Matrix metalloproteinases and inflammatory cytokines in oral fluid of patients with chronic generalized periodontitis and various construction materials.

We present the results of comparative enzyme-linked immunosorbent assay of matrix metalloproteinases MMP-2, MMP-8, MMP-9; IL-1β and IL-6; tissue inhibitors of MMP (TIMP-1, TIMP-2), and TNF-α. The object of study was oral fluid of practically healthy subjects with intact periodontium and patients with chronic generalized periodontitis with various structural materials of dental restorations. It was found that MMP-9 in oral fluid could be considered as a marker of chronic generalized periodontitis irrespective of the presence or absence of metal dental restorations.

Comparative analysis of methods for evaluation of stabilometry parameters.

Cluster analysis of stabilometry results in 251 subjects (129 men and 122 women, age 20-60 years) was carried out. Four methods were used in the study: European stabilometry variant, Romberg test (European variant), Romberg's test (American variant), and Romberg's test (American Universal variant). Cluster analysis has shown virtually the same diagnostic informative value of all 4 methods.

Content of matrix metalloproteinase-8 and matrix metalloproteinase-9 in oral fluid of patients with chronic generalized periodontitis.

The content of MMP-8 and MMP-9 in oral fluid of 145 patients (95 women and 50 men, 18-52 years) was measured by enzyme immunoassay. We examined 63 subjects with the intact periodontium and 82 patients with chronic generalized periodontitis (25 patients with mild disease, 45 patients with moderate disease, and 12 patients with severe disease). All patients were examined during the remission of chronic periodontitis and did not have clinical signs of associated somatic diseases.

Serum sFas, leptin, and VEGF in patients with ovarian cancer and benign tumors.

The initial levels of soluble Fas antigen (sFas), leptin, and vascular endothelium growth factor (VEGF) were measured in the sera of 100 patients with ovarian cancer and benign tumors and in 60 healthy women aged 28-65 years. Serum levels of sFas and VEGF were elevated in the total group of patients with ovarian tumors, while leptin levels were the same as in healthy women. The studied parameters did not depend on the age of patients and healthy women.

Hepatitis B virus genetic typing using mass-spectrometry.

Mini-sequencing with subsequent result registration using MALDI-ToF mass-spectrometry was employed for hepatitis B virus genetic typing in Russian population. This approach was employed for hepatitis B virus genetic typing in HBsAg-positive patients with chronic hepatitis B, hepatitis of combined etiology and hepatic cirrhosis and allowed to show the prevalence of D genotype (83.3%) in all groups of patients.

The epidemiology of bladder cancer in Russia.

This study assessed the epidemiology of bladder cancer in Russia. The available publications in Russian were analysed, as well as information from the database of N. N.

Effect of ultrasound on major salivary glands in rats: dynamics of their functional state.

The effect of ultrasound irradiation focused onto the gonial angle of the mandibular bone (5-10 sessions) on salivary function of the major salivary glands was examined on laboratory rats. Repeated sonication modified the character of salivary secretion cycle. In addition, it qualitatively changed ionic composition of the saliva.

Inversion of stress response reprogramming phenomenon in lipopolysaccharide-stimulated alveolar macrophages.

The stress response and NO production in reprogrammed proinflammatory or antiinflammatory alveolar macrophages were studied after lipopolysaccharide treatment. Experiments with macrophages not containing HSP70 showed that lipopolysaccharide in a dose of 500 ng/ml induced stress response in cells with the proinflammatory phenotype (as distinct from an antiinflammatory phenotype). The stress response was not observed in HSP70-containing lipopolysaccharide-stimulated proinflammatory macrophages, but occurred in cells with antiinflammatory phenotype.

Management of urethrovaginal fistulae.

Purpose Of Review: Urethrovaginal fistulae are a rare condition. It is a conceptual mistake to consider urethrovaginal fistulae to be synonymous with vesicovaginal fistulae. Urethrovaginal fistulae are a different entity requiring special attention and treatment.

Management of radiation-induced vesicovaginal fistula.

Background: Pelvic radiation used for the treatment of malignant diseases is the primary cause of delayed vesicovaginal fistula.

Objective: We consider the Martius labial fatty flap technique and the Latzko upper colpocleisis as important tools for the urologist dealing with patients suffering from radiation-induced vesicovaginal fistula (RVVF).

Design, Setting, And Participants: In our clinic, 216 patients with RVVF underwent surgical treatment.

Intracellular and extracellular NO differentially modulates the stress response and apoptosis in macrophages exposed to the influence of biological or physical factors.

We studied the role of extracellular and intracellular NO in the regulation of the stress response and apoptosis in macrophages of proinflammatory and antiinflammatory phenotypes under the influence of S. aureus and heat shock. Blockade of extracellular nitric oxide synthesis in cells with antiinflammatory phenotype inhibited the stress response induced by S.

The fine branches of the human trigeminal nerve.

The fine branches of the trigeminal ganglion were studied by macromicropreparation and total clearing in glycerol of specimens collected during autopsies of 78 people aged 23-69 years impregnated with silver nitrate by the Christensen method or stained with the Schiff reagent as described by Shubich and Khodos; serial histotopograms prepared in the sagittal plane from specimens from 15 autopsies of humans of the same age range and also impregnated by the Christensen method were also studied. Four histotopograms were used for reconstruction of the fine nerves. The results showed that numerous branches of diameter 50-150 microm ran directly from the trigeminal nerve, more from the outer and less from the deeper surface, innervating the walls of the trigeminal cavity.

Transcription factors NFAT2 and Egr1 cooperatively regulate the maturation of T-lymphoma in vitro.

We have demonstrated that transcription factors Egr1 and NFAT2 cooperate in regulation of the early stages of T-lymphocyte development, whereas the related factors Egr2 and Egr3 do not cooperate with NFAT2. Egr1 and NFAT2 are shown to cooperatively control gene expression of the regulatory factor Id3 and recombinase Rag2, whose functions are critical for T-lymphocyte differentiation. Thus, the concerted action of the transcription factors Egr1 and NFAT2 can play a crucial role in regulation of the T cell differentiation in vitro due to the cooperative regulation of Id3 and Rag2 gene expression.

Mathematical model of little invasive interventions on the hollow organs using traditional and shape memory Ni-Ti-based biocompatible superelastic materials.

Comparative analysis of the mode of deformation of hollow organs (extrahepatic bile ducts at the site of stricture) was carried out by the finite elements method during interventions in the lumen using stone extraction Dormia basket made of stainless steel and NiTi. Geometrical and physical models of the ducts and basket were created, strain and deformation fields in the choledochal walls were determined, criteria of traumaticity and medical technological requirements to the material and geometry of lithoextractors were formulated.

A method for evaluating the fluctuations in the intensity of substance transport between blood and bones in the predominant direction.

The dynamics of fluctuations in the intensity of the blood-bone flow of substances in the predominant and opposite directions was evaluated using the percent radioactivity difference coefficient and its graphic presentation. The technology of calculating this coefficient is described.

Sergey S. Korsakov and the beginning of Russian psychiatry.

Sergey Sergeievich Korsakov (1854-1900) was an outstanding Russian psychiatrist, founder of the Moscow psychiatric school, a talented clinician and teacher, and a supporter of the nosological approach in the understanding and systematization of psychiatric illnesses. He described alcoholic polyneuritis with distinctive mental symptoms, which later on was coined "Korsakov's disease." He was the first to give a clear account of paranoia.

A new method for evaluation of radioactive label transport intensity in the predominant direction between blood and liver.

The intensity of (75)Se transport in the predominant direction after intraperitoneal injection of [(75)Se]selenate was compared in 1- and 3-month-old rats receiving common vivarium ration or sucrose diet. The incorporation percent, blood/liver relative radioactivity, and relative radioactivity difference coefficient were evaluated in the blood and liver. The dynamics of label incorporation in the blood of rats fed common diets has two peaks (at 1-3 h and 12-24 h) and a drop at 6 h.

Crystallization of oral fluid components in patients with type 1 diabetes mellitus.

We systematized and described morphological criteria characterizing crystalline aggregates in mixed saliva from patients with type 1 diabetes mellitus.

Morphological changes in submandibular lymph nodes after subcutaneous implantation of gold alloys.

Functional activity of cells in regional lymph nodes of the major salivary glands was studied in rats after subcutaneous implantation of gold alloys. Alloy 900 modified the function of regional lymph nodes.

Crystallization of oral fluid components in patients with type 2 diabetes mellitus.

Crystal aggregations of oral fluid from normal subjects and patients with type 2 diabetes mellitus were examined. Morphological signs characterizing crystal aggregations of salivary pools from patients with type 2 diabetes mellitus are described and classified.

Experimental assessment of biological compatibility of gold alloys by functional indices of salivary glands.

The effect of implanted gold alloys on composition and rate of excretion of oral fluid was studied on rats. It was found that gold alloy 900 modifies the function of the major salivary glands.

Effects of selenium-containing compounds and their metabolism in intact rats and in animals with bone fractures.

Blood content of MDA in rats increased 1 and 2 weeks after mandibular bone fracture at stages of cellular fibrous and chondroid callus and decreased 4 weeks after fracture at the stage of primary bone callus. Treatment with Se (intragastrically and electrophoretically) reduced this increase by activating selenium-containing glutathione peroxidase. In order to clear out the relationship between Se and carbohydrate metabolism in different ages, the distribution of Se between the blood and mandibular bone, diaphysis and metaepiphyseal zone of the femoral bone was studied using the bone/blood relative radioactivity coefficient after intraperitoneal injection of [(75)Se]selenate.

Labeled citrate metabolism in bone fractures and impaired innervation.

Changes in (14)C incorporation into regenerate after bone fracture and impairment of mandibular innervation, and injection of [3-(14)C]cytrate corresponded to the stages of reparative osteogenesis: after 1 week (14)C incorporation in the cellular-fibrous callus surpassed its release, after 2 weeks the rates of (14)C incorporation and release in the chondroid callus become similar, and after 4 weeks the release of the label predominated in the primary bone callus. Denervation reduced (14)C incorporation into regenerate, which impaired bone remodeling. Citrate in the bones is characterized by high metabolic activity.

[Morphological study of histiocytosis from Langerhans cells in jaw bones].

Histiocytosis of the jaws in biopsies from 17 patients aged 19 to 51 years were studied at light and electron microscopy level. Birbek's bodies were found in Langerhans cells in all the biopsies confirming the diagnosis of Langerhans cell histiocytoma. The structure of Birbek's bodies changed depending on the treatment--radiation or chemotherapy.

[Magnetic resonance imaging and ultrasound study in the diagnosis og facial angiodysplasias in children: competition or collaboration?].

This study deals with the capacities of noninvasive magnetic resonance imaging (MRI) with magnetic resonance angiography (MRA) without administration of magnetic resonance contrast substances in the diagnosis of facial soft tissue angiodysplasias in children. Indications for their use are defined. The methodology of the studies is proposed.