DNA-Assisted Assays for Studying Lipid Transfer Between Membranes.

Extended-synaptotagmins (E-Syts) are proteins located on the endoplasmic reticulum (ER) that tether the ER to the plasma membrane (PM) and regulate their lipid homeostasis via its lipid transfer module, the synaptotagmin-like mitochondrial lipid-binding protein (SMP) domain. Here, we describe in vitro DNA nanostructure-assisted lipid transfer assays investigating how the SMP domain transports lipids between membranes and associates with the membranes to extract and release lipids. The lipid transfer signal was detected through fluorescence resonance energy transfer (FRET).

Enantioselective Carbonylative Cyclization of Alkenes with C-H Bonds for Synthesis of γ-Lactams Bearing an α-Quaternary Carbon.

The development of effective synthetic methods to construct γ-lactams bearing a chiral α-quaternary carbon from relatively inert C(O)-H bonds with alkenes has been an elusive challenge. Herein, we used a naphthylamine-derived phosphine oxide ligating Ni and Al bimetallic catalyst to realize a carbonylative cyclization of formyl C-H bonds with alkenes, highly regio- and enantioselectively constructing γ-lactams bearing a chiral α-quaternary carbon in up to 99% yield and 98% ee. These γ-lactams proved to be versatile synthetic precursors for many biologically active molecules.

Comprehensive analysis of PDE2A: a novel biomarker for prognostic value and immunotherapeutic potential in human cancers.

Phosphodiesterase 2A (PDE2A) plays a pivotal role in modulating cyclic nucleotide metabolism. Recent studies have shown that PDE2A is associated with some tumors, but its expression profiles, prognostic significance, and immunological roles in diverse cancer types remain unclear. Utilizing advanced bioinformatics tools, we performed a comprehensive analysis of PDE2A gene expression in multiple human cancers.

Long-term prognostic factors and outcomes in mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes: a clinical and biochemical marker analysis.

Background: MELAS (Mitochondrial Encephalomyopathy, Lactic Acidosis, and Stroke-like episodes) is a common subtype of mitochondrial encephalomyopathy. However, few studies have explored the relationship between biochemical markers and prognosis. This study aimed to explore the relationship between clinical and biochemical markers and prognosis of patients with MELAS.

DNA-mediated self-assembly oxidative damage amplifier combined with copper and MTH1 inhibitor for cancer therapy.

Chemo-dynamic therapy (CDT) has a great potential in tumor extirpation. It entails producing hypertoxic reactive oxygen species (ROS) that damage the DNA of tumor cells and other biomacromolecules. However, the efficiency of CDT is severely hampered by the massive presence of glutathione (GSH) in tumor cells and the interference of ROS defense systems, such as Mutt homolog 1 (MTH1) protein sanitizes ROS-oxidized nucleotide pools.

A Review on MXene/Nanocellulose Composites: Toward Wearable Multifunctional Electromagnetic Interference Shielding Application.

With the rapid development of mobile communication technology and wearable electronic devices, the electromagnetic radiation generated by high-frequency information exchange inevitably threatens human health, so high-performance wearable electromagnetic interference (EMI) shielding materials are urgently needed. The 2D nanomaterial MXene exhibits superior EMI shielding performance owing to its high conductivity, however, its mechanical properties are limited due to the high porosity between MXene nanosheets. In recent years, it has been reported that by introducing natural nanocellulose as an organic framework, the EMI shielding and mechanical properties of MXene/nanocellulose composites can be synergically improved, which are expected to be widely used in wearable multifunctional shielding devices.

Quinoidal Semiconductor Nanoparticles for NIR-II Photoacoustic Imaging and Photoimmunotherapy of Cancer.

Photoagents with ultra-high near-infrared II (NIR-II) light energy conversion efficiency hold great promise in tumor phototherapy due to their ability to penetrate deeper tissues and minimize damage to surrounding healthy cells. However, the development of NIR-II photoagents remain challenging. In this study, an all-fused-ring quinoidal acceptor-donor-acceptor (A-D-A) molecule, SKCN, with a BTP core is synthesized, and nanoparticles named FA-SNPs are prepared.

Discovery and Characterization of Chitin Synthase Inhibitors with Novel Mechanism for Control of .

, a highly destructive global pest, infests a wide range of plant species, including crucial food crops and ornamental plants. Effective control methods for this pest remain limited. Chitin synthase (CHS) is a key enzyme in the biosynthesis of chitin, which is essential for the growth and development of arthropods.

Investigating the causal relationship between immune factors and ankylosing spondylitis: insights from a Mendelian Randomization study.

Background: Despite previous studies indicating a close relationship between immune system and ankylosing spondylitis (AS), the causal relationship between them remains unclear.

Methods: Genome-wide association data were utilized to explore the causal link between 731 immune cells and AS using a bidirectional two-sample MR approach. The data included immune cell data from Orrù et al.

Clinical scoring systems, molecular subtypes and baseline [F]FDG PET/CT image analysis for prognosis of diffuse large B-cell lymphoma.

Diffuse large B-cell lymphoma (DLBCL) is a highly heterogeneous hematological malignancy resulting in a range of outcomes, and the early prediction of these outcomes has important implications for patient management. Clinical scoring systems provide the most commonly used prognostic evaluation criteria, and the value of genetic testing has also been confirmed by in-depth research on molecular typing. [F]-fluorodeoxyglucose positron emission tomography / computed tomography ([F]FDG PET/CT) is an invaluable tool for predicting DLBCL progression.

The additive interaction of healthy lifestyles and genetic susceptibility on colorectal cancer risk in prediabetes: a large population-based prospective cohort study.

Objective: We aimed to investigate the interrelationships among polygenic risk scores (PRS), healthy lifestyle factors (HLFs), and colorectal cancer (CRC) risk in individuals with prediabetes. To investigate whether adherence to HLFs influence CRC risk in those with elevated PRS within this specific population.

Methods: Data from 22,408 prediabetes participants without CRC at baseline were analyzed from the UK Biobank.

Implications of prenatal exposure to hyperandrogen for hippocampal neurodevelopment and autism-like behavior in offspring.

Autism spectrum disorder (ASD) is a highly heterogeneous neurodevelopmental disorder that significantly jeopardizes the physical and mental well-being of children. Autism spectrum disorder results from a combination of environmental and genetic factors. Hyperandrogenic exposure during pregnancy increases their risk of developing autism.

Disulfiram/Cu targeting FOXO6 modulates sensitivity of hepatocellular carcinoma to lenvatinib via disrupt choline metabolic.

Disulfiram/Cu(DSF/Cu) has a known pharmacokinetic and safety profile, exerting a strong antitumor effect. Oral tyrosine kinase inhibitors including lenvatinib are approved as first-line therapy for treating advanced unresectable hepatocellular carcinoma (HCC). These patients still have limited survival due to drug resistance.

Baicalein disrupts the KEAP1-NRF2 interaction to alleviate oxidative stress injury by inhibiting M1 macrophage polarization.

Macrophages are key players in maintaining the balance of tissues and dealing with inflammation, carrying out vital functions specific to different tissues while safeguarding the body against infections. The intricate interplay between oxidative stress and macrophage polarization and how this contributes to pneumonia is not fully understood. Herein, a predominant accumulation of baicalein in lung macrophages of pathogen-infected mice was observed by an alkynyl-modified probe.

Rapid Deployment of Antiviral Drugs Using Single-Virus Tracking and Machine Learning.

The outbreak of emerging acute viral diseases urgently requires the acceleration of specialized antiviral drug development, thus widely adopting phenotypic screening as a strategy for drug repurposing in antiviral research. However, traditional phenotypic screening methods typically require several days of experimental cycles and lack visual confirmation of a drug's ability to inhibit viral infection. Here, we report a robust method that utilizes quantum-dot-based single-virus tracking and machine learning to generate unique single-virus infection fingerprint data from viral trajectories and detect the dynamic changes in viral movement following drug administration.

Efficacy and safety of switching to insulin glargine 300 U/mL in people with type 2 diabetes uncontrolled on basal insulin in China: A post hoc subpopulation analysis of the INITIATION study.

Aims: To evaluate the efficacy and safety of insulin glargine 300 U/mL (Gla-300) in people with uncontrolled type 2 diabetes (T2D) switching from another basal insulin (BI).

Materials And Methods: INITIATION was an interventional, single-arm, phase IV study conducted in China. In this post hoc subpopulation analysis, the efficacy and safety of switching to Gla-300 was investigated in individuals with uncontrolled T2D (HbA1c 7.

F-PCS measurements on proteins in live mammalian cells.

Pseudocontact shift (PCS) contains rich structural information of proteins in structural and chemical biology. F-PCS is determined in live mammalian cells dual labelling of the target protein with a paramagnetic tag and a F-tag, which is achieved by varied reactivity of solvent exposed cysteines in selection of different types of tags. About 0.

Evaluation of the peripheral visual performance of DIMS spectacle lenses versus single vision lenses.

Purpose: This study evaluates differences in the visual field performance when wearing the Defocus Incorporated Multiple Segments (DIMS) spectacle lens compared to wearing a conventional single vision (SV) spectacle lens.

Methods: Twenty-one children aged 9-14 years with spherical equivalent refraction (SER) between -1.13D to -4.

Genomic island-encoded LmiA regulates acid resistance and biofilm formation in enterohemorrhagic O157:H7.

Enterohemorrhagic (EHEC) O157:H7 is an important intestinal pathogen that causes severe foodborne diseases. We previously demonstrated that the genomic island-encoded regulator LmiA activates the locus of enterocyte effacement (LEE) genes to promote EHEC O157:H7 adherence and colonization in the host intestine. However, whether LmiA is involved in the regulation of any other biological processes in EHEC O157:H7 remains largely unexplored.

Unmixing Autoencoder for Image Reconstruction from Hyperspectral Data.

Due to the complexity of samples and the limitations in spatial resolution, the spectra in hyperspectral imaging (HSI) are generally contributed to by multiple components, making univariate analysis ineffective. Although feature extraction methods have been applied, the chemical meaning of the compressed variables is difficult to interpret, limiting their further applications. An unmixing autoencoder (UAE) was developed in this work for the separation of the mixed spectra in HSI.

Gain of pancreatic beta cell-specific SCD1 improves glucose homeostasis by maintaining functional beta cell mass under metabolic stress.

Aims/hypothesis: The key pancreatic beta cell transcription factor v-maf musculoaponeurotic fibrosarcoma oncogene homologue A (MafA) is critical for the maintenance of mature beta cell function and phenotype. The expression levels and/or activities of MafA are reduced when beta cells are chronically exposed to diabetogenic stress, such as hyperglycaemia (i.e.

Catalytic Asymmetric Oxidative Coupling between C(sp)-H Bonds and Carboxylic Acids.

The direct enantioselective functionalization of C(sp)-H bonds in organic molecules could fundamentally transform the synthesis of chiral molecules. In particular, the enantioselective oxidation of these bonds would dramatically change the production methods of chiral alcohols and esters, which are prevalent in natural products, pharmaceuticals, and fine chemicals. Remarkable advances have been made in the enantioselective construction of carbon-carbon and carbon-nitrogen bonds through the C(sp)-H bond functionalization.

Reductive Dissolution Mechanisms of Manganese Oxide Mediated by Algal Extracellular Organic Matter and the Effects on 17α-Ethinylestradiol Removal.

Reductive dissolution of manganese oxide (MnO) is a major process that improves the availability of manganese in natural aquatic environments. The extracellular organic matter (EOM) secreted by algae omnipresent in eutrophic waters may affect MnO dissolution thus the fate of organic micropollutants. This study investigates the mechanisms of MnO reductive dissolution mediated by EOM and examines the effects of this process on 17α-ethinylestradiol degradation.

Three-dimensionally structured MoS@biochar breaks through the bottleneck in antibiotic wastewater treatment: Greater efficiency and self-motivated oxidation pathway.

Two-dimensional (2D) MoS has been widely used to remove antibiotics. However, low selectivity for antibiotic pollutants, dependence on applied energy and oxidant, and secondary contamination are still the bottlenecks of this system for treating antibiotic wastewater. In this study, we proposed a three-dimensional (3D) material (3MoS/BMBC@MF) based on MoS and biochar with melamine sponge as the backbone.