Immobilizing calcium-dependent affinity ligand onto iron oxide nanoparticles for mild magnetic mAb separation.

Current downstream processing of monoclonal antibodies (mAbs) is limited in throughput and requires harsh pH conditions for mAb elution from Protein A affinity ligands. The use of an engineered calcium-dependent ligand (Z) in magnetic separation applications promises improvements due to mild elution conditions, fast processability, and process integration prospects. In this work, we synthesized and evaluated three magnetic nanoparticle types immobilized with the cysteine-tagged ligand Z-cys.

Micro-jet formation induced by the interaction of a spherical and toroidal cavitation bubble.

We investigate experimentally and numerically the interaction between a spherical cavitation bubble and a wall-bounded toroidal cavitation bubble. We demonstrate that shock wave focusing following toroidal bubble initiation induces the formation of micro-jets that pierce the spherical bubble in the torus-axis direction away from the surface, strongest in the anti-phase scenario. The velocity of micro-jets is determined by the initial standoff distance of the spherical bubble from the wall and thus from the toroidal bubble, with peak jet velocities approaching 1000m/s.

Influence of magnetic convection on separation efficiency in magnetophoretic microfluidic processes: a combined simulation and experimental study.

This work explores the complex hydrodynamics in magnetophoretic microfluidic processes, focusing on the interplay of forces and particle concentrations. The study employs a combined simulation and experimental approach to investigate the impact of magnetophoresis on magneto-responsive nanoparticles (MNPs) and their environment, including non-magneto-responsive nanoparticles (non-MNPs) in a microfluidic system. Our findings reveal that the motion of MNPs induces a hydrodynamic convective motion of non-MNPs, significantly affecting the separation efficiency and purity of the particles.

Vibrio natriegens: Application of a Fast-Growing Halophilic Bacterium.

The fast growth accompanied with high substrate consumption rates and a versatile metabolism paved the way to exploit Vibrio natriegens as unconventional host for biotechnological applications. Meanwhile, a wealth of knowledge on the physiology, the metabolism, and the regulation in this halophilic marine bacterium has been gathered. Sophisticated genetic engineering tools and metabolic models are available and have been applied to engineer production strains and first chassis variants of V.

Flow field data of three-dimensional Riemann problems.

Common validation and verification test cases for compressible flow solvers are only one- or two-dimensional. Such flows, however, are inherently three-dimensional. The provided data contains simulation results of genuine three-dimensional Riemann problems computed with the open-source compressible flow solver ALPACA.

Bacterial reduction and temperature increase of titanium dental implant models treated with a 445 nm diode laser: an in vitro study.

In this in vitro study, the use of a 445 nm diode laser was investigated for the decontamination of titanium dental implants. Different irradiation protocols and the effect of repetitive laser irradiation on temperature increase and decontamination efficacy were evaluated on titanium implant models. An automated setup was developed to realize a scanning procedure for a full surface irradiation to recapitulate a clinical treatment.

Direct Affinity Ligand Immobilization onto Bare Iron Oxide Nanoparticles Enables Efficient Magnetic Separation of Antibodies.

Magnetic separation is a promising alternative to chromatography for enhancing the downstream processing (DSP) of monoclonal antibodies (mAbs). However, there is a lack of efficient magnetic particles for successful application. Aiming to fill this gap, we demonstrate the suitability of bare iron oxide nanoparticles (BION) with physical site-directed immobilization of an engineered Protein A affinity ligand (rSpA) as an innovative magnetic material.

Millifluidic magnetophoresis-based chip for age-specific fractionation: evaluating the impact of age on metabolomics and gene expression in yeast.

A novel millifluidic process introduces age-based fractionation of var. yeast culture through magnetophoresis. yeast is a model organism for aging research used in various industries.

Amplification of Supersonic Microjets by Resonant Inertial Cavitation-Bubble Pair.

We reveal for the first time by experiments that within a narrow parameter regime, two cavitation bubbles with identical energy generated in antiphase develop a supersonic jet. High-resolution numerical simulation shows a mechanism for jet amplification based on toroidal shock wave and bubble necking interaction. The microjet reaches velocities in excess of 1000  m s^{-1}.

Development of a New Affinity Gold Polymer Membrane with Immobilized Protein A.

New and highly selective stationary phases for affinity membrane chromatography have the potential to significantly enhance the efficiency and specificity of therapeutic protein purification by reduced mass transfer limitations. This work developed and compared different immobilization strategies for recombinant Protein A ligands to a gold-sputtered polymer membrane for antibody separation in terms of functionalization and immobilization success, protein load, and stability. Successful, functionalization was validated via X-ray photoelectron spectroscopy (XPS).

Dopamine-Mediated Biopolymer Multilayer Coatings for Modulating Cell Behavior, Lubrication, and Drug Release.

Biopolymer coatings on implants mediate the interactions between the synthetic material and its biological environment. Owing to its ease of preparation and the possibility to incorporate other bioactive molecules, layer-by-layer deposition is a method commonly used in the construction of biopolymer multilayers. However, this method typically requires at least two types of oppositely charged biopolymers, thus limiting the range of macromolecular options by excluding uncharged biopolymers.

A Mucin-Based Bio-Ink for 3D Printing of Objects with Anti-Biofouling Properties.

With its potential to revolutionize the field of personalized medicine by producing customized medical devices and constructs for tissue engineering at low costs, 3D printing has emerged as a highly promising technology. Recent advancements have sparked increasing interest in the printing of biopolymeric hydrogels. However, owing to the limited printability of those soft materials, the lack of variability in available bio-inks remains a major challenge.

General mechanisms for stabilizing weakly compressible models.

Many weakly compressible models with intrinsic mechanisms for stabilizing computation have been proposed to simulate incompressible flows. The present paper analyzes several weakly compressible models to establish general mechanisms that incorporate them into a unified and simple framework. It is found that all these models contain some identical numerical dissipation terms, mass diffusion terms in the continuity equation, and bulk viscosity terms in the momentum equation.

Analysis and reconstruction of the multiphase lattice Boltzmann flux solver for multiphase flows with large density ratios.

The multiphase lattice Boltzmann flux solver (MLBFS) has been proposed to tackle complex geometries with nonuniform meshes. It also has been proven to have good numerical stability for multiphase flows with large density ratios. However, the reason for the good numerical stability of MLBFS at large density ratios has not been well established.