Improved ClickTags enable live-cell barcoding for highly multiplexed single cell sequencing.

Click chemistry-enabled DNA barcoding of cells provides a universal strategy for sample multiplexing in single-cell RNA-seq (scRNA-seq). However, current ClickTags are limited to fixed samples as they only label cells efficiently in methanol. Herein, we report the development of a new protocol for barcoding live cells with improved ClickTags.

Fungi stabilize multi-kingdom community in a high elevation timberline ecosystem.

Microbes dominate terrestrial ecosystems via their great species diversity and vital ecosystem functions, such as biogeochemical cycling and mycorrhizal symbiosis. Fungi and other organisms form diverse association networks. However, the roles of species belonging to different kingdoms in multi-kingdom community networks have remained largely elusive.

Real-time COVID-19 detection over chest x-ray images in edge computing.

Severe Coronavirus Disease 2019 (COVID-19) has been a global pandemic which provokes massive devastation to the society, economy, and culture since January 2020. The pandemic demonstrates the inefficiency of superannuated manual detection approaches and inspires novel approaches that detect COVID-19 by classifying chest x-ray (CXR) images with deep learning technology. Although a wide range of researches about bran-new COVID-19 detection methods that classify CXR images with centralized convolutional neural network (CNN) models have been proposed, the latency, privacy, and cost of information transmission between the data resources and the centralized data center will make the detection inefficient.

SiP Nanosheets: A Metal-Free Two-Dimensional Photocatalyst for Visible-Light Photocatalytic H Production and Nitrogen Fixation.

Two-dimensional (2D) semiconductors for photocatalysis are more advantageous than the other photocatalytic materials since the 2D semiconductors generally have large specific surface area and abundant active sites. Phosphorus silicon (SiP), with an indirect bandgap in bulk and a direct bandgap in the monolayer, has recently emerged as an attractive 2D material because of its anisotropic layered structure, tunable bandgap, and high charge carrier mobility. However, the utilization of SiP as a photocatalyst for photocatalysis has been scarcely studied experimentally.

Ensemble-Based Assimilation of Satellite All-Sky Microwave Radiances Improves Intensity and Rainfall Predictions for Hurricane Harvey (2017).

Ensemble-based data assimilation of radar observations across inner-core regions of tropical cyclones (TCs) in tandem with satellite all-sky infrared (IR) radiances across the TC domain improves TC track and intensity forecasts. This study further investigates potential enhancements in TC track, intensity, and rainfall forecasts via assimilation of all-sky microwave (MW) radiances using Hurricane Harvey (2017) as an example. Assimilating Global Precipitation Measurement constellation all-sky MW radiances in addition to GOES-16 all-sky IR radiances reduces the forecast errors in the TC track, rapid intensification (RI), and peak intensity compared to assimilating all-sky IR radiances alone, including a 24-hr increase in forecast lead-time for RI.

Anthropogenic Aerosols Modulated 20th-Century Sahel Rainfall Variability Via Their Impacts on North Atlantic Sea Surface Temperature.

The Sahel rainfall has a close teleconnection with North Atlantic sea surface temperature (NASST) variability, which has separately been shown to be affected by aerosols. Therefore, changes in regional aerosols emission could potentially drive multidecadal Sahel rainfall variability. Here we combine ensembles of state-of-the-art global climate models (the CESM and CanESM large ensemble simulations and CMIP6 models) with observational data sets to demonstrate that anthropogenic aerosols have significantly impacted 20th-century detrended Sahel rainfall multidecadal variability through modifying NASST.

Two transporters mobilize magnesium from vacuolar stores to enable plant acclimation to magnesium deficiency.

Magnesium (Mg) is an essential metal for chlorophyll biosynthesis and other metabolic processes in plant cells. Mg is largely stored in the vacuole of various cell types and remobilized to meet cytoplasmic demand. However, the transport proteins responsible for mobilizing vacuolar Mg2+ remain unknown.

An unexpected all-metal aromatic tetranuclear silver cluster in human copper chaperone Atox1.

Metal clusters, such as iron-sulfur clusters, play key roles in sustaining life and are intimately involved in the functions of metalloproteins. Herein we report the formation and crystal structure of a planar square tetranuclear silver cluster when silver ions were mixed with human copper chaperone Atox1. Quantum chemical studies reveal that two Ag 5s electrons in the tetranuclear silver cluster fully occupy the one bonding molecular orbital, with the assumption that this Ag cluster is Ag , leading to extensive electron delocalization over the planar square and significant stabilization.

Colchicine Ameliorates Dilated Cardiomyopathy Via SIRT2-Mediated Suppression of NLRP3 Inflammasome Activation.

Background Dilated cardiomyopathy remains a leading cause of heart failure worldwide. Immune inflammation response is recognized as a significant player in the progression of heart failure; however, immunomodulatory strategies remain a long-term challenge. Colchicine, a potent anti-inflammatory drug, has many benefits in ischemic cardiovascular events, but its role in nonischemic heart failure remains unclear.

A bioinspired hollow g-CN-CuPc heterostructure with remarkable SERS enhancement and photosynthesis-mimicking properties for theranostic applications.

Surface-enhanced Raman scattering (SERS) based on chemical mechanism (CM) has great potential for superior stability and selectivity. Moreover, a bioinspired CM-Raman substrate-Raman reporter system with charge separation and electron transport nature provides thylakoid-mimicking potential for multifunctional applications. Herein, hollow carbon nitride nanospheres hierarchically assembled with a well-oriented copper(ii) phthalocyanine layer and hyaluronic acid (HCNs@CuPc@HA) were designed as a light-harvesting nanocomposite and photosynthesis-mimicking nanoscaffold that enhance both CM-SERS and photoredox catalysis.

Correction: An engineered third electrostatic constriction of aerolysin to manipulate heterogeneously charged peptide transport.

[This corrects the article DOI: 10.1039/D1SC06459B.].

Insights into electrochemiluminescence dynamics by synchronizing real-time electrical, luminescence, and mass spectrometric measurements.

Electrochemiluminescence (ECL) comprises a sophisticated cascade of reactions. Despite advances in mechanistic studies by electrochemistry and spectroscopy, a lack of access to dynamic molecular information renders many plausible ECL pathways unclear or unproven. Here we describe the construction of a real-time ECL mass spectrometry (MS) platform (RT-Triplex) for synchronization of dynamic electrical, luminescent, and mass spectrometric outputs during ECL events.

A facile metal-free one-flask synthesis of multi-substituted furans a BF·EtO mediated formal [4 + 1] reaction of 3-chloro-3-phenyldiazirines and α,β-alkenyl ketones.

A facile, efficient and metal free one-flask approach to diversely substituted furans from easily accessible 3-chloro-3-phenyldiazirines and α,β-alkenyl ketones is reported. This protocol integrates three steps of cyclopropanation, Cloke-Wilson rearrangement and elimination of HCl in one-flask to give products in moderate to good yields. It provides a metal and oxidant free approach to multi-substituted furans with the advantages of easy operation, mild reaction conditions and a broad scope of substrates.

The contribution of new particle formation and subsequent growth to haze formation.

We investigated the contribution of atmospheric new particle formation (NPF) and subsequent growth of the newly formed particles, characterized by high concentrations of fine particulate matter (PM). In addition to having adverse effects on visibility and human health, these haze particles may act as cloud condensation nuclei, having potentially large influences on clouds and precipitation. Using atmospheric observations performed in 2019 in Beijing, a polluted megacity in China, we showed that the variability of growth rates (GR) of particles originating from NPF depend only weakly on low-volatile vapor - highly oxidated organic molecules (HOMs) and sulphuric acid - concentrations and have no apparent connection with the strength of NPF or the level of background pollution.

Sodium alkoxide-mediated g-CN immobilized on a composite nanofibrous membrane for preferable photocatalytic activity.

g-CN is a classic photocatalyst not only owing to the metal-free semiconducting electronic structure but also tunable multifunctional properties. However, strategies for chemical exfoliation of g-CN based on organic bases have been rarely reported. A family of sodium alkoxide-mediated g-CN has been prepared a simple synthesis.

A light-initiated chemical reporter strategy for spatiotemporal labeling of biomolecules.

The emergence of optochemical approaches has had a diverse impact over a broad range of biological research due to spatiotemporal regulation. Herein, we integrate this feature into the bioorthogonal chemical reporter strategy, which enables visible light-controlled spatiotemporal labeling of cell-surface glycans, lipids, and proteins. The metabolic precursors were first incorporated into live cells, and next the bioorthogonal reaction converted the azide/alkyne into a photo-active functional group, which allowed for subsequent photo-click reaction.

A reversible plasmonic nanoprobe for dynamic imaging of intracellular pH during endocytosis.

Understanding the pH evolution during endocytosis is essential for our comprehension of the fundamental processes of biology as well as effective nanotherapeutic design. Herein, we constructed a plasmonic Au@PANI core-shell nanoprobe, which possessed significantly different scattering properties under acidic and basic conditions. Encouragingly, the scattering signal of Au@PANI nanoprobes displayed a positive linear correlation with the pH value not only in PBS but also in nigericin-treated cells.

Recent advances in noble metal complex based photodynamic therapy.

Photodynamic therapy (PDT) utilizes light-activated photosensitizers (PSs) to generate toxic species for therapeutics. It has become an emerging solution for cancer treatment because of its specific spatiotemporal selectivity and minimal invasiveness. Noble metal (Ru, Ir and Pt) complexes are of increasing interest as photosensitizers for their excellent photophysical, photochemical, and photobiological properties.

A photoresponsive antibody-siRNA conjugate for activatable immunogene therapy of cancer.

Tumor-targeted delivery of small-interfering RNAs (siRNAs) for cancer therapy still remains a challenging task. While antibody-siRNA conjugates (ARCs) provide an alternative way to address this challenge, the uncontrollable siRNA release potentially leads to undesirable off-tumor side effects, limiting their therapeutic efficacy. Here, we report a photoresponsive ARC (PARC) for tumor-specific and photoinducible siRNA delivery as well as photoactivable immunogene therapy.

Endowing matrix-free carbon dots with color-tunable ultralong phosphorescence by self-doping.

Color-tunable ultralong phosphorescence is urgently desired in optoelectronic applications. Herein, we report a new type of full-color-tunable ultralong phosphorescence carbon dots (CDs) without matrix-assistance by a self-doping method under ambient conditions. The phosphorescence color can be rationally tuned from blue to red by changing the excitation wavelength from 310 to 440 nm.

Surface plasmon coupling regulated CsPbBr perovskite lasers in a metal-insulator-semiconductor structure.

A strong coupling effect often occurs between a metal and semiconductor, so micro/nano-lasers based on surface plasmons can break through the optical diffraction limit and realize unprecedented linear and nonlinear enhancement of optical processes. Hence, metal-insulator-semiconductor (M-I-S) structures based on perovskite materials were explored to design optoelectronic devices. Herein, we constructed an Ag/SiO/CsPbBr hybrid structure to generate surface plasmon coupled emission between the metal and CsPbBr perovskite.

On the photocatalysis evolution of heteroatom-doped AgM nanoclusters.

Atomically precise metal nanoclusters doped with one or more heteroatom of other metals have exhibited extraordinary catalytic properties. Here we report a series of thiolate-protected AgM (M is dopant Ni, Pd and Pt) nanoclusters that adopt a similar structural framework like a distorted hexahedron, in which four Ag atoms are located at the midpoints of four side edges and two metal heteroatoms reside on the centres of the top and the bottom planes. The opposite orders of the catalytic performances of the three catalysts for the photocatalytic degradation of the methyl orange and rhodamine B dyes are found, which is attributed to two different types of inter-molecular recombination mechanisms.

Porous hydrogen substituted graphyne as a promising anode for lithium-ion batteries.

Porous hydrogen substituted graphyne (HsGY) has been considered as a promising candidate for anode material due to its excellent electrochemical properties. In this work, we found that monolayer and bilayer HsGY are good electrodes for high charge capacity lithium-ion batteries based on density functional theory calculations. Mechanical tests reveal that monolayer and bilayer HsGY exhibit excellent mechanical properties, including large critical strains (>25%) and high in-plane stiffness (>200 N m).

Multi-shaped strain soliton networks and moiré-potential-modulated band edge states in twisted bilayer SiC.

Tuning the interlayer twist angle provides a new degree of freedom to exploit the potentially excellent properties of two dimensional layered materials. Here we investigate the structural and electronic properties of twisted bilayer SiC under a series of twist angles using first principle calculations. The interplay of interlayer van der Waals interactions and intralayer strain induces dramatic in-plane and out-of-plane displacements.