Separation of flavonoids with significant biological activity from leaves.

leaves, which are a rich source of flavonoids, were used to separate and purify myricitrin (W3) and myricetin-3--glucoside (W1). Further, the antioxidant and hypoglycemic activities of the two purified flavonoids were evaluated. The flavonoids were separated using solvent partition, macroporous adsorbent resin column, and Sephadex column chromatography, and purified using preparative reverse-phase high-performance liquid chromatography (HPLC).