DNAcycP2: improved estimation of intrinsic DNA cyclizability through data augmentation.

Loop-seq is a pioneering high-throughput assay that enables the simultaneous quantification of intrinsic cyclizability across a large set of DNA fragments. However, the assay's reliance on biotin-tethered elongated DNA fragments introduces a tethering effect, leading to biased cyclizability measurements. We demonstrate that the current de-biasing technique is inadequate for fully mitigating this bias.

Midterm Evaluation of EUS-guided Gastroenterostomy for Gastric Outlet Obstruction: An International Collaborative Study.

Background: EUS-guided gastroenterostomy (EUS-GE) is a minimally invasive therapy for the management of gastric outlet obstruction (GOO). EUS-GE has demonstrated excellent short-term efficacy without the risks of surgical bypass. However, there is limited data on follow-up outcomes.

Social-Environmental Constraints on the Development of a Concealable Stigmatized Identity Predict Psychological Distress.

People who are stigmatized along concealable features (e.g., individuals reporting adverse childhood experiences) often experience challenges to the self-concept, which can promote psychological distress.

Designing Baby-Friendly Hospital Step 2 Training A Hospital Case Study.

Maintaining Baby-Friendly Hospital Initiative (BFHI) standards within a complex healthcare system presents unique challenges. This case study from a regional perinatal center in the northeast United States details the design and implementation of a program to address BFHI Step 2, which requires ongoing competency assessment and team member training to ensure breastfeeding support. The shift of BFHI competencies to continuous professional development introduced logistical challenges, compounded by staff turnover and budget constraints.

Transaction between impulsivity and family conflict among children: An empirical examination of the biosocial model of emotion regulation.

Difficulty with emotion regulation is a transdiagnostic problem associated with a variety of psychological disorders. The biosocial model suggests that early biological vulnerability, including impulsivity, may potentiate across development by transacting with environmental risk factors leading to the development of emotional dysregulation. During transition from late childhood to early adolescence, family may be a prominent source of environmental influences.

Association between behavioural risk factors for hypertension and concordance with the Dietary Approaches to Stop Hypertension dietary pattern among South Asians in the Mediators of Atherosclerosis in South Asians Living in America (MASALA) study.

South Asians are among the fastest-growing immigrant population group in the United States (U.S.) with a unique disease risk profile.

MechanoBioCAD: a generalized semi-automated computational tool for mechanobiological studies.

Soft micropillar arrays enable detailed studies of cellular mechanotransduction and biomechanics using traditional beam-bending models. However, they often rely on simplified assumptions, leading to significant errors in force estimation. We present MechanoBioCAD (MBC), a finite element method (FEM)-based tool designed specifically for micropillar research and error estimation.

EXPRESS: Cancer Mortality in Patients with Systemic Lupus Erythematosus.

Systemic lupus erythematosus (SLE) is linked with an increased risk of cancers, particularly hematologic malignancies. Cancer mortality among patients with SLE in the United States (U.S.

Threatened synanthropes depend on intact forests: a critical evaluation of Moore et al. (2023).

Synanthropes are known for their remarkable adaptability to coexist with humans, yet increased visibility exposes them to significant threats, such as hunting or conflict over resources. Moore et al.'s review 'The rise of hyperabundant native generalists threatens both humans and nature' (https://doi.

Endophytic bacteria discovered in oil body organelles of the liverworts Marchantia polymorpha and Radula complanata.

Premise: Interactions between endophytic microbes and bryophytes have been understudied. The liverwort oil body has also remained poorly understood since its discovery, and modern studies have failed to ascertain its function and composition. Many liverwort species possess oil bodies with conspicuous granules of unknown structure.

Independent Risk Factors for Less Than "Top Box" Doctor Communication Patient Experience Survey Scores in an Urban Teaching Hospital.

This study explored the independent risk factors for patients giving a less than "top box" score on their Hospital Consumer Assessment of Healthcare Providers and Systems (HCAHPS) surveys in the "doctor communication" domain (DCD). After adjusting for age, gender, length of stay, receiving a postdischarge phone call (PC), new medication during hospitalization, highest education level, language spoken at home, and zip code, results from 803 HCAHPS surveys (from January 1 to December 31, 2023) revealed that not receiving a PC was associated with a more than doubling of the odds that a patient would give less than a "top box" score for the DCD (overall adjusted odds ratio of 2.22; 95% confidence interval: 1.

Molecular determinants of response to neoadjuvant pembrolizumab plus chemotherapy in patients with high-risk, early-stage, triple-negative breast cancer: exploratory analysis of the open-label, multicohort phase 1b KEYNOTE-173 study.

Background: The multicohort, open-label, phase 1b KEYNOTE-173 study was conducted to investigate pembrolizumab plus chemotherapy as neoadjuvant therapy for triple-negative breast cancer (TNBC). This exploratory analysis evaluated features of the tumor microenvironment that might be predictive of response.

Methods: Cell fractions from 20 paired samples collected at baseline and after one cycle of neoadjuvant pembrolizumab prior to chemotherapy initiation were analyzed by spatial localization (tumor compartment, stromal compartment, or sum of tumor and stromal compartments [total tumor]) using three six-plex immunohistochemistry panels with T-cell, myeloid cell, and natural killer cell components.

Dys-regulated phosphatidylserine externalization as a cell intrinsic immune escape mechanism in cancer.

The negatively charged aminophospholipid, phosphatidylserine (PS), is typically restricted to the inner leaflet of the plasma membrane under normal, healthy physiological conditions. PS is irreversibly externalized during apoptosis, where it serves as a signal for elimination by efferocytosis. PS is also reversibly and transiently externalized during cell activation such as platelet and immune cell activation.

Clinician differences in attitudes and perceptions on the use of long-acting injectable antipsychotic agents in treating patients with schizophrenia: results from the US DECIDE survey.

Background: Long-acting injectable antipsychotics (LAIs) reduce relapses in schizophrenia; however, most clinicians reserve LAIs for nonadherence with oral antipsychotics (OAs) or severe disease.

Methods: US psychiatric clinicians were surveyed regarding their schizophrenia management practices and use of LAIs. Respondents were grouped by LAI use (high [≥ 31% of patients using LAIs], low [≤ 14% using LAIs]; mid not analyzed) and mindset based on their response to "Which of the following best fits the current way you view your use of [LAIs] for your patients with schizophrenia?"

Results: Respondents (n = 380) were distributed across LAI use (106 high, 130 low) and mindset (123 early-use, 88 severity-reserved, 113 adherence-reserved, 56 LAI-hesitant) subgroups.

PTN activity in quiescent neural stem cells mediates Shank3 overexpression-induced manic behavior.

Mania is a complex psychiatric disease characterized by hyperactivity, elevated mood and reduced anxiety. Despite extensive studies on the mechanism of the manic episodes, the molecular targets that control manic pathogenesis remain largely unclear. Here, through single-cell RNA sequencing (scRNA-seq) analysis, we show aberrant adult neurogenesis due to increased numbers of quiescent neural stem cells (qNSC) in a manic mouse model with Shank3 overexpression.

In Vitro-In Vivo Correlation Of Amorphous Solid Dispersion Enabled Itraconazole Tablets.

Purpose: There are scarce reports on in vitro-in vivo correlation (IVIVC) model development of immediate-release (IR) formulations, and few investigations of the impacts of formulation and process of spray-dried solid dispersions (SDD)-based tablets on human pharmacokinetics (PK), despite commercial product successes. The goal of this study was to investigate the formulation and process factors that impact bioavailability enhancement of IR itraconazole SDD tablets; and to develop an FDA level A IVIVC that would predict in vivo PK performance from in vitro dissolution testing.

Methods: A direct, differential-equation-based IVIVC model approach was employed, using an oral solution for post-dissolution disposition and Fast-, Medium-, and Slow-release tablets.

A patient first perspective of sleep disturbance across therapeutic areas: a systematic literature review of qualitative studies.

Background: Sleep, a vital pillar of health, impacts daily functioning and overall quality of life. Despite extensive research on sleep disturbances within specific therapeutic areas (TAs), there's limited understanding of how these disturbances affect patients across multiple TAs. This systematic literature review adopts a patient-centered approach to explore the meaningful aspects of health and concepts of interest relating to sleep and sleep disturbances across a variety of TAs, utilizing qualitative research to bridge the gap between patient experiences and clinical measures.

PD-1 transcriptomic landscape across cancers and implications for immune checkpoint blockade outcome.

Programmed cell death protein 1 (PD-1) is a critical immune checkpoint receptor and a target for cancer immune checkpoint inhibitors (ICI). We investigated PD-1 transcript expression across cancer types and its correlations to clinical outcomes. Using a reference population, PD-1 expression was calculated as percentiles in 489 of 514 patients (31 cancer types) with advanced/metastatic disease.

Lactobacillus acidophilus (strain Scav) Postbiotic Metabolites Reduce Infection and Modulate Inflammation in an in vivo model of Pseudomonas aeruginosa wound infection.

Aims: This study assessed the antibacterial, anti-biofilm, and immunomodulatory activity of Lactobacillus acidophilus (strain Scav) postbiotic (LaP) in a mouse model of Pseudomonas aeruginosa wound infection and evaluated the bioactive components of the LaP.

Methods And Results: LaP was tested for P. aeruginosa clearance and immunomodulatory activity during wound infection.

Automated versus Manual Femoral Component Rotation Planning in Robotic-Assisted and Conventional Total Knee Arthroplasty: A Retrospective Comparison.

Background: The purpose of this study was to determine if using automated femoral rotation planning in robotic-assisted total knee arthroplasty (RA-TKA) was associated with differences in functional outcomes compared to patients who underwent manually set femoral rotation in RA-TKA or conventional TKA (C-TKA).

Methods: This was a retrospective, multi-center study of patients who underwent TKA utilizing conventional methods with femoral component rotation set to three degrees externally (C-TKA) [n = 108 knees], RA-TKA with automated femoral rotation planning intrinsic to the system (A-RA-TKA) [n = 111], and RA-TKA with femoral rotation manually set by the surgeon (M-RA-TKA) [n = 152], at least one year before follow-up. Outcome measures included the range of motion (ROM), Knee Injury and Osteoarthritis Joint Replacement (KOOS-JR), and Forgotten Joint Score (FJS).

Relations of temporoparietal connectivity with neighborhood social fragmentation in youth at clinical high-risk for psychosis.

Temporoparietal brain areas comprise a candidate set of regions for interrogating the brain functional correlates of socioenvironmental factors in people at clinical high-risk for psychosis (CHR-P). Temporal lobe abnormalities have been shown to be common among people with schizophrenia spectrum conditions. Further, temporoparietal brain regions are implicated in tasks relevant to psychosocial outcomes, including coherent autobiographical memory recall and multimodal integration.

Real-World Treatment Patterns and Outcomes in Patients With Bacillus Calmette-Guérin-Unresponsive High-Risk Non-Muscle-Invasive Bladder Cancer: A Multicountry Medical Chart Review.

Introduction: Treatment patterns for patients with bacillus Calmette-Guérin (BCG)-unresponsive high-risk non-muscle-invasive bladder cancer (NMIBC) who are ineligible for or decline radical cystectomy (RC) are inconsistently reported. We retrospectively described demographic, clinical, and treatment characteristics for these patients and assessed their clinical outcomes.

Patients And Methods: Medical charts of patients with BCG-unresponsive high-risk NMIBC (carcinoma in situ [cohort A] or T1/high-grade Ta [cohort B]) who were ineligible for or declined RC documented between January 1, 2011, and December 31, 2018, at 15 academic centers were reviewed.