4 results match your criteria: "Nanjing University Nanjing 210023 P. R. China yilunying@nju.edu.cn.[Affiliation]"
High-throughput single biomarker identification using droplet nanopore.
Chem Sci
June 2024
Molecular Sensing and Imaging Center, School of Chemistry and Chemical Engineering, Nanjing University Nanjing 210023 P. R. China
Article Synopsis
- Biomarkers require detailed analysis at the single-molecule level for accurate clinical diagnosis, leading to the use of highly sensitive biological nanopore technology for detection.
- The development of a droplet nanopore technique allows for low-volume and high-throughput detection of biomarkers, achieving a 2000-fold reduction in sample volume compared to traditional methods.
- This new sensing platform successfully lowers detection limits to 42 picograms for biomarkers like angiotensin II, making it a strong candidate for point-of-care testing without excessive sample dilution.
Correction: An engineered third electrostatic constriction of aerolysin to manipulate heterogeneously charged peptide transport.
Chem Sci
June 2022
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University Nanjing 210023 P. R. China.
[This corrects the article DOI: 10.1039/D1SC06459B.].
View Article and Find Full Text PDFProfiling single-molecule reaction kinetics under nanopore confinement.
Chem Sci
April 2022
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University Nanjing 210023 P. R. China
The study of a single-molecule reaction under nanoconfinement is beneficial for understanding the reactive intermediates and reaction pathways. However, the kinetics model of the single-molecule reaction under confinement remains elusive. Herein we engineered an aerolysin nanopore reactor to elaborate the single-molecule reaction kinetics under nanoconfinement.
View Article and Find Full Text PDFAn engineered third electrostatic constriction of aerolysin to manipulate heterogeneously charged peptide transport.
Chem Sci
February 2022
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University Nanjing 210023 P. R. China.
Reading the primary sequence directly using nanopores remains challenging due to the complex building blocks of 20 proteinogenic amino acids and the corresponding sophisticated structures. Compared to the uniformly negatively charged polynucleotides, biological nanopores hardly provide effective ionic current responses to all heterogeneously charged peptides under nearly physiological pH conditions. Herein, we precisely design a N226Q/S228K mutant aerolysin which creates a new electrostatic constriction named R3 in-between two natural sensing regions for controlling the capture and translocation of heterogeneously charged peptides.
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