2 results match your criteria: "Mucopolysaccharidoses Types I-VII"
Diagnostic screening for mucopolysaccharidoses types I-VII by fluorophore-labelled carbohydrate PAGE.
J Inherit Metab Dis
October 1996
Kaiser Permanente Regional Metabolic Laboratory, North Hollywood 91601, USA.
Optimization of electroporation for transfection of human fibroblast cell lines with origin-defective SV40 DNA: development of human transformed fibroblast cell lines with mucopolysaccharidoses (I-VII).
Cell Struct Funct
April 1992
Department of Pediatrics, Gifu University School of Medicine, Japan.
Article Synopsis
- The study aimed to improve the transfection process of human fibroblasts using a specific type of SV40 DNA, focusing on optimizing experimental conditions like voltage and pulse duration.
- The best results came from applying 2 or 3 electric pulses at voltages between 1500 to 2000 V, which successfully produced 10 to 30 transformed cell foci after 6 weeks.
- The researchers created rapidly growing fibroblast cell lines that maintained crucial genetic features and enzyme deficiencies, including a specific mutation connected to mucopolysaccharidosis type VII.