Improved dialysis removal of protein-bound uremic toxins by salvianolic acids.

Background: Indoxyl sulfate (IS) and p-cresyl sulfate (pCS) are two key protein-bound uremic toxins that accumulate in patients with end-stage renal disease. IS and pCS cannot be efficiently removed by conventional hemodialysis because they are highly bound to proteins. One promising means to optimize the removal of protein-bound uremic toxins involves using binding competitors to liberate uremic toxins from protein-binding partners.

[Acupuncture Treatment Reduces Body Weight Possibly by Down-regulating Insulin and Leptin Resistance, and Up-regulating Soluble Leptin Receptor Level in Prediabetic Patients].

Objective: To observe the effect of acupuncture of Feishu (BL 13), Pishu (BL 20), etc. on body weight, blood lipid, leptin (LP) and soluble leptin receptor (sOB-R) levels in prediabetic patients, so as to explore its mechanisms underlying treatment and prevention of prediabetes.

Methods: A total of 100 prediabetic patients were randomly assigned to medication (Metformin) group (＝35), acupuncture group (＝35) and simple lifestyle intervention group (＝30) with random number table method.

[Mucinous tubular and spindle cell carcinoma of kidney: a clinicopathological study].

Objective: To investigate the clinicopathological features, histogenesis and prognosis of mucinous tubular and spindle cell carcinoma (MTSCC).

Methods: Five MTSCCs were studied with histochemical, immunohistochemical staining, electron microscopy, and review of the related literatures.

Results: Four cases of MTSCC were females and one was male.

[Expression of CD20 in thymomas and its clinical implication].

Objective: To study the expression of CD20 in thymomas and its clinical significance.

Methods: One hundred and seventy-nine cases of thymoma were enrolled into the study. The histologic diagnosis was reviewed by two experienced pathologists on the basis of the 2004 WHO classification.

[Clinicopathologic and molecular genetic study of renal cell carcinoma occurring in teenagers].

Objective: To investigate clinicopathological features, molecular genetic characteristics, differential diagnoses and prognosis of renal cell carcinoma in teenagers.

Methods: Microscopic and immunohistochemical features of 46 cases of renal cell carcinomas in teenagers were reviewed along with the clinical follow-up data. Loss of heterozygosity (LOH), analysis of von Hippel-Lindau (VHL) gene and screening for VHL gene mutations were performed in all of the tumors.

[Early clinical manifestations of osteogenic sarcoma].

Objective: To investigate early clinical manifestations of osteogenic sarcoma to help establishment of an early diagnosis of the disease.

Methods: A total of 92 patients with osteogenic sarcoma in the extremities were admitted to our hospital from April 1984 to October 2002. Of the 92 patients, 71 (42 males and 29 females; averaged age 17.

[An analysis on follow-up of the treatment of complicated giant cell tumor of bone].

Objective: To discuss the definition of complicated giant cell tumor of the bone and retrospectively analyze the treatment protocols and their therapeutic results so as to provide a clinical basis for reducing the postoperative recurrence of this kind of tumor.

Methods: From April 2001 to April 2005, 22 patients (11 males and 11 females, aged 15-66 years) with complicated giant cell tumor of the bone were treated by the marginal or wide excision. The tumor was located in the distal femur in 10 patients, the proximal tibia in 5, the proximal femur in 2, the proximal humerus in 2, the hip bone in 2, and the distal radius in 1.

[Limb salvage with osteoarticular allografts after resection of proximal tibia bone].

Objective: To evaluate the short-term efficacy of osteoarticular allografts in the limb salvage of the proximal tibia.

Methods: From 1998 to 2003, 15 patients (7 males, 8 females; aged 14-56 yr. average 33) with bone tumor of the proximal tibia underwent osteoarticular allografts.

[Surgical management of chronic radiation enteritis].

Objective: To explore the surgical methods and the clinical results of chronic radiation enteritis.

Methods: Treatments were applied to forty-nine cases of chronic radiation enteritis complicated with intestinal obstruction, enterocutaneous fistula, intestinal stenosis, intestinal bleeding, severe proctocolitis and intestinal perforation, among whom 47 cases received an average of 2.8 +/- 2.

[Effect of thymosin alpha1 on immunological function and metabolism in peritoneal sepsis rats].

Objective: To investigate the effect of thymosin alpha1 on immunological function and protein metabolism in peritoneal sepsis rats.

Methods: We observed the effect of thymosin alpha1 on T cell subclassification, TNFalpha, IL-6, IL-10, albumin, C-reactive protein (CRP) and mortality in cecal ligation and puncture (CLP) induced septic rats.

Results: Concentration of TNFalpha and IL-6 in CLP induced septic rats increased significantly, and concentration of IL-10 decreased significantly compared to control group.

[Expression of survivin in human gastric adenocarcinomas: correlation with proliferation and apoptosis].

Objective: To investigate the expression and significance of survivin, ki-67 and apoptosis index in patients with advanced gastric adenocarcinoma.

Methods: Immunohistochemical SP method for survivin expression as well as cell proliferative index (ki-67) and apoptosis index (TUNEL) was conducted on 120 gastric adenocarcinomas.

Results: The survivin was detected in the cytoplasm of carcinoma cells in 59 (49.

[Non-composite combined liver and intestinal allotransplantation].

Objective: To report the first case of non-composite combined liver and intestinal allotransplantation in China. The technical aspects of the case and pros and cons of such an approach versus composite technique were discussed.

Methods: The patient suffered from short bowel syndrome and TPN-related liver damage.

[Albumin kinetics in patients with severe sepsis].

Objective: To explore the mechanism of hypoalbuminemia in patients with severe sepsis.

Methods: I(125)-labeled albumin was administered intravenously to 10 health volunteers and 10 patients with severe sepsis. Blood samples were taken at 0, 1, 2, 4, 8, 12, 24 hours and 2, 3, 4, 5, 6, 7, 9, 11, 13, 15, 18, 22, 25 days for the measurement of the dose of gamma-radiation and the curve of concentration and time.

[Operative stress response and energy metabolism after laparoscopic cholecystectomy and open cholecystectomy].

Objective: To determine the level of neurohormonal operative stress response-reactive protein (CRP) and rest energy expenditure (REE) after laparoscopic cholecystectomy (LC) and open cholecystectomy (OC).

Methods: Twenty-six consecutive patients with noncomplicated gallstones were randomized for LC (n = 14) and OC (n = 12). Plasma concentrations of somatotropin, insulin, cortisol and CRP were measured.

Growth hormone improves graft mucosal structure and recipient protein metabolism in rat small bowel transplantation.

Objective: To observe the effects of recombinant human growth hormone (rhGH) on graft structure and recipient protein metabolism in rat small bowel transplantation (SBT) and total parenteral nutrition (TPN) models.

Methods: Twenty recipients of rat allogeneic heterotopic small bowel transplants (SD-->Wistar) were divided into two groups (GH group and control group). Both groups were supported by standard TPN.

Rehabilitative therapy of short bowel syndrome: experimental study and clinical trial.

Objectives: To investigate the effect of growth hormone on proliferative activity of the residual small intestinal mucosa after massive small intestinal resection and to evaluate the clinical efficacy of bowel rehabilitative therapy for short bowel syndrome.

Methods: Small intestinal mucosa proliferative activity were compared in rats from control group (sham operation), short bowel group (80% small bowel resection) and growth hormone treatment group (80% small bowel resection + growth hormone 1 U x kg(-1) x d(-1) for 28 days) with the aid of histology image analysis, flow cytometric assay, immunohistochemistry analysis and RT-PCR assay. The nutritional status, D-xylose absorption and stool nitrogen output were observed in 9 consecutive parenteral nutrition dependent patients with short bowel syndrome after intestinal rehabilitative therapy (growth hormone 8 - 12 U x kg(-1) x d(-1) im + glutamine 0.

[Relationship between insulin-like growth factor II and prognosis of colorectal cancer].

Objective: To investigate the relationship between insulin-like growth factor II (IGF-II) and prognosis of colorectal cancer.

Methods: One hundred and forty-two colorectal cancer patients were enrolled. In colonoscopic biopsy specimens, the expression of IGF-II and PCNA were detected immunohistochemically, while TUNEL technique was used to detect apoptosis.

[The proper opportunity of surgery for rectal cancer after preoperative adjuvant chemotherapy].

Objective: To discuss the proper opportunity of surgery for rectal cancer after preoperative intra-arterial chemotherapy.

Methods: Preoperative chemotherapy was performed on 27 rectal cancer cases at Dukes stage B and C. The therapy comprised of arterial infusion of 5-fluorouracil 600 mg/m(2), mitomycin 15 mg/m(2) and epirubicin 30 mg/m(2) for one course.

[Endovascular treatment of carotid-cavernous sinus fistulae].

Objective: To treat carotid-cavernous sinus fistulae mmunications between 43 patients with endovascular procedure.

Method: Of the 43 patients 37 were male and 6 female. Traumatic and spontaneous CCF were noted in 39 and 4 patients respectively.

[Detection of bacterial DNA from cholesterol gallstones by NP-PCR and its clinical significance].

To search for bacterial DNA sequences in cholesterol gallstones with negative bacterial culture. We used nested primers polymerase chain reaction (NP-PCR) technique to amplify bacterial gene fragments were amplified in vitro from DNA extracted from cholesterol gallstones. Comparative 16S ribosomal RNA sequence analysis was used for elucidation of bacterial identification.

[The diagnosis and surgical treatment in rectal endometriosis].

From Jun 1975 to Mar 1995, 26 cases of endometriosis in the rectum (RE) were admitted. Local resection was performed in 16 and Dixon's operation in 10. The result showed 21 cases were symptomatically cured and 5 with remission.

[Influence of recombinant growth hormone on protein metabolism during severe infection: an animal experiment].

We observed the influence of recombinant growth hormone (rGH) on protein metabolism during sepsis and found its mechanisms. Cecal ligation and puncture were choosen to duplicate the severe infection model. Animals of therapy group received rGH 1 U/kg/d after CLP operation, while sepsis group received normal saline.

[Molecular mechanism and therapy of hypoalbuminemia in peritoneal infection].

Albumin mRNA expression was studied by the use of reverse transcriptional polymerase chain reaction (RT-PCR) to determine the molecular mechanism in peritoneal infection. Albumin mRNA content markedly decreased. Endotoxin inhibited albumin mRNA expression in vivo probably by stimulating TNF,IL-1, and IL-6 production.

[Clinical features and CT diagnostic criteria for diffuse axonal brain injury].

The clinical and computed tomographic features of 117 patients with severe closed head injury were analysed. We put forward the CT diagnostic criteria of diffuse axonal injury (DAD)i.e.

[Orthotopic heart transplantation: a case report].

In July 1994, we performed orthotopic heart transplantation for a patient with hypertrophic myocardiopathy, who had had open heart surgery enlarging the right ventricular outlet. The patient had an excellent postoperative result. He received triple-drug immunosuppressive therapy.