Pancreatic Lipase Inhibitors from Yak Milk Cheese.

Pancreatic lipase serves as a primary trigger for hyperlipidemia and is also a crucial target in the inhibition of hypercholesterolemia. By synthesizing anti-hypercholesterolemic drugs such as atorvastatin, which are used to treat hypercholesterolemia, there were some side effects associated with the long-term use of statins. Based on this idea, in the present study, we identified peptides that inhibited PL by virtual screening and in vitro activity assays.

Identifying a Biocontrol Bacterium with Disease-Prevention Potential and Employing It as a Powerful Biocontrol Agent Against .

Biocontrol microbes are environment friendly and safe for humans and animals. To seek biocontrol microbes effective in suppressing is important for tomato production. is a soil-borne pathogen capable of causing wilt in numerous plant species.

Integrated Genetic Diversity and Multi-Omics Analysis of Colour Formation in Safflower.

Safflower ( L.) is a medicinal and edible cash crop that is widely cultivated worldwide. However, the genetic diversity of safflower germplasm resources and the reasons for the variations in safflower flower colour remain unclear.

Re-Examination Characterization and Screening of Stripe Rust Resistance Gene of Wheat Gene Family Based on the Transcriptome in Xinchun 32.

Pathogenesis-related protein-1 (PR1) encodes a water-soluble protein produced in plants after pathogen infection or abiotic stimulation. It plays a crucial role in plant-induced resistance by attacking pathogens, degrading cell wall macromolecules and pathogen toxins, and inhibiting the binding of viral coat proteins to plant receptor molecules. Compared to model plants, the mechanism of action of PR1 in wheat remains underexplored.

Network Pharmacology Approach and Experimental Verification to Explore the Anti-NSCLC Mechanism of Grifolic Acid.

Lung cancer is the leading cause of cancer-related death. Non-small cell lung cancer (NSCLC) accounts for 85% of all lung cancers and over 60% express wild-type EGFR (WT-EGFR); however, EGFR tyrosine kinase inhibitors (TKIs) have limited effect in most patients with WT-EGFR tumors. In this study, we applied network pharmacology screening and MTT screening of bioactive compounds to obtain one novel grifolic acid that may inhibit NSCLC through the EGFR-ERK1/2 pathway.

Exogenous SNP Alleviates Drought Stress in Wheat During the Grain-Filling Stage by Modulating Gene Transcription.

Drought stress severely damages wheat growth and photosynthesis, and plants at the grain-filling stage are the most sensitive to drought throughout the entire period of development. Exogenous spraying of sodium nitroprusside (SNP) can alleviate the damage to wheat caused by drought stress, but the mechanism regulating the proline pathway remains unknown. Two wheat cultivars, drought-sensitive Zhoumai 18 and drought-tolerant Zhengmai 1860, were used as materials when the plants were cultivated to the grain-filling stage.

Enhancing Soybean Salt Tolerance with GSNO and Silicon: A Comprehensive Physiological, Biochemical, and Genetic Study.

Soil salinity is a major global challenge affecting agricultural productivity and food security. This study explores innovative strategies to improve salt tolerance in soybean (), a crucial crop in the global food supply. This study investigates the synergistic effects of S-nitroso glutathione (GSNO) and silicon on enhancing salt tolerance in soybean ().

Localization and Molecular Cloning of the Gene for Melatonin Synthesis in Pigs.

Melatonin is synthesized in multiple tissues and organs of pigs, and existing studies have shown the presence of the melatonin-synthesizing enzyme ASMT protein. However, the genomic information for the gene has been lacking. The aim of this study was to locate the genomic information of the gene in pigs using comparative genomics analysis and then obtain the coding region information through molecular cloning.

Transcriptomics and Plant Hormone Analysis Reveal the Mechanism of Branching Angle Formation in Tea Plants ().

The branching angle of tea plants is a key factor in determining their branching structure, which significantly affects yield, suitability for mechanical harvesting, and overall plant architecture. However, the mechanisms underlying branching angle formation in tea plants remain unclear. In this study, we explored the mechanism of branching angle formation in tea plants by analysing the transcriptome and plant hormone levels of tea plant cultivars with different branching angles.

Loss of Affects m6A Modification but Not Semen Characteristics in Bull Spermatozoa.

N6-methyladenosine (m6A) modification is a key methylation modification involved in reproductive processes. gene editing (MT) in cattle is known to enhance muscle mass and productivity. However, the changes in m6A modification in MT bull sperm remain poorly understood.

Exogenous 24-Epibrassinolide Improves Low-Temperature Tolerance of Maize Seedlings by Influencing Sugar Signaling and Metabolism.

Low-temperature (LT) stress seriously affects the distribution, seedling survival, and grain yield of maize. At the seedling emergence stage, maize's coleoptile is one of the most sensitive organs in sensing LT signaling and, in general, it can envelop young leaves to protect them from LT damage. In addition, brassinolides (BRs) have been shown to enhance LT tolerance from various species, but the effects of BRs on coleoptiles in maize seedlings under LT stress are unclear.

Genome-Wide Identification of GmPIF Family and Regulatory Pathway Analysis of GmPIF3g in Different Temperature Environments.

Phytochrome-interacting factors (PIFs) play a crucial role in regulating plant growth and development. However, studies on soybean PIFs are limited. Here, we identified 22 GmPIF genes from the soybean genome and classified the GmPIF proteins into 13 subfamilies based on amino acid sequence homology, secondary and tertiary structures, protein structure, and conserved motifs.

The MADS-Box Transcription Factor EjAGL18 Negatively Regulates Malic Acid Content in Loquat by Repressing .

Malic acid is the major organic acid in loquat fruit, contributing to the sourness of fruit and affecting fruit flavor. However, the transcriptional regulation of malic acid in loquat is not well understood. Here, we discovered a MADS-box transcription factor (TF), EjAGL18, that regulated malic acid accumulation in loquat.

Integration of ATAC-Seq and RNA-Seq Reveals the Role of in Red Light-Induced Fruit Ripening in Strawberry.

Light is an important environmental factor affecting the ripening and quality of strawberry fruit. Previous studies have shown that red light treatment can promote strawberry ripening. Gene expression is closely associated with chromatin openness, and changes in chromatin accessibility are crucial for the binding of transcription factors to downstream regulatory sequences.

Hybridization Design and High-Throughput Screening of Peptides with Immunomodulatory and Antioxidant Activities.

With the increasing recognition of the role of immunomodulation and oxidative stress in various diseases, designing peptides with both immunomodulatory and antioxidant activities has emerged as a promising therapeutic strategy. In this study, a hybridization design was applied as a powerful method to obtain multifunctional peptides. A total of 40 peptides with potential immunomodulatory and antioxidant activities were designed and screened.

Prevalent and Drug-Resistant Phenotypes and Genotypes of Isolated from Healthy Cow's Milk of Large-Scale Dairy Farms in China.

is a common cause of mastitis in dairy cows, which results in large economic losses to the livestock industry. The aim of this study was to investigate the prevalence of in raw milk in China, assess antimicrobial drug susceptibility, and identify key antibiotic resistance genes carried by the isolates. In total, 350 raw milk samples were collected from large-scale farms in 16 provinces and cities in six regions of China to assess the resistance of .

Optimization of Compost and Peat Mixture Ratios for Production of Pepper Seedlings.

Substituting peat moss with compost derived from organic waste in plant nurseries presents a promising solution for reducing environmental impact, improving waste management, and enhancing soil health while promoting sustainable agricultural practices. However, selecting the appropriate proportions of both materials is crucial for each plant species. This study investigates the effects of different ratios of compost and peat mixtures on the growth and development of pepper seedlings.

The Isolation of Lead-Tolerant PGPR from Red Clover Soil and Its Role in Promoting the Growth of Alfalfa.

Alfalfa ( L.) is an outstanding species used for the remediation of heavy metal-contaminated soil, and our previous research has shown that PGPR can promote plant growth under high-concentration lead stress. This discovery has forced scientists to search for PGPR strains compatible with alfalfa to develop an innovative bioremediation strategy for the remediation of lead-contaminated soil.

Association Analysis of the Genomic and Functional Characteristics of Halotolerant J2-5-19 from the Rhizosphere of .

Halotolerant plant growth-promoting bacteria (HT-PGPB) have attracted considerable attention for their significant potential in mitigating salt stress in crops. However, the current exploration and development of HT-PGPB remain insufficient to meet the increasing demands of agriculture. In this study, an HT-PGPB isolated from coastal saline-alkali soil in the Yellow River Delta was identified as J2-5-19.

Four Decades of Biofertilizers: Advances and Future Prospects in Agriculture.

Over the past four decades, biofertilizers, which are microbial formulations based on species, have significantly contributed to sustainable agriculture by enhancing crop growth, improving soil health, and reducing the dependency on chemical fertilizers. species, particularly known for their ability to promote plant growth, fix nitrogen, solubilize phosphorus, and produce growth-promoting substances such as phytohormones and antibiotics, have emerged as key players in the development of eco-friendly agricultural solutions. This research utilizes bibliometric analysis based on 3,242 documents sourced from the Web of Science database to map the development, key contributions, and innovation within the field from 1985 to 2023.

Virulence of Bacteria Causing Mastitis in Dairy Cows: A Literature Review.

Bovine mastitis, a prevalent disease in dairy farms, exerts a profound negative influence on both the health and productivity of dairy cattle, leading to substantial economic losses for the dairy industry. The disease is associated with different bacterial agents, primarily Gram-positive cocci (e.g.