A Nitrate/Nitrite Biosensor Designed with an Antiterminator for Diagnosis of Colitis Based on .

is a common microorganism in the human gut that has been linked to health benefits. Furthermore, it is an emerging synthetic biology chassis with the potential to be modified into diagnostic or therapeutic engineered probiotics. However, the absence of biological components limits its further applications.

P/P═O Redox Catalysis Mediated Thioesterification of Carboxylic Acids with Disulfides under Air Conditions.

An efficient organophosphorus-catalyzed thiocarbonylation reaction of disulfides with carboxylic acids under air conditions was described. Various functional groups on carboxylic acids and disulfides can be tolerated under the present reaction conditions, affording thioesters in good to excellent yields. This method exhibited excellent chemoselectivity and can be applied for the late-stage functionalization of drug molecules containing a carboxylic acid group.

Pharyngeal Microbiome in Atopic Dermatitis: A 16S rRNA Sequencing Study.

While recent studies have demonstrated the involvement of the skin and gut microbiome in the pathogenesis of atopic dermatitis (AD), the influence of pharyngeal microbiota on AD remains unclear. This study aims to explore disparities in the composition of pharyngeal flora among AD patients and their potential role in the pathogenesis of AD. Between March and May 2023, 30 patients with AD at the outpatient department of Jiangsu Provincial Traditional Chinese Medicine Hospital were recruited, along with 20 healthy subjects, underwent 16S rRNA sequencing on pharyngeal swabs.

Deep learning in integrating spatial transcriptomics with other modalities.

Spatial transcriptomics technologies have been extensively applied in biological research, enabling the study of transcriptome while preserving the spatial context of tissues. Paired with spatial transcriptomics data, platforms often provide histology and (or) chromatin images, which capture cellular morphology and chromatin organization. Additionally, single-cell RNA sequencing (scRNA-seq) data from matching tissues often accompany spatial data, offering a transcriptome-wide gene expression profile of individual cells.

Dual PTCH2 mutation [Ser391*, Leu104Pro]: unveiling a potential new genetic susceptibility factor for glioma development.

Gliomas are a heterogeneous type of central nervous system tumor. The etiology of glioma formation remains elusive, with approximately 5% of gliomas being familial, underscoring the significance of understanding genetic susceptibility in glioma development. In this study, a dual germline PTCH2 mutation [Ser391*, Leu104Pro] was identified in a family with a history of glioma, and sequencing data from WES/SimcereDx Neuro-Onco 360 including 910 Chinese patients with glioma and 1666 patients with solid tumors were analyzed.

Synthetic biology meets Aspergillus: engineering strategies for next-generation organic acid production.

Organic acids constitute a vital category of chemical raw materials. They have extensive applications in industries such as polymers, food, and pharmaceuticals. Currently, industrial production predominantly relies on microbial fermentation.

Application of double-needle bi-direction barbed sutures in laparoscopic common bile duct suture: a propensity score matching analysis.

Laparoscopic exploration of the common bile duct (CBD) has emerged as a predominant method in hepatobiliary surgery owing to its minimally invasive nature and favorable outcomes compared to traditional open surgery. Suturing the CBD during laparoscopic procedures is critical for ensuring proper bile drainage and reducing postoperative complications. This retrospective study enrolled patients who underwent laparoscopic exploration of the CBD for choledocholithiasis at the Wujin Hospital Affiliated with Jiangsu University between January 2016 and December 2023.

Age-related trends in trabecular bone scores and bone mineral density in Chinese men with type 2 diabetes mellitus: a cross-sectional study.

Patients with Type 2 diabetes mellitus(T2DM) typically have an average or higher bone mineral density (BMD) but are at a significantly higher risk of fracture than patients without diabetes. Trabecular bone score (TBS) is a textural index derived from pixel gray-level variations in lumbar spine DXA image, which has been introduced as an indirect measure of bone quality. This study aimed to discuss the trends and annual rates of change in BMD and TBS with age in Chinese men with T2DM and men without diabetes mellitus.

Deep imaging of LepR stromal cells in optically cleared murine bone hemisections.

Tissue clearing combined with high-resolution confocal imaging is a cutting-edge approach for dissecting the three-dimensional (3D) architecture of tissues and deciphering cellular spatial interactions under physiological and pathological conditions. Deciphering the spatial interaction of leptin receptor-expressing (LepR) stromal cells with other compartments in the bone marrow is crucial for a deeper understanding of the stem cell niche and the skeletal tissue. In this study, we introduce an optimized protocol for the 3D analysis of skeletal tissues, enabling the visualization of hematopoietic and stromal cells, especially LepR stromal cells, within optically cleared bone hemisections.

A broadly neutralizing antibody against the SARS-CoV-2 Omicron sub-variants BA.1, BA.2, BA.2.12.1, BA.4, and BA.5.

The global spread of Severe Acute Respiratory Syndrome Coronavirus 2. (SARS-CoV-2) and its variant strains, including Alpha, Beta, Gamma, Delta, and now Omicron, pose a significant challenge. With the constant evolution of the virus, Omicron and its subtypes BA.

Treatment of a collapse fracture of the anterolateral tibial plateau with a lateral locking plate and the Jail screw technique.

Background: To explore the advantages of a lateral tibial locking plate combined with Jail screw fixation in the treatment of anterolateral tibial plateau collapse fracture (ATPCF).

Methods: A retrospective analysis was conducted on patients with ATPCFs admitted to our hospital from February 2019 to February 2023. Twenty-six patients were successfully included, including 15 males and 11 females, with an average age of 58.

Enantioselective Degradation and Processing Factors of Seven Chiral Pesticides During the Processing of Wine and Rice Wine.

Chiral pesticides often undergo enantioselective degradation during food fermentation. In this study, the enantioselective fates of seven chiral pesticides during processing of wine and rice wine were investigated. The results revealed that R-metalaxyl, R-mefentrifluconazole and S-hexaconazole were preferentially degraded during wine processing with EF values of 0.

Evaluation of Multispecific Drugs Based on Patient-derived Immunocompetent Tumor Organoids.

The evolution of antitumor drug development has transitioned from single-agent chemotherapy to targeted therapy, immunotherapy, and more recently, multispecific drugs. These innovative drugs target multiple cellular or molecular pathways simultaneously, offering a more comprehensive anticancer approach and addressing some of the limitations inherent in traditional monotherapies. However, preclinical assessment of multispecific drugs remains challenging, as conventional tumor models often lack the necessary complexity to accurately reflect the interactions between various cell types and targets.

1.5D Chiral Perovskites Mediated by Hydrogen-Bonding Network with Remarkable Spin-Polarized Property.

In this study, we developed new chiral hybrid perovskites, (R/S-MBA)(GA)PbI4, by incorporating achiral guanidinium (GA+) and chiral R/S-methylbenzylammonium (R/S-MBA+) into the perovskite framework. The resulting materials possess a distinctive structural configuration, positioned between 1D and 2D perovskites, which we describe as 1.5D.

Tumor cell-derived N-acetyl-aspartyl-glutamate reshapes the tumor microenvironment to facilitate breast cancer metastasis.

Neurotransmitters are increasingly recognized to play important roles in limiting anti-tumor immunity. N-acetyl-aspartyl-glutamate (NAAG) has been extensively studied in neurological disorders; however, its potential role in restricting anti-tumor immunity has not been investigated. Here, we demonstrated that NAAG or its synthetase RimK-like family member B (RIMKLB) significantly disrupted anti-tumor immunity by rewiring the myeloid progenitor differentiation of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs), which in turn promoted breast cancer growth and metastasis.

Quality Evaluation of Wenyujin Rhizoma Concisum From Different Districts in China Based on HPLC, Heracles NEO Ultrafast Gas-Phase Electronic Nose, and FT-NIR.

Introduction: Wenyujin Rhizoma Concisum, named as Pian Jianghuang (PJH) in China, is the decoction piece from the dried rhizome of Curcuma wenyujin Y. H. Chenet C.

Characterization of Quality Marker for Mume Fructus Pulp by Ultra-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry-Based Untargeted Metabolomics Combined With Biological Activities.

Introduction: As a widely used Chinese herbal medicine, Mume Fructus pulp (MFP) has rich nutritional value and biological activity, but its quality control research is relatively scarce.

Objectives: The objective of the study was to evaluate the quality difference between MFPs from different origins and its adulterant apricot pulp (APP), and to identify potential quality markers.

Methods: The chemical compositions were identified by untargeted metabolomics analysis based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry combined with feature-based molecular networking.

An overview of recent progress in cultured meat: focusing on technology, quality properties, safety, industrialization and public acceptance.

Cultured meat technology represents an innovative food production approach that enables the large-scale cultivation of animal cells to obtain muscle, fat, and other tissues, which are then processed into meat products. Compared to traditional meat production methods, cell-cultured meat may significantly reduce energy consumption by 7% to 45%, greenhouse gas emissions by 78% to 96%, land use by 99%, and water use by 82% to 96%. This technology offers several advantages, including a shorter production cycle and enhanced environmental sustainability, resource efficiency, and overall sustainability.

How hydrodynamic conditions drive the regime shift towards a bacterial state with lower carbon emissions in river bends.

Hydrodynamic conditions influenced by river sinuosity may alter carbon (e.g., carbon dioxide and methane) emissions and microbial communities responsible for nutrient turnover.

Spatio-temporal variations of heat extremes across the yangtze river delta during 2001-2023 based on remotely sensed seamless air temperature.

Heat extremes become increasingly frequent and severe, posing adverse risks to public health and environment. Previous research on extreme heat mostly used meteorological observations or reanalysis data, which cannot well capture detailed spatial patterns. This study developed a seamless air temperature (T) dataset from remote sensing data to characterize the spatio-temporal variations of heat extremes in the Yangtze River Delta (YRD) from 2001 to 2023.

Iron-carbon micro-electrolysis coupled to heterotrophic nitrification aerobic denitrification treating low carbon/nitrogen mariculture wastewater.

Considering the unsatisfied nitrogen (N) and phosphorus (P) treatment performance of mariculture wastewater caused by low carbon/nitrogen (C/N), a novel iron-carbon (Fe-C) micro-electrolysis coupled to heterotrophic nitrification aerobic denitrification (HNAD) process was proposed to enhance the N and P elimination. Results revealed that total nitrogen (TN) removal and total phosphorus (TP) removal efficiencies in Fe-C filter with HNAD (R-Fe) increased by 76.1% and 113.

Microporous carbon derived from waste plastics for efficient adsorption of tetracycline: Adsorption mechanism and application potentials.

In recent years, the accumulation of waste plastics and emergence plastic-derived pollutants such as microplastics have driven significantly the development and updating of waste plastic utilization technology. This study prepared the porous carbon (PC-1-KOH) material directly from polyethylene terephthalate (PET) in waste plastic bottles using KOH activation and molten salt strategy for efficient removal of antibiotic tetracycline (TC). The maximum removal efficiency of TC was 100.

Data-Driven Fault Detection and Diagnosis Methods in Wastewater Treatment Systems: A Comprehensive Review.

Wastewater treatment systems are essential for sustainable water resource management but face challenges such as equipment and sensor malfunctions, fluctuating influent conditions, and operational disturbances that compromise process stability and detection accuracy. To address these challenges, this paper systematically reviews data-driven fault detection and diagnosis (FDD) methods applied in wastewater treatment systems from 2014 to 2024, focusing on their applications, advancements, and limitations. Main contributions include an overview of key treatment processes, a detailed evaluation of fault types (process and sensor faults), advancements in multivariate statistical methods, machine learning (ML), and hybrid FDD techniques, as well as their effectiveness in anomaly detection, managing complex data distributions, and enabling real-time monitoring.

Danggui Buxue Decoction Regulates Autophagy to Improve Renal Fibrosis in diabetes through miR-27a /PI3K/AKT pathway.

Ethnopharmacological Relevance: Danggui Buxue Decoction (DBD) is a classic traditional Chinese herbal formulation, composed of Astragali Radix (AR) and Angelica Sinensis Radix (ASR) in a ratio of 5:1. It is a traditional Chinese medicine classic prescription for nourishing Qi and Yin (vital energy and body fluids), and it is effective in treating various clinical diseases. Diabetic nephropathy (DN) is categorized under "thirsting," "edema," and "turbid urine" in Traditional Chinese Medicine (TCM).

From natural herbal wisdom to nano innovation: Revolutionizing tumor treatment through metabolic reprogramming.

In recent years, with the deepening understanding of the biological mechanisms underlying tumorigenesis, metabolic reprogramming has emerged as a pivotal process in cancer initiation, progression, and treatment resistance, gradually paving the way for new avenues in precision oncology. Natural herb ingredients, characterized by their multi-target engagement, low toxicity, and wide-ranging biological activities, exhibit unique advantages in anti-cancer therapy. Nonetheless, the clinical application of these components has been constrained by issues such as poor solubility, low bioavailability, and inadequate stability when administered through traditional delivery methods.